LOCUS       Exported                4010 bp DNA     circular SYN 03-SEP-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pACYCDuet-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        29..47
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    48..72
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             128..145
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        259..277
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    278..302
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             411..455
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      507..554
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(775..1434)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1435..1537)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      complement(2063..2608)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     CDS             complement(2833..3915)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(3916..3993)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 cccttatgcg actcctgcat taggaaatta atacgactca ctatagggga attgtgagcg
       61 gataacaatt cccctgtaga aataattttg tttaacttta ataaggagat ataccatggg
      121 cagcagccat caccatcatc accacagcca ggatccgaat tcgagctcgg cgcgcctgca
      181 ggtcgacaag cttgcggccg cataatgctt aagtcgaaca gaaagtaatc gtattgtaca
      241 cggccgcata atcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt
      301 ccccatctta gtatattagt taagtataag aaggagatat acatatggca gatctcaatt
      361 ggatatcggc cggccacgcg atcgctgacg tcggtaccct cgagtctggt aaagaaaccg
      421 ctgctgcgaa atttgaacgc cagcacatgg actcgtctac tagcgcagct taattaacct
      481 aggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
      541 tgaggggttt tttgctgaaa cctcaggcat ttgagaagca cacggtcaca ctgcttccgg
      601 tagtcaataa accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg
      661 aaccgacgac cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact
      721 tattcaggcg tagcaaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc
      781 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
      841 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
      901 cgtataatat ttgcccatag tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
      961 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     1021 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     1081 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     1141 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     1201 tttcattgcc atacggaact ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     1261 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     1321 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     1381 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     1441 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     1501 attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt
     1561 ggcccagggc ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc
     1621 ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca acttactgat
     1681 ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct
     1741 cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct
     1801 agcggagtgt atactggctt actatgttgg cactgatgag ggtgtcagtg aagtgcttca
     1861 tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat
     1921 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg
     1981 gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg
     2041 agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa
     2101 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2161 cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
     2221 cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt
     2281 cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc
     2341 ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc
     2401 actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga
     2461 aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt
     2521 agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga
     2581 gattacgcgc agaccaaaac gatctcaaga agatcatctt attaatcaga taaaatattt
     2641 ctagatttca gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc ccatacgata
     2701 taagttgtaa ttctcatgtt agtcatgccc cgcgcccacc ggaaggagct gactgggttg
     2761 aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta
     2821 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     2881 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct
     2941 tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg
     3001 cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa
     3061 cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatgtccgc
     3121 accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt
     3181 ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa
     3241 accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt
     3301 gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc
     3361 taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc
     3421 gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa
     3481 cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata
     3541 gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc
     3601 ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg
     3661 agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac
     3721 gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt
     3781 cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg
     3841 gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa
     3901 cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat
     3961 accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct
//
