LOCUS       V009547                 3816 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009547
VERSION     V009547
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Marionette: E. coli containing 12 highly-optimized small molecule
            sensors
  JOURNAL   bioRxivorg 285866, 1-26 (2018)
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2018) Synthetic Biology Center, Department of
            Biological Engineering, Massachusetts Institute of Technology, 77
            Massachusetts Avenue, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 3816)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3816)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxivorg
            285866, 1-26 (2018)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-MAR-2018) Synthetic Biology Center, Department of Biological
            Engineering, Massachusetts Institute of Technology, 77 Massachusetts
            Avenue, Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..53
                     /label="L3S3P21"
                     /note="L3S3P21"
                     /regulatory_class="terminator"
     regulatory      73..135
                     /label="PBetI"
                     /note="PBetI"
                     /regulatory_class="promoter"
     regulatory      101..106
                     /regulatory_class="minus_35_signal"
     misc_feature    107..128
                     /label="BetO operator"
                     /note="BetO operator"
     regulatory      124..129
                     /regulatory_class="minus_10_signal"
     misc_RNA        137..187
                     /label="sTRSV HHRz"
                     /note="hammerhead ribozyme from the tobacco ringspot virus
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      219..230
                     /label="B0064"
                     /note="B0064"
                     /regulatory_class="ribosome_binding_site"
     CDS             237..953
                     /label="EYFP"
                     /note="enhanced YFP"
     regulatory      957..1017
                     /label="L3S2P21"
                     /note="L3S2P21"
                     /regulatory_class="terminator"
     regulatory      1022..1075
                     /label="PLacIQ"
                     /note="PLacIQ"
                     /regulatory_class="promoter"
     regulatory      1040..1045
                     /regulatory_class="minus_35_signal"
     regulatory      1063..1068
                     /regulatory_class="minus_10_signal"
     regulatory      1076..1103
                     /label="bet2"
                     /note="bet2"
                     /regulatory_class="ribosome_binding_site"
     CDS             1104..1688
                     /gene="betI"
                     /label="HTH-type transcriptional regulator BetI"
                     /note="HTH-type transcriptional regulator BetI from
                     Escherichia coli (strain K12). Accession#: P17446"
     regulatory      1701..1955
                     /label="induction operon terminator region"
                     /note="induction operon terminator region"
                     /regulatory_class="terminator"
     rep_origin      complement(2267..2812)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(2904..3716)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac
       61 gatcgttggc tgagcgcggg tgagagggat tcgttaccaa tagacaattg attggacgtt
      121 caatataatg ctagcagctg tcaccggatg tgctttccgg tctgatgagt ccgtgaggac
      181 gaaacagcct ctacaaataa ttttgtttaa tactagagaa agaggggaaa tactagatgg
      241 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      301 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      361 agctgaccct gaagttcatc tgcaccacag gcaagctgcc cgtgccctgg cccaccctcg
      421 tgaccacctt cggctacggc ctgcaatgct tcgcccgcta ccccgaccac atgaagctgc
      481 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      541 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      601 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
      661 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
      721 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
      781 actaccagca gaacacccca atcggcgacg gccccgtgct gctgcccgac aaccactacc
      841 ttagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      901 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaactcg
      961 gtaccaaatt ccagaaaaga ggcctcccga aaggggggcc ttttttcgtt ttggtccaat
     1021 ggcggcgcgc catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag
     1081 agagtcaatt cacgggggtg aatatgccga aactgggtat gcagagcatt cgtcgtcgtc
     1141 agctgattga tgcaaccctg gaagcaatta atgaagttgg tatgcatgat gcaaccattg
     1201 cacagattgc acgtcgtgcc ggtgttagca ccggtattat tagccattat ttccgcgata
     1261 aaaacggtct actggaagca accatgcgtg atattaccag ccagctgcgt gatgcagttc
     1321 tgaatcgtct gcatgcactg ccgcagggta gcgcagaaca gcgtctgcag gcaattgttg
     1381 gtggtaattt tgatgaaacc caggttagca gcgcagcaat gaaagcatgg ctggcatttt
     1441 gggcaatcag catgcatcag ccgatgctgt atcgtctgca gcaggttagc agtcgtcgtc
     1501 tgctgagcaa tctggttagc gaatttcgtc gtgaactgcc tcgtgaacag gcacaagagg
     1561 caggttatgg tctggcagca ctgattgatg gtctgtggct gcgtgcagca ctgagcggta
     1621 aaccgctgga taaaacccgt gcaaatagcc tgacccgtca ttttatcacc cagcatctgc
     1681 cgaccgattg ataaggatcc taattggtaa cgaatcagac aattgacggc tcgagggagt
     1741 agcatagggt ttgcagaatc cctgcttcgt ccatttgaca ggcacattat gcatcgatga
     1801 taagctgtca aacatgagca gatcctctac gccggacgca tcgtggccgg catcaccggc
     1861 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
     1921 cgccacttcg ggctcatgag caaatatttt atctgaggtg cttcctcgct cactgactcg
     1981 ctgcacgagg cagacctcag cgctagcgga gtgtatactg gcttactatg ttggcactga
     2041 tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag
     2101 cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc
     2161 gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc
     2221 aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc
     2281 gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag
     2341 gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct cctgttcctg
     2401 cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg
     2461 acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt
     2521 cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat
     2581 gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc
     2641 atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca
     2701 gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc
     2761 ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca
     2821 tcttattaag gggtctgacg ctcagtggaa cgaaaaatca atctaaagta tatatgagta
     2881 aacttggtct gacagttacc ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
     2941 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
     3001 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
     3061 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
     3121 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc
     3181 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     3241 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
     3301 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     3361 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca
     3421 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     3481 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     3541 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt
     3601 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     3661 atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc gttgaatatg gctcataaca
     3721 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
     3781 tcttgtgcaa tgtacatcag agattttgag acacaa
//
