LOCUS       V009587                 5528 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009587
VERSION     V009587
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Keys TG, Wetter M, Hang I, Rutschmann C, Russo S, Mally M, Steffen
            M, Zuppiger M, Muller F, Schneider J, Faridmoayer A, Lin CW, Aebi M.
  TITLE     A biosynthetic route for polysialylating proteins in Escherichia
            coli
  JOURNAL   Metab. Eng. (2017) In press
   PUBMED   29101090
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   Keys TG.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-NOV-2016) Institute of Microbiology, ETH Zurich,
            Vladimir-Prelog-Weg 4, Zurich, ZH 8093, Switzerland
REFERENCE   3  (bases 1 to 5528)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5528)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            (2017) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-NOV-2016) Institute of Microbiology, ETH Zurich,
            Vladimir-Prelog-Weg 4, Zurich, ZH 8093, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      28..75
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     CDS             complement(298..954)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(955..1057)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(1583..2127)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     protein_bind    complement(2318..2339)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2355..3434)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(3435..3512)
                     /label="lacI promoter"
     promoter        3550..3580
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    3584..3608
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3656..5515
                     /note="UDP-glucose:protein N-beta-glucosyltransferase from
                     Actinobacillus pleuropneumoniae serotype 7 (strain AP76).
                     Accession#: B3H2N2"
                     /label="UDP-glucose:protein N-beta-glucosyltransferase"
ORIGIN
        1 taggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
       61 ttgaggggtt ttttgctgaa acctcaggca tttgagaagc acacggtcac actgcttccg
      121 gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct
      181 gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc ttattatcac
      241 ttattcaggc gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc
      301 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
      361 gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
      421 cgtataatat ttgcccatag tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
      481 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
      541 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
      601 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
      661 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
      721 tttcattgcc atacggaact ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
      781 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
      841 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
      901 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
      961 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     1021 attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt
     1081 ggcccagggc ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc
     1141 ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca acttactgat
     1201 ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct
     1261 cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct
     1321 agcggagtgt atactggctt actatgttgg cactgatgag ggtgtcagtg aagtgcttca
     1381 tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat
     1441 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg
     1501 gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg
     1561 agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa
     1621 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1681 ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc
     1741 gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc
     1801 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg
     1861 gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca
     1921 ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa
     1981 aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta
     2041 gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag
     2101 attacgcgca gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttc
     2161 tagatttcag tgcaatttat ctcttcaaat gtagcacctg aagtcagccc catacgatat
     2221 aagttgtaat tctcatgtta gtcatgcccc gcgcccaccg gaaggagctg actgggttga
     2281 aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa
     2341 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     2401 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt
     2461 ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc
     2521 agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac
     2581 ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatgtccgca
     2641 ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg
     2701 gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa
     2761 ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg
     2821 agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct
     2881 aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg
     2941 tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac
     3001 gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag
     3061 ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct
     3121 tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga
     3181 gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg
     3241 ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc
     3301 agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg
     3361 ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac
     3421 gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata
     3481 ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct cgacgctctc ccttacataa
     3541 acgctaggct ttacacttta tgcttccggc tcgtataatg tgtggaattg tgagcggata
     3601 acaattcccc actagaaata aacccccaga cccacagata ctcaggaggt ctcgcatgga
     3661 gaacgagaat aagcctaatg ttgcaaactt tgaggcggca gttgctgtca aagactacga
     3721 gaaggcttgc tccgagctgt tgctgatctt gtctcagctg gatagcaact ttggcggtat
     3781 tcaagaaatc gagttcgagt acccggttca actccaggac ctcgaacaag agaaaattgt
     3841 atacttctgc acccgcatgg cgaccgcgat cacgaccttg ttttccgacc ctgttctgga
     3901 gatcagcgac ctgggtgttc agcgtttcct ggtttaccaa cgttggctcg cattgatctt
     3961 tgcatcgtcc ccgtttgtta acgcggatca tattctgcaa acctataacc gcgaaccgaa
     4021 ccgtaaaaac tctttggaaa tccatctgga ctctagcaaa tccagcctca tcaaattttg
     4081 cattttatac ttgccggagt ccaacgtcaa cctgaacttg gacgtgatgt ggaacattag
     4141 cccggagttg tgcgccagcc tgtgcttcgc actgcagtcg ccgcgcttca tcggcacctc
     4201 taccgcgttc aacaagcgtg cgactatttt acaatggttt ccacgtcacc tggaccagct
     4261 gaaaaatttg aacaacattc cgagcgcgat ttcccacgac gtctatatgc attgctctta
     4321 cgacacctcc gtgaacaagc atgatgtcaa acgtgcgctg aaccatgtga ttcgccgtca
     4381 tatcgagtcc gaatacggct ggaaagaccg ttacgtggcg cacatcggct accgtaacaa
     4441 caagccggtg atggttgttc tgctggagca ctttcactct gcacactcca tctatcgtac
     4501 ccactcgacc tccatgattg cggcccgcga gcacttttac ctgatcggtt tgggtagccc
     4561 gagcgttgat caagcgggcc aagaggtttt tgacgagttt catctcgttg cgggtgataa
     4621 tatgaagcag aagctggagt tcattcgttc ggtgtgcgag tctaacggtg ccgccatctt
     4681 ctacatgccg tccattggca tggatatgac caccatcttc gcttcgaata ctcgccttgc
     4741 gcctatccag gcgatcgcac tgggccaccc tgccaccacc cacagcgact ttatcgagta
     4801 cgtcatcgtt gaggacgatt acgtgggctc cgaggcatgc tttagcgaaa cgttgctgag
     4861 actgccgaag gacgcgctgc cgtatgtccc gtccgccttg gccccggaga aagttgatta
     4921 tcttttgcgt gagaatccag aggtcgttaa tattggcatt gcgagcacca ccatgaagct
     4981 gaacccttat ttcttggaag cgctgaaggc gatccgtgat cgtgctaaag tcaaagttca
     5041 cttccatttt gcgctgggcc aaagcaacgg tattacccat ccatacgttg agcgtttcat
     5101 taagtcctac ctgggtgata gcgcgaccgc tcacccacat tccccgtatc accaatactt
     5161 gcgcatcttg cataattgcg acatgatggt taacccgttc ccgtttggta acacgaatgg
     5221 catcattgat atggtgaccc tcggtctggt tggtgtttgc aaaaccggtg cagaggtcca
     5281 cgaacacatc gacgagggct tgtttaagcg tctgggcttg ccggaatggc tgattgccaa
     5341 caccgttgac gaatatgtgg agcgtgcggt gcgtttggcg gaaaaccacc aggaacgctt
     5401 ggagctgcgc cgctatatta tcgagaataa tggcttgaat acgctgttta ccggtgaccc
     5461 gcgtccgatg ggtcaggttt ttctggaaaa attgaacgct ttcttgaagg agaactaata
     5521 gcgtctct
//
