LOCUS       40924_3381        8181 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pABb1-EG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Naik NG, Lo Y-W., Wu T-Y., Lin C-C., Kuo S-C., Chao Y-C.
  TITLE     Baculovirus as an efficient vector for gene delivery into mosquitoes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8181)
  AUTHORS   Naik NG, Lo Y-W., Wu T-Y., Lin C-C., Kuo S-C., Chao Y-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2018) Institute of Molecular Biology, Academia 
            Sinica, Nankang, Taipei 115, Taiwan
REFERENCE   3  (bases 1 to 8181)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8181)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-APR-2018) Institute of Molecular Biology, Academia Sinica, 
            Nankang, Taipei 115, Taiwan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(237..825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(999..1856)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1857..1961)
                     /label=AmpR promoter
     rep_origin      complement(2243..2698)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_recomb     complement(2845..4202)
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        complement(4290..4381)
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     regulatory      4382..6241
                     /label=b1
                     /note="b1"
                     /regulatory_class="promoter"
     CDS             6305..7021
                     /label=EGFP
                     /note="enhanced GFP"
     misc_recomb     complement(7028..7855)
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      661 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1201 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1261 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1321 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1381 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1441 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1501 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1561 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1621 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1681 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1741 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1801 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1861 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1921 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1981 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2041 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     2101 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     2161 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     2221 attgtactga gagtgcacca taaaattgta aacgttaata ttttgttaaa attcgcgtta
     2281 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     2341 aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     2401 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     2461 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta
     2521 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     2581 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     2641 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtac
     2701 tatggttgct ttgacgtatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     2761 gcatcaggcg acgacagcga agtgtctcgc cactacaatg ctgtagtaaa aatgaaaagg
     2821 cttgttatcg gcgtgcggga tcgatccgag cttgagacaa tctctttaca atacaattgc
     2881 ttacattgag cggttgttaa atatcggcac ggtaaacgat agcgaaatta caatgcttat
     2941 agcagatttt tacgatttgt attccaatta taatattgaa ttgccaccac cccaagcgct
     3001 gcctcgaagc agacgacctt ccgttgtgca gccagcggcg cctgcgccgg tgcccacaat
     3061 cgtgcgcgaa caaactaaac cagaacaaat tataccggcg gcaccgccgc caccaccttc
     3121 tcccgtgcct aacattccag cgcctccacc accaccacca ccatcgatgt ctgaattgcc
     3181 gcccgctcca ccaatgccga cggaacctca acccgctgca cctttagacg acagacaaca
     3241 attgttggaa gctattagaa acgaaaaaaa tcgcactcgt ctcagaccgg tcaaaccaaa
     3301 aacggcgccc gaaaccagta caatagttga ggtgccgact gtgttgccta aagagacatt
     3361 tgagcctaaa ccgccgtctg catcaccgcc accacctccg cctccgcctc cgccgccagc
     3421 cccgcctgcg cctccaccga tggtagattt atcatcagct ccaccaccgc cgccattagt
     3481 agatttgccg tctgaaatgt taccaccgcc tgcaccatcg ctttctaacg tgttgtctga
     3541 attaaaatcg ggcacagtta gattgaaacc cgcccaaaaa cgcccgcaat cagaaataat
     3601 tccaaaaagc tcaactacaa atttgatcgc ggacgtgtta gccgacacaa ttaataggcg
     3661 tcgtgtggct atggcaaaat cgtcttcgga agcaacttct aacgacgagg gttgggacga
     3721 cgacgataat cggcctaata aagctaacac gcccgatgtt aaatatgtcc aagctttatt
     3781 taacgtgttt acgtcgagtc aattgtacac taacgacagt gatgaaagaa atacaaaagc
     3841 gcataatatt ttgaacgacg tcgaaccttt attacaaaac aaaacacaaa cgaatatcga
     3901 caaagctaga ttgctgctac aagatttggc aagttttgtg gcgttgagcg aaaatccatt
     3961 agatagtcca gccatcggtt cggaaaaaca acccttgttt gaaactaatc gaaacctatt
     4021 ttacaaatct attgaggatt taatatttaa attcagatat aaagacgctg aaaatcattt
     4081 gattttcgct ctaacatacc accctaaaga ttataaattt aatgaattat taaaatacgt
     4141 acaacaattg tctgtaaatc aacaacgcac agaatctagc gcttaataaa tgtactaata
     4201 acccggatca attaattaag cggccgcccg ggaattcgag ctcggtacca gatcttctag
     4261 attcgaactc gaggcctgca gggatccgta tttataggtt tttttattac aaaactgtta
     4321 cgaaaacagt aaaatactta tttatttgcg agatggttat cattttaatt atctccatga
     4381 tgcctacact caaatgttca tcaatgggtt atccaacgaa agaaaaaatg aagagaaatc
     4441 agccttacaa gtccatacgc ttgagattcg catgtcgtca acaactattc agcagtaaat
     4501 tttgtcacat aggacttaga tatcagaata tagttttcat atatatgaca aactaattcg
     4561 tgattcatta atttggttaa gcttatttga aacagctgtt ccaacgcaat aaactaacgc
     4621 ggtattctca atccaaacgg tacaccagtt atgtgattcc caatttccca gagcaattgc
     4681 agactcccat agtaaaatgc tcattcattc atcatcacac acgttccgct gaatggtaga
     4741 caacagtggc gccaaaacaa aaacagagcg aggttcataa atggcagctg cgcgctctcg
     4801 actcgacgag cagcgcgagc taagtggaat tcaccgacgg cagccaaact gattagcttc
     4861 taaaaaagtc aaacttttca tattttattg aatggtaacc aacaaaaaga ttggaaataa
     4921 gcaaaaccct tgcaattaaa aattgaaacc acgcacttaa aaataaaaca agcagaataa
     4981 gtaataagct ttgaactata taaaaaacgg ttaaaaacaa ctttaagtaa gtgaatatgt
     5041 atgatttcaa ttatcagctt tcaaatacag taatccgggt caatttgatc actattttat
     5101 aactttttgc tgtgttgtcc ttcggaattc aattattgtc aaataaattt cggcaaaacc
     5161 agaactccat tgatctttat ctgtccatgt aatcgatttt tcatgaaata aaatttaaca
     5221 tactataaaa atagttttaa tatccaaaat tagaatgacg cactggagcg aaattgatca
     5281 tcatataaaa aagcaggttt aagagtgaaa atttccctat ctactaaatt tgggtctcct
     5341 gaatcagagt atgatgtcaa aatcttacaa ctctagtatt tgatcatatt attgcaaaat
     5401 caaactttga aaatctgcct aatacgccta aatataggca atttccctta aaataagcac
     5461 attatttgag aataaacgat tttatgagca aaacactata ctttttatac tatatgatat
     5521 caaaaatgag tttagcagtt catactaaag gtaacacaac attttaaaca cttatagttt
     5581 acatgtaggc atggcaccag tggattgttt agcatcgaca tttccaactg tattgcttac
     5641 accttcaaaa attgtgaata tttataagca aaactgacac ttattaaaat gaaatagttt
     5701 caaatcatca ttaggcacct ataaacttga aaacatggaa tgtgtttggt gccaacaaac
     5761 ccttcagcat ccttgggagg aaatgttttt cggtaccgac ctgatcggat tcgccccagt
     5821 gatcaatttg cccccggtta acggtagcat tttttgttat tcttattttt ttataatgta
     5881 gtttttaatt gcgaattttt cattgcaatt ttttgcgaat ttttcgatga aaaaatattt
     5941 tgaaatgctg tttttttttt taataacaaa tttctactga gctctatttt ttagttattg
     6001 tattgactgc taaaggctaa gcctctgatg gttatccttt tcttagggca tgagaacatt
     6061 tttcccgttc accgatcgtt ggtgcggtga tcgcctgctc ggtgattccc ggtcgtcttt
     6121 ggtcgcgcct ccctttcttt acgcaaataa aggaccaggc aaggactata aaaccaagtc
     6181 actccaagca aatcggttat cagttagcaa acagcgaacc gtaagtcacc tattccagtg
     6241 aggtaccgga tcccccgggc tgcaggaatt cccgtcagat ccgctagcgc taccggtcgc
     6301 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     6361 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     6421 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     6481 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     6541 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     6601 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     6661 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     6721 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     6781 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     6841 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     6901 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     6961 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     7021 gtaaatcagc aactatatat tgatagacat ttccagtttg tgatattagt ttgtgcgtct
     7081 cattacaatg gctgttattt ttaacaacaa acaactgctc gcagacaata gtatagaaaa
     7141 gggaggtgaa ctgtttttgt ttaacggttc gtacaacatt ttggaaagtt atgttaatcc
     7201 ggtgctgcta aaaaatggtg taattgaact agaagaagct gcgtactatg ccggcaacat
     7261 attgtacaaa accgacgatc ccaaattcat tgattatata aatttaataa ttaaagcaac
     7321 acactccgaa gaactaccag aaaatagcac tgttgtaaat tacagaaaaa ctatgcgcag
     7381 cggtactata caccccatta aaaaagacat atatatttat gacaacaaaa aatttactct
     7441 atacgataga tacatatatg gatacgataa taactatgtt aatttttatg aggagaaaaa
     7501 tgaaaaagag aaggaatacg aagaagaaga cgacaaggcg tctagtttat gtgaaaataa
     7561 aattatattg tcgcaaatta actgtgaatc atttgaaaat gattttaaat attacctcag
     7621 cgattataac tacgcgtttt caattataga taatactaca aatgttcttg ttgcgtttgg
     7681 tttgtatcgt taataaaaaa caaatttagc atttataatt gttttattat tcaataatta
     7741 caaataggat tgagaccctt gcagttgcca gcaaacggac agagcttgtc gacagttttc
     7801 attttttggc agtttggtgg tttatacaca tcttgggatt tgattaaaag atgcatgact
     7861 tcacccacac acttggagtc gtggttgtag aaatgcgtca gagccttgat taaacatcgg
     7921 ttttcacact tggcacacga aaccacgttt gcaatgtaac agtttagtat aaacctcttc
     7981 ctgtacatac cgccgcgcgg tacattgttt tctttcaaat cgttcaatat tttttttata
     8041 cacggaggca tattaatctt gttgttcagc atgtcgacga catttttctt tccttctacg
     8101 cattctttca tacaaatgtt tctcttgctg cgttttttaa aattggaatt tttaatttca
     8161 ttaatagtgg gcggagccgt t
//
