LOCUS       V009606                 4018 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009606
VERSION     V009606
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y,
            Luzhetskyy A.
  TITLE     From synthetic promoters to new antibiotics: transcriptional
            activation of silent landomycin A cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y,
            Luzhetskyy A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2014) AMEG, Helmholtz Institute for Pharmaceutical
            Research Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes,
            Saarbruecken, Saarland 66123, Germany
REFERENCE   3  (bases 1 to 4018)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4018)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-DEC-2014) AMEG, Helmholtz Institute for Pharmaceutical Research
            Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes,
            Saarbruecken, Saarland 66123, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      440..480
                     /gene="B6"
                     /regulatory_class="promoter"
     gene            440..480
                     /gene="B6"
                     /label="B6"
     misc_feature    481..530
                     /label="P-GG"
                     /note="P-GG"
     CDS             638..1633
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     misc_feature    1643..1693
                     /label="B-CC"
                     /note="B-CC"
     regulatory      1700..1740
                     /gene="B5"
                     /regulatory_class="promoter"
     gene            1700..1740
                     /gene="B5"
                     /label="B5"
     primer_bind     complement(1797..1813)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1821..1837)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1845..1875)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1890..1911)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2199..2787)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2961..3818)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3819..3923)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ccagctgtgt aggctggagc
      421 tgcttcgggt acctctagag gggctatcct aatcgtgtag gttatatcct agccatttga
      481 ctacgccccc aactgagaga actcccaggt taccccagtt ggggcactac gatgtaaata
      541 cttgacatat cactgtgatt cacatataat atgcgcaggt cggctggttg gctgacaaag
      601 tgcgtaggat gactgtcgag caacgggagg tacggacatg acacaagaat ccctgctcct
      661 tctcgaccgt attgattcgg atgattccta cgcgagcctg cggaacgacc aggaattctg
      721 ggagccgctg gcccgccgag ccctggagga gctcgggctg ccggtgccgc cggtgctgcg
      781 ggtgcccggc gagagcacca accccgtact ggtcggcgag cccggcccgg tgatcaagct
      841 gttcggcgag cactggtgcg gtccggagag cctcgcgtcg gagtcagagg cgtacgcggt
      901 cctggcggac gccccggtgc cggtgccccg cctcctcggc cgcggcgagc tgcggcccgg
      961 caccagagcc tggccgtggc cctacctggt gatgagccgg atgaccggca ccacctggcg
     1021 gtccgcgatg gacggcacga ccgaccggaa cgcgctgctc gccctggccc gcgaactagg
     1081 ccgggtgctc ggccggctgc acagggtgcc gctgaccggg aacaccgtgc tcacccccca
     1141 ttccgaggtc ttcccggaac tgctgcggga acgccgcgcg gcgaccgtcg aggaccaccg
     1201 cgggtggggc tacctctcgc cccggctgct ggaccgcctg gaggactggc tgccggacgt
     1261 ggacacgctg ctggccggcc gcgaaccccg gttcgtccac ggcgacctgc acgggaccaa
     1321 catcttcgtg gacctggccg cgaccgaggt caccgggatc gtcgacttca ccgacgtcta
     1381 tgcgggagac tcccgctaca gcctggtgca actgcatctc aacgccttcc ggggcgaccg
     1441 cgagatcctg gccgcgctgc tcgacggggc gcagtggaag cggaccgagg acttcgcccg
     1501 cgaactgctc gccttcacct tcctgcacga cttcgaggtg ttcgaggaga ccccgctgga
     1561 tctctccggc ttcaccgatc cggaggaact ggcgcagttc ctctgggggc cgccggacac
     1621 cgcccccggc gcctgataca tcccgcggtg cgggtgccag ggcgtgcccc cgggctcccc
     1681 gggcgcgtac tccctgcaga taaaaggctc gttatagcaa ataactatta ggatcatcca
     1741 ctgcagtcta gagggtcgac ggatccccgg aacagctgaa gcttggcgta atcatggtca
     1801 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     1861 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     1921 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1981 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     2041 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2101 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2161 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2221 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2281 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2341 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2401 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2461 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2521 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2581 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2641 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2701 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2761 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2821 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2881 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2941 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3001 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     3061 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3121 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3181 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     3241 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     3301 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     3361 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     3421 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3481 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3541 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3601 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3661 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3721 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3781 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3841 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3901 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     3961 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
