LOCUS       40924_3861        3831 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pACS-aadA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y, 
            Luzhetskyy A.
  TITLE     From synthetic promoters to new antibiotics: transcriptional 
            activation of silent landomycin A cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y, 
            Luzhetskyy A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2014) AMEG, Helmholtz Institute for Pharmaceutical
            Research Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes, 
            Saarbruecken, Saarland 66123, Germany
REFERENCE   3  (bases 1 to 3831)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3831)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-DEC-2014) AMEG, Helmholtz Institute for Pharmaceutical Research 
            Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes, 
            Saarbruecken, Saarland 66123, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      446..489
                     /gene="A1-14"
                     /regulatory_class="promoter"
     gene            446..489
                     /gene="A1-14"
                     /label=A1-14
     misc_feature    496..546
                     /label=B-CC
                     /note="B-CC"
     CDS             668..1456
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     misc_feature    1469..1518
                     /label=P-GG
                     /note="P-GG"
     regulatory      1519..1559
                     /gene="P21"
                     /regulatory_class="promoter"
     gene            1519..1559
                     /gene="P21"
                     /label=P21
     primer_bind     complement(1610..1626)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1634..1650)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1658..1688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1703..1724)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2012..2600)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2774..3631)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3632..3736)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ccagctgttc cggggatccg
      421 tcgaccctct agaggtaccc tgcagcgcat atcctaacac tccccaaata catcgtcaat
      481 acgacccacc tgcagggagt acgcgcccgg ggagcccggg ggcacgccct ggcacccgca
      541 ccgcgggata aatacctatt gacaattaaa ggctaaaatg ctataattcc accacgctgg
      601 ctacattata cctcggcata gtaggatatc cgccacatta aacaatcaga taaagaggag
      661 gtttcccatg cgcgaggccg tgatcgccga ggtgtccacg cagctcagcg aggtggtcgg
      721 cgtcatcgag cgccacctgg agccgaccct gctggccgtc cacctgtacg gctccgccgt
      781 ggacggtggc ctgaagccgc acagcgacat cgacctgctg gtgaccgtca ccgtccgcct
      841 ggacgaaacc acccgccgcg cgctgatcaa cgacctgctg gaaaccagcg cgtccccagg
      901 tgagagcgag atcctgcgcg cggtcgaggt caccatcgtg gtgcacgacg acatcatccc
      961 gtggcgctac ccggccaagc gcgagttgca gttcggcgaa tggcagcgca acgacatcct
     1021 ggcgggtatc ttcgagccgg cgacgatcga catcgacctg gcgatcctgc tgacgaaggc
     1081 gcgcgagcac agcgtggccc tggtgggccc ggcggccgaa gagctgttcg acccggtgcc
     1141 ggagcaggac ctgttcgagg cgctgaacga aacgctgacc ctgtggaaca gcccgccgga
     1201 ctgggcgggt gacgagcgca acgtggtcct gaccctgagc cgcatctggt actccgcggt
     1261 cacgggcaag atcgccccga aggacgtggc ggccgactgg gcgatggagc gcctgccggc
     1321 gcagtaccag ccggtcatcc tggaagcccg ccaggcctac ctgggccagg aagaggaccg
     1381 cctggccagc cgcgccgacc agttggaaga gttcgtccac tacgtcaagg gcgagatcac
     1441 caaggtcgtc ggtaagtgat aacccatcgt agtgccccaa ctggggtaac ctgggagttc
     1501 tctcagttgg gggcgtagtg tgcgggctct aacacgtcct agtatggtag gatgagcaag
     1561 gtacccgaag cagctccagc ctacacagct gaagcttggc gtaatcatgg tcatagctgt
     1621 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     1681 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1741 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     1801 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     1861 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1921 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1981 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2041 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2101 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2161 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2221 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2281 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2341 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2401 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2461 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2521 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2581 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2641 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2701 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2761 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2821 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2881 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2941 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3001 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3061 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3121 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3181 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3241 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3301 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3361 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3421 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3481 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3541 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3601 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3661 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3721 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     3781 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt c
//
