LOCUS       40924_4031        7069 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pADbeta, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disc version 1.3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   MacGregor GR, Caskey CT.
  TITLE     Construction of plasmids that express E. coli beta-galactosidase in 
            mammalian cells
  JOURNAL   Nucleic Acids Res. 17 (6), 2365 (1989)
  PUBMED    2495524
REFERENCE   3  (bases 1 to 7069)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE   4  (bases 1 to 7069)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7069)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1989"; volume: "17"; issue: "6"; pages: "2365"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East Meadow Circle, Palo Alto, CA 94303, USA. To place an order call
            (415) 424-8222 or (800) 662-2566, extension 1. International 
            customers, please contact your local distributor.  For technical 
            information, call (415) 424- 8222 or (800) 662-2566, extension 3. 
            This sequence has been compiled from information in the sequence 
            databases, published literature and other sources, together with 
            partial sequences obtained by CLONTECH; this vector has not been 
            completely sequenced. If you suspect there is an error in this 
            sequence, please contact CLONTECH's Technical Service Department at 
            (415) 424-8222 or (800) 662-2566, extension 3 or E-mail 
            TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..7069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          459..688
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     CDS             874..3918
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     polyA_signal    complement(4208..4342)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4453..4469)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4477..4493)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4501..4531)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4546..4567)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4855..5443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5617..6474)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6475..6579)
                     /label=AmpR promoter
     primer_bind     7053..7069
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcgagc tcggtactcg agcggtgttc cgcggtcctc ctcgtataga aactcggacc
       61 actctgagac gaaggctcgc gtccaggcca gcacgaagga ggctaagtgg gaggggtagc
      121 ggtcgttgtc cactaggggg tccactcgct ccagggtgtg aagacacatg tcgccctctt
      181 cggcatcaag gaaggtgatt ggtttatagg tgtaggccac gtgaccgggt gttcctgaag
      241 gggggctata aaagggggtg ggggcgcgtt cgtcctcact ctcttccgca tcgctgtctg
      301 cgagggccag ctgttgggct cgcggttgag gacaaactct tcgcggtctt tccagtactc
      361 ttggatcgga aacccgtcgg cctccgaacg gtactccgcc accgagggac ctgagcgagt
      421 ccgcatcgac cggatcggaa aacctctcga ctgttggggt gagtactccc tctcaaaagc
      481 gggcatgact tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt tgatattcac
      541 ctggcccgcg gtgatgcctt tgagggtggc cgcgtccatc tggtcagaaa agacaatctt
      601 tttgttgtca agcttgaggt gtggcaggct tgagatctgg ccatacactt gagtgacaat
      661 gacatccact ttgcctttct ctccacaggt gtccactccc aggtccaacc ggaattgtac
      721 ccgcggccgc aattcccggg gatcgaaaga gcctgctaaa gcaaaaaaga agtcaccatg
      781 tcgtttactt tgaccaacaa gaacgtgatt ttcgttgccg gtctgggagg cattggtctg
      841 gacaccagca aggagctgct caagcgcgat cccgtcgttt tacaacgtcg tgactgggaa
      901 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      961 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     1021 tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat
     1081 cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg
     1141 cccatctaca ccaacgtaac ctatcccatt acggtcaatc cgccgtttgt tcccacggag
     1201 aatccgacgg gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc
     1261 cagacgcgaa ttatttttga tggcgttaac tcggcgtttc atctgtggtg caacgggcgc
     1321 tgggtcggtt acggccagga cagtcgtttg ccgtctgaat ttgacctgag cgcattttta
     1381 cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgtt ggagtgacgg cagttatctg
     1441 gaagatcagg atatgtggcg gatgagcggc attttccgtg acgtctcgtt gctgcataaa
     1501 ccgactacac aaatcagcga tttccatgtt gccactcgct ttaatgatga tttcagccgc
     1561 gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc gtgactacct acgggtaaca
     1621 gtttctttat ggcagggtga aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa
     1681 attatcgatg agcgtggtgg ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac
     1741 ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg cggtggttga actgcacacc
     1801 gccgacggca cgctgattga agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt
     1861 gaaaatggtc tgctgctgct gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac
     1921 gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca ggatatcctg
     1981 ctgatgaagc agaacaactt taacgccgtg cgctgttcgc attatccgaa ccatccgctg
     2041 tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa tattgaaacc
     2101 cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc gctggctacc ggcgatgagc
     2161 gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg
     2221 ctggggaatg aatcaggcca cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct
     2281 gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag ccgacaccac ggccaccgat
     2341 attatttgcc cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa
     2401 tggtccatca aaaaatggct ttcgctacct ggagagacgc gcccgctgat cctttgcgaa
     2461 tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta aatactggca ggcgtttcgt
     2521 cagtatcccc gtttacaggg cggcttcgtc tgggactggg tggatcagtc gctgattaaa
     2581 tatgatgaaa acggcaaccc gtggtcggct tacggcggtg attttggcga tacgccgaac
     2641 gatcgccagt tctgtatgaa cggtctggtc tttgccgacc gcacgccgca tccagcgctg
     2701 acggaagcaa aacaccagca gcagtttttc cagttccgtt tatccgggca aaccatcgaa
     2761 gtgaccagcg aatacctgtt ccgtcatagc gataacgagc tcctgcactg gatggtggcg
     2821 ctggatggta agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa
     2881 cagttgattg aactgcctga actaccgcag ccggagagcg ccgggcaact ctggctcaca
     2941 gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg
     3001 cagcagtggc gtctggcgga aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc
     3061 ccgcatctga ccaccagcga aatggatttt tgcatcgagc tgggtaataa gcgttggcaa
     3121 tttaaccgcc agtcaggctt tctttcacag atgtggattg gcgataaaaa acaactgctg
     3181 acgccgctgc gcgatcagtt cacccgtgca ccgctggata acgacattgg cgtaagtgaa
     3241 gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg ccattaccag
     3301 gccgaagcag cgttgttgca gtgcacggca gatacacttg ctgatgcggt gctgattacg
     3361 accgctcacg cgtggcagca tcaggggaaa accttattta tcagccggaa aacctaccgg
     3421 attgatggta gtggtcaaat ggcgattacc gttgatgttg aagtggcgag cgatacaccg
     3481 catccggcgc ggattggcct gaactgccag ctggcgcagg tagcagagcg ggtaaactgg
     3541 ctcggattag ggccgcaaga aaactatccc gaccgcctta ctgccgcctg ttttgaccgc
     3601 tgggatctgc cattgtcaga catgtatacc ccgtacgtct tcccgagcga aaacggtctg
     3661 cgctgcggga cgcgcgaatt gaattatggc ccacaccagt ggcgcggcga cttccagttc
     3721 aacatcagcc gctacagtca acagcaactg atggaaacca gccatcgcca tctgctgcac
     3781 gcggaagaag gcacatggct gaatatcgac ggtttccata tggggattgg tggcgacgac
     3841 tcctggagcc cgtcagtatc ggcggaatta cagctgagcg ccggtcgcta ccattaccag
     3901 ttggtctggt gtcaaaaata ataataaccg ggcaggccat gtctgcccgt atttcgcgta
     3961 aggaaatcca ttatgtacta tttaaaaaac acaaactttt ggatgttcgg tttattcttt
     4021 ttcttttact tttttatcat gggagcctac ttcccgtttt tcccgatttg gctacatgac
     4081 atcaaccata tcagcaaaag tgatacgggt attatttttg ccgctatttc tctgttctcg
     4141 ctattattcc aaccgctgtt tggtctgctt tctgacaaac tcggcctcga ctctaggcgg
     4201 ccgcggggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     4261 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     4321 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     4381 gggggaggtg tgggaggttt tttcggatcc tctagagtcg acctgcaggc atgcaagctt
     4441 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4501 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     4561 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     4621 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     4681 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4741 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4801 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4861 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4921 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4981 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5041 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5101 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5161 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5221 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5281 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5341 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5401 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5461 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5521 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5581 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5641 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5701 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5761 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     5821 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5881 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5941 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6001 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6061 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6121 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6181 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6241 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6301 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6361 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6421 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6481 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6541 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6601 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     6661 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     6721 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     6781 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     6841 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     6901 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     6961 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     7021 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagt
//
