LOCUS       40924_4125       10459 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAGRIKOLA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Hilson P, Allemeersch J, Altmann T, Aubourg S, Avon A, Beynon J, 
            Bhalerao RP, Bitton F, Caboche M, Cannoot B, Chardakov V, 
            Cognet-Holliger C, Colot V, Crowe M, Darimont C, Durinck S, Eickhoff
            H, de Longevialle AF, Farmer EE, Grant M, Kuiper MT, Lehrach H, Leon
            C, Leyva A, Lundeberg J, Lurin C, Moreau Y, Nietfeld W, Paz-Ares J, 
            Reymond P, Rouze P, Sandberg G, Segura MD, Serizet C, Tabrett A, 
            Taconnat L, Thareau V, Van Hummelen P, Vercruysse S, Vuylsteke M, 
            Weingartner M, Weisbeek PJ, Wirta V, Wittink FR, Zabeau M, Small I.
  TITLE     Versatile gene-specific sequence tags for Arabidopsis functional 
            genomics: transcript profiling and reverse genetics applications
  JOURNAL   Genome Res. 14 (10B), 2176-2189 (2004)
  PUBMED    15489341
REFERENCE   2  (bases 1 to 10459)
  AUTHORS   Small ID, Hilson P.
  TITLE     pAGRIKOLA sequence
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 10459)
  AUTHORS   Small ID, Hilson P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2004) URGV, INRA/CNRS/UEVE, 2, rue Gaston 
            Cremieux, CP5708, Evry, Essonne 91057, France
REFERENCE   4  (bases 1 to 10459)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 10459)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            14 (10B), 2176-2189 (2004)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (07-MAR-2004) URGV, INRA/CNRS/UEVE, 2, rue Gaston Cremieux, CP5708, 
            Evry, Essonne 91057, France"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      18..672
                     /label=colE1
                     /note="colE1"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(823..1635)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1926..2361
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2488..2510
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     terminator      complement(2534..2786)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2809..3357)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(3399..3578)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     3825..3841
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3851..3869
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3895..3911
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3945..3961)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4968..5313
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5322..5446
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(5878..6180)
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(6619..6743)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          6770..7538
                     /label=PDK intron
                     /note="pyruvate orthophosphate dikinase intron from
                     Flaveria trinervia"
     intron          complement(7573..7762)
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     protein_bind    7786..7910
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(8242..8544)
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(9075..9199)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      9208..9915
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(9956..9974)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(10013..10031)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10052..10068)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10076..10092
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10100..10130)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10145..10166)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    10405..10429
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      541 cttcggaaga agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      781 atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat
      841 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      901 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      961 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     1021 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     1081 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     1141 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     1201 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     1261 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     1321 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     1381 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     1441 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     1501 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     1561 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
     1621 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     1681 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     1741 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     1801 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     1861 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     1921 gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata
     1981 gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt
     2041 ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa
     2101 tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga
     2161 gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt
     2221 aacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt
     2281 cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag
     2341 cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt
     2401 ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct
     2461 ggatttccga tccccggaat tagatcttgg caggatatat tgtggtgtaa cgttatcagc
     2521 ttgcatgccg gtcgatctag taacatagat gacaccgcgc gcgataattt atcctagttt
     2581 gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata
     2641 aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa
     2701 ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa
     2761 ctttattgcc aaatgtttga acgatctgct tgactctagg ggtcatcaga tttcggtgac
     2821 gggcaggacc ggacggggcg gcaccggcag gctgaagtcc agctgccaga aacccacgtc
     2881 atgccagttc ccgtgcttga agccggccgc ccgcagcatg ccgcgggggg catatccgag
     2941 cgcctcgtgc atgcgcacgc tcgggtcgtt gggcagcccg atgacagcga ccacgctctt
     3001 gaagccctgt gcctccaggg acttcagcag gtgggtgtag agcgtggagc ccagtcccgt
     3061 ccgctggtgg cggggggaga cgtacacggt cgactcggcc gtccagtcgt aggcgttgcg
     3121 tgccttccag ggacccgcgt aggcgatgcc ggcgacctcg ccgtccacct cggcgacgag
     3181 ccagggatag cgctcccgca gacggacgag gtcgtccgtc cactcctgcg gttcctgcgg
     3241 ctcggtacgg aagttgaccg tgcttgtctc gatgtagtgg ttgacgatgg tgcagaccgc
     3301 cggcatgtcc gcctcggtgg cacggcggat gtcggccggg cgtcgttctg ggctcatggt
     3361 agatcccctc gatcgagttg agagtgaata tgagactcta attggatacc gaggggaatt
     3421 tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata gtgaccttag
     3481 gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat taaactccag
     3541 aaacccgcgg ctcagtggct ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg
     3601 gcttgtcccg cgtcatcggc gggggtcata acgtgactcc cttaattctc cgctcatgat
     3661 cgataacatt aacgcttaca atttccattc gccattcagg ctgcgcaact gttgggaagg
     3721 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     3781 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     3841 tgagcgcgcg taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt
     3901 cgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatcca ctagttctag
     3961 agcggccgct cgacgaatta attccaatcc cacaaaaatc tgagcttaac agcacagttg
     4021 ctcctctcag agcagaatcg ggtattcaac accctcatat caactactac gttgtgtata
     4081 acggtccaca tgccggtata tacgatgact ggggttgtac aaaggcggca acaaacggcg
     4141 ttcccggagt tgcacacaag aaatttgcca ctattacaga ggcaagagca gcagctgacg
     4201 cgtacacaac aagtcagcaa acagacaggt tgaacttcat ccccaaagga gaagctcaac
     4261 tcaagcccaa gagctttgct aaggccctaa caagcccacc aaagcaaaaa gcccactggc
     4321 tcacgctagg aaccaaaagg cccagcagtg atccagcccc aaaagagatc tcctttgccc
     4381 cggagattac aatggacgat ttcctctatc tttacgatct aggaaggaag ttcgaaggtg
     4441 aaggtgacga cactatgttc accactgata atgagaaggt tagcctcttc aatttcagaa
     4501 agaatgctga cccacagatg gttagagagg cctacgcagc aggtctcatc aagacgatct
     4561 acccgagtaa caatctccag gagatcaaat accttcccaa gaaggttaaa gatgcagtca
     4621 aaagattcag gactaattgc atcaagaaca cagagaaaga catatttctc aagatcagaa
     4681 gtactattcc agtatggacg attcaaggct tgcttcataa accaaggcaa gtaatagaga
     4741 ttggagtctc taaaaaggta gttcctactg aatctaaggc catgcatgga gtctaagatt
     4801 caaatcgagg atctaacaga actcgccgtg aagactggcg aacagttcat acagagtctt
     4861 ttacgactca atgacaagaa gaaaatcttc gtcaacatgg tggagcacga ccctctggtc
     4921 taccccaaaa atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa
     4981 caaaggataa tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc
     5041 gaaaggacag tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag
     5101 gctatcattc aagatctctc tgccgacagt ggtcccaaag atggaccccc acccacgagg
     5161 agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac
     5221 atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct
     5281 atataaggaa gttcatttca tttggagagg acacgctcga gacaagtttg tacaaaaaag
     5341 ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
     5401 aacagactac ataatactgt aaaacacaac atatccagtc actatgaatc aactacttag
     5461 atggtattag tgacctgtag tcgaccgaca gccttccaaa tgttcttcgg gtgatgctgc
     5521 caacttagtc gaccgacagc cttccaaatg ttcttctcaa acggaatcgc cgtatccagc
     5581 ctactcgcta ttgtcctcaa tgccgtatta aatcataaaa agaaataaga aaaagaggtg
     5641 cgagcctctt ttttgtgtga caaaataaaa acatctacct attcatatac gctagtgtca
     5701 tagtcctgaa aatcatctgc atcaagaaca atttcacaac tcttatactt ttctcttaca
     5761 agtcgttcgg cttcatctgg attttcagcc tctatactta ctaaacgtga taaagtttct
     5821 gtaatttcta ctgtatcgac ctgcagactg gctgtgtata agggagcctg acatttatat
     5881 tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc
     5941 cacttcttcc ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca
     6001 gctttcatcc ccgatatgca ccaccgggta aagttcacgg gagactttat ctgacagcag
     6061 acgtgcactg gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg
     6121 tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat
     6181 ttcaccagcc cctgttctcg tcagcaaaag agccgttcat ttcaataaac cgggcgacct
     6241 cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat
     6301 tctgcatggt tgtgcttacc agaccggaga tattgacatc atatatgcct tgagcaactg
     6361 atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcatacc tctttttgac
     6421 atacttcggg tgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg
     6481 gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt
     6541 tattcggcgc aaagtgcgtc gggtgatgct gccaacttag tcgactacag gtcactaata
     6601 ccatctaagt agttgattca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
     6661 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
     6721 tcagctttct tgtacaaagt ggtctcgagg aattcggtac cccagcttgg taaggaaata
     6781 attattttct tttttccttt tagtataaaa tagttaagtg atgttaatta gtatgattat
     6841 aataatatag ttgttataat tgtgaaaaaa taatttataa atatattgtt tacataaaca
     6901 acatagtaat gtaaaaaaat atgacaagtg atgtgtaaga cgaagaagat aaaagttgag
     6961 agtaagtata ttatttttaa tgaatttgat cgaacatgta agatgatata ctagcattaa
     7021 tatttgtttt aatcataata gtaattctag ctggtttgat gaattaaata tcaatgataa
     7081 aatactatag taaaaataag aataaataaa ttaaaataat atttttttat gattaatagt
     7141 ttattatata attaaatatc tataccatta ctaaatattt tagtttaaaa gttaataaat
     7201 attttgttag aaattccaat ctgcttgtaa tttatcaata aacaaaatat taaataacaa
     7261 gctaaagtaa caaataatat caaactaata gaaacagtaa tctaatgtaa caaaacataa
     7321 tctaatgcta atataacaaa gcgcaagatc tatcaatttt atatagtatt atttttcaat
     7381 caacattctt attaatttct aaataatact tgtagtttta ttaacttcta aatggattga
     7441 ctattaatta aatgaattag tcgaacatga ataaacaagg taacatgata gatcatgtca
     7501 ttgtgttatc attgatctta catttggatt gattacagtt gggaagctgg gttcgaaatc
     7561 gataagcttg cgctgcagtt atcatcatca tcatagacac acgaaataaa gtaatcagat
     7621 tatcagttaa agctatgtaa tatttacacc ataaccaatc aattaaaaaa tagatcagtt
     7681 taaagaaaga tcaaagctca aaaaaataaa aagagaaaag ggtcctaacc aagaaaatga
     7741 aggagaaaaa ctagaaattt acctgcacaa gcttggatcc tctagaccac tttgtacaag
     7801 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
     7861 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg aatcaactac
     7921 ttagatggta ttagtgacct gtagtcgact aagttggcag catcacccga cgcactttgc
     7981 gccgaataaa tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct
     8041 gttgataccg ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggatttca
     8101 caactcttat acttttctct tacaagtcgt tcggcttcat ctggattttc agcctctata
     8161 cttactaaac gtgataaagt ttctgtaatt tctactgtat cgacctgcag actggctgtg
     8221 tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc
     8281 attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc
     8341 catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc
     8401 acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc
     8461 gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt
     8521 ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcagca aaagagccgt
     8581 tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt
     8641 cggcacgcag acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga
     8701 catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg
     8761 cttcatagca tacctctttt tgacatactt cgggtatgat gcagatgatt ttcaggacta
     8821 tgacactagc gtatatgaat aggtagatgt ttttattttg tcacacaaaa aagaggctcg
     8881 cacctctttt tcttatttct ttttatgatt taatacggca ttgaggacaa tagcgagtag
     8941 gctggatacg acgattccgt ttgagaagaa catttggaag gctgtcggtc gactaagttg
     9001 gcagcatcac ccgaagaaca tttggaaggc tgtcggtcga ctacaggtca ctaataccat
     9061 ctaagtagtt gattcatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
     9121 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
     9181 cttttttgta caaacttgtc tagagtcctg ctttaatgag atatgcgaga cgcctatgat
     9241 cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc
     9301 tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt tactatcgta
     9361 tttttatgaa taatattctc cgttcaattt actgattgta ccctactact tatatgtaca
     9421 atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc tatgatagag
     9481 cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa aaaagcggca
     9541 gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca aaagtgcccc
     9601 aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg aagggaactc
     9661 cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg ccgtccgctc
     9721 taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt aaccgacttg
     9781 ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg aagtgcaggt
     9841 caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat
     9901 gtcgaggctc agcaggacct gcaggcatgc aagctagctt actagtgatg catattctat
     9961 agtgtcacct aaatctgcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
    10021 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
    10081 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    10141 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    10201 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
    10261 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    10321 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    10381 acgcaggaaa gaacatgaag gccttgacag gatatattgg cgggtaaact aagtcgctgt
    10441 atgtgtttgt ttgagatct
//
