LOCUS       40924_4174        7720 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Yeast expression vector pAJ401mds1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7720)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7720)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7720)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      486..1305
                     /label=PGK promoter
                     /note="PGK promoter"
                     /regulatory_class="promoter"
     misc_feature    1341..1672
                     /label=5' UTR mds1
                     /note="5' UTR mds1"
     CDS             1673..3244
                     /codon_start=1
                     /note="unnamed protein product; mds1"
                     /protein_id="SJL87803.1"
                     /translation="MRFPSSSVLALGLIGPALAYPKPGATKRGSPNPTRAAAVKAAFQT
                     SWNAYHHFAFPHDDLHPVSNSFDDERNGWGSSAIDGLDTAILMGDADIVNTILQYVPQI
                     NFTTTAVANQGSSVFETNIRYLGGLLSAYDLLRGPFSSLATNQTLVNSLLRQAQTLANG
                     LKVAFTTPSGVPDPTVFFNPTVRRSGASSNNVAEIGSLVLEWTRLSDLTGNPQYAQLAQ
                     KGESYLLNPKGSPEAWPGLIGTFVSTSNGTFQDSSGSWSGLMDSFYEYLIKMYLYDPVA
                     FAHYKDRWVLGADSTIGHLGSHPSTRKDLTFLSSYNGQSTSPNSGHLASFGGGNFILGG
                     ILLNEQKYIDFGIKLASSYFGTYTQTASGIGPEGFAWVDSVTGAGGSPPSSQSGFYSSA
                     GFWVTAPYYILRPETLESLYYAYRVTGDSKWQDLAWEALSAIEDACRAGSAYSSINDVT
                     QANGGGASDDMESFWFAEALKYAYLIFAEESDVQVQATGGNKFVFNTEAHPFSIRSSSR
                     RGGHLA"
     misc_feature    3245..3490
                     /label=3' UTR mds1
                     /note="3' UTR mds1"
     regulatory      3506..3769
                     /label=PGK terminator
                     /note="PGK terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(3788..3804)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3812..3828)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3836..3866)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3881..3902)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(4109..4909)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(4910..5126)
                     /label=URA3 promoter
     protein_bind    5313..5360
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     rep_origin      complement(5901..6489)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6663..7520)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7521..7625)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ggccgatgcg gccgcaattc
      421 gagctcggta cccggggatc ctctagagtc gataaaaaaa aaaaaaaaaa aaaaaaaaaa
      481 aaaaaattaa tttttttttc tttcctcttt ttattaacct taatttttat tttagattcc
      541 tgacttcaac tcaagacgca cagatattat aacatctgca taataggcat ttgcaagaat
      601 tactcgtgag taaggaaaga gtgaggaact atcgcatacc tgcatttaaa gatgccgatt
      661 tgggcgcgaa tcctttattt tggcttcacc ctcatactat tatcagggcc agaaaaagga
      721 agtgtttccc tccttcttga attgatgtta ccctcataaa gcacgtggcc tcttatcgag
      781 aaagaaatta ccgtcgctcg tgatttgttt gcaaaaagaa caaaactgaa aaaacccaga
      841 cacgctcgac ttcctgtctt cctattgatt gcagcttcca atttcgtcac acaacaaggt
      901 cctagcgacg gctcacaggt tttgtaacaa gcaatcgaag gttctggaat ggcgggaaag
      961 ggtttagtac cacatgctat gatgcccact gtgatctcca gagcaaagtt cgttcgatcg
     1021 tactgttact ctctctcttt caaacagaat tgtccgaatc gtgtgacaac aacagcctgt
     1081 tctcacacac tcttttcttc taaccaaggg ggtggtttag tttagtagaa cctcgtgaaa
     1141 cttacattta catatatata aacttgcata aattggtcaa tgcaagaaat agatatttgg
     1201 tcttttctaa ttcgtagttt ttcaagttct tagatgcttt ctttttctct tttttacaga
     1261 tcatcaagga agtaattatc tactttttac aacaaatata aaaaccaaaa gatctacgta
     1321 ctcgaagaat tcggcacgag cgcatctgca gggggcgcca tgtcttaaat tggaagagga
     1381 ggaattttat gcttcgcatg aggtacccgc ttggccgctt gaagaacaat tcgcattctg
     1441 gtggcgtagg cagtgtcgcg tcgcatgagg gaccccctct tctcattctc ctggctctgc
     1501 tgattccgga ggaggaggag gagcatttga gaaagcattg aaatggacgt ctctcccacc
     1561 cgtggcactt ttatctgcag ccccgtctca tgccttgagc ctgggcaagt gtctctctcc
     1621 tcttcgcctt ggcgtccttg ttgtcaaagc ctgaactaca gctgctgcca acatgagatt
     1681 ccctagcagc tccgtccttg ccctcgggct catcggacct gcgctggcgt atccaaagcc
     1741 gggcgccaca aaacgtggat ctcccaaccc tacgagggcg gcagcagtca aggccgcatt
     1801 ccagacgtcg tggaacgctt accaccattt tgcctttccc catgacgacc tccacccggt
     1861 cagcaacagc tttgatgatg agagaaacgg ctggggctcg tcggcaatcg atggcttgga
     1921 cacggctatc ctcatggggg atgccgacat tgtgaacacg atccttcagt atgtaccgca
     1981 gatcaacttc accacgactg cggttgccaa ccaaggatcc tccgtgttcg agaccaacat
     2041 tcggtacctc ggtggcctgc tttctgccta tgacctgttg cgaggtcctt tcagctcctt
     2101 ggcgacaaac cagaccctgg taaacagcct tctgaggcag gctcaaacac tggccaacgg
     2161 cctcaaggtt gcgttcacca ctcccagcgg tgtcccggac cctaccgtct tcttcaaccc
     2221 tactgtccgg agaagtggtg catctagcaa caacgtcgct gaaattggaa gcctggtgct
     2281 cgagtggaca cggttgagcg acctgacggg aaacccgcag tatgcccagc ttgcgcagaa
     2341 gggcgagtcg tatctcctga atccaaaggg aagcccggag gcatggcctg gcctgattgg
     2401 aacgtttgtc agcacgagca acggtacctt tcaggatagc agcggcagct ggtccggcct
     2461 catggacagc ttctacgagt acctgatcaa gatgtacctg tacgacccgg ttgcgtttgc
     2521 acactacaag gatcgctggg tccttggtgc cgactcgacc attgggcatc tcggctctca
     2581 cccgtcgacg cgcaaggact tgaccttttt gtcttcgtac aacggacagt ctacgtcgcc
     2641 aaactcagga catttggcca gttttggcgg tggcaacttc atcttgggag gcattctcct
     2701 gaacgagcaa aagtacattg actttggaat caagcttgcc agctcgtact ttggcacgta
     2761 cacccagacg gcttctggaa tcggccccga aggcttcgcg tgggtggaca gcgtgacggg
     2821 cgccggcggc tcgccgccct cgtcccagtc cgggttctac tcgtcggcag gattctgggt
     2881 gacggcaccg tattacatcc tgcggccgga gacgctggag agcttgtact acgcataccg
     2941 cgtcacgggc gactccaagt ggcaggacct ggcgtgggaa gcgttgagtg ccattgagga
     3001 cgcatgccgc gccggcagcg cgtactcgtc catcaacgac gtgacgcagg ccaacggcgg
     3061 gggtgcctct gacgatatgg agagcttctg gtttgccgag gcgctcaagt atgcgtacct
     3121 gatctttgcg gaggagtcgg atgtgcaggt gcaggccacc ggcgggaaca aatttgtctt
     3181 taacacggag gcgcacccct ttagcatccg ttcatcatca cgacggggcg gccaccttgc
     3241 ttaaaggcgc tgagagttct ctcgcctgtt tttttgctcg tattattcat tttctgctgc
     3301 tcgtcccgaa tcgctccgta gatattttga cacatgcgtc tggatagagc ggctgggacc
     3361 accgaatatg gtatgtcatt cgagatatga agcgcaagag atctttaaga tccaagttct
     3421 ctggtattat actggatgag atagatctat aggaaaccct gtccgaattg tgtggaaaaa
     3481 aaaaaaaaaa nnnctcgagt aattccgata gatcaatttt tttcttttct ctttccccat
     3541 cctttacgct aaaataatag tttattttat tttttgaata ttttttattt atatacgtat
     3601 atatagacta ttatttactt ttaatagatt attaagattt ttattaaaaa aaaattcgtc
     3661 cctcttttta atgcctttta tgcagttttt ttttcccatt cgatatttct atgttcgggt
     3721 ttcagcgtat tttaagttta ataactcgaa aattctgcgt ttcgaaaaaa agcttggcgt
     3781 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     3841 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     3901 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcagatct
     3961 gagctttttc tttccaattt tttttttttc gtcattataa aaatcattac gaccgagatt
     4021 cccggccggg taataactga tataattaaa ttgaagctct aatttgtgag tttagtatac
     4081 atgcatttac ttataataca gttttttagt tttgctggcc gcatcttctc aaatatgctt
     4141 cccagcctgc ttttctgtaa cgttcaccct ctaccttagc atcccttccc tttgcaaata
     4201 gtcctcttcc aacaataata atgtcagatc ctgtagagac cacatcatcc acggttctat
     4261 actgttgacc caatgcgtct cccttgtcat ctaaacccac accgggtgtc ataatcaacc
     4321 aatcgtaacc ttcatctctt ccacccatgt ctctttgagc aataaagccg ataacaaaat
     4381 ctttgtcgct cttcgcaatg tcaacagtac ccttagtata ttctccagta gatagggagc
     4441 ccttgcatga caattctgct aacatcaaaa ggcctctagg ttcctttgtt acttcttctg
     4501 ccgcctgctt caaaccgcta acaatacctg ggcccaccac accgtgtgca ttcgtaatgt
     4561 ctgcccattc tgctattctg tatacacccg cagagtactg caatttgact gtattaccaa
     4621 tgtcagcaaa ttttctgtct tcgaagagta aaaaattgta cttggcggat aatgccttta
     4681 gcggcttaac tgtgccctcc atggaaaaat cagtcaagat atccacatgt gtttttagta
     4741 aacaaatttt gggacctaat gcttcaacta actccagtaa ttccttggtg gtacgaacat
     4801 ccaatgaagc acacaagttt gtttgctttt cgtgcatgat attaaatagc ttggcagcaa
     4861 caggactagg atgagtagca gcacgttcct tatatgtagc tttcgacatg atttatcttc
     4921 gtttccggtt tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc
     4981 aacactacat atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt
     5041 ctgttcggag attaccgaat caaaaaaatt tcaaagaaac cgaaatcaaa aaaaagaata
     5101 aaaaaaaaat gatgaattga attgaaaagc tcagatctgc tgcattaatg aatcggccaa
     5161 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     5221 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagcat cgatggcagc tcgcgttgca
     5281 ttttcggaag cgctcgtttt cggaaacgct ttgaagttcc tattccgaag ttcctattct
     5341 ctagaaagta taggaacttc agagcgcttt tgaaaaccaa aagcgctctg aagacgcact
     5401 ttcaaaaaac caaaaacgca ccggactgta acgagctact aaaatattgc gaataccgct
     5461 tccacaaaca ttgctcaaaa gtatctcttt gctatatatc tctgtgctat atccctatat
     5521 aacctaccca tccacctttc gctccttgaa cttgcatcta aactcgacct ctacattttt
     5581 tatgtttatc tctagtatta ctctttagac aaaaaaattg tagtaagaac tattcataga
     5641 gtgaatcgaa aacaatacga aaatgtaaac atttcctata cgtagtatat agagacaaaa
     5701 tagaagaaac cgttcataat tttctgacca atgaagaatc atcaacgcta tcactttctg
     5761 ttcacaaagt atgcatcgat gctcactcaa aggtcggtaa tacggttatc cacagaatca
     5821 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5881 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5941 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6001 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6061 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6121 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6181 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6241 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6301 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     6361 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6421 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6481 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6541 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     6601 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     6661 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     6721 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     6781 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     6841 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     6901 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     6961 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7021 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7081 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7141 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7201 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7261 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7321 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7381 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7441 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     7501 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     7561 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     7621 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     7681 ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
