LOCUS       V009609                 8796 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009609
VERSION     V009609
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8796)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8796)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8796)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     191..341
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     promoter        1053..1256
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    1287..1343
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     CDS             1346..1420
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88136.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     intron          1422..1487
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    1647..1781
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             2070..2489
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(2555..3889)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     rep_origin      complement(6962..7550)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7724..8581)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8582..8686)
                     /label="AmpR promoter"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg
      361 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc
      421 cgggtcaaag ttggcgtttt attattatag tcagtctagg cggccgcgat ctatacattg
      481 aatcaatatt ggcaattagc catattagtc attggttata tagcataaat caatattggc
      541 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt
      601 ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg
      661 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      721 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      781 atagtaacgc caatagggac tttccattga cgtcaataat gacgtatgtt cccatagtaa
      841 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      901 tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta
      961 aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt
     1021 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg
     1081 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     1141 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc
     1201 cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     1261 tagtgaaccg tcagatccgg tcgcgcgaat tcgagctcgg tacccgggga tcctctagag
     1321 tcgacctgca ggcatgcaag cttgggatct ttgtgaagga accttacttc tgtggtgtga
     1381 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     1441 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt cacagtccca
     1501 aggctcattt caggcccctc agtcctcaca gtctgttcat gatcataatc agccatacca
     1561 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     1621 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     1681 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     1741 gtttgtccaa actcatcaat gtatcttatc atgtctggat cgcggccgcc tagaggaagg
     1801 tgctgaggta cgatgagacc cgcaccaggt gcagaccctg cgagtgtggc ggtaaacata
     1861 ttaggaacca gcctgtgatg ctggatgtga ccgaggagct gaggcccgat cacttggtgc
     1921 tggcctgcac ccgcgctgag tttggctcta gcgatgaaga tacagattga ggtactgaaa
     1981 tgtgtgggcg tggcttaagg gtgggaaaga atatataagg tgggggtctt atgtagtttt
     2041 gtatctgttt tgcagcagcc gccgccgcca tgagcaccaa ctcgtttgat ggaagcattg
     2101 tgagctcata tttgacaacg cgcatgcccc catgggccgg ggtgcgtcag aatgtgatgg
     2161 gctccagcat tgatggtcgc cccgtcctgc ccgcaaactc tactaccttg acctacgaga
     2221 ccgtgtctgg aacgccgttg gagactgcag cctccgccgc cgcttcagcc gctgcagcca
     2281 ccgcccgcgg gattgtgact gactttgctt tcctgagccc gcttgcaagc agtgcagctt
     2341 cccgttcatc cgcccgcgat gacaagttga cggctctttt ggcacaattg gattctttga
     2401 cccgggaact taatgtcgtt tctcagcagc tgttggatct gcgccagcag gtttctgccc
     2461 tgaaggcttc ctcccctccc aatgcggttt aaaacataaa taaaaaacca gactctgttt
     2521 ggatttggat caagcaagtg tcttgctgtc tttatttagg ggttttgcgc gcgcggtagg
     2581 cccgggacca gcggtctcgg tcgttgaggg tcctgtgtat tttttccagg acgtggtaaa
     2641 ggtgactctg gatgttcaga tacatgggca taagcccgtc tctggggtgg aggtagcacc
     2701 actgcagagc ttcatgctgc ggggtggtgt tgtagatgat ccagtcgtag caggagcgct
     2761 gggcgtggtg cctaaaaatg tctttcagta gcaagctgat tgccaggggc aggcccttgg
     2821 tgtaagtgtt tacaaagcgg ttaagctggg atgggtgcat acgtggggat atgagatgca
     2881 tcttggactg tatttttagg ttggctatgt tcccagccat atccctccgg ggattcatgt
     2941 tgtgcagaac caccagcaca gtgtatccgg tgcacttggg aaatttgtca tgtagcttag
     3001 aaggaaatgc gtggaagaac ttggagacgc ccttgtgacc tccaagattt tccatgcatt
     3061 cgtccataat gatggcaatg ggcccacggg cggcggcctg ggcgaagata tttctgggat
     3121 cactaacgtc atagttgtgt tccaggatga gatcgtcata ggccattttt acaaagcgcg
     3181 ggcggagggt gccagactgc ggtataatgg ttccatccgg cccaggggcg tagttaccct
     3241 cacagatttg catttcccac gctttgagtt cagatggggg gatcatgtct acctgcgggg
     3301 cgatgaagaa aacggtttcc ggggtagggg agatcagctg ggaagaaagc aggttcctga
     3361 gcagctgcga cttaccgcag ccggtgggcc cgtaaatcac acctattacc gggtgcaact
     3421 ggtagttaag agagctgcag ctgccgtcat ccctgagcag gggggccact tcgttaagca
     3481 tgtccctgac tcgcatgttt tccctgacca aatccgccag aaggcgctcg ccgcccagcg
     3541 atagcagttc ttgcaaggaa gcaaagtttt tcaacggttt gagaccgtcc gccgtaggca
     3601 tgcttttgag cgtttgacca agcagttcca ggcggtccca cagctcggtc acctgctcta
     3661 cggcatctcg atccagcata tctcctcgtt tcgcgggttg gggcggcttt cgctgtacgg
     3721 cagtagtcgg tgctcgtcca gacgggccag ggtcatgtct ttccacgggc gcagggtcct
     3781 cgtcagcgta gtctgggtca cggtgaaggg gtgcgctccg ggctgcgcgc tggccagggt
     3841 gcgcttgagg ctggtcctgc tggtgctgaa gcgctgccgg tcttcgccct gcgcgtcggc
     3901 caggtagcat ttgaccatgg tgtcatagtc cagcccctcc gcggcgtggc ccttggcgcg
     3961 cagcttgccc ttggaggagg cgccgcacga ggggcagtgc agacttttga gggcgtagag
     4021 cttgggcgcg agaaataccg attccgggga gtaggcatcc gcgccgcagg ccccgcagac
     4081 ggtctcgcat tccacgagcc aggtgagctc tggccgttcg gggtcaaaaa ccaggtttcc
     4141 cccatgcttt ttgatgcgtt tcttacctct ggtttccatg agccggtgtc cacgctcggt
     4201 gacgaaaagg ctgtccgtgt ccccgtatac agacttgaga ggcctgtcct cgagcggtgt
     4261 tccgcggtcc tcctcgtata gaaactcgga ccactctgag acaaaggctc gcgtccaggc
     4321 cagcacgaag gaggctaagt gggaggggta gcggtcgttg tccactaggg ggtccactcg
     4381 ctccagggtg tgaagacaca tgtcgccctc ttcggcatca aggaaggtga ttggtttgta
     4441 ggtgtaggcc acgtgaccgg gtgttcctga aggggggcta taaaaggggg tgggggcgcg
     4501 ttcgtcctca ctctcttccg catcgctgtc tgcgagggcc agctgttggg gtgagtactc
     4561 cctctgaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa acgaggagga
     4621 tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcatcca tctggtcaga
     4681 aaagacaatc tttttgttgt caagctagct tctatgggtt taacaatacc tgtgtggaag
     4741 cctggaccga tgtaagggtt cggggctgtg ccttttactg ctgctggaag ggggtggtgt
     4801 gtcgccccaa aagcagggct tcaattaaga aatgcctctt tgaaaggtgt accttgggta
     4861 tcctgtctga gggtaactcc agggtgcgcc acaatgtggc ctccgactgt ggttgcttca
     4921 tgctagtgaa aagcgtggct gtgattaagc ataacatggt atgtggcaac tgcgaggaca
     4981 gggcctctca gatgctgacc tgctcggacg gcaactgtca cctgctgaag accattcacg
     5041 tagccagcca ctctcgcaag gcctggccag tgtttgagca taacatactg acccgctgtt
     5101 ccttgcattt gggtaacagg aggggggtgt tcctacctta ccaatgcaat ttgagtcaca
     5161 ctaagatatt gcttgagccc gagagcatgt ccaaggtgaa cctgaacggg gtgtttgaca
     5221 tgaccatgaa gatctgcgtc tccggtctcg ccgcttagat cgctctgtgt agtagttgta
     5281 gtatatccac tctctcaaag catccaggcg ccccctggct tcgggttcta tgtaaactcc
     5341 ttcatgcgcc gctgccctga taacatccac caccgcagaa taagccacac ccagccaacc
     5401 tacacattcg ttctgcgagt cacacacggg aggagcggga agagctggaa gaaccatgtt
     5461 ttttttttta ttccaaaaga ttatccaaaa cctcaaaatg aagatcctct acgccggacg
     5521 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
     5581 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
     5641 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt
     5701 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga
     5761 gtcgcataag ggagagcgtc gatcgaccga tgcccttgag agccttcaac ccagtcagct
     5821 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
     5881 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc
     5941 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg
     6001 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg
     6061 ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga
     6121 tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg
     6181 ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg
     6241 ctcttaccag cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct
     6301 cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc
     6361 tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg
     6421 gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac
     6481 tgtgaatgcg caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag
     6541 ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct
     6601 cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc
     6661 accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac
     6721 aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgct
     6781 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6841 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6901 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6961 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7021 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7081 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7141 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7201 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7261 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7321 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7381 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     7441 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7501 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7561 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7621 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7681 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7741 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7801 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7861 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7921 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7981 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     8041 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8101 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8161 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8221 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8281 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     8341 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8401 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8461 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8521 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8581 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8641 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8701 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     8761 gtatcacgag gccctttcgt cttcaagaat taattc
//
