LOCUS       40924_3536        7247 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pABcmv-EG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Naik NG, Lo Y-W., Wu T-Y., Lin C-C., Kuo S-C., Chao Y-C.
  TITLE     Baculovirus as an efficient vector for gene delivery into mosquitoes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7247)
  AUTHORS   Naik NG, Lo Y-W., Wu T-Y., Lin C-C., Kuo S-C., Chao Y-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2018) Institute of Molecular Biology, Academia 
            Sinica, Nankang, Taipei 115, Taiwan
REFERENCE   3  (bases 1 to 7247)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7247)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-APR-2018) Institute of Molecular Biology, Academia Sinica, 
            Nankang, Taipei 115, Taiwan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(237..825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(999..1856)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1857..1961)
                     /label=AmpR promoter
     rep_origin      complement(2243..2698)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_recomb     complement(2845..4202)
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        complement(4290..4381)
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     enhancer        4437..4740
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4742..4941
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             5371..6087
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_recomb     complement(6094..6921)
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      661 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1201 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1261 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1321 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1381 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1441 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1501 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1561 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1621 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1681 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1741 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1801 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1861 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1921 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1981 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2041 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     2101 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     2161 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     2221 attgtactga gagtgcacca taaaattgta aacgttaata ttttgttaaa attcgcgtta
     2281 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     2341 aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     2401 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     2461 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta
     2521 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     2581 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     2641 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtac
     2701 tatggttgct ttgacgtatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     2761 gcatcaggcg acgacagcga agtgtctcgc cactacaatg ctgtagtaaa aatgaaaagg
     2821 cttgttatcg gcgtgcggga tcgatccgag cttgagacaa tctctttaca atacaattgc
     2881 ttacattgag cggttgttaa atatcggcac ggtaaacgat agcgaaatta caatgcttat
     2941 agcagatttt tacgatttgt attccaatta taatattgaa ttgccaccac cccaagcgct
     3001 gcctcgaagc agacgacctt ccgttgtgca gccagcggcg cctgcgccgg tgcccacaat
     3061 cgtgcgcgaa caaactaaac cagaacaaat tataccggcg gcaccgccgc caccaccttc
     3121 tcccgtgcct aacattccag cgcctccacc accaccacca ccatcgatgt ctgaattgcc
     3181 gcccgctcca ccaatgccga cggaacctca acccgctgca cctttagacg acagacaaca
     3241 attgttggaa gctattagaa acgaaaaaaa tcgcactcgt ctcagaccgg tcaaaccaaa
     3301 aacggcgccc gaaaccagta caatagttga ggtgccgact gtgttgccta aagagacatt
     3361 tgagcctaaa ccgccgtctg catcaccgcc accacctccg cctccgcctc cgccgccagc
     3421 cccgcctgcg cctccaccga tggtagattt atcatcagct ccaccaccgc cgccattagt
     3481 agatttgccg tctgaaatgt taccaccgcc tgcaccatcg ctttctaacg tgttgtctga
     3541 attaaaatcg ggcacagtta gattgaaacc cgcccaaaaa cgcccgcaat cagaaataat
     3601 tccaaaaagc tcaactacaa atttgatcgc ggacgtgtta gccgacacaa ttaataggcg
     3661 tcgtgtggct atggcaaaat cgtcttcgga agcaacttct aacgacgagg gttgggacga
     3721 cgacgataat cggcctaata aagctaacac gcccgatgtt aaatatgtcc aagctttatt
     3781 taacgtgttt acgtcgagtc aattgtacac taacgacagt gatgaaagaa atacaaaagc
     3841 gcataatatt ttgaacgacg tcgaaccttt attacaaaac aaaacacaaa cgaatatcga
     3901 caaagctaga ttgctgctac aagatttggc aagttttgtg gcgttgagcg aaaatccatt
     3961 agatagtcca gccatcggtt cggaaaaaca acccttgttt gaaactaatc gaaacctatt
     4021 ttacaaatct attgaggatt taatatttaa attcagatat aaagacgctg aaaatcattt
     4081 gattttcgct ctaacatacc accctaaaga ttataaattt aatgaattat taaaatacgt
     4141 acaacaattg tctgtaaatc aacaacgcac agaatctagc gcttaataaa tgtactaata
     4201 acccggatca attaattaag cggccgcccg ggaattcgag ctcggtacca gatcttctag
     4261 attcgaactc gaggcctgca gggatccgta tttataggtt tttttattac aaaactgtta
     4321 cgaaaacagt aaaatactta tttatttgcg agatggttat cattttaatt atctccatga
     4381 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     4441 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     4501 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     4561 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     4621 ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     4681 accttacggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     4741 ctgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
     4801 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     4861 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
     4921 tgggaggtct atataagcag acgtcgttta gtgaaccgtc agatcactag atgctttatt
     4981 gcggtagttt atcacagtta aattgctaac gccagtctcg aacttaacgt gcagaagttg
     5041 gtcgtgaggc actgggcagg taagtatcgg gccctttgtg cggggggagc ggctcggggc
     5101 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
     5161 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
     5221 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattggat
     5281 cggaccgaaa ttaatacgac tcactatggt accggatccc ccgggctgca ggaattcccg
     5341 tcagatccgc tagcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
     5401 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     5461 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     5521 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     5581 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     5641 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     5701 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     5761 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     5821 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     5881 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     5941 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     6001 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     6061 actctcggca tggacgagct gtacaagtaa atcagcaact atatattgat agacatttcc
     6121 agtttgtgat attagtttgt gcgtctcatt acaatggctg ttatttttaa caacaaacaa
     6181 ctgctcgcag acaatagtat agaaaaggga ggtgaactgt ttttgtttaa cggttcgtac
     6241 aacattttgg aaagttatgt taatccggtg ctgctaaaaa atggtgtaat tgaactagaa
     6301 gaagctgcgt actatgccgg caacatattg tacaaaaccg acgatcccaa attcattgat
     6361 tatataaatt taataattaa agcaacacac tccgaagaac taccagaaaa tagcactgtt
     6421 gtaaattaca gaaaaactat gcgcagcggt actatacacc ccattaaaaa agacatatat
     6481 atttatgaca acaaaaaatt tactctatac gatagataca tatatggata cgataataac
     6541 tatgttaatt tttatgagga gaaaaatgaa aaagagaagg aatacgaaga agaagacgac
     6601 aaggcgtcta gtttatgtga aaataaaatt atattgtcgc aaattaactg tgaatcattt
     6661 gaaaatgatt ttaaatatta cctcagcgat tataactacg cgttttcaat tatagataat
     6721 actacaaatg ttcttgttgc gtttggtttg tatcgttaat aaaaaacaaa tttagcattt
     6781 ataattgttt tattattcaa taattacaaa taggattgag acccttgcag ttgccagcaa
     6841 acggacagag cttgtcgaca gttttcattt tttggcagtt tggtggttta tacacatctt
     6901 gggatttgat taaaagatgc atgacttcac ccacacactt ggagtcgtgg ttgtagaaat
     6961 gcgtcagagc cttgattaaa catcggtttt cacacttggc acacgaaacc acgtttgcaa
     7021 tgtaacagtt tagtataaac ctcttcctgt acataccgcc gcgcggtaca ttgttttctt
     7081 tcaaatcgtt caatattttt tttatacacg gaggcatatt aatcttgttg ttcagcatgt
     7141 cgacgacatt tttctttcct tctacgcatt ctttcataca aatgtttctc ttgctgcgtt
     7201 ttttaaaatt ggaattttta atttcattaa tagtgggcgg agccgtt
//
