LOCUS       V009583                 3854 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009583
VERSION     V009583
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Anderson JC, Voigt CA, Arkin AP.
  TITLE     Environmental signal integration by a modular AND gate
  JOURNAL   Mol. Syst. Biol. 3, 133 (2007)
   PUBMED   17700541
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Salis HM, Mirsky EA, Voigt CA.
  TITLE     Automated design of synthetic ribosome binding sites to control
            protein expression
  JOURNAL   Nat. Biotechnol. 27 (10), 946-950 (2009)
   PUBMED   19801975
REFERENCE   3  (bases 1 to 3854)
  AUTHORS   Tamsir A, Tabor JJ, Voigt CA.
  TITLE     Robust multicellular computing using genetically encoded NOR gates
            and chemical 'wires'
  JOURNAL   Nature 469 (7329), 212-215 (2011)
   PUBMED   21150903
REFERENCE   4  (bases 1 to 3854)
  AUTHORS   Moser F, Voigt CA.
  TITLE     Genetic Circuit Performance under Conditions Relevant for Industrial
            Bioreactors
  JOURNAL   Unpublished
REFERENCE   5  (bases 1 to 3854)
  AUTHORS   Anderson JC, Moser F, Tamsir A, Salis H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JAN-2012) Biological Engineering, MIT, 500 Tech
            Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE   6  (bases 1 to 3854)
  TITLE     Direct Submission
REFERENCE   7  (bases 1 to 3854)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Syst.
            Biol. 3, 133 (2007)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Nat.
            Biotechnol."; date: "2009"; volume: "27"; issue: "10"; pages:
            "946-950"
            SGRef: number: 3; type: "Journal Article"; journalName: "Nature";
            date: "2011"; volume: "469"; issue: "7329"; pages: "212-215"
            SGRef: number: 4; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 5; type: "Journal Article"; journalName: "Submitted
            (06-JAN-2012) Biological Engineering, MIT, 500 Tech Square, Rm 209D,
            Cambridge, MA 02139, USA"
            SGRef: number: 6; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(221..1120)
                     /gene="nahR"
                     /label="HTH-type transcriptional activator NahR"
                     /note="HTH-type transcriptional activator NahR from
                     Pseudomonas putida. Accession#: P10183"
     gene            1282..1371
                     /gene="supD"
                     /label="supD"
     CDS             1431..1460
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             1476..1493
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      1719..1765
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1946..2602)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2603..2705)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(3231..3776)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
ORIGIN
        1 ggatcgcggc cgctgcgatc ccgcgaagaa ccaaaaaagc tcgacagagg gcgcggtcat
       61 tttaggtcgg gcggatcggc gccgccggct cggctggtgt gccgcacagc accgcctacg
      121 tgagctgcca gttgatgaac ttcccccgtt gccagctagg gcgcaagcgg gctgtataag
      181 atcactgccc atcacattga tcggctcgga ttttttctca atccgtaaac aggtcaaaca
      241 tcagttgccg caaccaaata ttggctaggt ccttgtggta cttcgcatgc cagaacatgt
      301 tgatggctat ttcaggcaag acgactgggt gcggcaaggc gcttaggccg aagggctcca
      361 cgcagcagtc ggctaaacgt atcggcacag tggcgagcag atcggtgcgc tggaggatgt
      421 ggccaacggc ggcgaagtgc ggcacttcca gacggatgtc gcgccggatg ccgacccgtg
      481 tcatgtacgt gtccacctcg ccgtggccgg tgccagcggc gatgacacgc acgtggccgt
      541 aggaacagaa gcgctccaga gtcaggggtt cgcgggtgac tggatggtcc ttgcgacata
      601 ggcacacgta gtgattctgg agcagccggc gctgaaagaa gccagtttgc agattgggaa
      661 gcaggcccac ggccaagtcc acggttccgt tctgcaaggc ctgcatcagg ctcatcgaac
      721 tgtcgcgcac cgtactgatc acgcaattgg gggcctggtg agccagcaca tccatcagcc
      781 gcggcatgaa gtagatctcg ccaatgtcgg tcatggccag ggtgaaggta cgctcgctgg
      841 tcagcggatc gaagctttca tggtgctgta gggcgttgcg cagtgcgtgc atggccgaag
      901 tgacgggctc ggccagatgc gcggcatagg gtgtgggttc cattccctga tgtgtgcgca
      961 cgaagagtgg gtcctgtagc gaggtgcgca ggcgtttcag cgcattgctc acggcaggct
     1021 gggtcaggcc caggttctcc gcagtgatag agacgcgtct gtcgaccagc aactggttga
     1081 acaccaccag caggtttaaa tccaggtcac gcagttccat ggggcctcgc ttgggttatt
     1141 gctggtgccc ggccgggcgc aatattcatg ttgatgattt attatatatc gagtggtgta
     1201 tttatcaata ttgtttgctc cgttatcgtt attaacaagt catcaataaa gccatcacga
     1261 gtaccataga ggatccaatt cggagagatg ccggagcggc tgaacggacc ggtctctaaa
     1321 accggagtag gggcaactct accgggggtt caaatccccc tctctccgcc actgcagatc
     1381 cttagcgaaa gctaaggatt ttttttaagc tgaattcgaa gcttgggccc gaacaaaaac
     1441 tcatctcaga agaggatctg aatagcgccg tcgaccatca tcatcatcat cattgagttt
     1501 aaacggtctc cagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat
     1561 taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt
     1621 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     1681 ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
     1741 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaactaattc gaagcttgac
     1801 ataagcggct atttaacgac cctgccctga accgacgacc gggtcgaatt tgctttcgaa
     1861 tttctgccat tcatccgctt attatcactt attcaggcgt agcaccaggc gtttaagggc
     1921 accaataact gccttaaaaa aattacgccc cgccctgcca ctcatcgcag tactgttgta
     1981 attcattaag cattctgccg acatggaagc catcacagac ggcatgatga acctgaatcg
     2041 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatcgtg aaaacggggg
     2101 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     2161 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     2221 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     2281 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     2341 cactatccca tatcaccagc tcaccgtctt tcattgccat acggaactcc ggatgagcat
     2401 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     2461 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     2521 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     2581 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     2641 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     2701 gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac
     2761 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg
     2821 cgtcgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca
     2881 gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac
     2941 ggcaaaagca ccgccggaca tcagcgctag cggagtgtat actggcttac tatgttggca
     3001 ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg
     3061 tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc
     3121 ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga
     3181 tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg
     3241 ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg
     3301 acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt
     3361 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
     3421 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     3481 cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag
     3541 acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga
     3601 agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa
     3661 gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca
     3721 aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag
     3781 atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc tcttcaaatg
     3841 tagcacctga agtc
//
