LOCUS       40924_3821        3828 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pACM-aadA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y, 
            Luzhetskyy A.
  TITLE     From synthetic promoters to new antibiotics: transcriptional 
            activation of silent landomycin A cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y, 
            Luzhetskyy A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2014) AMEG, Helmholtz Institute for Pharmaceutical
            Research Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes, 
            Saarbruecken, Saarland 66123, Germany
REFERENCE   3  (bases 1 to 3828)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3828)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-DEC-2014) AMEG, Helmholtz Institute for Pharmaceutical Research 
            Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes, 
            Saarbruecken, Saarland 66123, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      440..480
                     /gene="B6"
                     /regulatory_class="promoter"
     gene            440..480
                     /gene="B6"
                     /label=B6
     misc_feature    481..530
                     /label=P-GG
                     /note="P-GG"
     CDS             652..1440
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     misc_feature    1453..1503
                     /label=B-CC
                     /note="B-CC"
     regulatory      1510..1550
                     /gene="B5"
                     /regulatory_class="promoter"
     gene            1510..1550
                     /gene="B5"
                     /label=B5
     primer_bind     complement(1607..1623)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1631..1647)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1655..1685)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1700..1721)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2009..2597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2771..3628)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3629..3733)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ccagctgtgt aggctggagc
      421 tgcttcgggt acctctagag gggctatcct aatcgtgtag gttatatcct agccatttga
      481 ctacgccccc aactgagaga actcccaggt taccccagtt ggggcactac gataaatacc
      541 tattgacaat taaaggctaa aatgctataa ttccaccacg ctggctacat tatacctcgg
      601 catagtagga tatccgccac attaaacaat cagataaaga ggaggtttcc catgcgcgag
      661 gccgtgatcg ccgaggtgtc cacgcagctc agcgaggtgg tcggcgtcat cgagcgccac
      721 ctggagccga ccctgctggc cgtccacctg tacggctccg ccgtggacgg tggcctgaag
      781 ccgcacagcg acatcgacct gctggtgacc gtcaccgtcc gcctggacga aaccacccgc
      841 cgcgcgctga tcaacgacct gctggaaacc agcgcgtccc caggtgagag cgagatcctg
      901 cgcgcggtcg aggtcaccat cgtggtgcac gacgacatca tcccgtggcg ctacccggcc
      961 aagcgcgagt tgcagttcgg cgaatggcag cgcaacgaca tcctggcggg tatcttcgag
     1021 ccggcgacga tcgacatcga cctggcgatc ctgctgacga aggcgcgcga gcacagcgtg
     1081 gccctggtgg gcccggcggc cgaagagctg ttcgacccgg tgccggagca ggacctgttc
     1141 gaggcgctga acgaaacgct gaccctgtgg aacagcccgc cggactgggc gggtgacgag
     1201 cgcaacgtgg tcctgaccct gagccgcatc tggtactccg cggtcacggg caagatcgcc
     1261 ccgaaggacg tggcggccga ctgggcgatg gagcgcctgc cggcgcagta ccagccggtc
     1321 atcctggaag cccgccaggc ctacctgggc caggaagagg accgcctggc cagccgcgcc
     1381 gaccagttgg aagagttcgt ccactacgtc aagggcgaga tcaccaaggt cgtcggtaag
     1441 tgataaccca tcccgcggtg cgggtgccag ggcgtgcccc cgggctcccc gggcgcgtac
     1501 tccctgcaga taaaaggctc gttatagcaa ataactatta ggatcatcca ctgcagtcta
     1561 gagggtcgac ggatccccgg aacagctgaa gcttggcgta atcatggtca tagctgtttc
     1621 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     1681 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     1741 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     1801 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1861 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1921 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1981 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2041 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2101 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2161 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2221 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2281 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2341 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2401 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2461 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2521 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2581 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2641 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2701 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2761 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2821 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2881 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2941 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3001 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3061 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3121 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3181 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3241 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3301 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3361 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3421 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3481 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3541 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3601 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3661 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3721 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     3781 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
