LOCUS       V009548                 5793 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009548
VERSION     V009548
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Marionette: E. coli containing 12 highly-optimized small molecule
            sensors
  JOURNAL   bioRxivorg 285866, 1-26 (2018)
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2018) Synthetic Biology Center, Department of
            Biological Engineering, Massachusetts Institute of Technology, 77
            Massachusetts Avenue, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 5793)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5793)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxivorg
            285866, 1-26 (2018)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-MAR-2018) Synthetic Biology Center, Department of Biological
            Engineering, Massachusetts Institute of Technology, 77 Massachusetts
            Avenue, Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..53
                     /label="L3S3P21"
                     /note="L3S3P21"
                     /regulatory_class="terminator"
     promoter        72..356
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     misc_RNA        363..413
                     /label="sTRSV HHRz"
                     /note="hammerhead ribozyme from the tobacco ringspot virus
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      445..456
                     /label="B0064"
                     /note="B0064"
                     /regulatory_class="ribosome_binding_site"
     CDS             463..1179
                     /label="EYFP"
                     /note="enhanced YFP"
     regulatory      1183..1243
                     /label="L3S2P21"
                     /note="L3S2P21"
                     /regulatory_class="terminator"
     regulatory      1248..1301
                     /label="PLacIQ"
                     /note="PLacIQ"
                     /regulatory_class="promoter"
     regulatory      1266..1271
                     /regulatory_class="minus_35_signal"
     regulatory      1289..1294
                     /regulatory_class="minus_10_signal"
     regulatory      1302..1329
                     /label="ara1"
                     /note="ara1"
                     /regulatory_class="ribosome_binding_site"
     CDS             1330..2205
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     regulatory      2209..2249
                     /label="e1"
                     /note="e1"
                     /regulatory_class="ribosome_binding_site"
     CDS             2250..3665
                     /gene="araE"
                     /label="Arabinose-proton symporter"
                     /note="Arabinose-proton symporter from Escherichia coli
                     (strain K12). Accession#: P0AE24"
     regulatory      3678..3932
                     /label="induction operon terminator region"
                     /note="induction operon terminator region"
                     /regulatory_class="terminator"
     rep_origin      complement(4244..4789)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(4881..5693)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac
       61 gatcgttggc tgagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt
      121 ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca ttctgtaaca
      181 aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa
      241 agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat
      301 aagattagcg gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc
      361 gagctgtcac cggatgtgct ttccggtctg atgagtccgt gaggacgaaa cagcctctac
      421 aaataatttt gtttaatact agagaaagag gggaaatact agatggtgag caagggcgag
      481 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      541 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      601 ttcatctgca ccacaggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc
      661 tacggcctgc aatgcttcgc ccgctacccc gaccacatga agctgcacga cttcttcaag
      721 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      781 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      841 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
      901 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
      961 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1021 accccaatcg gcgacggccc cgtgctgctg cccgacaacc actaccttag ctaccagtcc
     1081 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1141 gccgccggga tcactctcgg catggacgag ctgtacaagt aactcggtac caaattccag
     1201 aaaagaggcc tcccgaaagg ggggcctttt ttcgttttgg tccaatggcg gcgcgccatc
     1261 gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg
     1321 gtggtgaata tggctgaagc gcaaaatgat cccctgctgc cgggatactc gtttaatgcc
     1381 catctggtgg cgggtttaac gccgattgag gccaacggtt atctcgattt ttttatcgac
     1441 cgaccgctgg gaatgaaagg ttatattctc aatctcacca ttcgcggtca gggggtggtg
     1501 aaaaatcagg gacgagaatt tgtttgccga ccgggtgata ttttgctgtt cccgccagga
     1561 gagattcatc actacggtcg tcatccggag gctcgcgaat ggtatcacca gtgggtttac
     1621 tttcgtccgc gcgcctactg gcatgaatgg cttaactggc cgtcaatatt tgccaatacg
     1681 gggttctttc gcccggatga agcgcaccag ccgcatttca gcgacttttt tgggcaaatc
     1741 attaacgccg ggcaagggga agggcgctat tcggagctgc tggcgataaa tctgcttgag
     1801 caattgttac tgcggcgcat gctagcgatt aacggatcgc tccatccacc gatggataat
     1861 cgggtacgcg aggcttgtca gtacatcagc gatcacctgg cagacagcaa ttttgatatc
     1921 gccagcgtcg cacagcatgt ttgcttgtcg ccgtcgcgtc tgtcacatct tttccgccag
     1981 cagttaggga ttagcgtctt aagctggcgc gaggaccaac gtatcagcca ggcgaagctg
     2041 cttttgagca ccacccggat gcctatcgcc accgtcggtc gcaatgttgg ttttgacgat
     2101 caactctatt tctcgcgggt atttaaaaaa tgcaccgggg ccagcccgag cgagttccgt
     2161 gccggtttgg aagaaaaagt gaatgatgta gccgtcaagt tgtcatgata agatcctatt
     2221 ccagcgggat taaagaggag cgattaagca tggttactat caatacggaa tctgctttaa
     2281 cgccacgttc tttgcgggat acgcggcgta tgaatatgtt tgtttcggta gctgctgcgg
     2341 tcgcaggatt gttatttggt cttgatatcg gcgtaatcgc cggagcgttg ccgttcatta
     2401 ccgatcactt tgtgctgacc agtcgtttgc aggaatgggt ggttagtagc atgatgctcg
     2461 gtgcagcaat tggtgcgctg tttaatggtt ggctgtcgtt ccgcctgggg cgtaaataca
     2521 gcctgatggc gggggccatc ctgtttgtac tcggttctat agggtccgct tttgcgacca
     2581 gcgtagagat gttaatcgcc gctcgtgtgg tgctgggcat tgctgtcggg atcgcgtctt
     2641 acaccgctcc tctgtatctt tctgaaatgg caagtgaaaa cgttcgcggt aagatgatca
     2701 gtatgtacca gttgatggtc acactcggca tcgtgctggc gtttttatcc gatacagcgt
     2761 tcagttatag cggtaactgg cgcgcaatgt tgggggttct tgctttacca gcagttctgc
     2821 tgattattct ggtagtattt ctgccaaata gcccgcgctg gctggcggaa aaggggcgtc
     2881 atattgaggc ggaagaagta ttgcgtatgc tgcgcgatac gtcggaaaaa gcgcgagaag
     2941 aactcaacga aattcgtgaa agcctgaagt taaaacaggg cggttgggca ctgtttaaga
     3001 tcaaccgtaa cgtccgtcgt gctgtgtttc tcggtatgtt gttgcaggcg atgcagcagt
     3061 ttaccggtat gaacatcatc atgtactacg cgccgcgtat cttcaaaatg gcgggcttta
     3121 cgaccacaga acaacagatg attgcgactc tggtcgtagg gctgaccttt atgttcgcca
     3181 cctttattgc ggtgtttacg gtagataaag cagggcgtaa accggctctg aaaattggtt
     3241 tcagcgtgat ggcgttaggc actctggtgc tgggctattg cctgatgcag tttgataacg
     3301 gtacggcttc cagtggcttg tcctggctct ctgttggcat gacgatgatg tgtattgccg
     3361 gttatgcgat gagcgccgcg ccagtggtgt ggatcctgtg ctctgaaatt cagccgctga
     3421 aatgccgcga tttcggtatt acctgttcga ccaccacgaa ctgggtgtcg aatatgatta
     3481 tcggcgcgac cttcctgaca ctgcttgata gcattggcgc tgccggtacg ttctggctct
     3541 acactgcgct gaacattgcg tttgtgggca ttactttctg gctcattccg gaaaccaaaa
     3601 atgtcacgct ggaacatatc gaacgcaaac tgatggcagg cgagaagttg agaaatatcg
     3661 gcgtctgata aggatcctaa ttggtaacga atcagacaat tgacggctcg agggagtagc
     3721 atagggtttg cagaatccct gcttcgtcca tttgacaggc acattatgca tcgatgataa
     3781 gctgtcaaac atgagcagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
     3841 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
     3901 cacttcgggc tcatgagcaa atattttatc tgaggtgctt cctcgctcac tgactcgctg
     3961 cacgaggcag acctcagcgc tagcggagtg tatactggct tactatgttg gcactgatga
     4021 gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag
     4081 aatatgtgat acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt
     4141 cgactgcggc gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg
     4201 aagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc
     4261 cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac
     4321 tataaagata ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct
     4381 ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca
     4441 ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag
     4501 tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca
     4561 aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg
     4621 cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt
     4681 acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt
     4741 tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct
     4801 tattaagggg tctgacgctc agtggaacga aaaatcaatc taaagtatat atgagtaaac
     4861 ttggtctgac agttacctta gaaaaactca tcgagcatca aatgaaactg caatttattc
     4921 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     4981 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     5041 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     5101 tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag
     5161 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     5221 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa
     5281 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     5341 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg
     5401 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     5461 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     5521 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
     5581 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     5641 ttggaattta atcgcggcct cgagcaagac gtttcccgtt gaatatggct cataacaccc
     5701 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
     5761 tgtgcaatgt acatcagaga ttttgagaca caa
//
