LOCUS       V014188                 8155 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014188
VERSION     V014188
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(22..402)
                     /direction=LEFT
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     repeat_region   440..542
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     630..780
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        838..1141
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1142..1345
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1413..2105
                     /label="ZsGreen1"
                     /note="Zoanthus green fluorescent protein"
     polyA_signal    2241..2362
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             2456..2875
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(2941..4275)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     CDS             complement(4860..5243)
                     /note="Early 4 ORF1 protein from Human adenovirus C
                     serotype 2. Accession#: P03242"
                     /label="Early 4 ORF1 protein"
     repeat_region   5553..5655
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     rep_origin      complement(5915..6503)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             7335..8126
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
       61 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      121 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      181 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      241 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      301 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      361 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggat
      421 cgaattaatt cttaattaac atcatcaata atatacctta ttttggattg aagccaatat
      481 gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt
      541 agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa cacatgtaag cgacggatgt
      601 ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg
      661 ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt aagatttggc cattttcgcg
      721 ggaaaactga ataagaggaa gtgaaatctg aataattttg tgttactcat agcgcgtaat
      781 actgtaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      841 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      901 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      961 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     1021 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     1081 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1141 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1201 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     1261 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     1321 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agagatctgg
     1381 taccgtcgac gcggccgctc gagcctaagc ttatggccca gtccaagcac ggcctgacca
     1441 aggagatgac catgaagtac cgcatggagg gctgcgtgga cggccacaag ttcgtgatca
     1501 ccggcgaggg catcggctac cccttcaagg gcaagcaggc catcaacctg tgcgtggtgg
     1561 agggcggccc cttgcccttc gccgaggaca tcttgtccgc cgccttcatg tacggcaacc
     1621 gcgtgttcac cgagtacccc caggacatcg tcgactactt caagaactcc tgccccgccg
     1681 gctacacctg ggaccgctcc ttcctgttcg aggacggcgc cgtgtgcatc tgcaacgccg
     1741 acatcaccgt gagcgtggag gagaactgca tgtaccacga gtccaagttc tacggcgtga
     1801 acttccccgc cgacggcccc gtgatgaaga agatgaccga caactgggag ccctcctgcg
     1861 agaagatcat ccccgtgccc aagcagggca tcttgaaggg cgacgtgagc atgtacctgc
     1921 tgctgaagga cggtggccgc ttgcgctgcc agttcgacac cgtgtacaag gccaagtccg
     1981 tgccccgcaa gatgcccgac tggcacttca tccagcacaa gctgacccgc gaggaccgca
     2041 gcgacgccaa gaaccagaag tggcacctga ccgagcacgc catcgcctcc ggctccgcct
     2101 tgccctgaat ccgatccacc ggatctagat aactgatcat aatcagccat accacatttg
     2161 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     2221 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     2281 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     2341 ccaaactcat caatgtatct taacgcggat ctgggcgtgg ttaagggtgg gaaagaatat
     2401 ataaggtggg ggtcttatgt agttttgtat ctgttttgca gcagccgccg ccgccatgag
     2461 caccaactcg tttgatggaa gcattgtgag ctcatatttg acaacgcgca tgcccccatg
     2521 ggccggggtg cgtcagaatg tgatgggctc cagcattgat ggtcgccccg tcctgcccgc
     2581 aaactctact accttgacct acgagaccgt gtctggaacg ccgttggaga ctgcagcctc
     2641 cgccgccgct tcagccgctg cagccaccgc ccgcgggatt gtgactgact ttgctttcct
     2701 gagcccgctt gcaagcagtg cagcttcccg ttcatccgcc cgcgatgaca agttgacggc
     2761 tcttttggca caattggatt ctttgacccg ggaacttaat gtcgtttctc agcagctgtt
     2821 ggatctgcgc cagcaggttt ctgccctgaa ggcttcctcc cctcccaatg cggtttaaaa
     2881 cataaataaa aaaccagact ctgtttggat ttggatcaag caagtgtctt gctgtcttta
     2941 tttaggggtt ttgcgcgcgc ggtaggcccg ggaccagcgg tctcggtcgt tgagggtcct
     3001 gtgtattttt tccaggacgt ggtaaaggtg actctggatg ttcagataca tgggcataag
     3061 cccgtctctg gggtggaggt agcaccactg cagagcttca tgctgcgggg tggtgttgta
     3121 gatgatccag tcgtagcagg agcgctgggc gtggtgccta aaaatgtctt tcagtagcaa
     3181 gctgattgcc aggggcaggc ccttggtgta agtgtttaca aagcggttaa gctgggatgg
     3241 gtgcatacgt ggggatatga gatgcatctt ggactgtatt tttaggttgg ctatgttccc
     3301 agccatatcc ctccggggat tcatgttgtg cagaaccacc agcacagtgt atccggtgca
     3361 cttgggaaat ttgtcatgta gcttagaagg aaatgcgtgg aagaacttgg agacgccctt
     3421 gtgacctcca agattttcca tgcattcgtc cataatgatg gcaatgggcc cacgggcggc
     3481 ggcctgggcg aagatatttc tgggatcact aacgtcatag ttgtgttcca ggatgagatc
     3541 gtcataggcc atttttacaa agcgcgggcg gagggtgcca gactgcggta taatggttcc
     3601 atccggccca ggggcgtagt taccctcaca gatttgcatt tcccacgctt tgagttcaga
     3661 tggggggatc atgtctacct gcggggcgat gaagaaaacg gtttccgggg taggggagat
     3721 cagctgggaa gaaagcaggt tcctgagcag ctgcgactta ccgcagccgg tgggcccgta
     3781 aatcacacct attaccggct gcaactggta gttaagagag ctgcagctgc cgtcatccct
     3841 gagcaggggg gccacttcgt taagcatgtc cctgactcgc atgttttccc tgaccaaatc
     3901 cgccagaagg cgctcgccgc ccagcgatag cagttcttgc aaggaagcaa agtttttcaa
     3961 cggtttgaga ccgtccgccg taggcatgct tttgagcgtt tgaccaagca gttccaggcg
     4021 gtcccacagc tcggtcacct gctctacggc atctcgatcc agcatatctc ctcgtttcgc
     4081 gggttggggc ggctttcgct gtacggcagt agtcggtgct cgtccagacg ggccagggtc
     4141 atgtctttcc acgggcgcag ggtcctcgtc agcgtagtct gggtcacggt gaaggggtgc
     4201 gctccgggct gcgcgctggc cagggtgcgc ttgaggctgg tcctgctggt gctgaagcgc
     4261 tgccggtctt cgccctgcgc gtcggccagg tagcatttga ccatggtgtc atagtccagc
     4321 ccctccgcgg cgtggccctt ggcgcgcagc ttgcccttgg aggaggcgcc gcacgagggg
     4381 cagtgcagac ttttgagggc gtagagcttg ggcgcgagaa ataccgattc cggggagtag
     4441 gcatccgcgc cgcaggcccc gcagacggtc tcgcattcca cgagccaggt gagctctggc
     4501 cgttcggggt caaaaaccag gtttccccca tgctttttga tgcgtttctt acctctggtt
     4561 tccatgagcc ggtgtccacg ctcggtgacg aaaaggctgt ccgtgtcccc gtatacagac
     4621 ttgagaggga gtttaaacga attcaatagc ttgttgcatg ggcggcgata taaaatgcaa
     4681 ggtgctgctc aaaaaatcag gcaaagcctc gcgcaaaaaa gaaagcacat cgtagtcatg
     4741 ctcatgcaga taaaggcagg taagctccgg aaccaccaca gaaaaagaca ccatttttct
     4801 ctcaaacatg tctgcgggtt tctgcataaa cacaaaataa aataacaaaa aaacatttaa
     4861 acattagaag cctgtcttac aacaggaaaa acaaccctta taagcataag acggactacg
     4921 gccatgccgg cgtgaccgta aaaaaactgg tcaccgtgat taaaaagcac caccgacagc
     4981 tcctcggtca tgtccggagt cataatgtaa gactcggtaa acacatcagg ttgattcaca
     5041 tcggtcagtg ctaaaaagcg accgaaatag cccgggggaa tacatacccg caggcgtaga
     5101 gacaacatta cagcccccat aggaggtata acaaaattaa taggagagaa aaacacataa
     5161 acacctgaaa aaccctcctg cctaggcaaa atagcaccct cccgctccag aacaacatac
     5221 agcgcttcca cagcggcagc cataacagtc agccttacca gtaaaaaaga aaacctatta
     5281 aaaaaacacc actcgacacg gcaccagctc aatcagtcac agtgtaaaaa agggccaagt
     5341 gcagagcgag tatatatagg actaaaaaat gacgtaacgg ttaaagtcca caaaaaacac
     5401 ccagaaaacc gcacgcgaac ctacgcccag aaacgaaagc caaaaaaccc acaacttcct
     5461 caaatcgtca cttccgtttt cccacgttac gtcacttccc attttaagaa aactacaatt
     5521 cccaacacat acaagttact ccgccctaaa acctacgtca cccgccccgt tcccacgccc
     5581 cgcgccacgt cacaaactcc accccctcat tatcatattg gcttcaatcc aaaataaggt
     5641 atattattga tgatgttaat taacatgcat ggatccatat gcggtgtgaa ataccgcaca
     5701 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     5761 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     5821 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5881 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5941 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6001 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6061 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6121 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6181 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6241 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6301 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     6361 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6421 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6481 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6541 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6601 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     6661 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     6721 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     6781 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     6841 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     6901 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6961 atagtttgcg caacgttgtt gccattgctg cagccatgag attatcaaaa aggatcttca
     7021 cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
     7081 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
     7141 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
     7201 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
     7261 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat
     7321 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     7381 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     7441 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     7501 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     7561 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     7621 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     7681 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     7741 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     7801 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     7861 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     7921 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     7981 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     8041 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     8101 tctatcgcct tcttgacgag ttcttctgaa ttttgttaaa atttttgtta aatca
//
