LOCUS       62056_2930        6170 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     237..253
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        366..726
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             731..1522
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      1680..2247
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus
                     nidulans trpC gene"
     misc_feature    2704..2728
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      2867..3498
                     /label=oriV
                     /note="incP origin of replication"
     CDS             3780..4571
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             4873..6018
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    6147..6170
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt actggggtgg
       61 tttttctttt caccagtgag acgggcaaca gcggcgccat tcgccattca ggctgcgcaa
      121 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
      181 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
      241 aacgacggcc agtgaattcg agctcggtac caaggcccgg gctggccacg gccgcctagg
      301 cgcgcaagga tcctctagat ctcgaggcct gatcatcgat gggcccatcg atgatcaggc
      361 ctcgacagaa gatgatattg aaggagcact ttttgggctt ggctggagct agtggaggtc
      421 aacaatgaat gcctattttg gtttagtcgt ccaggcggtg agcacaaaat ttgtgtcgtt
      481 tgacaagatg gttcatttag gcaactggtc agatcagccc cacttgtagc agtagcggcg
      541 gcgctcgaag tgtgactctt attagcagac aggaacgagg acattattat catctgctgc
      601 ttggtgcacg ataacttggt gcgtttgtca agcaaggtaa gtgaacgacc cggtcatacc
      661 ttcttaagtt cgcccttcct ccctttattt cagattcaat ctgacttacc tattctaccc
      721 aagcatcgat atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
      781 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
      841 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
      901 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
      961 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     1021 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     1081 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     1141 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     1201 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     1261 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     1321 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     1381 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     1441 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     1501 tcgccttctt gacgagttct tctgaggatc cacttaacgt tactgaaatc atcaaacagc
     1561 ttgacgaatc tggatataag atcgttggtg tcgatgtcag ctccggagtt gagacaaatg
     1621 gtgttcagga tctcgataag atacgttcat ttgtccaagc agcaaagagt gccttctagt
     1681 gatttaatag ctccatgtca acaagaataa aacgcgtttc gggtttacct cttccagata
     1741 cagctcatct gcaatgcatt aatgcattgg acctcgcaac cctagtacgc ccttcaggct
     1801 ccggcgaagc agaagaatag cttagcagag tctattttca ttttcgggag acgagatcaa
     1861 gcagatcaac ggtcgtcaag agacctacga gactgaggaa tccgctcttg gctccacgcg
     1921 actatatatt tgtctctaat tgtactttga catgctcctc ttctttactc tgatagcttg
     1981 actatgaaaa ttccgtcacc agcccctggg ttcgcaaaga taattgcact gtttcttcct
     2041 tgaactctca agcctacagg acacacattc atcgtaggta taaacctcga aaatcattcc
     2101 tactaagatg ggtatacaat agtaaccatg gttgcctagt gaatgctccg taacacccaa
     2161 tacgccggcc gaaacttttt tacaactctc ctatgagtcg tttacccaga atgcacaggt
     2221 acacttgttt agaggtaatc cttctttcta gaggatcctc tacgccggac gcatcgtggc
     2281 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     2341 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     2401 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
     2461 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     2521 gggagagcgt cgagatctag aggatccccc gactagtgcg cgatcgcggc cggccggcgc
     2581 gccgtttaaa cggatttaaa ttaattaatg tcgacctgca ggcatgcaag cttcgtgact
     2641 cccttaattc tccgctcatg atcagattgt cgtttcccgc cttcagttta aactatcagt
     2701 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     2761 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtttgtt catgccaacc
     2821 acagggttcc agatccgacg agcaaggcaa gaccgagcgc ctttgcgacg ctcaccgggc
     2881 tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc
     2941 cgtcgaagcc gtgtgcgaga caccgcggcc gccggcgttg tggatacctc gcggaaaact
     3001 tggccctcac tgacagatga ggggcggacg ttgacacttg aggggccgac tcacccggcg
     3061 cggcgttgac agatgagggg caggctcgat ttcggccggc gacgtggagc tggccagcct
     3121 cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc acagatgatg tggacaagcc
     3181 tggggataag tgccctgcgg tattgacact tgaggggcgc gactactgac agatgagggg
     3241 cgcgatcctt gacacttgag gggcagagtg ctgacagatg aggggcgcac ctattgacat
     3301 ttgaggggct gtccacaggc agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg
     3361 gccaccgcta acctgtcttt taacctgctt ttaaaccaat atttataaac cttgttttta
     3421 accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc
     3481 gaaccctccc ggcccgctaa cgcgggcctc ccatcccccc aggcgtacgc cactggagca
     3541 cctcaaaaac accatcatac actaaatcag taagttggca gcatcaccca taattgtggt
     3601 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     3661 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     3721 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     3781 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     3841 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     3901 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     3961 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     4021 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     4081 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     4141 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     4201 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     4261 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     4321 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     4381 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     4441 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     4501 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     4561 atgaattgtt ttagtaccta gatgtggcgc aacgatgccg gcgacaagca ggagcgcacc
     4621 gacttcttcc gcatcaagtg ttttggctct caggccgagg cccacggcaa gtatttgggc
     4681 aaggggtcgc tggtattcgt gcagggcaag attcggaata ccaagtacga gaaggacggc
     4741 cagacggtct acgggaccga cttcattgcc gataaggtgg attatctgga caccaaggca
     4801 ccaggcgggt caaatcagga ataagggcac attgccccgg cgtgagtcgg ggcaatcccg
     4861 caaggagggt gaatgaatcg gacgtttgac cggaaggcat acaggcaaga actgatcgac
     4921 gcggggtttt ccgccgagga tgccgaaacc atcgcaagcc gcaccgtcat gcgtgcgccc
     4981 cgcgaaacct tccagtccgt cggctcgatg gtccagcaag ctacggccaa gatcgagcgc
     5041 gacagcgtgc aactggctcc ccctgccctg cccgcgccat cggccgccgt ggagcgttcg
     5101 cgtcgtctcg aacaggaggc ggcaggtttg gcgaagtcga tgaccatcga cacgcgagga
     5161 actatgacga ccaagaagcg aaaaaccgcc ggcgaggacc tggcaaaaca ggtcagcgag
     5221 gccaagcagg ccgcgttgct gaaacacacg aagcagcaga tcaaggaaat gcagctttcc
     5281 ttgttcgata ttgcgccgtg gccggacacg atgcgagcga tgccaaacga cacggcccgc
     5341 tctgccctgt tcaccacgcg caacaagaaa atcccgcgcg aggcgctgca aaacaaggtc
     5401 attttccacg tcaacaagga cgtgaagatc acctacaccg gcgtcgagct gcgggccgac
     5461 gatgacgaac tggtgtggca gcaggtgttg gagtacgcga agcgcacccc tatcggcgag
     5521 ccgatcacct tcacgttcta cgagctttgc caggacctgg gctggtcgat caatggccgg
     5581 tattacacga aggccgagga atgcctgtcg cgcctacagg cgacggcgat gggcttcacg
     5641 tccgaccgcg ttgggcacct ggaatcggtg tcgctgctgc accgcttccg cgtcctggac
     5701 cgtggcaaga aaacgtcccg ttgccaggtc ctgatcgacg aggaaatcgt cgtgctgttt
     5761 gctggcgacc actacacgaa attcatatgg gagaagtacc gcaagctgtc gccgacggcc
     5821 cgacggatgt tcgactattt cagctcgcac cgggagccgt acccgctcaa gctggaaacc
     5881 ttccgcctca tgtgcggatc ggattccacc cgcgtgaaga agtggcgcga gcaggtcggc
     5941 gaagcctgcg aagagttgcg aggcagcggc ctggtggaac acgcctgggt caatgatgac
     6001 ctggtgcatt gcaaacgcta gggccttgtg gggtcagttc cggctggatc tgctctcccg
     6061 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
     6121 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa
//
