LOCUS       pAD-GAL4.        7620 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Phagemid vector carrying the GAL4 activation domain for yeast 
            two-hybrid analysis.
ACCESSION   .
VERSION     .
KEYWORDS    pAD-GAL4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             422..424
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             452..472
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             488..829
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     misc_feature    833..869
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(872..890)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1277..1464
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(1585..2676)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(2677..3081)
                     /label=LEU2 promoter
     primer_bind     complement(3123..3139)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3147..3163)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3171..3201)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3216..3237)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     enhancer        complement(3405..3596)
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      3729..4184
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      complement(4450..5038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5212..6069)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6070..6174)
                     /label=AmpR promoter
     rep_origin      6456..7620
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa
      421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg
      481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt
      541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc
      601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag
      661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc
      721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata
      781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattcgc
      841 ccgggcctcg agcccgggtc gactctagag ccctatagtg agtcgtatta ctgcagagat
      901 ctatgaatcg tagatactga aaaaccccgc aagttcactt caactgtgca tcgtgcacca
      961 tctcaatttc tttcatttat acatcgtttt gccttctttt atgtaactat actcctctaa
     1021 gtttcaatct tggccatgta acctctgatc tatagaattt tttaaatgac tagaattaat
     1081 gcccatcttt tttttggacc taaattcttc atgaaaatat attacgaggg cttattcaga
     1141 agctttggac ttcttcgcca gaggtttggt caagtctcca atcaaggttg tcggcttgtc
     1201 taccttgcca gaaatttacg aaaagatgga aaagggtcaa atcgttggta gatacgttgt
     1261 tgacacttct aaataagcga atttcttatg atttatgatt tttattatta aataagttat
     1321 aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt
     1381 gttcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc
     1441 tcttattgac cacacctcta ccggcatgcc cgaaattccc ctaccctatg aacatattcc
     1501 attttgtaat ttcgtgtcgt ttctattatg aatttcattt ataaagttta tgtacaaata
     1561 tcataaaaaa agagaatctt tttaagcaag gattttctta acttcttcgg cgacagcatc
     1621 accgacttcg gtggtactgt tggaaccacc taaatcacca gttctgatac ctgcatccaa
     1681 aaccttttta actgcatctt caatggcctt accttcttca ggcaagttca atgacaattt
     1741 caacatcatt gcagcagaca agatagtggc gatagggtca accttattct ttggcaaatc
     1801 tggagcagaa ccgtggcatg gttcgtacaa accaaatgcg gtgttcttgt ctggcaaaga
     1861 ggccaaggac gcagatggca acaaacccaa ggaacctggg ataacggagg cttcatcgga
     1921 gatgatatca ccaaacatgt tgctggtgat tataatacca tttaggtggg ttgggttctt
     1981 aactaggatc atggcggcag aatcaatcaa ttgatgttga accttcaatg taggaaattc
     2041 gttcttgatg gtttcctcca cagtttttct ccataatctt gaagaggcca aaacattagc
     2101 tttatccaag gaccaaatag gcaatggtgg ctcatgttgt agggccatga aagcggccat
     2161 tcttgtgatt ctttgcactt ctggaacggt gtattgttca ctatcccaag cgacaccatc
     2221 accatcgtct tcctttctct taccaaagta aatacctccc actaattctc tgacaacaac
     2281 gaagtcagta cctttagcaa attgtggctt gattggagat aagtctaaaa gagagtcgga
     2341 tgcaaagtta catggtctta agttggcgta caattgaagt tctttacgga tttttagtaa
     2401 accttgttca ggtctaacac tacctgtacc ccatttagga ccacccacag cacctaacaa
     2461 aacggcatca accttcttgg aggcttccag cgcctcatct ggaagtggga cacctgtagc
     2521 atcgatagca gcaccaccaa ttaaatgatt ttcgaaatcg aacttgacat tggaacgaac
     2581 atcagaaata gctttaagaa ccttaatggc ttcggctgtg atttcttgac caacgtggtc
     2641 acctggcaaa acgacgatct tcttaggggc agacattaga atggtatatc cttgaaatat
     2701 atatatatat tgctgaaatg taaaaggtaa gaaaagttag aaagtaagac gattgctaac
     2761 cacctattgg aaaaaacaat aggtccttaa ataatattgt caacttcaag tattgtgatg
     2821 caagcattta gtcatgaacg cttctctatt ctatatgaaa agccggttcc ggcctctcac
     2881 ctttcctttt tctcccaatt tttcagttga aaaaggtata tgcgtcaggc gacctctgaa
     2941 attaacaaaa aatttccagt catcgaattt gattctgtgc gatagcgccc ctgtgtgttc
     3001 tcgttatgtt gaggaaaaaa ataatggttg ctaagagatt cgaactcttg catcttacga
     3061 tacctgagta ttcccacagt tggggatctc gactctagct agaggatcaa ttcgtaatca
     3121 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     3181 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt
     3241 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gtaatggccg
     3301 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     3361 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg
     3421 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     3481 acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
     3541 gggactttcc acaccctaac tgacacacat tccacagctg gttctttccg cctcaggact
     3601 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3661 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3721 gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3781 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tccctccctt
     3841 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     3901 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     3961 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4021 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4081 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4141 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttacgcgc ctaaattatg
     4201 cggccgcata taactggatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     4261 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     4321 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     4381 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     4441 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4501 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4561 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4621 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4681 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4741 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4801 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4861 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     4921 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     4981 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5041 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5101 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5161 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5221 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5281 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5341 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5401 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5461 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5521 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5581 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5641 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5701 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5761 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     5821 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     5881 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     5941 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6001 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6061 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6121 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6181 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     6241 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     6301 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     6361 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     6421 ggcatcagag cagattgtac tgagagtgca ccataacgca tttaagcata aacacgcact
     6481 atgccgttct tctcatgtat atatatatac aggcaacacg cagatatagg tgcgacgtga
     6541 acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt tttcggaaac
     6601 gctttgaagt tcctattccg aagttcctat tctctagcta gaaagtatag gaacttcaga
     6661 gcgcttttga aaaccaaaag cgctctgaag acgcactttc aaaaaaccaa aaacgcaccg
     6721 gactgtaacg agctactaaa atattgcgaa taccgcttcc acaaacattg ctcaaaagta
     6781 tctctttgct atatatctct gtgctatatc cctatataac ctacccatcc acctttcgct
     6841 ccttgaactt gcatctaaac tcgacctcta cattttttat gtttatctct agtattactc
     6901 tttagacaaa aaaattgtag taagaactat tcatagagtg aatcgaaaac aatacgaaaa
     6961 tgtaaacatt tcctatacgt agtatataga gacaaaatag aagaaaccgt tcataatttt
     7021 ctgaccaatg aagaatcatc aacgctatca ctttctgttc acaaagtatg cgcaatccac
     7081 atcggtatag aatataatcg gggatgcctt tatcttgaaa aaatgcaccc gcagcttcgc
     7141 tagtaatcag taaacgcggg aagtggagtc aggctttttt tatggaagag aaaatagaca
     7201 ccaaagtagc cttcttctaa ccttaacgga cctacagtgc aaaaagttat caagagactg
     7261 cattatagag cgcacaaagg agaaaaaaag taatctaaga tgctttgtta gaaaaatagc
     7321 gctctcggga tgcatttttg tagaacaaaa aagaagtata gattctttgt tggtaaaata
     7381 gcgctctcgc gttgcatttc tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa
     7441 ttagcgctct cgcgttgcat ttttgtttta caaaaatgaa gcacagattc ttcgttggta
     7501 aaatagcgct ttcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg
     7561 aaaaattagc gctctcgcgt tgcatttttg ttctacaaaa tgaagcacag atgcttcgtt
//
