LOCUS       40924_4135        9773 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pAGW570, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Biswal AK, Atmodjo MA, Pattathil S, Amos RA, Yang X, Winkeler K, 
            Collins C, Mohanty SS, Ryno D, Tan L, Gelineo-Albersheim I, Hunt K, 
            Sykes RW, Turner GB, Ziebell A, Davis MF, Decker SR, Hahn MG, Mohnen
            D.
  TITLE     Increased xylan production by overexpression of 
            GalactUronosylTransferase12 (GAUT12) results in increased 
            recalcitrance and decreased growth in Populus woody biofuel 
            feedstock
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9773)
  AUTHORS   Rottmann W, Winkeler K, Biswal AK, Yang X.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2017) Biochemistry and Molecular Biology; Complex 
            Carbohydrate Research Center, The University of Georgia, 315 
            Riverbend Rd, Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 9773)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9773)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-JUN-2017) Biochemistry and Molecular Biology; Complex 
            Carbohydrate Research Center, The University of Georgia, 315 
            Riverbend Rd, Athens, GA 30602, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      104..634
                     /label=oriV
                     /note="incP origin of replication"
     CDS             907..1698
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             2000..3145
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     misc_feature    3505..3529
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     3867..3883
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(3885..4105)
                     /label=nopaline synthase (nos) terminator
                     /note="nopaline synthase (nos) terminator"
                     /regulatory_class="terminator"
     terminator      3896..4148
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4154..4945)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(4946..6272)
                     /note="Arabidopsis thaliana UBIQUITIN10 (At4g05320)
                     promoter plus 5'-UTR"
                     /regulatory_class="promoter"
     terminator      complement(6310..6562)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    6597..6621
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(6646..8247)
                     /codon_start=1
                     /product="galacturonosyltransferase 12"
                     /label=galacturonosyltransferase 12
                     /note="PtGAUT12.1 (Potri.001G416800); GAUT12"
                     /protein_id="AUG68175.1"
                     /translation="MQLHISPSLRHVTVFPGKGVREFIKVRVGARRVSYRMLFYSLLFF
                     TFLLRFVFVLSTVDSIDGETKCSTLGCLGKRLGPRILGRRLDSAVPEVMFQVLEQPLGN
                     DELKGRSDIPQTLEEFMDEVKNTRLDAKTFALKLREMVTLLEQRTRNAKIQEYLYRHVA
                     SSSIPKQLHCLALRLASEHSTNAAARLQLPLPELVPALVDNTYFHFVLASDNVLAAAVV
                     ANSLVQNALRPQKFVLHIITDRKTYSPMQAWFSLHPLAPAIIEVKALHHFDWFAKGKVP
                     VMEAMEKDQRVRSQFRGGSSAIVANNTEKPHIIAAKLQTLSPKYNSVMNHIRIHLPELF
                     PSLNKVVFLDDDIVVQSDLSPLWDIDMNGKVNGAVETCRGEDKFVMSKKLKSYLNFSHP
                     LISENFKPNECAWAYGMNIFDLEAWRKTNISTTYHHWVEENLKSDLSLWQLGTLPPGLI
                     AFHGHVHVIDPFWHMLGLGYQENTSLADAETAGVIHFNGRAKPWLDIAFPQLRPLWAKY
                     INFSDKFIKGCHIRPS"
     protein_bind    complement(8274..8298)
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      complement(8320..9672)
                     /note="Arabidopsis thaliana UBIQUITIN3 (At5g03240) promoter
                     (- intron)"
                     /regulatory_class="promoter"
     primer_bind     complement(9676..9692)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    9745..9769
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
       61 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc agatccgccg gcgttgtgga
      121 tacctcgcgg aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg
      181 gccgactcac ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg
      241 tggagctggc cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag
      301 atgatgtgga caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact
      361 actgacagat gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg
      421 gcgcacctat tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt
      481 ttccgcccgt ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt
      541 ataaaccttg tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg
      601 ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg
      661 gctgcgcccc tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccataa
      721 ttgtggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt
      781 tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt
      841 cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa
      901 taataaatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc
      961 tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat
     1021 gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa
     1081 cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac
     1141 tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc
     1201 tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc
     1261 atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc
     1321 cgcttagccg aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac
     1381 tgggaagaag acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa
     1441 aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg
     1501 aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg
     1561 tatgacattg ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc
     1621 gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt
     1681 ttactggatg aattgtttta gtacctagat gtggcgcaac gatgccggcg acaagcagga
     1741 gcgcaccgac ttcttccgca tcaagtgttt tggctctcag gccgaggccc acggcaagta
     1801 tttgggcaag gggtcgctgg tattcgtgca gggcaagatt cggaatacca agtacgagaa
     1861 ggacggccag acggtctacg ggaccgactt cattgccgat aaggtggatt atctggacac
     1921 caaggcacca ggcgggtcaa atcaggaata agggcacatt gccccggcgt gagtcggggc
     1981 aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg aaggcataca ggcaagaact
     2041 gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc gcaagccgca ccgtcatgcg
     2101 tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc cagcaagcta cggccaagat
     2161 cgagcgcgac agcgtgcaac tggctccccc tgccctgccc gcgccatcgg ccgccgtgga
     2221 gcgttcgcgt cgtctcgaac aggaggcggc aggtttggcg aagtcgatga ccatcgacac
     2281 gcgaggaact atgacgacca agaagcgaaa aaccgccggc gaggacctgg caaaacaggt
     2341 cagcgaggcc aagcaggccg cgttgctgaa acacacgaag cagcagatca aggaaatgca
     2401 gctttccttg ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc caaacgacac
     2461 ggcccgctct gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg cgctgcaaaa
     2521 caaggtcatt ttccacgtca acaaggacgt gaagatcacc tacaccggcg tcgagctgcg
     2581 ggccgacgat gacgaactgg tgtggcagca ggtgttggag tacgcgaagc gcacccctat
     2641 cggcgagccg atcaccttca cgttctacga gctttgccag gacctgggct ggtcgatcaa
     2701 tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga cggcgatggg
     2761 cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc gcttccgcgt
     2821 cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg aaatcgtcgt
     2881 gctgtttgct ggcgaccact acacgaaatt catatgggag aagtaccgca agctgtcgcc
     2941 gacggcccga cggatgttcg actatttcag ctcgcaccgg gagccgtacc cgctcaagct
     3001 ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt ggcgcgagca
     3061 ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg gtggaacacg cctgggtcaa
     3121 tgatgacctg gtgcattgca aacgctaggg ccttgtgggg tcagttccgg ctgggggttc
     3181 agcagccagc gcgacagcct gtcacggtta agcgagaaat gaataagaag gctgataatt
     3241 cggatctctg cgagggagat gatatttgat cacaggcagc aacgctctgt catcgttaca
     3301 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
     3361 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
     3421 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
     3481 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
     3541 tagacaactt aataacacat tgcggacgtt tttaatgtac tggggtggtt tttcttttca
     3601 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
     3661 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttccgaaat
     3721 cggcaaaatc ccttataaat caaaagaata gcccgagata gggttgagtg ttgttccagt
     3781 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     3841 ctatcagggc gatggcccac ggccgctcta gaactagtgg atccccccta cgtgcgatct
     3901 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
     3961 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
     4021 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
     4081 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
     4141 gaacgatccc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg
     4201 gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag
     4261 caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac
     4321 agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc
     4381 catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt
     4441 cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt
     4501 ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag
     4561 ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag
     4621 gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc
     4681 ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc
     4741 acgatagccg cgctgcctcg tcctggagtt cattcagggc accggacagg tcggtcttga
     4801 caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga
     4861 ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg
     4921 cgtgcaatcc atcttgttca atcatctgtt aatcagaaaa actcagatta atcgacaaat
     4981 tcgatcgcac aaactagaaa ctaacaccag atctagatag aaatcacaaa tcgaagagta
     5041 attattcgac aaaactcaaa ttatttgaac aaatcggatg atatttatga aaccctaatc
     5101 gagaattaag atgatatcta acgatcaaac ccagaaaatc gtcttcgatc taagattaac
     5161 agaatctaaa ccaaagaaca tatacgaaat tgggatcgaa cgaaaacaaa atcgaagatt
     5221 ttgagagaat aaggaacaca gaaatttacc ttgatcacgg tagagagaat tgagagaaag
     5281 tttttaagat tttgagaaat tgaaatctga attgtgaaga agaagagctc tttgggtatt
     5341 gttttataga agaagaagaa gaaaagacga ggacgactag gtcacgagaa agctaaggcg
     5401 gtgaagcaat agctaataat aaaatgacac gtgtattgag cgttgtttac acgcaaagtt
     5461 gtttttggct aattgcctta tttttaggtt gaggaaaagt atttgtgctt tgagttgata
     5521 aacacgactc gtgtgtgccg gctgcaacca ctttgacgcc gtttattact gactcgtcga
     5581 caaccacaat ttctaacggt cgtcataaga tccagccgtt gagatttaac gatcgttacg
     5641 atttatattt ttttagcatt atcgttttat tttttaaata tacggtggag ctgaaaattg
     5701 gcaataattg aaccgtgggt cccactgcat tgaagcgtat ttcgtatttt ctagaattct
     5761 tcgtgcttta tttcttttcc tttttgtttt tttttgccat ttatctaatg caagtgggct
     5821 tataaaatca gtgaatttct tggaaaagta acttctttat cgtataacat attgtgaaat
     5881 tatccatttc ttttaatttt ttagtgttat tggatatttt tgtatgatta ttgatttgca
     5941 taggataatg acttttgtat caagttggtg aacaagtctc gttaaaaaag gcaagtggtt
     6001 tggtgactcg atttattctt gttatttaat tcatatatca atggatctta tttggggcct
     6061 ggtccatatt taacactcgt gttcagtcca atgaccaata atattttttc attaataaca
     6121 atgtaacaag aatgatacac aaaacattct ttgaataagt tcgctatgaa gaagggaact
     6181 tatccggtcc tagatcatca gttcatacaa acctccatag agttcaacat cttaaacaag
     6241 gatatcctga tccgttgacg gcgcgccaag cggccgcatt taaatgggcc ctcgcgatgg
     6301 gaaattcccg atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc
     6361 gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa
     6421 cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca
     6481 acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt
     6541 attgccaaat gtttgaacga tcggggaaat tcgagctcgc tagcacgcgt agtatcacca
     6601 ctttgtacaa gaaagctggg tcggcgcgcc cacccttacg cgtagttaag atggcctaat
     6661 atgacagccc tttatgaact tgtcagaaaa gttgatgtac ttagcccaca gggggcggag
     6721 ctgagggaat gctatatcta gccaaggctt tgctcgacca ttgaaatgga tgacgccggc
     6781 agtctcagca tctgccaaac ttgtattttc ttggtagcct agacccaaca tgtgccaaaa
     6841 gggatcgata acgtggacgt ggccatggaa tgctatcaga cctggaggta gtgttcctag
     6901 ctgccacaag ctcaggtctg atttcaagtt ctcttcaacc cagtgatggt atgttgtgct
     6961 tatgttggtt ttcctccaag cctctaaatc aaatatgttc atgccataag cccaggcaca
     7021 ttcgttcggc ttgaaatttt ctgatatcaa gggatgagag aagttcaaat agctcttcaa
     7081 cttctttgac atgacaaact tgtcttctcc tcgacaggtt tctactgccc cgttaacctt
     7141 tccattcatg tcaatgtccc agagaggcga aagatcggac tgcaccacta tgtcatcgtc
     7201 taggaacaca accttgttta ggcttgggaa caactcaggt agatgaattc ggatgtgatt
     7261 catcactgaa ttgtatttgg gactaagtgt ttgtagtttt gctgcgataa tatgaggctt
     7321 ctcggtgtta tttgctacaa ttgcagatga accccctcta aactgtgacc tcaccctctg
     7381 atccttttcc attgcttcca tgactggcac tttccccttt gcgaaccaat cgaaatgatg
     7441 caatgccttg acctcaatta ttgccggagc caaagggtgc agtgagaacc atgcttgcat
     7501 aggtgaataa gttttcctat ctgttattat gtgcagcaca aacttctggg ggcgcaacgc
     7561 attttggaca agtgaattgg caacaacagc agcagcaagc acattgtctg aagccaggac
     7621 aaagtgaaaa taagtattgt caacaagcgc agggacgagt tccggcaagg ggagctgaag
     7681 tcgagcagct gcattagtgg agtgctcact ggctagcctc aaggcaaggc aatgaagctg
     7741 cttcggtatg ctacttgatg ctacatgcct gtacagatat tcttggattt tggcatttct
     7801 agtcctttgt tcgagtagag tgaccatttc tctgagcttg agagcaaaag ttttcgcgtc
     7861 taatcttgtg ttcttgactt cgtccataaa ttcctctaat gtttgaggaa tatcactcct
     7921 tccctttaat tcatcattgc caagtggttg ttctagcact tgaaacataa cctctggtac
     7981 agccgagtca agccttctcc ccaatatcct tggccctagt cttttcccca agcagcctag
     8041 tgtagagcac ttggtttctc catcaatgga gtcaactgtg gacaaaacga acacaaaccg
     8101 gagaagaaac gtgaagaaca agagagaata gaagagcatt cgataagaaa cccttctagc
     8161 tccaaccctc actttgataa actctctgac accttttcca ggaaaaactg taacatgcct
     8221 caaactcggc gatatatgaa gctgcatccc gggggtgaag ggggcggccg cggagcctgc
     8281 ttttttgtac aaacttgtga tactcctagg ctcgagcccg tgaaagagag agtcgcgaga
     8341 gatttgcaga gatcgcttta ggctttggga gagattgaag agtcagaaaa agacgaaagg
     8401 atgaattatt atcttccaca cgaaggtctt ctttatatcg caaaccaaaa gcccaaaacc
     8461 gtcttttcta ttaatgagaa taaaatatct ttagccaaaa caaaaaaagg aagatatcag
     8521 ttgaggatta ttatcacgaa actaaaggaa ggaatcatat gatacgtgtc tattttccac
     8581 cgtgcgtttt taaaagaccg actcaagtag aaacatccta tggtggtggt tggattaggt
     8641 catccattac atctgcttca ctgacatttt tctatttttc tttttgtata tacttttcct
     8701 caaataattt ctttcttttc tatagaagaa tttaatcaat aaggaaaaag ttcaaaaaag
     8761 attctttcca ttaagactat gtcttggtta acccaaccca ttaagaataa gcaatcataa
     8821 tatatataga gaatactaat actatatatg agatttttct tttaatttca tgttgattat
     8881 gatagtttat cttcttgatt taatttatca atacttggca taaaagattc taatctactc
     8941 taataaagaa aagaaaaaaa agtatctacc attgactaat taaaataagg aaacttatct
     9001 accaaatttg agtatttttt agaacaatct ttttggttta attccaaaac tctaaaccta
     9061 attgttggga aaaaggacct aatttttaag aaaagttaat aattagaaga tctgtatgtt
     9121 tttttttttg atccaagttt ttatttcttt tctctttttt tcatgataaa atctatgttt
     9181 ttttagtcta caattaaagt aattgttatt attttcttta tctttttttg ttgttgttgt
     9241 taattccctt tttttttttt taacagcaac ttcttaaaaa aaaaaacagt tgggccttga
     9301 atttatttca ggcctgcgtt attaagccca gataataact caaaacaaaa aaaatgttga
     9361 accggaataa acccgcgaga ttaaatgccg gttttcaggt aacatagaag aagaatatat
     9421 gaggattgaa gaagtattca agaggcggaa caattcacaa gtccaagagc ttaaatttct
     9481 cctcactctt ctgctacaga ctcggaactc tttctctttg ctaaaataag atgttcagga
     9541 tttttgttgc ccgacaattc atgtatctca cactctctct cttctctgtt cttactactc
     9601 tgttacatta ccaccaactc aagactttct tccacaatgg cgtttatgag acttggctcc
     9661 aaatccgaag cttatcgata ccgtcgacct cgagagcccg ggctcctgca ggtaccttaa
     9721 ttaaaagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taa
//
