LOCUS       V009590                 4012 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009590
VERSION     V009590
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y,
            Luzhetskyy A.
  TITLE     From synthetic promoters to new antibiotics: transcriptional
            activation of silent landomycin A cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y,
            Luzhetskyy A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2014) AMEG, Helmholtz Institute for Pharmaceutical
            Research Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes,
            Saarbruecken, Saarland 66123, Germany
REFERENCE   3  (bases 1 to 4012)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4012)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-DEC-2014) AMEG, Helmholtz Institute for Pharmaceutical Research
            Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes,
            Saarbruecken, Saarland 66123, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      434..474
                     /gene="D6"
                     /regulatory_class="promoter"
     gene            434..474
                     /gene="D6"
                     /label="D6"
     misc_feature    475..524
                     /label="P-GG"
                     /note="P-GG"
     CDS             632..1627
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     misc_feature    1637..1687
                     /label="B-CC"
                     /note="B-CC"
     regulatory      1694..1734
                     /gene="D11"
                     /regulatory_class="promoter"
     gene            1694..1734
                     /gene="D11"
                     /label="D11"
     primer_bind     complement(1791..1807)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1815..1831)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1839..1869)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1884..1905)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2193..2781)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2955..3812)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3813..3917)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ccagctgtgt aggctggagc
      421 tgcttcgtct agattacgta tcctatggag tccataatgt tccaagcctc gttactacgc
      481 ccccaactga gagaactccc aggttacccc agttggggca ctacgatgta aatacttgac
      541 atatcactgt gattcacata taatatgcgc aggtcggctg gttggctgac aaagtgcgta
      601 ggatgactgt cgagcaacgg gaggtacgga catgacacaa gaatccctgc tccttctcga
      661 ccgtattgat tcggatgatt cctacgcgag cctgcggaac gaccaggaat tctgggagcc
      721 gctggcccgc cgagccctgg aggagctcgg gctgccggtg ccgccggtgc tgcgggtgcc
      781 cggcgagagc accaaccccg tactggtcgg cgagcccggc ccggtgatca agctgttcgg
      841 cgagcactgg tgcggtccgg agagcctcgc gtcggagtca gaggcgtacg cggtcctggc
      901 ggacgccccg gtgccggtgc cccgcctcct cggccgcggc gagctgcggc ccggcaccag
      961 agcctggccg tggccctacc tggtgatgag ccggatgacc ggcaccacct ggcggtccgc
     1021 gatggacggc acgaccgacc ggaacgcgct gctcgccctg gcccgcgaac taggccgggt
     1081 gctcggccgg ctgcacaggg tgccgctgac cgggaacacc gtgctcaccc cccattccga
     1141 ggtcttcccg gaactgctgc gggaacgccg cgcggcgacc gtcgaggacc accgcgggtg
     1201 gggctacctc tcgccccggc tgctggaccg cctggaggac tggctgccgg acgtggacac
     1261 gctgctggcc ggccgcgaac cccggttcgt ccacggcgac ctgcacggga ccaacatctt
     1321 cgtggacctg gccgcgaccg aggtcaccgg gatcgtcgac ttcaccgacg tctatgcggg
     1381 agactcccgc tacagcctgg tgcaactgca tctcaacgcc ttccggggcg accgcgagat
     1441 cctggccgcg ctgctcgacg gggcgcagtg gaagcggacc gaggacttcg cccgcgaact
     1501 gctcgccttc accttcctgc acgacttcga ggtgttcgag gagaccccgc tggatctctc
     1561 cggcttcacc gatccggagg aactggcgca gttcctctgg gggccgccgg acaccgcccc
     1621 cggcgcctga tacatcccgc ggtgcgggtg ccagggcgtg cccccgggct ccccgggcgc
     1681 gtactccctg cagacgccgg gctacaaaaa taatatcaga actaggatgg actcctgcag
     1741 tctagagggt cgacggatcc ccggaacagc tgaagcttgg cgtaatcatg gtcatagctg
     1801 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     1861 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     1921 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     1981 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     2041 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2101 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2161 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2221 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2281 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2341 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2401 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2461 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2521 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2581 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     2641 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2701 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2761 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2821 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     2881 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2941 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3001 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3061 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3121 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3181 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3241 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3301 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3361 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3421 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3481 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3541 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     3601 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3661 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3721 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     3781 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     3841 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     3901 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     3961 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
