LOCUS       40924_3856        3800 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pACS-aac, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y, 
            Luzhetskyy A.
  TITLE     From synthetic promoters to new antibiotics: transcriptional 
            activation of silent landomycin A cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y, 
            Luzhetskyy A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2014) AMEG, Helmholtz Institute for Pharmaceutical
            Research Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes, 
            Saarbruecken, Saarland 66123, Germany
REFERENCE   3  (bases 1 to 3800)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3800)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-DEC-2014) AMEG, Helmholtz Institute for Pharmaceutical Research 
            Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes, 
            Saarbruecken, Saarland 66123, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      446..489
                     /gene="A1-14"
                     /regulatory_class="promoter"
     gene            446..489
                     /gene="A1-14"
                     /label=A1-14
     misc_feature    496..546
                     /label=B-CC
                     /note="B-CC"
     RBS             638..646
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             652..1425
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     misc_feature    1438..1487
                     /label=P-GG
                     /note="P-GG"
     regulatory      1488..1528
                     /gene="P21"
                     /regulatory_class="promoter"
     gene            1488..1528
                     /gene="P21"
                     /label=P21
     primer_bind     complement(1579..1595)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1603..1619)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1627..1657)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1672..1693)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1981..2569)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2743..3600)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3601..3705)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ccagctgttc cggggatccg
      421 tcgaccctct agaggtaccc tgcagcgcat atcctaacac tccccaaata catcgtcaat
      481 acgacccacc tgcagggagt acgcgcccgg ggagcccggg ggcacgccct ggcacccgca
      541 ccgcgggata aatggccgtt gacagcccaa gcaaatactc atataatcat agaaaagcgg
      601 ctatatcgct acagcttcca gtaggataca agaagtttaa ggaggtaaca aatgcagtac
      661 gagtggcgca aggcggagct gatcggccag ctcctgaacc tgggtgtcac gccgggtggc
      721 gtgctgctgg tgcacagcag cttccgcagc gtccgcccgc tggaggacgg tccgctgggc
      781 ctgatcgagg ccctgcgcgc ggcgctgggt ccgggtggca cgctggtgat gccgagctgg
      841 tccggcctgg acgacgaacc gttcgacccg gccaccagcc cggtcacccc ggacctgggc
      901 gtggtgagcg acacgttctg gcgcctgccg aacgtcaagc gcagcgcgca cccgttcgcg
      961 ttcgccgccg ccggtccgca ggcggagcag atcatctccg acccgctgcc gctgccgccg
     1021 cacagcccgg cgtccccggt cgcgcgcgtc cacgagctgg acggtcaggt gctgctgctg
     1081 ggcgtcggcc acgacgccaa caccaccctg cacctggccg agctgatggc caaggtcccg
     1141 tacggcgtcc cgcgccattg caccatcctt caggacggca agctggtgcg cgtggactac
     1201 ctggagaacg accactgctg cgagcgcttc gcgctggcgg accgctggct gaaggaaaag
     1261 agcctccaga aggaaggccc ggtgggccac gccttcgccc gcctgatccg ctcccgcgac
     1321 atcgtcgcga ccgccctggg tcaactgggt cgcgacccgc tgatcttcct gcatccgccg
     1381 gaagccggct gcgaagagtg cgacgcggcg cgccagtcca tcggctgata agatatcgta
     1441 gtgccccaac tggggtaacc tgggagttct ctcagttggg ggcgtagtgt gcgggctcta
     1501 acacgtccta gtatggtagg atgagcaagg tacccgaagc agctccagcc tacacagctg
     1561 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     1621 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     1681 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     1741 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     1801 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1861 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1921 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1981 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2041 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2101 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2161 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2221 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2281 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2341 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2401 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2461 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2521 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2581 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2641 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2701 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2761 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2821 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2881 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2941 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3001 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3061 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3121 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3181 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3241 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3301 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3361 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3421 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3481 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3541 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3601 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3661 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3721 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     3781 tatcacgagg ccctttcgtc
//
