LOCUS       40924_4140       10326 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pAGW633, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10326)
  AUTHORS   Biswal AK, Atmodjo MA, Li M, Yoo CG, Pu Y, Lee Y-C., Zhang J-Y., 
            Ramanna H, Bray AL, King ZR, LaFayette PR, Mohanty SS, Ryno D, Yee 
            K, Thompson OA, Rodriguez M Jr., Winkeler KA, Collins C, Yang X, Tan
            L, Sykes RW, Gjersing EL, Ziebell A, Turner GB, Decker SR, Parrott 
            WA, Udvardi MK, Mielenz J, Davis MF, Nelson RS, Ragauskas AJ, Mohnen
            D.
  TITLE     Targeted modification of pectin biosynthesis to improve growth and 
            deconstruction of woody and grass bioenergy feedstocks
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10326)
  AUTHORS   Rottmann WH, Winkeler KA, Biswal AK, Yang X.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2016) Biochemistry and Molecular Biology, Complex 
            Carbohydrate Research Center, The University of Georgia, 315 
            Riverbend Rd, Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 10326)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10326)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-AUG-2016) Biochemistry and Molecular Biology, Complex 
            Carbohydrate Research Center, The University of Georgia, 315 
            Riverbend Rd, Athens, GA 30602, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             801..1592
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             1894..3039
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     misc_feature    3399..3423
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     3761..3777
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(3779..3999)
                     /gene="nos"
                     /label=nopaline synthase (nos) terminator
                     /note="nopaline synthase (nos) terminator"
                     /regulatory_class="terminator"
     terminator      3790..4042
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4048..4839)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    complement(4840..6166)
                     /gene="nos"
                     /note="Arabidopsis thaliana ubiquitni 10 (At4g05320)
                     promoter and 5' UTR"
     terminator      complement(6204..6456)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    6514..6538
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(6565..6714)
                     /label=fragment of PtGAUT4
                     /note="fragment of PtGAUT4"
     protein_bind    complement(6732..6756)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          complement(6785..8126)
                     /label=chsA intron
                     /note="chalcone synthase A intron from Petunia hybrida"
     protein_bind    8143..8167
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(8185..8334)
                     /label=fragment of PtGAUT4
                     /note="fragment of PtGAUT4"
     protein_bind    complement(8361..8385)
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     rep_origin      10000..10326
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga ggggcggacg
       61 ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg caggctcgat
      121 ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg
      181 cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg tattgacact
      241 tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag gggcagagtg
      301 ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc agaaaatcca
      361 gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt taacctgctt
      421 ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc gcgtgaccgc
      481 gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa cgcgggcctc
      541 ccatcccccc aggggctgcg cccctcggcc gcgaacggcc tcaccccaaa aatggcagcg
      601 ctggcagtcc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac
      661 tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac
      721 agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga
      781 aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga
      841 tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc
      901 tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca
      961 cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc
     1021 caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg
     1081 atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc
     1141 tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga
     1201 atagcttaga cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg
     1261 tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt
     1321 ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca
     1381 gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca
     1441 gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg
     1501 aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa
     1561 taaaatatta tattttactg gatgaattgt tttagtacct agatgtggcg caacgatgcc
     1621 ggcgacaagc aggagcgcac cgacttcttc cgcatcaagt gttttggctc tcaggccgag
     1681 gcccacggca agtatttggg caaggggtcg ctggtattcg tgcagggcaa gattcggaat
     1741 accaagtacg agaaggacgg ccagacggtc tacgggaccg acttcattgc cgataaggtg
     1801 gattatctgg acaccaaggc accaggcggg tcaaatcagg aataagggca cattgccccg
     1861 gcgtgagtcg gggcaatccc gcaaggaggg tgaatgaatc ggacgtttga ccggaaggca
     1921 tacaggcaag aactgatcga cgcggggttt tccgccgagg atgccgaaac catcgcaagc
     1981 cgcaccgtca tgcgtgcgcc ccgcgaaacc ttccagtccg tcggctcgat ggtccagcaa
     2041 gctacggcca agatcgagcg cgacagcgtg caactggctc cccctgccct gcccgcgcca
     2101 tcggccgccg tggagcgttc gcgtcgtctc gaacaggagg cggcaggttt ggcgaagtcg
     2161 atgaccatcg acacgcgagg aactatgacg accaagaagc gaaaaaccgc cggcgaggac
     2221 ctggcaaaac aggtcagcga ggccaagcag gccgcgttgc tgaaacacac gaagcagcag
     2281 atcaaggaaa tgcagctttc cttgttcgat attgcgccgt ggccggacac gatgcgagcg
     2341 atgccaaacg acacggcccg ctctgccctg ttcaccacgc gcaacaagaa aatcccgcgc
     2401 gaggcgctgc aaaacaaggt cattttccac gtcaacaagg acgtgaagat cacctacacc
     2461 ggcgtcgagc tgcgggccga cgatgacgaa ctggtgtggc agcaggtgtt ggagtacgcg
     2521 aagcgcaccc ctatcggcga gccgatcacc ttcacgttct acgagctttg ccaggacctg
     2581 ggctggtcga tcaatggccg gtattacacg aaggccgagg aatgcctgtc gcgcctacag
     2641 gcgacggcga tgggcttcac gtccgaccgc gttgggcacc tggaatcggt gtcgctgctg
     2701 caccgcttcc gcgtcctgga ccgtggcaag aaaacgtccc gttgccaggt cctgatcgac
     2761 gaggaaatcg tcgtgctgtt tgctggcgac cactacacga aattcatatg ggagaagtac
     2821 cgcaagctgt cgccgacggc ccgacggatg ttcgactatt tcagctcgca ccgggagccg
     2881 tacccgctca agctggaaac cttccgcctc atgtgcggat cggattccac ccgcgtgaag
     2941 aagtggcgcg agcaggtcgg cgaagcctgc gaagagttgc gaggcagcgg cctggtggaa
     3001 cacgcctggg tcaatgatga cctggtgcat tgcaaacgct agggccttgt ggggtcagtt
     3061 ccggctgggg gttcagcagc cagcgcgaca gcctgtcacg gttaagcgag aaatgaataa
     3121 gaaggctgat aattcggatc tctgcgaggg agatgatatt tgatcacagg cagcaacgct
     3181 ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc
     3241 agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca
     3301 acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt
     3361 gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta
     3421 aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat gtactggggt
     3481 ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg
     3541 agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat
     3601 ggtggttccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga gatagggttg
     3661 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     3721 gggcgaaaaa ccgtctatca gggcgatggc ccacggccgc tctagaacta gtggatcccc
     3781 cctacgtgcg atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc
     3841 gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa
     3901 cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca
     3961 acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt
     4021 attgccaaat gtttgaacga tccctcagaa gaactcgtca agaaggcgat agaaggcgat
     4081 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
     4141 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
     4201 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     4261 caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag
     4321 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
     4381 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     4441 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     4501 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     4561 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     4621 cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgg agttcattca gggcaccgga
     4681 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     4741 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     4801 ggccggagaa cctgcgtgca atccatcttg ttcaatcatc tgttaatcag aaaaactcag
     4861 attaatcgac aaattcgatc gcacaaacta gaaactaaca ccagatctag atagaaatca
     4921 caaatcgaag agtaattatt cgacaaaact caaattattt gaacaaatcg gatgatattt
     4981 atgaaaccct aatcgagaat taagatgata tctaacgatc aaacccagaa aatcgtcttc
     5041 gatctaagat taacagaatc taaaccaaag aacatatacg aaattgggat cgaacgaaaa
     5101 caaaatcgaa gattttgaga gaataaggaa cacagaaatt taccttgatc acggtagaga
     5161 gaattgagag aaagttttta agattttgag aaattgaaat ctgaattgtg aagaagaaga
     5221 gctctttggg tattgtttta tagaagaaga agaagaaaag acgaggacga ctaggtcacg
     5281 agaaagctaa ggcggtgaag caatagctaa taataaaatg acacgtgtat tgagcgttgt
     5341 ttacacgcaa agttgttttt ggctaattgc cttattttta ggttgaggaa aagtatttgt
     5401 gctttgagtt gataaacacg actcgtgtgt gccggctgca accactttga cgccgtttat
     5461 tactgactcg tcgacaacca caatttctaa cggtcgtcat aagatccagc cgttgagatt
     5521 taacgatcgt tacgatttat atttttttag cattatcgtt ttatttttta aatatacggt
     5581 ggagctgaaa attggcaata attgaaccgt gggtcccact gcattgaagc gtatttcgta
     5641 ttttctagaa ttcttcgtgc tttatttctt ttcctttttg tttttttttg ccatttatct
     5701 aatgcaagtg ggcttataaa atcagtgaat ttcttggaaa agtaacttct ttatcgtata
     5761 acatattgtg aaattatcca tttcttttaa ttttttagtg ttattggata tttttgtatg
     5821 attattgatt tgcataggat aatgactttt gtatcaagtt ggtgaacaag tctcgttaaa
     5881 aaaggcaagt ggtttggtga ctcgatttat tcttgttatt taattcatat atcaatggat
     5941 cttatttggg gcctggtcca tatttaacac tcgtgttcag tccaatgacc aataatattt
     6001 tttcattaat aacaatgtaa caagaatgat acacaaaaca ttctttgaat aagttcgcta
     6061 tgaagaaggg aacttatccg gtcctagatc atcagttcat acaaacctcc atagagttca
     6121 acatcttaaa caaggatatc ctgatccgtt gacggcgcgc caagcggccg catttaaatg
     6181 ggccctcgcg atgggaaatt cccgatctag taacatagat gacaccgcgc gcgataattt
     6241 atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac
     6301 tctaatcata aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg
     6361 cttaacgtaa ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa
     6421 tcttaagaaa ctttattgcc aaatgtttga acgatcgggg aaattcgagc tcgctagatg
     6481 catgctcgag cggccgccag tgtgatggat atcacaagtt tgtacaaaaa agcaggctcc
     6541 gcggccgccc ccttcacccc cgggataggc atacccaagt atcgaagcaa ctgggcaaaa
     6601 tatgttgatt atgatcatgc atatttgcga gagtgcaata tcaatccata aaatatagtc
     6661 ctcgtggtgt catccattca ttagagctcc atgcaaatgc tgtgccaaca tttttaaggg
     6721 tgggcgcgcc gacccagctt tcttgtacaa agtggtgata tctgcagaat tcgccctcct
     6781 aggcattgac caaaaaagat gtgaagaaaa cactaaaaca taaactccaa ggcaagtgta
     6841 agtaagtgtt tataggtgct ttaaagtact caaattgaat gcatagcatg caaaaaccct
     6901 caataaataa aagaagaaaa ttaagagctt tcattttcac atgtcagaaa cattctgatg
     6961 tacatttagt tccacaaagt tagtaagatt catgatcttt tgagctttca tgtcagtttc
     7021 tctttctttg gagtgagaaa aaacatttta aaaaaaaata agggattaaa gatttctaat
     7081 ttcaaagcat ttggacaact aatttctaaa attttctaac ctcagtgtta agcaatgtat
     7141 caattaacat gtgttgtatt ttactagtta aggaatggta aagattggat ttagtcaata
     7201 tcataaaaaa catctcacgt gtgatgctac ttaaggaact tgctcataat tcattgtaag
     7261 atgaaaaata gaaagaaaag gacaaaaaat tactaaaagg ttaaagataa catcaagtac
     7321 tagctagaaa cacacgaatt ttcgacagaa atcttggaat tttgctagct cgctgggaaa
     7381 catctgtctg tgtttataac cttcgaaaaa tctgttttat taatgtacca aatcaaacta
     7441 aaaaacaaat taacggagag agtatagatt tggattagtt tatgactcca tcttattccc
     7501 tccgtttcac tttatgtgtt atagtttnac tcgccacgga gattaagaaa taaatgaaga
     7561 cttttaaaat ttgtagtcta aagtgagaca tagaacccgt ttgggtgagc ttaaaagtgg
     7621 cttataagct tataaaccca ttaatatatg tttggtaact taaaaatttt tggacttata
     7681 agtcataagt ggcttaaaag ctactttttg agaagttagg attttctaac ttctcaaaaa
     7741 ntgtcacttt ttgacttata agccacttaa aataagctgc ttataagccc acccaaacag
     7801 gctcatagat acttttgtgg ttgtaaatca ttttattaat ggcaaaattg taatttcaaa
     7861 attaaattgt ttataatgct gacattttct tttggaaccg actaaaaagg aaatattgtc
     7921 acataaattg aaacggaaac agtaattact aatggagtat aacaaaagtc ctcccctctt
     7981 tctaccttcc cacaattcgt cggccacccc aacccaaaaa gagtgtgaca aaaaaaaaaa
     8041 caagaaataa ttgtcaaatt tcaatcaaat gaagagccaa ttaagataaa acgttgaatg
     8101 taataatgta acataagaaa ttcttctagc acgcgtagta tcaccacttt gtacaagaaa
     8161 gctgggtcgg cgcgcccacc cttaaaaatg ttggcacagc atttgcatgg agctctaatg
     8221 aatggatgac accacgagga ctatatttta tggattgata ttgcactctc gcaaatatgc
     8281 atgatcataa tcaacatatt ttgcccagtt gcttcgatac ttgggtatgc ctatcccggg
     8341 ggtgaagggg gcggccgcgg agcctgcttt tttgtacaaa cttgtgatac tcctaggctc
     8401 gagcccgggg atctgcatct gaaataaaac aatagaacaa gtagaaacca atcagcgaac
     8461 atataccaaa tcaaaagccg taagagaaat caaaacaaca ccaaagagaa acggatctaa
     8521 acataagaaa cctaaaacag agagaatcga acaaagaaaa cacaaaaatt gaatagatcg
     8581 tccttgaaaa tcctaatttc acaatcaagc aagaaattac acagatgtaa acactacgaa
     8641 tcgatatctt agtaatcagg acaaaattta gaagctggat tgacgaaacg aacaatattg
     8701 tcaaaagcaa tttatacaaa agattcaata atccacataa caaaaattgg agatcagata
     8761 cgaatcaaaa acaaaaagaa tcagaaaata taccttgaaa gagagagtcg cgagagattt
     8821 gcagagatcg ctttaggctt tgggagagat tgaagagtca gaaaaagacg aaaggatgaa
     8881 ttattatctt ccacacgaag gtcttcttta tatcgcaaac caaaagccca aaaccgtctt
     8941 ttctattaat gagaataaaa tatctttagc caaaacaaaa aaaggaagat atcagttgag
     9001 gattattatc acgaaactaa aggaaggaat catatgatac gtgtctattt tccaccgtgc
     9061 gtttttaaaa gaccgactca agtagaaaca tcctatggtg gtggttggat taggtcatcc
     9121 attacatctg cttcactgac atttttctat ttttcttttt gtatatactt ttcctcaaat
     9181 aatttctttc ttttctatag aagaatttaa tcaataagga aaaagttcaa aaaagattct
     9241 ttccattaag actatgtctt ggttaaccca acccattaag aataagcaat cataatatat
     9301 atagagaata ctaatactat atatgagatt tttcttttaa tttcatgttg attatgatag
     9361 tttatcttct tgatttaatt tatcaatact tggcataaaa gattctaatc tactctaata
     9421 aagaaaagaa aaaaaagtat ctaccattga ctaattaaaa taaggaaact tatctaccaa
     9481 atttgagtat tttttagaac aatctttttg gtttaattcc aaaactctaa acctaattgt
     9541 tgggaaaaag gacctaattt ttaagaaaag ttaataatta gaagatctgt atgttttttt
     9601 ttttgatcca agtttttatt tcttttctct ttttttcatg ataaaatcta tgttttttta
     9661 gtctacaatt aaagtaattg ttattatttt ctttatcttt ttttgttgtt gttgttaatt
     9721 cccttttttt ttttttaaca gcaacttctt aaaaaaaaaa acagttgggc cttgaattta
     9781 tttcaggcct gcgttattaa gcccagataa taactcaaaa caaaaaaaat gttgaaccgg
     9841 aataaacccg cgagattaaa tgccggtttt caggtaacat agaagaagaa tatatgagga
     9901 ttgaagaagt attcaagagg cggaacaatt cacaagtcca agagcttaaa tttctcctca
     9961 ctcttctgct acagactcgg aactctttct ctttgctaaa ataagatgtt caggattttt
    10021 gttgcccgac aattcatgta tctcacactc tctctcttct ctgttcttac tactctgtta
    10081 cattaccacc aactcaagac tttcttccac aatggcgttt atgagacttg gctccaaatc
    10141 cgaagctggg ctcctgcagg taccttaatt aaaagtttaa actatcagtg tttgacagga
    10201 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa
    10261 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc
    10321 cagatc
//
