LOCUS       V009549                 3852 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009549
VERSION     V009549
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Marionette: E. coli containing 12 highly-optimized small molecule
            sensors
  JOURNAL   bioRxivorg 285866, 1-26 (2018)
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2018) Synthetic Biology Center, Department of
            Biological Engineering, Massachusetts Institute of Technology, 77
            Massachusetts Avenue, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 3852)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3852)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxivorg
            285866, 1-26 (2018)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-MAR-2018) Synthetic Biology Center, Department of Biological
            Engineering, Massachusetts Institute of Technology, 77 Massachusetts
            Avenue, Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..53
                     /label="L3S3P21"
                     /note="L3S3P21"
                     /regulatory_class="terminator"
     regulatory      73..123
                     /label="PTtg"
                     /note="PTtg"
                     /regulatory_class="promoter"
     misc_feature    83..110
                     /label="TtgO operator"
                     /note="TtgO operator"
     regulatory      85..90
                     /regulatory_class="minus_35_signal"
     regulatory      109..114
                     /regulatory_class="minus_10_signal"
     regulatory      121
                     /label="+1"
                     /note="+1"
                     /regulatory_class="other"
     misc_RNA        125..175
                     /label="sTRSV HHRz"
                     /note="hammerhead ribozyme from the tobacco ringspot virus
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      207..218
                     /label="B0064"
                     /note="B0064"
                     /regulatory_class="ribosome_binding_site"
     CDS             225..941
                     /label="EYFP"
                     /note="enhanced YFP"
     regulatory      945..1005
                     /label="L3S2P21"
                     /note="L3S2P21"
                     /regulatory_class="terminator"
     regulatory      1010..1063
                     /label="PLacIQ"
                     /note="PLacIQ"
                     /regulatory_class="promoter"
     regulatory      1028..1033
                     /regulatory_class="minus_35_signal"
     regulatory      1051..1056
                     /regulatory_class="minus_10_signal"
     regulatory      1064..1091
                     /label="ttg2"
                     /note="ttg2"
                     /regulatory_class="ribosome_binding_site"
     CDS             1092..1721
                     /gene="ttgR"
                     /label="HTH-type transcriptional regulator TtgR"
                     /note="HTH-type transcriptional regulator TtgR from
                     Pseudomonas putida (strain DOT-T1E). Accession#: Q9AIU0"
     regulatory      1737..1991
                     /label="induction operon terminator region"
                     /note="induction operon terminator region"
                     /regulatory_class="terminator"
     rep_origin      complement(2303..2848)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(2940..3752)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac
       61 gatcgttggc tgcacccagc agtatttaca aacaaccatg aatgtaagta tattccttag
      121 caaagctgtc accggatgtg ctttccggtc tgatgagtcc gtgaggacga aacagcctct
      181 acaaataatt ttgtttaata ctagagaaag aggggaaata ctagatggtg agcaagggcg
      241 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      301 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      361 agttcatctg caccacaggc aagctgcccg tgccctggcc caccctcgtg accaccttcg
      421 gctacggcct gcaatgcttc gcccgctacc ccgaccacat gaagctgcac gacttcttca
      481 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      541 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      601 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      661 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
      721 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
      781 acaccccaat cggcgacggc cccgtgctgc tgcccgacaa ccactacctt agctaccagt
      841 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
      901 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaactcggt accaaattcc
      961 agaaaagagg cctcccgaaa ggggggcctt ttttcgtttt ggtccaatgg cggcgcgcca
     1021 tcgaatggtg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca
     1081 gggaggtgaa tatggttcgt cgtaccaaag aagaggcaca agaaacccgt gcacagatta
     1141 ttgaagcagc agaacgtgca ttctataaac gtggtgttgc acgtaccacc ctggcagata
     1201 ttgcagaact ggcaggcgtt acccgtggtg caatttattg gcattttaac aacaaagccg
     1261 aactggttca ggcactgctg gatagcctgc atgaaaccca tgatcatctg gcacgtgcaa
     1321 gcgaaagcga agatgaagtt gatccgctgg gttgtatgcg taaactgctg ctgcaggttt
     1381 ttaatgaact ggttctggat gcacgtaccc gtcgtattaa tgaaatcctg catcacaaat
     1441 gcgagttcac cgatgatatg tgtgaaattc gtcagcagca tcagagcgca gttctggatt
     1501 gtcataaagg tattaccctg acactggcaa atgtagttcg tcgtggtcag ctgcctggtg
     1561 aactggatgc agaacgtgcc gcagttgcaa tgtttgccta tgttgatggt ctgattcgtc
     1621 gttggctgct gctgccggat agcgttgatc tgctgggtga tgttgaaaaa tgggttgata
     1681 ccggtctgga tatgctgcgt ctgagtccgg cactgcgtaa ataatgataa ggatcctaat
     1741 tggtaacgaa tcagacaatt gacggctcga gggagtagca tagggtttgc agaatccctg
     1801 cttcgtccat ttgacaggca cattatgcat cgatgataag ctgtcaaaca tgagcagatc
     1861 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     1921 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcaaa
     1981 tattttatct gaggtgcttc ctcgctcact gactcgctgc acgaggcaga cctcagcgct
     2041 agcggagtgt atactggctt actatgttgg cactgatgag ggtgtcagtg aagtgcttca
     2101 tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat
     2161 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg
     2221 gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg
     2281 agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa
     2341 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2401 cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
     2461 cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt
     2521 cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc
     2581 ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc
     2641 actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga
     2701 aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt
     2761 agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga
     2821 gattacgcgc agaccaaaac gatctcaaga agatcatctt attaaggggt ctgacgctca
     2881 gtggaacgaa aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccttag
     2941 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     3001 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     3061 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     3121 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     3181 tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca
     3241 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     3301 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     3361 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     3421 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
     3481 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     3541 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     3601 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     3661 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc
     3721 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     3781 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta catcagagat
     3841 tttgagacac aa
//
