LOCUS       62056_2950        8825 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8825)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(115..236)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        481..838
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             889..1821
                     /codon_start=1
                     /label=hRluc
                     /note="Renilla luciferase"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             1873..2661
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    2728..2776
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        2926..3030
                     /label=AmpR promoter
     CDS             3031..3888
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(4049..4637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4753..5036
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"
     CDS             7120..8769
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 catgcaagct gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
       61 cgctgagcaa taactagcat aaccccttgg ggcggccgct tcgagcagac atgataagat
      121 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
      181 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
      241 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gtttttttaa
      301 gcaagtaaaa cctctacaaa tgtggtaaaa tcgaatttta acaaaatatt aacgcttaca
      361 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgcg
      421 gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt
      481 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
      541 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
      601 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      661 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      721 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
      781 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
      841 gcttgattct tctgacacaa cagtctcgaa ccaaaggctg gagccaccat ggcttccaag
      901 gtgtacgacc ccgagcaacg caaacgcatg atcactgggc ctcagtggtg ggctcgctgc
      961 aagcaaatga acgtgctgga ctccttcatc aactactatg attccgagaa gcacgccgag
     1021 aacgccgtga tttttctgca tggtaacgct gcctccagct acctgtggag gcacgtcgtg
     1081 cctcacatcg agcccgtggc tagatgcatc atccctgatc tgatcggaat gggtaagtcc
     1141 ggcaagagcg ggaatggctc atatcgcctc ctggatcact acaagtacct caccgcttgg
     1201 ttcgagctgc tgaaccttcc aaagaaaatc atctttgtgg gccacgactg gggggcttgt
     1261 ctggcctttc actactccta cgagcaccaa gacaagatca aggccatcgt ccatgctgag
     1321 agtgtcgtgg acgtgatcga gtcctgggac gagtggcctg acatcgagga ggatatcgcc
     1381 ctgatcaaga gcgaagaggg cgagaaaatg gtgcttgaga ataacttctt cgtcgagacc
     1441 atgctcccaa gcaagatcat gcggaaactg gagcctgagg agttcgctgc ctacctggag
     1501 ccattcaagg agaagggcga ggttagacgg cctaccctct cctggcctcg cgagatccct
     1561 ctcgttaagg gaggcaagcc cgacgtcgtc cagattgtcc gcaactacaa cgcctacctt
     1621 cgggccagcg acgatctgcc taagatgttc atcgagtccg accctgggtt cttttccaac
     1681 gctattgtcg agggagctaa gaagttccct aacaccgagt tcgtgaaggt gaagggcctc
     1741 cacttcagcc aggaggacgc tccagatgaa atgggtaagt acatcaagag cttcgtggag
     1801 cgcgtgctga agaacgagca gaccggtggt gggagcggag gtggcggatc aggtggcgga
     1861 ggctccggag ggattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     1921 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     1981 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     2041 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     2101 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     2161 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     2221 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     2281 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     2341 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     2401 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     2461 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     2521 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     2581 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     2641 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     2701 cgcccaacct gccatcacga tggccgcaat aaaatatctt tattttcatt acatctgtgt
     2761 gttggttttt tgtgtgaatc gatagcgata atctttgcgc ttgcgttttc ccttgtccag
     2821 atagcccagt agctgacatt catccggggt cagcaccgtt tctgcggact ggctttctac
     2881 gtaatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     2941 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3001 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3061 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3121 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     3181 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttc
     3241 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     3301 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     3361 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     3421 actgcggcca acttacttct gacaactatc ggaggaccga aggagctaac cgcttttttg
     3481 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     3541 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     3601 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     3661 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     3721 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     3781 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     3841 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta attcgaaatg
     3901 accgaccaag cgacgcccaa ccggtatcag ctcactcaaa ggcggtaata cggttatcca
     3961 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4021 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4081 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4141 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4201 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4261 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4321 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4381 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     4441 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     4501 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4561 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4621 gaaaaaaagg atttcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4681 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4741 tccttttata gtccggaaat acaggaacgc acgctggatg gcccttcgct gggatggtga
     4801 aaccatgaaa aatggcagct tcagtggatt aagtgggggt aatgtggcct gtaccctctg
     4861 gttgcatagg tattcatacg gttaaaattt atcaggcgcg attgcggcag tttttcgggt
     4921 ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg ctgaacgcgt tttagcggtg
     4981 cgtacaatta agggattatg gtaaatccac ttactgtctg ccctcgtagc catcgagata
     5041 aaccgcagta ctccggccac gatgcgtccg gcgtagagga tcgagatctc gagctcaagc
     5101 ttcgaattct cttgaatgaa taaagtgtgg aggggataat acccacaaaa tttgggttag
     5161 gaatcacaaa tgatatggca tttgagctgg gctctctctg ttattattaa agaataaggt
     5221 ggagggagat gaattaggaa ttttgcaaag cacaaaattt aatagtattt gctggaatat
     5281 ttagatataa gtggaaagag agttgaagat gtttcatagg tggattagag aaaattttca
     5341 gctaaagaaa acaatgagtt caatttatca acattgtgaa tctgaggtaa aactcaccag
     5401 gtggaaatgg cttgatgacg tttgaaaatg taggaatgga tatggtgtga gatgtcatct
     5461 ggatgtccca gtctggatgc ctttctgctc ctgacaagca aatgtactcc tctcactgtg
     5521 ctccctactc tggttaacag cgcagaagga gtcagaaatc ttggggtcat ttatgatcgc
     5581 tctctctctc catgtatttc cttattgtcc atcttttctt ccttttctcc atcccattgt
     5641 cacttccctg gattttacct ggactatttc aactccctta ctactctccc tgcatccaat
     5701 ctttctctcc tgtaatccag tttgcacttt ccttccagag tgacttttgt gagacaccgg
     5761 aatgttgctt ttctgctatg catctttaaa aggcttccat tgactacaga ataaaacctg
     5821 gactcttgac caagacttta tgatttgatc ctagaattct tgaatctcat ttccagtttt
     5881 ctctccaacc ataccagact acttatcctt aaatgtctcc tgcagctctt cttcctttcc
     5941 accttattga aatctcagta tagagtaaca aagttattct ggctgggcaa aagatattat
     6001 actgtttatt gtggagacaa aaaacaaacc aaaacaagaa gacaagaaaa cgccttgcct
     6061 tcaggttgaa ctcagatgtg gcctcctgaa gctttctcta ggaatattta tagttccact
     6121 ggttatctgc cagctgacag taattcttag ccaaaaattg agttactgtc tcttttatag
     6181 gtaaggttaa gttcactatt tagcacatct gtccttgaga ttgtctagtt acaaacagtt
     6241 tggctacaag taatttgttg tcaagtatct ttctgagtat ctttctgggt tggaagtgtg
     6301 tccacatagc ccttaccttg acctcaataa gatggctact ggggggtgtc attacgtctt
     6361 acgtcatcac gtgtcgggaa ggtggttact aagagaatgt aatgagggcg gggccaagta
     6421 ggacggggcg gggctatgag gagctgggcg gggctggaga ggggtcgggg gctcttctgc
     6481 tattcagccg gtgcgagcag cggcgggagg cagcgcaagg cgaggccact cgacgctagg
     6541 tgccgagagc gtgtccgcga ccaccggctc ggacaagagg cagccgcgta gccaagcgga
     6601 agctggtggt accgggagcg gaggccggag cccaagctcc caggcgggaa gcacccagga
     6661 tcggggaagc cccgggagca gcgcggcggc gcctccctga cccaaggggc cgcagcgacg
     6721 gtcacggggc gcggcgccac agcgcgcgac ccaggccggg attccaaaca ggcagcagag
     6781 gctcctcctc cattcaggcc gcctcacctg cgggcagcgc cgcgccccct ccgctggtat
     6841 agacggcacc tgcgccgcct gggtcaccgg tctccgcccc tcgccccccg gcgccaggcc
     6901 ccggcggcgc acctggacgc gcggagctgc gagacccaag gctcaggccg ggtgcggcgt
     6961 ctttgccacc gcccagggag cgcgcaggtg gagcgggcgc ggctgtgtgc aaccgggccc
     7021 cggccgctgc cgccgtccgg gtcgccgccg ggtctcgaag accgggctgt ctgcgctcgg
     7081 ggaggagccg ccgccagcca ggcaccatgg gatcccacca tggaagatgc caaaaacatt
     7141 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
     7201 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
     7261 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
     7321 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
     7381 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
     7441 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
     7501 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
     7561 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
     7621 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
     7681 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
     7741 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
     7801 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
     7861 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
     7921 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
     7981 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
     8041 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
     8101 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
     8161 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
     8221 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
     8281 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
     8341 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
     8401 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
     8461 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
     8521 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
     8581 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca
     8641 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc
     8701 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag
     8761 atcgccgtgt aattctagtt gtttaaacga gctcgctagc ctcgagtcta gagtcgacct
     8821 gcagg
//
