LOCUS       40924_4234        3126 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAJM.716, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Marionette: E. coli containing 12 highly-optimized small molecule 
            sensors
  JOURNAL   bioRxivorg 285866, 1-26 (2018)
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2018) Synthetic Biology Center, Department of 
            Biological Engineering, Massachusetts Institute of Technology, 77 
            Massachusetts Avenue, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 3126)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3126)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxivorg 
            285866, 1-26 (2018)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-MAR-2018) Synthetic Biology Center, Department of Biological 
            Engineering, Massachusetts Institute of Technology, 77 Massachusetts
            Avenue, Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..53
                     /label=L3S3P21
                     /note="L3S3P21"
                     /regulatory_class="terminator"
     promoter        72..356
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     regulatory      357
                     /label=+1
                     /note="+1"
                     /regulatory_class="other"
     misc_RNA        363..413
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      445..456
                     /label=B0064
                     /note="B0064"
                     /regulatory_class="ribosome_binding_site"
     CDS             463..1179
                     /label=EYFP
                     /note="enhanced YFP"
     regulatory      1183..1243
                     /label=L3S2P21
                     /note="L3S2P21"
                     /regulatory_class="terminator"
     rep_origin      complement(1577..2122)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(2214..3026)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac
       61 gatcgttggc tgagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt
      121 ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca ttctgtaaca
      181 aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa
      241 agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat
      301 aagattagcg gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc
      361 gagctgtcac cggatgtgct ttccggtctg atgagtccgt gaggacgaaa cagcctctac
      421 aaataatttt gtttaatact agagaaagag gggaaatact agatggtgag caagggcgag
      481 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      541 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      601 ttcatctgca ccacaggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc
      661 tacggcctgc aatgcttcgc ccgctacccc gaccacatga agctgcacga cttcttcaag
      721 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      781 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      841 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
      901 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
      961 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1021 accccaatcg gcgacggccc cgtgctgctg cccgacaacc actaccttag ctaccagtcc
     1081 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1141 gccgccggga tcactctcgg catggacgag ctgtacaagt aactcggtac caaattccag
     1201 aaaagaggcc tcccgaaagg ggggcctttt ttcgttttgg tccaatggcg gcgcgccatc
     1261 gaatgaggtg cttcctcgct cactgactcg ctgcacgagg cagacctcag cgctagcgga
     1321 gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc
     1381 aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct
     1441 tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac
     1501 gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg
     1561 ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
     1621 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1681 gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     1741 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     1801 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac
     1861 tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt
     1921 aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac
     1981 aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca
     2041 gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac
     2101 gcgcagacca aaacgatctc aagaagatca tcttattaag gggtctgacg ctcagtggaa
     2161 cgaaaaatca atctaaagta tatatgagta aacttggtct gacagttacc ttagaaaaac
     2221 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
     2281 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
     2341 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
     2401 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
     2461 gagaatggca aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc
     2521 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
     2581 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
     2641 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
     2701 acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta
     2761 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
     2821 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
     2881 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga
     2941 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa
     3001 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
     3061 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtacatcag agattttgag
     3121 acacaa
//
