LOCUS       40924_3531        7082 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pABcir-EG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Naik NG, Lo Y-W., Wu T-Y., Lin C-C., Kuo S-C., Chao Y-C.
  TITLE     Baculovirus as an efficient vector for gene delivery into mosquitoes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7082)
  AUTHORS   Naik NG, Lo Y-W., Wu T-Y., Lin C-C., Kuo S-C., Chao Y-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2018) Institute of Molecular Biology, Academia 
            Sinica, Nankang, Taipei 115, Taiwan
REFERENCE   3  (bases 1 to 7082)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7082)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-APR-2018) Institute of Molecular Biology, Academia Sinica, 
            Nankang, Taipei 115, Taiwan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(237..825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(999..1856)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1857..1961)
                     /label=AmpR promoter
     rep_origin      complement(2243..2698)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_recomb     complement(2845..4202)
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        complement(4290..4381)
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     regulatory      4382..5142
                     /label=cir
                     /note="cir"
                     /regulatory_class="promoter"
     CDS             5206..5922
                     /label=EGFP
                     /note="enhanced GFP"
     misc_recomb     complement(5929..6756)
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      661 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1201 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1261 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1321 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1381 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1441 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1501 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1561 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1621 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1681 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1741 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1801 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1861 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1921 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1981 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2041 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     2101 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     2161 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     2221 attgtactga gagtgcacca taaaattgta aacgttaata ttttgttaaa attcgcgtta
     2281 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     2341 aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     2401 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     2461 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta
     2521 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     2581 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     2641 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtac
     2701 tatggttgct ttgacgtatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     2761 gcatcaggcg acgacagcga agtgtctcgc cactacaatg ctgtagtaaa aatgaaaagg
     2821 cttgttatcg gcgtgcggga tcgatccgag cttgagacaa tctctttaca atacaattgc
     2881 ttacattgag cggttgttaa atatcggcac ggtaaacgat agcgaaatta caatgcttat
     2941 agcagatttt tacgatttgt attccaatta taatattgaa ttgccaccac cccaagcgct
     3001 gcctcgaagc agacgacctt ccgttgtgca gccagcggcg cctgcgccgg tgcccacaat
     3061 cgtgcgcgaa caaactaaac cagaacaaat tataccggcg gcaccgccgc caccaccttc
     3121 tcccgtgcct aacattccag cgcctccacc accaccacca ccatcgatgt ctgaattgcc
     3181 gcccgctcca ccaatgccga cggaacctca acccgctgca cctttagacg acagacaaca
     3241 attgttggaa gctattagaa acgaaaaaaa tcgcactcgt ctcagaccgg tcaaaccaaa
     3301 aacggcgccc gaaaccagta caatagttga ggtgccgact gtgttgccta aagagacatt
     3361 tgagcctaaa ccgccgtctg catcaccgcc accacctccg cctccgcctc cgccgccagc
     3421 cccgcctgcg cctccaccga tggtagattt atcatcagct ccaccaccgc cgccattagt
     3481 agatttgccg tctgaaatgt taccaccgcc tgcaccatcg ctttctaacg tgttgtctga
     3541 attaaaatcg ggcacagtta gattgaaacc cgcccaaaaa cgcccgcaat cagaaataat
     3601 tccaaaaagc tcaactacaa atttgatcgc ggacgtgtta gccgacacaa ttaataggcg
     3661 tcgtgtggct atggcaaaat cgtcttcgga agcaacttct aacgacgagg gttgggacga
     3721 cgacgataat cggcctaata aagctaacac gcccgatgtt aaatatgtcc aagctttatt
     3781 taacgtgttt acgtcgagtc aattgtacac taacgacagt gatgaaagaa atacaaaagc
     3841 gcataatatt ttgaacgacg tcgaaccttt attacaaaac aaaacacaaa cgaatatcga
     3901 caaagctaga ttgctgctac aagatttggc aagttttgtg gcgttgagcg aaaatccatt
     3961 agatagtcca gccatcggtt cggaaaaaca acccttgttt gaaactaatc gaaacctatt
     4021 ttacaaatct attgaggatt taatatttaa attcagatat aaagacgctg aaaatcattt
     4081 gattttcgct ctaacatacc accctaaaga ttataaattt aatgaattat taaaatacgt
     4141 acaacaattg tctgtaaatc aacaacgcac agaatctagc gcttaataaa tgtactaata
     4201 acccggatca attaattaag cggccgcccg ggaattcgag ctcggtacca gatcttctag
     4261 attcgaactc gaggcctgca gggatccgta tttataggtt tttttattac aaaactgtta
     4321 cgaaaacagt aaaatactta tttatttgcg agatggttat cattttaatt atctccatga
     4381 tttaaaacag cctgtgggtt gcacccaccc acagggccca ctgggcgcta gcactctggt
     4441 actgaggtac ctttgtgcgc ctgtttttac tccccttccc ccgaagtaac ttagaagctg
     4501 taaatcaacg atcaatagca ggtgtggcac accagtcata ccttgatcaa gcacttctgt
     4561 ttccccggac tgagtatcaa taggctgctc gcgcggctga aggagaaaac gttcgttacc
     4621 cgaccaacta cttcgagaag cttagtacca ccatgaacga ggcagggtgt ttcgctcagc
     4681 acaaccccag tgtagatcag gctgatgagt cactgcaacc cccatgggcg accatggcag
     4741 tggctgcgtt ggcggcctgc ccatggagaa atccatggga cgctctaatt ctgacatggt
     4801 gtgaagagcc tattgagcta gctggtagtc ctccggcccc tgaatgcggc taatcctaac
     4861 tgcggagcac atgctcacaa accagtgggt ggtgtgtcgt aacgggcaac tctgcagcgg
     4921 aaccgactac tttgggtgtc cgtgtttcct tttattccta tattggctgc ttatggtgac
     4981 aatcaaagag ttgttaccat atagctattg gattggccat ccggtgcaat tcagttaaag
     5041 ctttataact ataagtaagc cgtgccgaaa cgttaatcgg tcgctagttg cgtaacaact
     5101 gttagtttaa ttttccaaaa tttatttttc acaattttta gtggtaccgg atcccccggg
     5161 ctgcaggaat tcccgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
     5221 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     5281 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     5341 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     5401 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     5461 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     5521 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     5581 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     5641 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     5701 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     5761 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     5821 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     5881 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaatcag caactatata
     5941 ttgatagaca tttccagttt gtgatattag tttgtgcgtc tcattacaat ggctgttatt
     6001 tttaacaaca aacaactgct cgcagacaat agtatagaaa agggaggtga actgtttttg
     6061 tttaacggtt cgtacaacat tttggaaagt tatgttaatc cggtgctgct aaaaaatggt
     6121 gtaattgaac tagaagaagc tgcgtactat gccggcaaca tattgtacaa aaccgacgat
     6181 cccaaattca ttgattatat aaatttaata attaaagcaa cacactccga agaactacca
     6241 gaaaatagca ctgttgtaaa ttacagaaaa actatgcgca gcggtactat acaccccatt
     6301 aaaaaagaca tatatattta tgacaacaaa aaatttactc tatacgatag atacatatat
     6361 ggatacgata ataactatgt taatttttat gaggagaaaa atgaaaaaga gaaggaatac
     6421 gaagaagaag acgacaaggc gtctagttta tgtgaaaata aaattatatt gtcgcaaatt
     6481 aactgtgaat catttgaaaa tgattttaaa tattacctca gcgattataa ctacgcgttt
     6541 tcaattatag ataatactac aaatgttctt gttgcgtttg gtttgtatcg ttaataaaaa
     6601 acaaatttag catttataat tgttttatta ttcaataatt acaaatagga ttgagaccct
     6661 tgcagttgcc agcaaacgga cagagcttgt cgacagtttt cattttttgg cagtttggtg
     6721 gtttatacac atcttgggat ttgattaaaa gatgcatgac ttcacccaca cacttggagt
     6781 cgtggttgta gaaatgcgtc agagccttga ttaaacatcg gttttcacac ttggcacacg
     6841 aaaccacgtt tgcaatgtaa cagtttagta taaacctctt cctgtacata ccgccgcgcg
     6901 gtacattgtt ttctttcaaa tcgttcaata ttttttttat acacggaggc atattaatct
     6961 tgttgttcag catgtcgacg acatttttct ttccttctac gcattctttc atacaaatgt
     7021 ttctcttgct gcgtttttta aaattggaat ttttaatttc attaatagtg ggcggagccg
     7081 tt
//
