LOCUS       62056_2900       11123 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(523..556)
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     CDS             1344..5447
                     /codon_start=1
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5484..5504
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             5508..5528
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             5529..5594
                     /codon_start=1
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      5659..5906
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             6465..7733
                     /codon_start=1
                     /label=FLP
                     /note="site-specific recombinase"
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     promoter        8164..8507
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        complement(9015..9045)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9060..9081)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9369..9957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10131..10988)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10989..11093)
                     /label=AmpR promoter
ORIGIN
        1 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
       61 tgacggcacg gccacgcgtt taaaccgcct caagccatgg gatatgctca aaatattgct
      121 taactggggg tttaacaaat acatgtatgg taatccaaat gggtatgggg ttagtagtaa
      181 tttggtagtg aaaaccccgg tagacggaga attaataaat accccaaaat ccaccacagg
      241 actcgacagg tacctggagg atgaggagac agaagttagt agtaacaatt tggggacaga
      301 agaggataca gtgacatcag aggaagaaga cagcgaaggt atagtcaaaa agcaatagga
      361 cagcaagaca atttatttat ttaatagttt tttataaatg taaaatctat cacaacatgt
      421 gtacttaatt ttctgtggta ggggaggcca tttcgttgaa acgtgtcgcg agcggttgtg
      481 agaattgact cattttgatt attacgcaac cattgaaatt ttgaagttcc tatactttct
      541 agagaatagg aacttcacag ctaacaatta cacaaaaaaa attctctttt cgatgtagca
      601 actatcttca actaaaacat tttctccttc ggcccatgat tgtcctccgg gtcgacagca
      661 agccgttaca attgagatgg aaagcgacct accttcactc gataaggtgc ttaattgtac
      721 ttcatataaa tctggcccgg atctaaacaa atgagttcca ttaagccgtg ggttctcaat
      781 tagggttttt gtttttgatt tagaaaaaag agatcaagat ttgtttacag gtgatgcctt
      841 tttttagaac ttatgcgttg caaaagttga ctaacgattt ctataaggtg atccacacta
      901 attatacaaa cgtacaaaca gacatacttt tcctgcgttc acctgatgtt ggccagattt
      961 ctctcttcat tgcatagaac ataaccacac tagggcaaca gaaaaaaaaa aaaaaaaagt
     1021 gcatcgggaa gttgtgttcc attcattata tgtctactac tgcatatgag tagcccaccc
     1081 accaccacca tagtaagttt ttgtgtatgc gcgccgtcag gttatttcat ttctgaattt
     1141 ttcaaccacc ttactccctt tattgttgat tgacaatttt gctcacagta agatctttta
     1201 gactccaatt aatataaaat aagtctgatt ttccaattcc tgttttttct ttttttttct
     1261 gtttctattt ctttcctttt ctcccttttt tttaattctt cattcaatca tcaattgata
     1321 attcaggaat attacaaccc gggatggata aaaagtatag tattggttta gatattggta
     1381 ctaactctgt gggttgggca gttatcactg acgaatataa agttccatca aagaaattta
     1441 aggtgttagg taacactgac agacactcaa taaaaaagaa tcttatcggt gctcttttgt
     1501 tcgactccgg tgaaactgcc gaggctacac gtttaaaaag aacagcaaga agaagatata
     1561 cccgtagaaa aaatagaata tgttatttac aagaaatctt ttctaatgaa atggctaaag
     1621 ttgatgattc ctttttccat agattggaag agtcattttt ggttgaagaa gacaaaaagc
     1681 atgagagaca tccaatcttt gggaatatag ttgatgaagt ggcttaccat gaaaaatatc
     1741 ctaccattta tcatttaaga aagaaattgg tagattcaac tgataaagct gaccttagat
     1801 taatctattt agcacttgcc catatgatta aatttagagg tcattttttg attgaaggtg
     1861 atttgaaccc agataattct gacgtggata aattatttat tcaattagtc caaacctaca
     1921 accaattatt tgaggaaaat ccaattaatg ctagtggtgt cgatgccaaa gctatattat
     1981 cagccagatt atcaaaatct agacgtttgg aaaatttgat tgcccaattg ccaggagaaa
     2041 aaaagaatgg attatttgga aacttgatcg cattatcatt gggtttgaca ccaaatttta
     2101 aatctaattt tgatttagct gaagatgcta aattacaatt atcaaaagac acctatgacg
     2161 acgatttgga caatttactt gctcaaattg gtgatcaata tgcagatttg ttcttagctg
     2221 ctaaaaactt atctgatgct attttgttgt ctgatatttt gagagtgaac acagaaataa
     2281 ccaaagctcc attatcagca tctatgatca aacgttatga tgaacaccat caggatttga
     2341 ctttattgaa agctttggtg agacaacaat tgccagagaa gtataaagaa atctttttcg
     2401 atcaatctaa aaacgggtat gcaggttata ttgatggggg tgcctcccaa gaggaatttt
     2461 acaaatttat aaaacctatt ttagaaaaga tggatgggac tgaggaactt ttggtcaaat
     2521 tgaacagaga agatttgtta cgtaaacaga gaacttttga taatggtagt atacctcacc
     2581 aaattcattt gggtgagttg catgcaattt taagaagaca agaagatttt tatccatttt
     2641 taaaagataa tagagaaaaa atcgagaaaa ttttaacctt tagaattcca tactatgttg
     2701 ggcctttggc tagaggtaat tcaagatttg cctggatgac acgtaaatca gaagaaacta
     2761 ttaccccttg gaattttgaa gaggttgttg ataaaggagc atcagcacag agttttattg
     2821 aaagaatgac caatttcgat aaaaacttac caaatgaaaa agttttacca aaacattcct
     2881 tgttatacga atattttact gtttacaatg aacttacaaa ggttaaatat gttactgaag
     2941 gtatgcgtaa gccagccttt ttatctggag aacagaaaaa ggcaatagtt gatttattgt
     3001 ttaaaacaaa tagaaaagtt actgttaaac aattaaaaga agattacttt aagaaaattg
     3061 aatgttttga ttcagttgaa atcagtggtg ttgaagacag atttaatgct agtttaggaa
     3121 cttaccatga tttacttaaa attatcaaag ataaagattt cttggataac gaagaaaatg
     3181 aagacatttt agaagacatt gttttaacct taactttatt cgaagataga gagatgattg
     3241 aagaacgttt gaagacttat gcacatttgt ttgacgataa agtgatgaaa cagttgaaaa
     3301 gaagacgtta tactggatgg ggtagattgt ctcgtaaatt gatcaatgga attagagata
     3361 aacaaagtgg taaaactatc ttggactttt tgaaatctga cggatttgct aatagaaatt
     3421 tcatgcaatt gatccacgac gatagtttga catttaaaga agacatccaa aaggcccaag
     3481 tgagtgggca aggtgattca ttacatgaac atattgcaaa tttagccgga tctcctgcta
     3541 ttaagaaagg gatattacaa actgttaaag ttgtggatga attagtgaaa gtaatgggaa
     3601 gacataaacc tgaaaacatt gtcattgaga tggcaagaga aaatcaaact acacaaaaag
     3661 gacagaaaaa tagtagagaa cgtatgaaaa gaatagaaga gggtattaaa gaattgggta
     3721 gtcaaatatt gaaagaacac ccagtggaaa atacccagtt gcaaaatgaa aaattatatc
     3781 tttactacct tcaaaatgga cgtgatatgt atgttgatca ggaattagat ataaatagac
     3841 tttcagatta tgatgtagat catatagttc cacaatcttt cttgaaagat gattccatag
     3901 acaataaagt attaactaga agtgataaaa atagaggtaa aagtgataat gtcccaagtg
     3961 aggaagtcgt caaaaagatg aaaaattact ggcgtcaact tttgaatgct aaattaatta
     4021 ctcaaagaaa atttgataat ttgactaaag cagaaagagg tgggctttct gaattagata
     4081 aagccgggtt cattaaaaga caattggtcg aaactagaca aattactaaa catgttgccc
     4141 aaattttaga ttcccgtatg aacactaagt atgacgaaaa tgataagtta atacgtgagg
     4201 ttaaagtcat tactttaaaa tcaaaacttg tctctgattt cagaaaggat ttccaattct
     4261 ataaagttag agaaattaat aattatcatc atgctcatga tgcatatttg aatgctgtag
     4321 ttggaactgc tttaatcaag aaatacccta aattagaatc tgaatttgta tatggtgatt
     4381 acaaagtcta tgatgttaga aagatgattg ctaaatcaga acaagaaatt ggtaaagcta
     4441 cagctaaata cttcttttac tctaacatta tgaatttctt taaaacagaa attactttgg
     4501 caaacggtga aattagaaaa agacctctta ttgaaacaaa tggtgagact ggagagatag
     4561 tttgggacaa agggcgtgat ttcgctactg ttagaaaagt tttatcaatg ccacaagtta
     4621 acattgtaaa gaaaacagag gttcaaactg gtggtttctc aaaagaaagt attttgccta
     4681 aaagaaatag tgataaattg attgccagaa aaaaggattg ggatccaaag aaatatggtg
     4741 gtttcgactc accaaccgta gcctattctg ttttggttgt ggcaaaggtt gaaaagggta
     4801 aaagtaaaaa gcttaaatca gtaaaagaac ttttgggtat tacaataatg gaaagaagtt
     4861 cctttgaaaa gaaccctatt gattttttgg aagctaaagg ttataaggaa gtaaagaagg
     4921 acttaataat caaattgcct aaatattctt tatttgaatt agaaaatggg agaaaaagaa
     4981 tgttggcttc tgctggagaa ttgcaaaagg gtaatgaatt agcattgcct tccaaatatg
     5041 ttaacttctt gtatttagct tcacactatg aaaagttgaa agggtcacca gaagataacg
     5101 agcaaaaaca attatttgtt gaacaacaca aacactactt agatgagatt atagaacaaa
     5161 ttagtgaatt cagtaaaaga gtgatattag ctgatgcaaa tttagataaa gttttgtcag
     5221 cctataacaa acatagagat aagccaatta gagaacaagc agaaaacatt attcacttat
     5281 ttacccttac caatttagga gcacctgctg ctttcaagta ttttgataca acaattgatc
     5341 gtaaaagata tacctcaaca aaagaagtct tagacgccac cttaattcat caatcaatca
     5401 ctggattgta tgagacaaga attgatttgt ctcaattggg tggtgatgaa ggggctgatc
     5461 ctaagaagaa aagaaaagtt gatccaaaga aaaagcgtaa ggtggatcct aagaaaaaga
     5521 gaaaggttga ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg
     5581 atgacgatga caagtgataa tgactgcaga gatccatcga cctgccgcca agctaattcc
     5641 gggcgaattt ctgtcgagtc atgtaattag ttatgtcacg cttacattca cgccctcccc
     5701 ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt
     5761 atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt
     5821 ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt
     5881 tgggacgctc gaaggcttta atttgcggcc ccaataatga ttggtttgat atttttgtct
     5941 agtaccatct gtaccattac acttaaatta tctttatatc tgtctaactc gactgtctgg
     6001 atttcattga tgtagtcgta tgcatcgtta gttccaaaaa atattgtcat caatttgata
     6061 ttggtttccg actctaaaat ttttggaaga atttgtctag cgtgctctga gttgtagcca
     6121 ctgaaaccac ggttaataac atccaatttt cggatataca cattctgtaa tgctggatga
     6181 aagccatact gggtacaact aaactgggtg atggagtcac cgaacaacac aaatttaccg
     6241 tattccatga ttgctatggt tgagaatttt ttttttttct tgtcccacgc catttttcaa
     6301 attatgcagt tgagaatgtt agtttttgtg tacaccccgt tcgctgaata tttcggaata
     6361 attcaaagat tggggagtgg gggaggcgat agacgaagac acggtataaa aatgggcaaa
     6421 attttcccca actttttgca gtggtttaac taataatcgt cgacatgcca caatttgata
     6481 tattatgtaa aacaccacct aaggtgcttg ttcgtcagtt tgtggaaagg tttgaaagac
     6541 cttcaggtga gaaaatagca ttatgtgctg ctgaactaac ctatttatgt tggatgatta
     6601 cacataacgg aacagcaatc aagagagcca cattcatgag ctataatact atcataagca
     6661 attcgttgag tttcgatatt gtcaataaat cactccagtt taaatacaag acgcaaaaag
     6721 caacaatttt ggaagcctca ttaaagaaat tgattcctgc ttgggaattt acaattattc
     6781 cttactatgg acaaaaacat caatctgata tcactgatat tgtaagtagt ttgcaattac
     6841 agttcgaatc atcggaagaa gcagataagg gaaatagcca cagtaaaaaa atgcttaaag
     6901 cacttctaag tgagggtgaa agcatctggg agatcactga gaaaatacta aattcgtttg
     6961 agtatacttc gagatttaca aaaacaaaaa ctttatacca attcctcttc ctagctactt
     7021 tcatcaattg tggaagattc agcgatatta agaacgttga tccgaaatca tttaaattag
     7081 tccaaaataa gtatttggga gtaataatcc agtgtttagt gacagagaca aagacaagcg
     7141 ttagtaggca catatacttc tttagcgcaa ggggtaggat cgatccactt gtatatttgg
     7201 atgaattttt gaggaattct gaaccagtcc taaaacgagt aaataggacc ggcaattctt
     7261 caagcaataa acaggaatac caattattaa aagataactt agtcagatcg tacaataaag
     7321 ctttgaagaa aaatgcgcct tattcaatct ttgctataaa aaatggccca aaatctcaca
     7381 ttggaagaca tttgatgacc tcatttcttt caatgaaggg cctaacggag ttgactaatg
     7441 ttgtgggaaa ttggagcgat aagcgtgctt ctgccgtggc caggacaacg tatactcatc
     7501 agataacagc aatacctgat cactacttcg cactagtttc tcggtactat gcatatgatc
     7561 caatatcaaa ggaaatgata gcattgaagg atgagactaa tccaattgag gagtggcagc
     7621 atatagaaca gctaaagggt agtgctgaag gaagcatacg ataccccgca tggaatggga
     7681 taatatcaca ggaggtacta gactaccttt catcctacat aaatagacgc atataagagt
     7741 gaaattctgg aaatctggaa atctggtttt gtattcttgt tattcttctt tttgttatta
     7801 catatataac ttgttacttt tttaaaaaaa tctttgtata ttttataaat atataaaact
     7861 aaatttaaga aaaagagaaa aatgttttat ttgagagatt gaaattttac ttgaatttag
     7921 cttagctttt ataaagtatt attatgtaaa aaaacaaaac aaatatacat taaaaagtta
     7981 agactataaa atagccaccc aaggcatttc tatatcttgt tgttgttgtt ttcatcttct
     8041 gtatcagagg aacttatttt attattttcg tcacgggtat tttctcttgt ttgatgattc
     8101 atcccattca ttccatcata aaatatcaag cttgcctcgt ccccgccggg tcacccggcc
     8161 agcgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca
     8221 tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc
     8281 atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc
     8341 gagcagggaa acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag
     8401 agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa
     8461 atcttgctag gatacagttc tcacatcaca tccgaacata aacaaccgga tcctacaatt
     8521 atgtctacta ctttggatga tactgcttat agatacagaa cttctgttcc aggtgatgct
     8581 gaagctattg aagctttgga tggttctttc actaccgata ctgtttttag agttactgct
     8641 actggtgatg gtttcacttt gagagaagtt ccagttgatc caccattgac taaggttttc
     8701 ccagatgatg aatccgatga tgaatccgat gctggtgaag atggtgatcc agattctaga
     8761 actttcgttg cttatggtga tgatggtgat ttggctggtt tcgttgttgt ttcttattct
     8821 ggttggaaca gaagattgac tgttgaagat attgaagttg ctccagaaca tagaggtcat
     8881 ggtgttggta gagctttgat gggtttggct actgaattcg ccagagaaag aggtgctggt
     8941 catttgtggt tggaagttac caatgttaat cggtgtttaa accccagcgc ctggcgggcg
     9001 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     9061 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     9121 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     9181 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     9241 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     9301 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     9361 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     9421 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     9481 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     9541 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     9601 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     9661 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9721 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9781 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9841 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9901 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9961 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    10021 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    10081 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgcttg
    10141 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    10201 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    10261 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    10321 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    10381 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    10441 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    10501 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    10561 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10621 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10681 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10741 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10801 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10861 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10921 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10981 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    11041 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    11101 ccccgaaaag tgccacctga cgt
//
