LOCUS       40924_4001        8937 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pACYC_synB_polyST_cstII_lgtB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8937)
  AUTHORS   Keys TG, Wetter M, Hang I, Rutschmann C, Russo S, Mally M, Steffen 
            M, Zuppiger M, Muller F, Schneider J, Faridmoayer A, Lin CW, Aebi M.
  TITLE     A biosynthetic route for polysialylating proteins in Escherichia 
            coli
  JOURNAL   Metab. Eng. (2017) In press
  PUBMED    29101090
REFERENCE   2  (bases 1 to 8937)
  AUTHORS   Keys TG.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-NOV-2016) Institute of Microbiology, ETH Zurich, 
            Vladimir-Prelog-Weg 4, Zurich, ZH 8093, Switzerland
REFERENCE   3  (bases 1 to 8937)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8937)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-NOV-2016) Institute of Microbiology, ETH Zurich, 
            Vladimir-Prelog-Weg 4, Zurich, ZH 8093, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      28..75
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(298..954)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(955..1057)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(1583..2127)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     protein_bind    complement(2318..2339)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2355..3434)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(3435..3512)
                     /label=lacI promoter
     promoter        3550..3580
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    3584..3608
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3656..4342
                     /codon_start=1
                     /product="SynB"
                     /label=SynB
                     /protein_id="ATN32188.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKCF
                     DRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTLLQ
                     PTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSDLEQ
                     PRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQAENI
                     LNHKES"
     promoter        4357..4387
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     regulatory      4357..4362
                     /regulatory_class="minus_35_signal"
     regulatory      4381..4387
                     /note="-10 seq (TATAAT; Pribnow box)"
                     /regulatory_class="minus_10_signal"
     protein_bind    4391..4415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    4393..4413
                     /label=lac operator
                     /note="lac operator"
     CDS             4463..5884
                     /codon_start=1
                     /product="polyST F116-F460T"
                     /label=polyST F116-F460T
                     /protein_id="ATN32189.1"
                     /translation="MGSPSYLSPPRNNLFVISNLGQLNQVQSLIKIQKLTDNLLVILYT
                     SQNLKMPKLVHQSANKNLFESIYLFELPRSPNIITPKKLLYIYHSYKKILNIIQPAHLY
                     MLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAPLLESFSYHPTKLERYLIGNNLNIKG
                     YIDHFDILHVPFPEYAKKIFNAKKYNRFFAHAGGISVNNNIANLQKKYQISKNDYIFVS
                     QRYPISDDLYYKSIVEILNSISLQIKGKIFIKLHPKEMGNNYVMSLFLNMVEINPRLVV
                     INEPPFLIEPLIYLTNPKGIIGLASSSLIYTPLLSPSTQCLSIGELIINLIQKYSMVEN
                     TEMIQVHLEIIKKFNFVNILNDLNGVNSTPLYKTEETFETLLKSAEFAYISKNYFQAIF
                     YWQLASKNNITLLGHKALWYYNALYNVKQIYKMEYSDITYIDNISVDFHSKDKLTWEKI
                     KHYYYSADNRIGRDR"
     promoter        5896..5926
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     regulatory      5896..5901
                     /regulatory_class="minus_35_signal"
     regulatory      5920..5926
                     /note="-10 seq (TATAAT; Pribnow box)"
                     /regulatory_class="minus_10_signal"
     protein_bind    5930..5954
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    5932..5952
                     /label=lac operator
                     /note="lac operator"
     CDS             6002..6781
                     /codon_start=1
                     /product="CstII"
                     /label=CstII
                     /note="delta-C32 and I53S mutation"
                     /protein_id="ATN32190.1"
                     /translation="MKKVIIAGNGPSLKEIDYSRLPNDFDVFRCNQFYFEDKYYLGKKC
                     KAVFYNPSLFFEQYYTLKHLIQNQEYETELIMCSNYNQAHLENENFVKTFYDYFPDAHL
                     GYDFFKQLKDFNAYFKFHEIYFNQRITSGVYMCAVAIALGYKEIYLSGIDFYQNGSSYA
                     FDTKQKNLLKLAPNFKNDNSHYIGHSKNTDIKALEFLEKTYKIKLYCLCPNSLLANFIG
                     LAPNLNSNFIIQEKNNYTKDILIPSSEAYGKFSKNIN"
     promoter        6793..6823
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     regulatory      6793..6798
                     /regulatory_class="minus_35_signal"
     regulatory      6817..6823
                     /note="-10 seq (TATAAT; Pribnow box)"
                     /regulatory_class="minus_10_signal"
     protein_bind    6827..6851
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    6829..6849
                     /label=lac operator
                     /note="lac operator"
     CDS             6899..7729
                     /codon_start=1
                     /product="LgtB"
                     /label=LgtB
                     /protein_id="ATN32191.1"
                     /translation="MSQNHVISLASAAERRAHIADTFGRHGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYITVFEDDVLLGEGAEKFLAE
                     DAWLQERFDPDTAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISRKA
                     MRFFLDRFAALPPEGLHPVDLMMFSDFFDREGMPVCQLNPALCAQELHYAKFHDQNSAL
                     GSLIEHDRLLNRKQQRRDSPANTFKHRLIRALTKISREREKRRQRREQFIVPFQ"
ORIGIN
        1 taggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
       61 ttgaggggtt ttttgctgaa acctcaggca tttgagaagc acacggtcac actgcttccg
      121 gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct
      181 gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc ttattatcac
      241 ttattcaggc gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc
      301 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
      361 gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
      421 cgtataatat ttgcccatag tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
      481 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
      541 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
      601 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
      661 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
      721 tttcattgcc atacggaact ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
      781 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
      841 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
      901 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
      961 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     1021 attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt
     1081 ggcccagggc ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc
     1141 ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca acttactgat
     1201 ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct
     1261 cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct
     1321 agcggagtgt atactggctt actatgttgg cactgatgag ggtgtcagtg aagtgcttca
     1381 tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat
     1441 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg
     1501 gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg
     1561 agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa
     1621 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1681 ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc
     1741 gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc
     1801 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg
     1861 gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca
     1921 ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa
     1981 aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta
     2041 gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag
     2101 attacgcgca gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttc
     2161 tagatttcag tgcaatttat ctcttcaaat gtagcacctg aagtcagccc catacgatat
     2221 aagttgtaat tctcatgtta gtcatgcccc gcgcccaccg gaaggagctg actgggttga
     2281 aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa
     2341 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     2401 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt
     2461 ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc
     2521 agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac
     2581 ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatgtccgca
     2641 ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg
     2701 gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa
     2761 ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg
     2821 agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct
     2881 aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg
     2941 tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac
     3001 gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag
     3061 ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct
     3121 tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga
     3181 gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg
     3241 ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc
     3301 agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg
     3361 ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac
     3421 gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata
     3481 ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct cgacgctctc ccttacataa
     3541 acgctagcct ttacacttta tgcttccggc tcgtataatg tgtggaattg tgagcggata
     3601 acaattcccc actagaaata agcaaaagaa aaagacaagg aggtacctgc taagcatgga
     3661 gaaacaaaac attgctgtta tcctggcgcg tcaaaattcc aaaggtctgc ctttgaagaa
     3721 tctgcgtaag atgaacggca tctccttgtt gggtcacact atcaatgctg caatctccag
     3781 caaatgcttc gatcgtatca tcgtttcgac cgacggtggt ctgattgcgg aagaggcgaa
     3841 aaattttggc gtggaagttg tcctgcgtcc ggccgaatta gcgtctgaca cggcgagctc
     3901 catctctggt gtgatccacg cgctggaaac gattggctcc aactccggta ccgtcacctt
     3961 gctgcaaccg accagcccac tgcgtacggg cgctcatatt cgtgaggcgt ttagcctgtt
     4021 cgatgagaag attaagggca gcgtggtgtc cgcgtgcccg atggaacacc acccgttaaa
     4081 aaccctcctg cagatcaata atggtgaata tgcgccgatg cgccacttgt ccgacttgga
     4141 gcaaccgcgc cagcaactgc cgcaagcttt ccgtccaaat ggcgcaatct atatcaacga
     4201 caccgcaagc ctgatcgcca ataactgctt tttcattgct ccaaccaaac tgtacattat
     4261 gagccaccag gactcgattg atattgatac cgaactggat ctgcagcagg cggaaaacat
     4321 tctcaaccat aaggagagct aagaagacca ctaggcttta cactttatgc ttccggctcg
     4381 tataatgtgt ggaattgtga gcggataaca attccccact agaaataagg gtactatatc
     4441 cataactaag aaggtcaatc ccatgggttc cccgtcctac ctctccccgc ctcgtaataa
     4501 cttgtttgtg atctccaatt tgggtcaact gaaccaggtg caatctttga tcaagatcca
     4561 gaaactgacc gataacctgt tagtgatcct gtacaccagc caaaatctga aaatgccgaa
     4621 gttggttcat caatccgcga acaagaatct gtttgagagc atctacctgt tcgaattgcc
     4681 gcgttccccg aacattatta ccccgaagaa actgttgtac atctatcact cctacaagaa
     4741 aatcctgaat atcattcagc cggcacacct gtatatgttg tcctttaccg gtcactattc
     4801 ctacctgatt tcaatcgcga agaagaaaaa catcactacc cacctgatcg acgagggtac
     4861 cggtacgtat gctccgttgc tggagagctt ctcttatcac ccgacgaagc tggaacgtta
     4921 cctgatcggc aataacctga acatcaaagg ctacattgac cattttgata ttctgcacgt
     4981 tccgttccca gagtatgcta aaaagatttt caacgcgaag aaatataacc gcttctttgc
     5041 gcatgctggc ggcatttctg tcaataacaa tattgcgaat ctgcaaaaga aataccagat
     5101 ttccaaaaat gactacatct ttgttagcca gcgttatccg atctccgacg atctgtatta
     5161 caaatccatt gtggagattt tgaactccat ttccttacaa atcaaaggta aaatctttat
     5221 caaattgcat ccgaaggaaa tgggcaataa ctatgtgatg tctttgttcc tcaacatggt
     5281 tgagattaat ccgcgtctgg ttgtgattaa cgaacctccg tttctgatcg agccgttgat
     5341 ctatttgacc aatcctaaag gcattatcgg tctggcgtcc agctccttga tctacactcc
     5401 gctgctctct ccgtccacgc aatgcctgag cattggtgag ctgattatca acttgattca
     5461 aaaatactct atggttgaaa acaccgagat gattcaggtc catctggaga ttatcaagaa
     5521 gtttaatttc gtcaacatct taaacgactt gaacggtgtt aactccaccc cgttgtacaa
     5581 gaccgaggaa accttcgaaa ccctcttgaa atccgcggaa tttgcttaca taagcaagaa
     5641 ctatttccag gcgatctttt actggcaatt ggcgtccaag aataatatca ccctgttagg
     5701 tcataaggcg ttgtggtact acaatgccct gtataacgtt aaacaaatct acaaaatgga
     5761 gtattccgac atcacataca ttgataatat ctccgttgac ttccatagca aagataagct
     5821 gacctgggaa aagatcaagc actattacta tagcgcagac aaccgtatcg gccgtgatcg
     5881 ctaagcactc tagactttac actttatgct tccggctcgt ataatgtgtg gaattgtgag
     5941 cggataacaa ttccccacta gaaataattc aacaacaaac ttactcagag gagctaacat
     6001 catgaagaag gttattatcg ctggcaatgg cccgtcgctg aaggagattg actattcccg
     6061 tttgcctaat gatttcgacg tgtttcgctg caaccaattt tatttcgagg acaaatatta
     6121 cttgggtaag aaatgtaagg ctgtttttta caacccgagc ctgttctttg aacaatacta
     6181 tacgctgaag cacctgatcc agaatcaaga atacgaaacg gagttgatca tgtgcagcaa
     6241 ttacaaccag gcccacttgg agaatgaaaa ctttgtgaaa accttttatg actactttcc
     6301 ggatgcgcat ctgggttacg atttctttaa gcagctcaaa gatttcaacg cttattttaa
     6361 gttccacgag atttacttca accagcgcat cacctctggc gtgtatatgt gcgcggtcgc
     6421 gatcgcgctg ggctacaaag aaatctacct ctccggtatc gacttctacc agaacggtag
     6481 ctcgtacgcg ttcgacacca aacagaagaa tctgttgaag ctggcaccga acttcaaaaa
     6541 cgacaattct cactatattg gtcatagcaa gaacaccgac atcaaagcgc tggaattttt
     6601 ggaaaaaacc tataagatta aactgtactg cctgtgtcca aatagcttgt tagcaaactt
     6661 cattggtttg gcgccgaatc tgaatagcaa cttcattatt caagagaaga ataactacac
     6721 caaggacatc ttaattccgt ccagcgaagc gtatggcaag ttctccaaga acatcaacta
     6781 agcacactag tctttacact ttatgcttcc ggctcgtata atgtgtggaa ttgtgagcgg
     6841 ataacaattc cccactagaa ataactcttt aatactattt acataaggag gcgagaacat
     6901 gtctcaaaac catgtgatct ctttggcatc tgcggccgag cgtcgcgcgc acatcgcgga
     6961 taccttcggc cgtcatggca ttccatttca attttttgac gcgctcatgc catccgaacg
     7021 tttagagcaa gcgatggcgg aactggtgcc aggtttgtcc gcgcatccgt acctcagcgg
     7081 cgttgagaaa gcttgtttta tgtcgcacgc cgttctgtgg aagcaggcgt tagacgaggg
     7141 tctgccgtat attaccgttt tcgaggacga cgttttgttg ggtgaaggtg cggaaaagtt
     7201 tttggctgag gacgcttggc tgcaggagcg ttttgatcct gatacggcct tcattgttcg
     7261 cctggaaacc atgttcatgc atgtcttgac ctccccgagc ggtgtcgccg actactgtgg
     7321 tcgcgctttc ccgttgctgg agagcgagca ctggggcacc gcgggttata ttatctcccg
     7381 taaagcaatg cgtttcttcc tggatcgctt cgctgcgctg ccgccggagg gcttgcaccc
     7441 ggtggacctg atgatgttca gcgatttttt cgaccgcgag ggtatgcctg tttgccaact
     7501 gaatccggcg ttgtgcgcgc aggagttaca ctacgcaaaa ttccacgatc agaacagcgc
     7561 gctgggtagc ctgatcgagc atgaccgtct gctgaaccgc aaacagcaac gccgtgacag
     7621 cccggctaac acctttaagc accgtctgat ccgcgcgctg actaagatca gccgtgaacg
     7681 tgagaagcgt cgtcagcgtc gcgaacaatt cattgtgccg tttcaataat tcgcctaccg
     7741 tgaacaagca tgatgtcaaa cgtgcgctga accatgtgat tcgccgtcat atcgagtccg
     7801 aatacggctg gaaagaccgt tacgtggcgc acatcggcta ccgtaacaac aagccggtga
     7861 tggttgttct gctggagcac tttcactctg cacactccat ctatcgtacc cactcgacct
     7921 ccatgattgc ggcccgcgag cacttttacc tgatcggttt gggtagcccg agcgttgatc
     7981 aagcgggcca agaggttttt gacgagtttc atctcgttgc gggtgataat atgaagcaga
     8041 agctggagtt cattcgttcg gtgtgcgagt ctaacggtgc cgccatcttc tacatgccgt
     8101 ccattggcat ggatatgacc accatcttcg cttcgaatac tcgccttgcg cctatccagg
     8161 cgatcgcact gggccaccct gccaccaccc acagcgactt tatcgagtac gtcatcgttg
     8221 aggacgatta cgtgggctcc gaggcatgct ttagcgaaac gttgctgaga ctgccgaagg
     8281 acgcgctgcc gtatgtcccg tccgccttgg ccccggagaa agttgattat cttttgcgtg
     8341 agaatccaga ggtcgttaat attggcattg cgagcaccac catgaagctg aacccttatt
     8401 tcttggaagc gctgaaggcg atccgtgatc gtgctaaagt caaagttcac ttccattttg
     8461 cgctgggcca aagcaacggt attacccatc catacgttga gcgtttcatt aagtcctacc
     8521 tgggtgatag cgcgaccgct cacccacatt ccccgtatca ccaatacttg cgcatcttgc
     8581 ataattgcga catgatggtt aacccgttcc cgtttggtaa cacgaatggc atcattgata
     8641 tggtgaccct cggtctggtt ggtgtttgca aaaccggtgc agaggtccac gaacacatcg
     8701 acgagggctt gtttaagcgt ctgggcttgc cggaatggct gattgccaac accgttgacg
     8761 aatatgtgga gcgtgcggtg cgtttggcgg aaaaccacca ggaacgcttg gagctgcgcc
     8821 gctatattat cgagaataat ggcttgaata cgctgtttac cggtgacccg cgtccgatgg
     8881 gtcaggtttt tctggaaaaa ttgaacgctt tcttgaagga gaactaatag cgtctct
//
