LOCUS       V009551                 3973 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009551
VERSION     V009551
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Marionette: E. coli containing 12 highly-optimized small molecule
            sensors
  JOURNAL   bioRxivorg 285866, 1-26 (2018)
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2018) Synthetic Biology Center, Department of
            Biological Engineering, Massachusetts Institute of Technology, 77
            Massachusetts Avenue, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 3973)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3973)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxivorg
            285866, 1-26 (2018)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-MAR-2018) Synthetic Biology Center, Department of Biological
            Engineering, Massachusetts Institute of Technology, 77 Massachusetts
            Avenue, Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..53
                     /label="L3S3P21"
                     /note="L3S3P21"
                     /regulatory_class="terminator"
     regulatory      73..127
                     /label="PLuxB"
                     /note="PLuxB"
                     /regulatory_class="promoter"
     misc_feature    73..92
                     /label="LuxO operator"
                     /note="LuxO operator"
     regulatory      93..98
                     /regulatory_class="minus_35_signal"
     regulatory      114..119
                     /regulatory_class="minus_10_signal"
     misc_RNA        129..179
                     /label="sTRSV HHRz"
                     /note="hammerhead ribozyme from the tobacco ringspot virus
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      211..222
                     /label="B0064"
                     /note="B0064"
                     /regulatory_class="ribosome_binding_site"
     CDS             229..945
                     /label="EYFP"
                     /note="enhanced YFP"
     regulatory      949..1009
                     /label="L3S2P21"
                     /note="L3S2P21"
                     /regulatory_class="terminator"
     regulatory      1014..1067
                     /label="PLacI"
                     /note="PLacI"
                     /regulatory_class="promoter"
     regulatory      1032..1037
                     /regulatory_class="minus_35_signal"
     regulatory      1055..1060
                     /regulatory_class="minus_10_signal"
     regulatory      1068..1095
                     /label="lux1"
                     /note="lux1"
                     /regulatory_class="ribosome_binding_site"
     CDS             1096..1845
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     regulatory      1858..2112
                     /label="induction operon terminator region"
                     /note="induction operon terminator region"
                     /regulatory_class="terminator"
     rep_origin      complement(2424..2969)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(3061..3873)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac
       61 gatcgttggc tgacctgtag gatcgtacag gtttacgcaa gaaaatggtt tgttacagtc
      121 gaataaaagc tgtcaccgga tgtgctttcc ggtctgatga gtccgtgagg acgaaacagc
      181 ctctacaaat aattttgttt aatactagag aaagagggga aatactagat ggtgagcaag
      241 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      301 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      361 ctgaagttca tctgcaccac aggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      421 ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct gcacgacttc
      481 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      541 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      601 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      661 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
      721 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
      781 cagaacaccc caatcggcga cggccccgtg ctgctgcccg acaaccacta ccttagctac
      841 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      901 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaact cggtaccaaa
      961 ttccagaaaa gaggcctccc gaaagggggg ccttttttcg ttttggtcca atggcggcgc
     1021 gccatcgaat ggcgcaaaac ctttcgcggt atggcatgat agcgcccgga agagagtcaa
     1081 ttcagggtgg tgaatatgaa aaacataaat gccgacgaca catacagaat aattaataaa
     1141 attaaagctt gtagaagcaa taatgatatt aatcaatgct tatctgatat gactaaaatg
     1201 gtacattgtg aatattattt actcgcgatc atttatcctc attctatggt taaatctgat
     1261 atttcaatcc tagataatta ccctaaaaaa tggaggcaat attatgatga cgctaattta
     1321 ataaaatatg atcctatagt agattattct aactccaatc attcaccaat taattggaat
     1381 atatttgaaa acaatgctgt aaataaaaaa tctccaaatg taattaaaga agcgaaaaca
     1441 tcaggtctta tcactgggtt tagtttccct attcatacgg ctaacaatgg cttcggaatg
     1501 cttagttttg cacattcaga aaaagacaac tatatagata gtttattttt acatgcgtgt
     1561 atgaacatac cattaattgt tccttctcta gttgataatt atcgaaaaat aaatatagca
     1621 aataataaat caaacaacga tttaaccaaa agagaaaaag aatgtttagc gtgggcatgc
     1681 gaaggaaaaa gctcttggga tatttcaaaa atattaggtt gcagtgagcg tactgtcact
     1741 ttccatttaa ccaatgcgca aatgaaactc aatacaacaa accgctgcca aagtatttct
     1801 aaagcaattt taacaggagc aattgattgc ccatacttta aaaattgata aggatcctaa
     1861 ttggtaacga atcagacaat tgacggctcg agggagtagc atagggtttg cagaatccct
     1921 gcttcgtcca tttgacaggc acattatgca tcgatgataa gctgtcaaac atgagcagat
     1981 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
     2041 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcaa
     2101 atattttatc tgaggtgctt cctcgctcac tgactcgctg cacgaggcag acctcagcgc
     2161 tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt gaagtgcttc
     2221 atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat acaggatata
     2281 ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggaaat
     2341 ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta acagggaagt
     2401 gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa
     2461 atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2521 ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt
     2581 ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt
     2641 tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc
     2701 cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc
     2761 cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg
     2821 aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg
     2881 tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag
     2941 agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaagggg tctgacgctc
     3001 agtggaacga aaaatcaatc taaagtatat atgagtaaac ttggtctgac agttacctta
     3061 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc
     3121 atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag
     3181 gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat
     3241 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga
     3301 atccggtgag aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc
     3361 attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc
     3421 ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg
     3481 caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc
     3541 ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc
     3601 aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag
     3661 tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa
     3721 ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt
     3781 atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct
     3841 cgagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta
     3901 agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt acatcagaga
     3961 ttttgagaca caa
//
