LOCUS       V009601                 4020 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009601
VERSION     V009601
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y,
            Luzhetskyy A.
  TITLE     From synthetic promoters to new antibiotics: transcriptional
            activation of silent landomycin A cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y,
            Luzhetskyy A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2014) AMEG, Helmholtz Institute for Pharmaceutical
            Research Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes,
            Saarbruecken, Saarland 66123, Germany
REFERENCE   3  (bases 1 to 4020)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4020)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-DEC-2014) AMEG, Helmholtz Institute for Pharmaceutical Research
            Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes,
            Saarbruecken, Saarland 66123, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      445..488
                     /gene="A1-14"
                     /regulatory_class="promoter"
     gene            445..488
                     /gene="A1-14"
                     /label="A1-14"
     misc_feature    495..545
                     /label="B-CC"
                     /note="B-CC"
     CDS             653..1648
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     misc_feature    1658..1707
                     /label="P-GG"
                     /note="P-GG"
     regulatory      1708..1748
                     /gene="P21"
                     /regulatory_class="promoter"
     gene            1708..1748
                     /gene="P21"
                     /label="P21"
     primer_bind     complement(1799..1815)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1823..1839)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1847..1877)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1892..1913)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2201..2789)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2963..3820)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3821..3925)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ccagctgttc cggggatccg
      421 tcgaccctct agggtaccct gcagcgcata tcctaacact ccccaaatac atcgtcaata
      481 cgacccacct gcagggagta cgcgcccggg gagcccgggg gcacgccctg gcacccgcac
      541 cgcgggatgt aaatacttga catatcactg tgattcacat ataatatgcg caggtcggct
      601 ggttggctga caaagtgcgt aggatgactg tcgagcaacg ggaggtacgg acatgacaca
      661 agaatccctg ctccttctcg accgtattga ttcggatgat tcctacgcga gcctgcggaa
      721 cgaccaggaa ttctgggagc cgctggcccg ccgagccctg gaggagctcg ggctgccggt
      781 gccgccggtg ctgcgggtgc ccggcgagag caccaacccc gtactggtcg gcgagcccgg
      841 cccggtgatc aagctgttcg gcgagcactg gtgcggtccg gagagcctcg cgtcggagtc
      901 agaggcgtac gcggtcctgg cggacgcccc ggtgccggtg ccccgcctcc tcggccgcgg
      961 cgagctgcgg cccggcacca gagcctggcc gtggccctac ctggtgatga gccggatgac
     1021 cggcaccacc tggcggtccg cgatggacgg cacgaccgac cggaacgcgc tgctcgccct
     1081 ggcccgcgaa ctaggccggg tgctcggccg gctgcacagg gtgccgctga ccgggaacac
     1141 cgtgctcacc ccccattccg aggtcttccc ggaactgctg cgggaacgcc gcgcggcgac
     1201 cgtcgaggac caccgcgggt ggggctacct ctcgccccgg ctgctggacc gcctggagga
     1261 ctggctgccg gacgtggaca cgctgctggc cggccgcgaa ccccggttcg tccacggcga
     1321 cctgcacggg accaacatct tcgtggacct ggccgcgacc gaggtcaccg ggatcgtcga
     1381 cttcaccgac gtctatgcgg gagactcccg ctacagcctg gtgcaactgc atctcaacgc
     1441 cttccggggc gaccgcgaga tcctggccgc gctgctcgac ggggcgcagt ggaagcggac
     1501 cgaggacttc gcccgcgaac tgctcgcctt caccttcctg cacgacttcg aggtgttcga
     1561 ggagaccccg ctggatctct ccggcttcac cgatccggag gaactggcgc agttcctctg
     1621 ggggccgccg gacaccgccc ccggcgcctg atacatcgta gtgccccaac tggggtaacc
     1681 tgggagttct ctcagttggg ggcgtagtgt gcgggctcta acacgtccta gtatggtagg
     1741 atgagcaagg tacccgaagc agctccagcc tacacagctg aagcttggcg taatcatggt
     1801 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     1861 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     1921 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     1981 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     2041 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     2101 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2161 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2221 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2281 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2341 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2401 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2461 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2521 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2581 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2641 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2701 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2761 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2821 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2881 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2941 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     3001 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     3061 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     3121 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3181 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3241 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     3301 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3361 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3421 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3481 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3541 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3601 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3661 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3721 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3781 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3841 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3901 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     3961 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
