LOCUS       V012860                 5952 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V012860
VERSION     V012860
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             54..767
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     CDS             2931..3578
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    3802..3942
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4128..4716)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4890..5747)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5748..5852)
                     /label="AmpR promoter"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agatttaaga aggagatata catatgagta
       61 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
      121 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
      181 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
      241 ctttcgggta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
      301 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
      361 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
      421 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
      481 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
      541 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
      601 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
      661 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
      721 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa atctcctgca
      781 ggcatgcaag cttgagtagg acaaatccgc cgagcttcga cgagattttc aggagctaag
      841 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
      901 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
      961 cagaacaaag aatacaagaa aatatttaca aaaaatcaat ttaacaattc cttaaaacat
     1021 gcaggaattg acgatttaaa caatattagc tttgaacaat tcttatctct tttcaatagc
     1081 tataaattat ttaataagta agttaaggga tgcataaact gcatccctta acttgttttt
     1141 cgtgtgccta ttttttgtga atcgctaaga aaccattatt atcatgacat taacctataa
     1201 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     1261 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     1321 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     1381 ggcatcagag cagattgtac tgagagtgca ccatacaaaa catatttcaa cacaatacaa
     1441 atgggttagt taaaaaagca ggccttctaa aggtctgctt tttttatttg attatgtaat
     1501 ttttaatgcc aggatgccaa taagccataa cctcaaatgc accatttgca acctcgtcat
     1561 cttcttcctc aatcttgacc agatcgccgt cctccgcatc accaagattc agctctttat
     1621 gtatctcctt caaaatgcca ccgtatccaa ttaaccttcg agctgccaac gcatcatcca
     1681 agtaaagcac cgtgttcaga ttgtcttcag tcaccttatt accgcgcaca caatccgtat
     1741 ccttaaccgg atatttagag atttcgcgaa cagctttttg ctccatcatt gcgttccgca
     1801 catcgttttc aatctgttca gcgtcaatct tagctttacc tttcactcga cgaatatcga
     1861 caattggagt gtaatccaat ttcatcgcct ttttccaaag gctcgtccac tccgcctgct
     1921 taatatagtt tttcccaaaa taatttttcc ttactggtat caacacatga aaatgaggat
     1981 gatatgtatc ttcttcatga tttttggtaa tctctaaagc tctgaaaaat ccaagaaccg
     2041 aagtttttac ttttttgtac tggaacagtt tcctaaagcc ttccatcatc gcagaaattt
     2101 gtggcttcag ccgttctccc tttacatttc gaatcgtcag cgtgagaaaa atccatccgc
     2161 agccgtactg tctattggct tcctctacga tcaacttatt gtgataagca atttttaacg
     2221 acctgcgcca cgcacacatc ggacataacc tcactttaca aaaatgggct tgatacagtt
     2281 ttaacttgcc cgtctccggg tctctcttaa acgaaagata ctctgcacaa ctaattagtt
     2341 tttcagcctt tttgccatag taaggtgccc caatcttact ctctaacgct tcgtaatgct
     2401 ccgccatgag gttcgtccgt ctctttttcc ccttccaatc ccgcttttta cctgttgcgg
     2461 ttttatcttc gaggatgcta taatcatttt cagatgaata aatcaacaaa aaaactcctt
     2521 ctgagctagt tctctagcat tctattattt tgattcgaca ccttaataat agcagaagga
     2581 gtttttacct gtcaaagaac catcaaaccc ttgatacaca aggctttgac ctaattttga
     2641 aaaatgatgt tgtttctata tagtatcaag ataagaaaga aaaggatttt tcgctacgct
     2701 caaatccttt aaaaaaacac aaaagaccac attttttaat gtggtcttta ttcttcaact
     2761 aaagcaccca ttagttcaac aaacgaaaat tggataaagt gggatatttt taaaatatat
     2821 atttatgtta cagtaatatt gacttttaaa aaaggattga ttctaatgaa gaaagcagac
     2881 aagtaagcct cctaaattca ctttagataa aaatttagga ggcatatcaa atgaacttta
     2941 ataaaattga tttagacaat tggaagagaa aagagatatt taatcattat ttgaaccaac
     3001 aaacgacttt tagtataacc acagaaattg atattagtgt tttataccga aacataaaac
     3061 aagaaggata taaattttac cctgcattta ttttcttagt gacaagggtg ataaactcaa
     3121 atacagcttt tagaactggt tacaatagcg acggagagtt aggttattgg gataagttag
     3181 agccacttta tacaattttt gatggtgtat ctaaaacatt ctctggtatt tggactcctg
     3241 taaagaatga cttcaaagag ttttatgatt tatacctttc tgatgtagag aaatataatg
     3301 gttcggggaa attgtttccc aaaacaccta tacctgaaaa tgctttttct ctttctatta
     3361 ttccatggac ttcatttact gggtttaact taaatatcaa taataatagt aattaccttc
     3421 tacccattat tacagcagga aaattcatta ataaaggtaa ttcaatatat ttaccgctat
     3481 ctttacaggt acatcattct gtttgtgatg gttatcatgc aggattgttt atgaactcta
     3541 ttcaggaatt gtcagatagg cctaatgact ggcttttata atatgagata atgccgactg
     3601 tactttttac agtcggtttt ctaatgtcac taacctgccc cgttagtcgc cattcgccag
     3661 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     3721 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     3781 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     3841 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     3901 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     3961 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     4021 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     4081 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4141 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4201 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4261 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4321 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4381 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4441 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4501 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4561 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4621 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4681 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4741 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     4801 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4861 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4921 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4981 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     5041 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     5101 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     5161 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
     5221 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     5281 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     5341 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     5401 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     5461 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     5521 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     5581 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     5641 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     5701 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     5761 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     5821 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     5881 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     5941 tttcgtcttc aa
//
