LOCUS       40924_4145       10468 bp DNA     circular SYN 17-DEC-2018
DEFINITION  PtGAUT12 RNAi binary vector pAGW641, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Biswal AK, Hao Z, Pattathil S, Yang X, Winkeler K, Collins C, 
            Mohanty SS, Richardson EA, Gelineo-Albersheim I, Hunt K, Ryno D, 
            Sykes RW, Turner GB, Ziebell A, Gjersing E, Lukowitz W, Davis MF, 
            Decker SR, Hahn MG, Mohnen D.
  TITLE     Downregulation of GAUT12 in Populus deltoides by RNA silencing 
            results in reduced recalcitrance, increased growth and reduced xylan
            and pectin in a woody biofuel feedstock
  JOURNAL   Biotechnol Biofuels 8, 41 (2015)
  PUBMED    25802552
REFERENCE   2  (bases 1 to 10468)
  AUTHORS   Rottmann WH, Winkeler KA, Biswal AK, Yang X.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2014) ArborGen Inc., 2011 Broadbank Ct., 
            Ridgeville, SC 29472, USA
REFERENCE   3  (bases 1 to 10468)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10468)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Biofuels 8, 41 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-DEC-2014) ArborGen Inc., 2011 Broadbank Ct., Ridgeville, SC 
            29472, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     On Apr 14, 2015 this sequence version replaced KP259612.1.
FEATURES             Location/Qualifiers
     source          1..10468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             802..1593
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             1895..3040
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     misc_feature    3495..3519
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     3857..3873
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(3875..4095)
                     /gene="nos"
                     /label=nopaline synthase (nos) terminator
                     /note="nopaline synthase (nos) terminator"
                     /regulatory_class="terminator"
     terminator      3886..4138
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4144..4935)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(4936..6262)
                     /gene="nos"
                     /note="Arabidopsis thaliana UBIQUITIN10 (At4g05320)
                     promoter plus 5'-UTR"
                     /regulatory_class="promoter"
     terminator      complement(6300..6552)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    6610..6634
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6661..6828
                     /label=fragment of PtGAUT12
                     /note="fragment of PtGAUT12"
     protein_bind    complement(6851..6875)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          complement(6904..8245)
                     /label=chsA intron
                     /note="chalcone synthase A intron from Petunia hybrida"
     protein_bind    8262..8286
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(8309..8476)
                     /label=fragment of PtGAUT12
                     /note="fragment of PtGAUT12"
     protein_bind    complement(8503..8527)
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     rep_origin      10000..10468
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 cgccggcgtt gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac
       61 gttgacactt gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga
      121 tttcggccgg cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac
      181 gcgagtttcc cacagatgat gtggacaagc ctggggataa gtgccctgcg gtattgacac
      241 ttgaggggcg cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt
      301 gctgacagat gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc
      361 agcatttgca agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct
      421 tttaaaccaa tatttataaa ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg
      481 cgcacgccga aggggggtgc ccccccttct cgaaccctcc cggcccgcta acgcgggcct
      541 cccatccccc caggggctgc gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc
      601 gctggcagtc cataattgtg gtttcaaaat cggctccgtc gatactatgt tatacgccaa
      661 ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa
      721 cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag
      781 aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg
      841 atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag
      901 ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc
      961 acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt
     1021 ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc
     1081 gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag
     1141 ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg
     1201 aatagcttag acagccgctt agccgaattg gattacttac tgaataacga tctggccgat
     1261 gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga gctgtatgat
     1321 tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac
     1381 agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc
     1441 agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg
     1501 gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa
     1561 ataaaatatt atattttact ggatgaattg ttttagtacc tagatgtggc gcaacgatgc
     1621 cggcgacaag caggagcgca ccgacttctt ccgcatcaag tgttttggct ctcaggccga
     1681 ggcccacggc aagtatttgg gcaaggggtc gctggtattc gtgcagggca agattcggaa
     1741 taccaagtac gagaaggacg gccagacggt ctacgggacc gacttcattg ccgataaggt
     1801 ggattatctg gacaccaagg caccaggcgg gtcaaatcag gaataagggc acattgcccc
     1861 ggcgtgagtc ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg accggaaggc
     1921 atacaggcaa gaactgatcg acgcggggtt ttccgccgag gatgccgaaa ccatcgcaag
     1981 ccgcaccgtc atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga tggtccagca
     2041 agctacggcc aagatcgagc gcgacagcgt gcaactggct ccccctgccc tgcccgcgcc
     2101 atcggccgcc gtggagcgtt cgcgtcgtct cgaacaggag gcggcaggtt tggcgaagtc
     2161 gatgaccatc gacacgcgag gaactatgac gaccaagaag cgaaaaaccg ccggcgagga
     2221 cctggcaaaa caggtcagcg aggccaagca ggccgcgttg ctgaaacaca cgaagcagca
     2281 gatcaaggaa atgcagcttt ccttgttcga tattgcgccg tggccggaca cgatgcgagc
     2341 gatgccaaac gacacggccc gctctgccct gttcaccacg cgcaacaaga aaatcccgcg
     2401 cgaggcgctg caaaacaagg tcattttcca cgtcaacaag gacgtgaaga tcacctacac
     2461 cggcgtcgag ctgcgggccg acgatgacga actggtgtgg cagcaggtgt tggagtacgc
     2521 gaagcgcacc cctatcggcg agccgatcac cttcacgttc tacgagcttt gccaggacct
     2581 gggctggtcg atcaatggcc ggtattacac gaaggccgag gaatgcctgt cgcgcctaca
     2641 ggcgacggcg atgggcttca cgtccgaccg cgttgggcac ctggaatcgg tgtcgctgct
     2701 gcaccgcttc cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg tcctgatcga
     2761 cgaggaaatc gtcgtgctgt ttgctggcga ccactacacg aaattcatat gggagaagta
     2821 ccgcaagctg tcgccgacgg cccgacggat gttcgactat ttcagctcgc accgggagcc
     2881 gtacccgctc aagctggaaa ccttccgcct catgtgcgga tcggattcca cccgcgtgaa
     2941 gaagtggcgc gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg gcctggtgga
     3001 acacgcctgg gtcaatgatg acctggtgca ttgcaaacgc tagggccttg tggggtcagt
     3061 tccggctggg ggttcagcag ccagcgcttt actggcattt caggaacaag cgggcactgc
     3121 tcgacgcact tgcttcgctc agtatcgctc gggacgcacg gcgcgctcta cgaactgccg
     3181 atagacaact gtcacggtta agcgagaaat gaataagaag gctgataatt cggatctctg
     3241 cgagggagat gatatttgat cacaggcagc aacgctctgt catcgttaca atcaacatgc
     3301 taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat
     3361 agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc
     3421 cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
     3481 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt
     3541 aataacacat tgcggacgtt tttaatgtac tggggtggtt tttcttttca ccagtgagac
     3601 gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac
     3661 gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttccgaaat cggcaaaatc
     3721 ccttataaat caaaagaata gcccgagata gggttgagtg ttgttccagt ttggaacaag
     3781 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     3841 gatggcccac ggccgctcta gaactagtgg atccccccta cgtgcgatct agtaacatag
     3901 atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt
     3961 attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat aacgtcatgc
     4021 attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg ataatcatcg
     4081 caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt gaacgatccc
     4141 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     4201 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     4261 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
     4321 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
     4381 gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
     4441 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     4501 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     4561 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
     4621 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
     4681 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
     4741 cgctgcctcg tcctggagtt cattcagggc accggacagg tcggtcttga caaaaagaac
     4801 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
     4861 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
     4921 atcttgttca atcatctgtt aatcagaaaa actcagatta atcgacaaat tcgatcgcac
     4981 aaactagaaa ctaacaccag atctagatag aaatcacaaa tcgaagagta attattcgac
     5041 aaaactcaaa ttatttgaac aaatcggatg atatttatga aaccctaatc gagaattaag
     5101 atgatatcta acgatcaaac ccagaaaatc gtcttcgatc taagattaac agaatctaaa
     5161 ccaaagaaca tatacgaaat tgggatcgaa cgaaaacaaa atcgaagatt ttgagagaat
     5221 aaggaacaca gaaatttacc ttgatcacgg tagagagaat tgagagaaag tttttaagat
     5281 tttgagaaat tgaaatctga attgtgaaga agaagagctc tttgggtatt gttttataga
     5341 agaagaagaa gaaaagacga ggacgactag gtcacgagaa agctaaggcg gtgaagcaat
     5401 agctaataat aaaatgacac gtgtattgag cgttgtttac acgcaaagtt gtttttggct
     5461 aattgcctta tttttaggtt gaggaaaagt atttgtgctt tgagttgata aacacgactc
     5521 gtgtgtgccg gctgcaacca ctttgacgcc gtttattact gactcgtcga caaccacaat
     5581 ttctaacggt cgtcataaga tccagccgtt gagatttaac gatcgttacg atttatattt
     5641 ttttagcatt atcgttttat tttttaaata tacggtggag ctgaaaattg gcaataattg
     5701 aaccgtgggt cccactgcat tgaagcgtat ttcgtatttt ctagaattct tcgtgcttta
     5761 tttcttttcc tttttgtttt tttttgccat ttatctaatg caagtgggct tataaaatca
     5821 gtgaatttct tggaaaagta acttctttat cgtataacat attgtgaaat tatccatttc
     5881 ttttaatttt ttagtgttat tggatatttt tgtatgatta ttgatttgca taggataatg
     5941 acttttgtat caagttggtg aacaagtctc gttaaaaaag gcaagtggtt tggtgactcg
     6001 atttattctt gttatttaat tcatatatca atggatctta tttggggcct ggtccatatt
     6061 taacactcgt gttcagtcca atgaccaata atattttttc attaataaca atgtaacaag
     6121 aatgatacac aaaacattct ttgaataagt tcgctatgaa gaagggaact tatccggtcc
     6181 tagatcatca gttcatacaa acctccatag agttcaacat cttaaacaag gatatcctga
     6241 tccgttgacg gcgcgccaag cggccgcatt taaatgggcc ctcgcgatgg gaaattcccg
     6301 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
     6361 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
     6421 aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta
     6481 tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat
     6541 gtttgaacga tcggggaaat tcgagctcgc tagatgcatg ctcgagcggc cgccagtgtg
     6601 atggatatca caagtttgta caaaaaagca ggctccgcgg ccgccccctt cacccccggg
     6661 gagagttctt cacaaatcca aggccttgaa tccagaaagt aaaatattca taaccaggca
     6721 tttgtctgct tggtttttta gaattcatcc tctgtgggtg aacaaccctt ccctattact
     6781 tcaaatcttg attcaatttt aatgagaaat taagcatgtt atggattttc tagaaagggt
     6841 gggcgcgccg acccagcttt cttgtacaaa gtggtgatat ctgcagaatt cgccctccta
     6901 ggcattgacc aaaaaagatg tgaagaaaac actaaaacat aaactccaag gcaagtgtaa
     6961 gtaagtgttt ataggtgctt taaagtactc aaattgaatg catagcatgc aaaaaccctc
     7021 aataaataaa agaagaaaat taagagcttt cattttcaca tgtcagaaac attctgatgt
     7081 acatttagtt ccacaaagtt agtaagattc atgatctttt gagctttcat gtcagtttct
     7141 ctttctttgg agtgagaaaa aacattttaa aaaaaaataa gggattaaag atttctaatt
     7201 tcaaagcatt tggacaacta atttctaaaa ttttctaacc tcagtgttaa gcaatgtatc
     7261 aattaacatg tgttgtattt tactagttaa ggaatggtaa agattggatt tagtcaatat
     7321 cataaaaaac atctcacgtg tgatgctact taaggaactt gctcataatt cattgtaaga
     7381 tgaaaaatag aaagaaaagg acaaaaaatt actaaaaggt taaagataac atcaagtact
     7441 agctagaaac acacgaattt tcgacagaaa tcttggaatt ttgctagctc gctgggaaac
     7501 atctgtctgt gtttataacc ttcgaaaaat ctgttttatt aatgtaccaa atcaaactaa
     7561 aaaacaaatt aacggagaga gtatagattt ggattagttt atgactccat cttattccct
     7621 ccgtttcact ttatgtgtta tagtttnact cgccacggag attaagaaat aaatgaagac
     7681 ttttaaaatt tgtagtctaa agtgagacat agaacccgtt tgggtgagct taaaagtggc
     7741 ttataagctt ataaacccat taatatatgt ttggtaactt aaaaattttt ggacttataa
     7801 gtcataagtg gcttaaaagc tactttttga gaagttagga ttttctaact tctcaaaaan
     7861 tgtcactttt tgacttataa gccacttaaa ataagctgct tataagccca cccaaacagg
     7921 ctcatagata cttttgtggt tgtaaatcat tttattaatg gcaaaattgt aatttcaaaa
     7981 ttaaattgtt tataatgctg acattttctt ttggaaccga ctaaaaagga aatattgtca
     8041 cataaattga aacggaaaca gtaattacta atggagtata acaaaagtcc tcccctcttt
     8101 ctaccttccc acaattcgtc ggccacccca acccaaaaag agtgtgacaa aaaaaaaaac
     8161 aagaaataat tgtcaaattt caatcaaatg aagagccaat taagataaaa cgttgaatgt
     8221 aataatgtaa cataagaaat tcttctagca cgcgtagtat caccactttg tacaagaaag
     8281 ctgggtcggc gcgcccaccc tttctagaaa atccataaca tgcttaattt ctcattaaaa
     8341 ttgaatcaag atttgaagta atagggaagg gttgttcacc cacagaggat gaattctaaa
     8401 aaaccaagca gacaaatgcc tggttatgaa tattttactt tctggattca aggccttgga
     8461 tttgtgaaga actctccccg ggggtgaagg gggcggccgc ggagcctgct tttttgtaca
     8521 aacttgtgat actcctaggc tcgagcccgg ggatctgcat ctgaaataaa acaatagaac
     8581 aagtagaaac caatcagcga acatatacca aatcaaaagc cgtaagagaa atcaaaacaa
     8641 caccaaagag aaacggatct aaacataaga aacctaaaac agagagaatc gaacaaagaa
     8701 aacacaaaaa ttgaatagat cgtccttgaa aatcctaatt tcacaatcaa gcaagaaatt
     8761 acacagatgt aaacactacg aatcgatatc ttagtaatca ggacaaaatt tagaagctgg
     8821 attgacgaaa cgaacaatat tgtcaaaagc aatttataca aaagattcaa taatccacat
     8881 aacaaaaatt ggagatcaga tacgaatcaa aaacaaaaag aatcagaaaa tataccttga
     8941 aagagagagt cgcgagagat ttgcagagat cgctttaggc tttgggagag attgaagagt
     9001 cagaaaaaga cgaaaggatg aattattatc ttccacacga aggtcttctt tatatcgcaa
     9061 accaaaagcc caaaaccgtc ttttctatta atgagaataa aatatcttta gccaaaacaa
     9121 aaaaaggaag atatcagttg aggattatta tcacgaaact aaaggaagga atcatatgat
     9181 acgtgtctat tttccaccgt gcgtttttaa aagaccgact caagtagaaa catcctatgg
     9241 tggtggttgg attaggtcat ccattacatc tgcttcactg acatttttct atttttcttt
     9301 ttgtatatac ttttcctcaa ataatttctt tcttttctat agaagaattt aatcaataag
     9361 gaaaaagttc aaaaaagatt ctttccatta agactatgtc ttggttaacc caacccatta
     9421 agaataagca atcataatat atatagagaa tactaatact atatatgaga tttttctttt
     9481 aatttcatgt tgattatgat agtttatctt cttgatttaa tttatcaata cttggcataa
     9541 aagattctaa tctactctaa taaagaaaag aaaaaaaagt atctaccatt gactaattaa
     9601 aataaggaaa cttatctacc aaatttgagt attttttaga acaatctttt tggtttaatt
     9661 ccaaaactct aaacctaatt gttgggaaaa aggacctaat ttttaagaaa agttaataat
     9721 tagaagatct gtatgttttt ttttttgatc caagttttta tttcttttct ctttttttca
     9781 tgataaaatc tatgtttttt tagtctacaa ttaaagtaat tgttattatt ttctttatct
     9841 ttttttgttg ttgttgttaa ttcccttttt ttttttttaa cagcaacttc ttaaaaaaaa
     9901 aaacagttgg gccttgaatt tatttcaggc ctgcgttatt aagcccagat aataactcaa
     9961 aacaaaaaaa atgttgaacc ggaataaacc cgcgagatta aatgccggtt ttcaggtaac
    10021 atagaagaag aatatatgag gattgaagaa gtattcaaga ggcggaacaa ttcacaagtc
    10081 caagagctta aatttctcct cactcttctg ctacagactc ggaactcttt ctctttgcta
    10141 aaataagatg ttcaggattt ttgttgcccg acaattcatg tatctcacac tctctctctt
    10201 ctctgttctt actactctgt tacattacca ccaactcaag actttcttcc acaatggcgt
    10261 ttatgagact tggctccaaa tccgaagctg ggctcctgca ggtaccttaa ttaaaagttt
    10321 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
    10381 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
    10441 gcatgccaac cacagggttc cccagatc
//
