LOCUS       40924_4055        7117 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pADH33, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Hernday A.
  TITLE     Structure of the Transcriptional Network Controlling White-Opaque 
            Switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7117)
  AUTHORS   Hernday A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-NOV-2012) Microbiology and Immunology, UCSF, 600 16th 
            St, San Francisco, CA 94107, USA
REFERENCE   3  (bases 1 to 7117)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7117)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-NOV-2012) Microbiology and Immunology, UCSF, 600 16th St, San 
            Francisco, CA 94107, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..989
                     /gene="pMET3"
                     /regulatory_class="promoter"
     gene            1..989
                     /gene="pMET3"
                     /label=pMET3
     primer_bind     complement(1035..1051)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1059..1075)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1083..1113)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1128..1149)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    1776..3500
                     /label=SAT1 marker
                     /note="SAT1 marker"
     rep_origin      complement(3697..4285)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4459..5316)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5317..5421)
                     /label=AmpR promoter
     misc_feature    complement(5699..6544)
                     /label=RP10 integration sequence
                     /note="RP10 integration sequence"
     primer_bind     6750..6766
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cattcccttg ggaattatac tgagtaatgg gaagaatgtg caaaaaatac ccgtgtctta
       61 tataatttcc atttgtgtag ggcttaaaag tggacaactt caggttacga aaccacgaaa
      121 atgtctaaac gtttgatcaa attcgtaact catggaaaat cacagcaaca aattaaggat
      181 ggatatatcc ggtacttaca aataaaaaca ctaaagaagt ataaattttg aactgaaatt
      241 ctatagaaaa tctttttttt ttgggtaaaa ttatgcgatt gtggctcata gtaacgtaat
      301 catacagttc aacaaacatc attttgtcaa aggatttcta atcccattct ttttctactt
      361 ggcttttgag tatatatata tatacatcct ttgttcatca tcatgttcga ttgaattttt
      421 ttcttggttg ttattagatt gtctacttga gtttttcttg gtgcaaattg tggtgttttt
      481 ctaacaaatt tttcaacatt taaagtattt ctggaacttt tctacaagga acgacataga
      541 tccattaatg cgcccctcta aaacaatacc cataatgtct tcttaagtat gcatctgctt
      601 ataaatgttt ctggatttac tttagttaga atgtcgacac gacaatgaac tattgctgtg
      661 gatcacgtgc gatcactgaa ttatgagttt ctttttattt ttctttttgg gctttttttt
      721 gcaatttttt ttttccacct ttgctaattg cgtagtcaca tataatgtat acaatttttg
      781 caactctgac actataattg atccaataat aaagtatata atagatcatc aagtatacgt
      841 aatctcccca aaaaaaaaat tgcataaaat aaaatctata aacctttttt agatttttag
      901 ttgttccttt tttctttcta ttcttcaact tccaagtgtt gtcactttct tcctgttaac
      961 tatatttaaa agtaaatacc ctccccggat cccccgggct gcaggaattc gatatcaagc
     1021 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     1081 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     1141 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     1201 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     1261 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     1321 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1381 tgagtgaaat tctggaaatc tggaaatctg gttttgtatt cttgttattc ttctttttgt
     1441 tattacatat ataacttgtt acttttttaa aaaaatcttt gtatatttta taaatatata
     1501 aaactaaatt taagaaaaag agaaaaatgt tttatttgag agattgaaat tttacttgaa
     1561 tttagcttag cttttataaa gtattattat gtaaaaaaac aaaacaaata tacattaaaa
     1621 agttaagact ataaaatagc cacccaaggc atttctatat cttgttgttg ttgttttcat
     1681 cttctgtatc agaggaactt attttattat tttcgtcacg ggtattttct cttgtttgat
     1741 gattcatccc attcattcca tcataaaatg tcgagcgtca aaactagaga ataataaaga
     1801 aaacgatctt ttcaaaaaga aaaaaccttt tagttttcct ttgttgttgt tgtgggtgtg
     1861 tgctatttat attatatagt ttactcataa taccataaaa tattcggttt gattaggtta
     1921 ttttaataag ctaatttgtt tctaatcgtg taatttatgc tgtgtatatt aagtagtgtg
     1981 tgcactgccc aaaaatgttt gttgtttata gtcggttaaa gagaaaaaag aaaaaaagat
     2041 ccatacacac acgttaatta gttgttcaac gtaatacact catattttgt tcttatttgc
     2101 tttcggtcgc tgttctcacc aagatttatt gccaacgaaa caattttttt ttatatattt
     2161 tcagattttt ctttttttcc tttcctttcc ttttctaatt ttcactcctg gttttctttc
     2221 tttcttagaa acattatctc gatattaata ttaaaaaaat ataatcattc aaaatggacg
     2281 gtggtatgtt ttagtttagc ttcaattcta attgattgat taatcagttg attggtttca
     2341 atatgacaaa tgggtagggt gggaaaactt cattttcaat tcagatcaaa cttttttgtt
     2401 gtcgacataa tatttctcgt ttgggatgtt actgtcacat taataataca cacacatcag
     2461 cttataattt tgaaagtaat ttatcagata tgttgtgacg atcaatggaa atggctaact
     2521 tcaatgtatc tgttcttccc ctttttcaaa gttcacgttt tttgattgat tgattgatct
     2581 gtcggcagtg gtttcaaaac cattcggtga gtaatcctat caatcaatgt tacgacaaaa
     2641 ggctcaatat tcaaaattgc aatgttttat gttttcctac gtgtacttgt gcaaggcaat
     2701 tgattcaaca ttgcttttgg tgtttgacga gtttctagtt tggacttgtg ttgttatctg
     2761 ggctatacag atttcccggc tcactatgaa tttttttttt cgacgctcag tgcacacaac
     2821 tataaacaac acaaacacaa acacagcaag aaaaaaaaaa aacgaacatt gaattgaaac
     2881 caagccaact gaaaaattcc ttatttaaat gactgtcata ctaacccatt tttatagaag
     2941 aagttgctgc tttagttatc gataacggtt ctcatatgaa aatttcggtg atccctgagc
     3001 aggtggcgga aacattggat gctgagaacc atttcattgt tcgtgaagtg ttcgatgtgc
     3061 acctatccga ccaaggcttt gaactatcta ccagaagtgt gagcccctac cggaaggatt
     3121 acatctcgga tgatgactct gatgaagact ctgcttgcta tggcgcattc atcgaccaag
     3181 agcttgtcgg gaagattgaa ctcaactcaa catggaacga tctagcctct atcgaacaca
     3241 ttgttgtgtc gcacacgcac cgaggcaaag gagtcgcgca cagtctcatc gaatttgcga
     3301 aaaagtgggc actaagcaga cagctccttg gcatacgatt agagacacaa acgaacaatg
     3361 tacctgcctg caatttgtac gcaaaatgtg gctttactct cggcggcatt gacctcttca
     3421 cgtataaaac tagacctcaa gtctcgaacg aaacagcgat gtactggtac tggttctcgg
     3481 gagcacagga tgacgcctaa catatgtgaa gtgtgaaggg ggagattttc actttattag
     3541 atttgtatat atgtataata aataaataaa taagttaaat aaataattag ataagggtgg
     3601 taattattac tatttacaat caaaggtggt cctgcacatg tgagcaaaag gccagcaaaa
     3661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3961 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     4141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4321 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4381 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4441 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4501 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4561 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4621 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4681 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4741 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4801 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4861 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4921 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4981 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     5041 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5101 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5161 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5221 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5281 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5341 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5401 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     5461 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     5521 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     5581 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     5641 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     5701 tatgcagatc cttatcctta aacagattct aaaacaacat ctttgaaacc agaagaaact
     5761 ttcttacctt tttcttcggt tgaaccttca ccgtgcaaag ccaataatga accccagtcg
     5821 aattttggtt gtttcaacaa tttgactttt ctgatgtgga cattttgtaa tgggaaaatg
     5881 gtttgggtgg atttttcaat ttcacggcca atgacttctg gaatcaattt ggaagttaat
     5941 tgagctaaag tacagttgga aacttctctt tcgataattt caatcatctt ctttctggct
     6001 tctctcaatt tagaggattg agcgtaagta gtttccttga tttggtttgg ttgtcttttg
     6061 gtgaaagcaa tggcaaaaac tctcaaaacg taatcgtcgg aagttttaac agtgacatta
     6121 gcttcaacta atgattgcca ttttctgacc aatgatctta atttgtcaga agtgaaatcc
     6181 aaaccatgag gcctccatga ggcctccatg aggcctccat gaggcctcca tggaggcctc
     6241 atggaagttg gtcaacaagt ttttaccttg gacttcatca actctcaatt tgatttttct
     6301 gtaagagtgg tcttcggaac cttgtaagtc ggccaaacaa acttcgaaaa ctctaccttt
     6361 caagccatca gcggcattct ttaaaccggt agatctgttg atcaaagttt taccaacatt
     6421 tctgttttca aaagtggttg gagctttgat gtcaaaccaa tcttttctgg tgaatgggtc
     6481 aacgaccttc ttttttaatc ctttctttcc tttggacaat ctcttgtttt taccgacagc
     6541 catgttgtac ttgcatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     6601 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     6661 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     6721 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtctattcc
     6781 aagcctgtgg ctttttttgg tgattcacaa tacataaatt ggcaaatacc tcttctatag
     6841 actttaacct ttgatgaaat ggtatggata aaccactttt gtggtcaggg tatatatatt
     6901 tttattccaa tctggccttt ataaattcaa atttccaagg ggactctgct aaaccttctg
     6961 atataactat aactctaata atggctgtaa aaagtgtgaa aaaaagatga tattcacaaa
     7021 aaagataaag gggatttcat attgggtatc tagatttaaa catcgtattt caacaatgaa
     7081 actagaatca atacgaaatc cagtgcacaa ctgtgtt
//
