LOCUS       62056_2935        6416 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     237..253
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        366..726
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             731..1753
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      1926..2493
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus
                     nidulans trpC gene"
     misc_feature    2950..2974
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      3113..3744
                     /label=oriV
                     /note="incP origin of replication"
     CDS             4026..4817
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             5119..6264
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    6393..6416
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt actggggtgg
       61 tttttctttt caccagtgag acgggcaaca gcggcgccat tcgccattca ggctgcgcaa
      121 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
      181 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
      241 aacgacggcc agtgaattcg agctcggtac caaggcccgg gctggccacg gccgcctagg
      301 cgcgcaagga tcctctagat ctcgaggcct gatcatcgat gggcccatcg atgatcaggc
      361 ctcgacagaa gatgatattg aaggagcact ttttgggctt ggctggagct agtggaggtc
      421 aacaatgaat gcctattttg gtttagtcgt ccaggcggtg agcacaaaat ttgtgtcgtt
      481 tgacaagatg gttcatttag gcaactggtc agatcagccc cacttgtagc agtagcggcg
      541 gcgctcgaag tgtgactctt attagcagac aggaacgagg acattattat catctgctgc
      601 ttggtgcacg ataacttggt gcgtttgtca agcaaggtaa gtgaacgacc cggtcatacc
      661 ttcttaagtt cgcccttcct ccctttattt cagattcaat ctgacttacc tattctaccc
      721 aagcatcgat atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
      781 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
      841 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
      901 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
      961 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     1021 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     1081 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     1141 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     1201 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     1261 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     1321 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     1381 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     1441 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     1501 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     1561 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     1621 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     1681 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     1741 gagggcaaag gaatagagta gatgccgacc gggatccact taacgttact gaaatcatca
     1801 aacagcttga cgaatctgga tataagatcg ttggtgtcga tgtcagctcc ggagttgaga
     1861 caaatggtgt tcaggatctc gataagatac gttcatttgt ccaagcagca aagagtgcct
     1921 tctagtgatt taatagctcc atgtcaacaa gaataaaacg cgtttcgggt ttacctcttc
     1981 cagatacagc tcatctgcaa tgcattaatg cattggacct cgcaacccta gtacgccctt
     2041 caggctccgg cgaagcagaa gaatagctta gcagagtcta ttttcatttt cgggagacga
     2101 gatcaagcag atcaacggtc gtcaagagac ctacgagact gaggaatccg ctcttggctc
     2161 cacgcgacta tatatttgtc tctaattgta ctttgacatg ctcctcttct ttactctgat
     2221 agcttgacta tgaaaattcc gtcaccagcc cctgggttcg caaagataat tgcactgttt
     2281 cttccttgaa ctctcaagcc tacaggacac acattcatcg taggtataaa cctcgaaaat
     2341 cattcctact aagatgggta tacaatagta accatggttg cctagtgaat gctccgtaac
     2401 acccaatacg ccggccgaaa cttttttaca actctcctat gagtcgttta cccagaatgc
     2461 acaggtacac ttgtttagag gtaatccttc tttctagagg atcctctacg ccggacgcat
     2521 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
     2581 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
     2641 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
     2701 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
     2761 gcataaggga gagcgtcgag atctagagga tcccccgact agtgcgcgat cgcggccggc
     2821 cggcgcgccg tttaaacgga tttaaattaa ttaatgtcga cctgcaggca tgcaagcttc
     2881 gtgactccct taattctccg ctcatgatca gattgtcgtt tcccgccttc agtttaaact
     2941 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     3001 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tttgttcatg
     3061 ccaaccacag ggttccagat ccgacgagca aggcaagacc gagcgccttt gcgacgctca
     3121 ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag
     3181 aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg
     3241 aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac
     3301 ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc
     3361 cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga
     3421 caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat
     3481 gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat
     3541 tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt
     3601 ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg
     3661 tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc
     3721 cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggc gtacgccact
     3781 ggagcacctc aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat
     3841 tgtggtttca aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt
     3901 gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc
     3961 gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat
     4021 aataaatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct
     4081 gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg
     4141 aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac
     4201 gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact
     4261 ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct
     4321 cggaagagta tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca
     4381 tcaggctctt tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc
     4441 gcttagccga attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact
     4501 gggaagaaga cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa
     4561 agcccgaaga ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga
     4621 aagatggcaa agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt
     4681 atgacattgc cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg
     4741 agctattttt tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt
     4801 tactggatga attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag
     4861 cgcaccgact tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat
     4921 ttgggcaagg ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag
     4981 gacggccaga cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc
     5041 aaggcaccag gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca
     5101 atcccgcaag gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg
     5161 atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt
     5221 gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc
     5281 gagcgcgaca gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag
     5341 cgttcgcgtc gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg
     5401 cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc
     5461 agcgaggcca agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag
     5521 ctttccttgt tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg
     5581 gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac
     5641 aaggtcattt tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg
     5701 gccgacgatg acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc
     5761 ggcgagccga tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat
     5821 ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc
     5881 ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc
     5941 ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg
     6001 ctgtttgctg gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg
     6061 acggcccgac ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg
     6121 gaaaccttcc gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag
     6181 gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat
     6241 gatgacctgg tgcattgcaa acgctagggc cttgtggggt cagttccggc tggatctgct
     6301 ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
     6361 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaa
//
