LOCUS       40924_4065        8250 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pADH77, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Nobile CJ.
  TITLE     The Transcriptional Circuitry Controlling Candida albicans Biofilm 
            Formation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8250)
  AUTHORS   Hernday AD.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2011) Biochemistry and Biophysics, UCSF, Genentech
            Hall N374, 600 16th Street, San Francisco, CA 94143, USA
REFERENCE   3  (bases 1 to 8250)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8250)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-SEP-2011) Biochemistry and Biophysics, UCSF, Genentech Hall 
            N374, 600 16th Street, San Francisco, CA 94143, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             35..742
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     protein_bind    complement(752..785)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     CDS             1344..2612
                     /codon_start=1
                     /label=FLP
                     /note="site-specific recombinase"
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     protein_bind    complement(4872..4905)
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     primer_bind     complement(4907..4923)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4960..4978)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4999..5015)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5023..5039)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5047..5077)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5092..5113)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5401..5989)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        6209..6311
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             6312..6968
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(7459..7564)
                     /label=AmpR promoter
     rep_origin      7591..8046
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8187..8203
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8213..8231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccccctcgag cggatccccg ggttaattaa cggtatggtt tctaagggtg aagaagataa
       61 catggctatt atcaaagaat ttatgagatt caaagtccac atggaaggtt ctgttaacgg
      121 tcacgaattc gaaattgaag gtgaaggtga aggtagacca tacgaaggta ctcaaaccgc
      181 taagttgaaa gttactaagg gtggtccatt gccattcgcc tgggatattt tgtctccaca
      241 attcatgtac ggttctaagg cttacgttaa acatccagct gatatcccag actacttgaa
      301 attatctttc ccagaaggtt ttaaatggga aagagttatg aacttcgaag atggtggtgt
      361 tgtcaccgtt actcaagact cttccttgca agacggtgaa ttcatttata aagttaagtt
      421 gagaggtact aatttcccat ctgatggtcc agttatgcaa aagaaaacta tgggttggga
      481 agcctcttcc gaaagaatgt acccagaaga cggtgctttg aaaggtgaaa ttaagcaaag
      541 attgaaatta aaggatggtg gtcactacga cgctgaagtc aaaactacct acaaggctaa
      601 gaaaccagtt caattaccag gtgcctataa cgtcaatatc aaattggaca ttacctccca
      661 taacgaagat tacactattg ttgaacaata cgaaagagct gaaggtagac actctactgg
      721 tggtatggat gaattgtaca agtaactcga ggaagttcct atactttcta gagaatagga
      781 acttcggatc caataatgat tggtttgata tttttgtcta gtaccatctg taccattaca
      841 cttaaattat ctttatatct gtctaactcg actgtctgga tttcattgat gtagtcgtat
      901 gcatcgttag ttccaaaaaa tattgtcatc aatttgatat tggtttccga ctctaaaatt
      961 tttggaagaa tttgtctagc gtgctctgag ttgtagccac tgaaaccacg gttaataaca
     1021 tccaattttc ggatatacac attctgtaat gctggatgaa agccatactg ggtacaacta
     1081 aactgggtga tggagtcacc gaacaacaca aatttaccgt attccatgat tgctatggtt
     1141 gagaattttt tttttttctt gtcccacgcc atttttcaaa ttatgcagtt gagaatgtta
     1201 gtttttgtgt acaccccgtt cgctgaatat ttcggaataa ttcaaagatt ggggagtggg
     1261 ggaggcgata gacgaagaca cggtataaaa atgggcaaaa ttttccccaa ctttttgcag
     1321 tggtttaact aataatcgtc gacatgccac aatttgatat attatgtaaa acaccaccta
     1381 aggtgcttgt tcgtcagttt gtggaaaggt ttgaaagacc ttcaggtgag aaaatagcat
     1441 tatgtgctgc tgaactaacc tatttatgtt ggatgattac acataacgga acagcaatca
     1501 agagagccac attcatgagc tataatacta tcataagcaa ttcgttgagt ttcgatattg
     1561 tcaataaatc actccagttt aaatacaaga cgcaaaaagc aacaattttg gaagcctcat
     1621 taaagaaatt gattcctgct tgggaattta caattattcc ttactatgga caaaaacatc
     1681 aatctgatat cactgatatt gtaagtagtt tgcaattaca gttcgaatca tcggaagaag
     1741 cagataaggg aaatagccac agtaaaaaaa tgcttaaagc acttctaagt gagggtgaaa
     1801 gcatctggga gatcactgag aaaatactaa attcgtttga gtatacttcg agatttacaa
     1861 aaacaaaaac tttataccaa ttcctcttcc tagctacttt catcaattgt ggaagattca
     1921 gcgatattaa gaacgttgat ccgaaatcat ttaaattagt ccaaaataag tatttgggag
     1981 taataatcca gtgtttagtg acagagacaa agacaagcgt tagtaggcac atatacttct
     2041 ttagcgcaag gggtaggatc gatccacttg tatatttgga tgaatttttg aggaattctg
     2101 aaccagtcct aaaacgagta aataggaccg gcaattcttc aagcaataaa caggaatacc
     2161 aattattaaa agataactta gtcagatcgt acaataaagc tttgaagaaa aatgcgcctt
     2221 attcaatctt tgctataaaa aatggcccaa aatctcacat tggaagacat ttgatgacct
     2281 catttctttc aatgaagggc ctaacggagt tgactaatgt tgtgggaaat tggagcgata
     2341 agcgtgcttc tgccgtggcc aggacaacgt atactcatca gataacagca atacctgatc
     2401 actacttcgc actagtttct cggtactatg catatgatcc aatatcaaag gaaatgatag
     2461 cattgaagga tgagactaat ccaattgagg agtggcagca tatagaacag ctaaagggta
     2521 gtgctgaagg aagcatacga taccccgcat ggaatgggat aatatcacag gaggtactag
     2581 actacctttc atcctacata aatagacgca tataagagtg aaattctgga aatctggaaa
     2641 tctggttttg tattcttgtt attcttcttt ttgttattac atatataact tgttactttt
     2701 ttaaaaaaat ctttgtatat tttataaata tataaaacta aatttaagaa aaagagaaaa
     2761 atgttttatt tgagagattg aaattttact tgaatttagc ttagctttta taaagtatta
     2821 ttatgtaaaa aaacaaaaca aatatacatt aaaaagttaa gactataaaa tagccaccca
     2881 aggcatttct atatcttgtt gttgttgttt tcatcttctg tatcagagga acttatttta
     2941 ttattttcgt cacgggtatt ttctcttgtt tgatgattca tcccattcat tccatcataa
     3001 aatgtcgagc gtcaaaacta gagaataata aagaaaacga tcttttcaaa aagaaaaaac
     3061 cttttagttt tcctttgttg ttgttgtggg tgtgtgctat ttatattata tagtttactc
     3121 ataataccat aaaatattcg gtttgattag gttattttaa taagctaatt tgtttctaat
     3181 cgtgtaattt atgctgtgta tattaagtag tgtgtgcact gcccaaaaat gtttgttgtt
     3241 tatagtcggt taaagagaaa aaagaaaaaa agatccatac acacacgtta attagttgtt
     3301 caacgtaata cactcatatt ttgttcttat ttgctttcgg tcgctgttct caccaagatt
     3361 tattgccaac gaaacaattt ttttttatat attttcagat ttttcttttt ttcctttcct
     3421 ttccttttct aattttcact cctggttttc tttctttctt agaaacatta tctcgatatt
     3481 aatattaaaa aaatataatc attcaaaatg gacggtggta tgttttagtt tagcttcaat
     3541 tctaattgat tgattaatca gttgattggt ttcaatatga caaatgggta gggtgggaaa
     3601 acttcatttt caattcagat caaacttttt tgttgtcgac ataatatttc tcgtttggga
     3661 tgttactgtc acattaataa tacacacaca tcagcttata attttgaaag taatttatca
     3721 gatatgttgt gacgatcaat ggaaatggct aacttcaatg tatctgttct tccccttttt
     3781 caaagttcac gttttttgat tgattgattg atctgtcggc agtggtttca aaaccattcg
     3841 gtgagtaatc ctatcaatca atgttacgac aaaaggctca atattcaaaa ttgcaatgtt
     3901 ttatgttttc ctacgtgtac ttgtgcaagg caattgattc aacattgctt ttggtgtttg
     3961 acgagtttct agtttggact tgtgttgtta tctgggctat acagatttcc cggctcacta
     4021 tgaatttttt ttttcgacgc tcagtgcaca caactataaa caacacaaac acaaacacag
     4081 caagaaaaaa aaaaaacgaa cattgaattg aaaccaagcc aactgaaaaa ttccttattt
     4141 aaatgactgt catactaacc catttttata gaagaagttg ctgctttagt tatcgataac
     4201 ggttctcata tgaaaatttc ggtgatccct gagcaggtgg cggaaacatt ggatgctgag
     4261 aaccatttca ttgttcgtga agtgttcgat gtgcacctat ccgaccaagg ctttgaacta
     4321 tctaccagaa gtgtgagccc ctaccggaag gattacatct cggatgatga ctctgatgaa
     4381 gactctgctt gctatggcgc attcatcgac caagagcttg tcgggaagat tgaactcaac
     4441 tcaacatgga acgatctagc ctctatcgaa cacattgttg tgtcgcacac gcaccgaggc
     4501 aaaggagtcg cgcacagtct catcgaattt gcgaaaaagt gggcactaag cagacagctc
     4561 cttggcatac gattagagac acaaacgaac aatgtacctg cctgcaattt gtacgcaaaa
     4621 tgtggcttta ctctcggcgg cattgacctc ttcacgtata aaactagacc tcaagtctcg
     4681 aacgaaacag cgatgtactg gtactggttc tcgggagcac aggatgacgc ctaacatatg
     4741 tgaagtgtga agggggagat tttcacttta ttagatttgt atatatgtat aataaataaa
     4801 taaataagtt aaataaataa ttagataagg gtggtaatta ttactattta caatcaaagg
     4861 tggtcctgca ggaagttcct atactttcta gagaatagga acttcagatc cactagttct
     4921 agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
     4981 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5041 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     5101 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     5161 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     5221 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5281 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5341 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5401 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5461 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5521 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5581 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     5641 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5701 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5761 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5821 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     5881 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5941 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6001 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6061 catgagatta tcaaaaagga tcttcaccta gatccttttc gaccgaataa atacctgtga
     6121 cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc gggaagccct
     6181 gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc
     6241 ataatgaaat aagatcacta ccgggcgtat tttttgagtt gtcgagattt tcaggagcta
     6301 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     6361 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     6421 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     6481 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaatta cgtatggcaa
     6541 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     6601 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     6661 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     6721 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     6781 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     6841 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     6901 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     6961 gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt tgctacgcct
     7021 gaataagtga taataagcgg atgaatggca gaaattcgaa agcaaattcg acccggtcgt
     7081 cggttcaggg cagggtcgtt aaatagccgc ttatgtctat tgctggttta ccggtttatt
     7141 gactaccgga agcagtgtga ccgtgtgctt ctcaaatgcc tgaggccagt ttgctcaggc
     7201 tctccccgtg gaggtaataa ttgacgatat gatccttttt ttctgatcaa aagtgctcat
     7261 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7321 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7381 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     7441 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcaagggtt
     7501 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     7561 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
     7621 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     7681 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     7741 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     7801 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     7861 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     7921 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     7981 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     8041 caatttccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     8101 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     8161 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
     8221 ctcactatag ggcgaattgg gtaccgggcc
//
