LOCUS       40924_3516        6545 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pABCb, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Doehlemann J, Wagner M.
  TITLE     A family of single copy repABC-type shuttle vectors stably 
            maintained in the alpha-proteobacterium Sinorhizobium meliloti
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6545)
  AUTHORS   Doehlemann J, Wagner M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2016) LOEWE-Zentrum fuer Synthetische 
            Mikrobiologie, Philipps-Universitaet Marburg, Hans-Meerwein-Strasse,
            Marburg, Hesse 35043, Germany
REFERENCE   3  (bases 1 to 6545)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6545)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-OCT-2016) LOEWE-Zentrum fuer Synthetische Mikrobiologie, 
            Philipps-Universitaet Marburg, Hans-Meerwein-Strasse, Marburg, Hesse
            35043, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..4492
                     /label=oriVSm part (oriV_p42b)
                     /note="oriVSm part (oriV_p42b)"
     terminator      4515..4542
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(4680..4707)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      4844..4930
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    5014..5043
                     /label=MCS
                     /note="MCS"
     rep_origin      5069..5657
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5684..6472)
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgtcaaaaag gcttttcacg tcaactccaa ccgcaccgtt tctccagcga acgccggccg
       61 atcgtcgatc aggtccttcc ctcgccttgc caatcaaaaa tcttgtaact tcatgatctc
      121 aaaggaagaa tatcctaaca agaagttatc accctgttta tttccgtcac ctttcgttaa
      181 ccatagatcg ccacggacag atgacacgaa atttttcatc cggtcttgcc tctgattggc
      241 gaattggtgc ggacaagcgc catcaacatc ggtccgatgg caaattcgcc tctctcggcg
      301 cggcgggtca ggtcacggag gtaaccaccg gcggaattga tgtgccctgc cctttcaagc
      361 acgcacgcta tgacggtagc cgcattttca ggccccatga cctcgcaggc ctgttcatag
      421 gccgaggggc tcactccgag cattgagcgc acgacgacag ccgccgccat cagatcgcgc
      481 catgtgccca ccgagccctg cggtccatag gcggcgatct ctgggcaggc ctgcagcacc
      541 aggcccagcg ggaaggattt gagcccgccg ccggcaaccg aggctgagtt tgcagctggc
      601 atgggcctgc ccttcccctc ctgtgtcgct attctggccc tcggctcggc ccaaccttcc
      661 gattggtcgt ccaccgttgc gccctgcttc gtttcgaagc gtggttcaag ttcagtagtg
      721 tagtgtgggt ttgaattctg tatgtgccgc tcagaatgtt gggcattgcc gctttgattt
      781 ggagctttaa cctgcatttc caactgattg gtgatttctt cgcgcaacag ttccagttcg
      841 tcgagtgcgg cggcaatctt ctcgaaggat ggcgagcgcg gcagccgttc gacgatgcct
      901 cgaaaatgca ggtggacccc gctccaatca ccagaggcgc cctcttccac ggccatctcg
      961 atgagcttgg cgatgtcgcg cctgcaaagc gtcaggcgct ctttgagccg ctgcagctgc
     1021 aatcgttcgg ctgccatctc ggccgccagc cgctcgatct cgtccgcgcg cgcaagcagc
     1081 ggcgccagcg agaagccgaa tgcctcacgg atctccccgc cctgctcctt gcgagcgtaa
     1141 cgcttgccgt tcgggctgtc cttgcggatc agcaatccgg cctcgatcag cgccgccagg
     1201 tggcgacgga tcgtctgctc cgccattcca tgcgcgcgca gagagagctg cgtattcgaa
     1261 ggaaatacga ccaagccgtt ctcctcggag agctctccct tgggataaaa gctcagcaag
     1321 gcgttcagga tggaaagcgc ccggtctgta atgccgagca gcggcctggc ctcgcacagg
     1381 gcgcgataga gtttccattt atcaatcgat cggccgggct cgatttttcg ggcagcggcc
     1441 tgacttgcca acatgccaaa cgtcatcggc cgccgcccga agggcgtcgt cacacttccg
     1501 ctttccattt tcttcgcctt cttcaaggca aaagatcacg cctcaccaaa ttcggtgcca
     1561 aagactcttg actatgattc gcggaaatgt gattctctgg gtgtctagat cagagagggc
     1621 ttccgcgacg gcaacgttcg ggggcctttt cttttgctta ttgatctccg ttcttcgtca
     1681 aatcctgtgt cttctcgaac gcctcgtaga gacgatccag attgctggcg atgtaggcgc
     1741 caaaggcaga tgccttctcc gccttcagcg cgatggtgaa ctgttttcca tcatccttga
     1801 tcttcgcttt gacggctccg tcccggcgct gccaggcgtg cgccacgggc tgaactgcgg
     1861 cggcgggcga cgcctgttgg ttcctgctga tgaaggcgac aagcatgtcg aagcgggcgt
     1921 ccggctcggc cgtctcgaac tccaccgatc tggtgaactc aatcgcccgg gctgcgatgt
     1981 tttccgtctc gaatttcgcc gaaagatcat gccagcgatc gcggccagtc gtcttggcta
     2041 cgccgatcgc ggccaggact tcggccggaa tccgcttcgt gaccgacagc atcttggaca
     2101 ccgtcgtctt gtctgcgctg agtgccgaca tgatcgtttc ccggtcgaaa cccaacgtgt
     2161 cgagcttgtc ggcaaacatc gtcctctcga tgaaggagag atcggcgcgt gccgaattct
     2221 cctggccctg cgcaatgaca tggtcgcggt cgtcgagtct cttgaccacc gcccggacag
     2281 gtcggccgag ctcctttgcc gcccgcgcac gacgatgacc aaaagcgatc tgatatcgcc
     2341 cctccctctc gggatgcggg cgcacaagga tcggtgaatc ctggccgcgc aggcggatcg
     2401 cctcgactaa ttcccggaac tgttcgtcag tatgcgccag ccggtcggtc acgaaggaat
     2461 cttcgatcag cgccgggtcg agatcgatca ccgtctcgcc gctcgccagc tgctcttcga
     2521 tcgccttggc ggcatcggcc ttggcggcca aggcatcgat gctgcgggtg accgcaccga
     2581 gcgcgccaat ccccttatag gtgatatgct gcctctcgtc gcgaggggcc ggctcatcct
     2641 cattgtttac cgcagtaaac ttcttggaat cgtccatcag gcccgacaga agatttttac
     2701 gcgccatcaa ccagctcctt gccccggttt accgcagtaa acttcccgct catttccggc
     2761 cccatgcctt atggatgaga tcgacgattt ccgtgttgac tgactccatc gcctccatgg
     2821 cgcggtcata ggtcgcgcgg gtaaactgac ttcgctcgac ttcgtacagc gtctggttag
     2881 tcatcgccgc atcggagatc gcgacgctgc gcagcatcgg gttggtgagc acatggctct
     2941 tgaacatcga gcgcatgaag gcgaccatct gcgtctgcgg cccgtcctgc ggatcgtagc
     3001 gggtcacgag gtaacgcagc cagtcgaggt tcatattgcc gccggctccc ttcagcgtgt
     3061 tcagcacttc gccaagcatt tgcaggaact ggcacatcga catgacatcg agcatctgcg
     3121 gatgaacggt gatcagaacc gccgtcgcac cgcaaatggc gctcatggtc aaaaagccaa
     3181 gctggggagg gcaatcgatg atgacgacgt cgtaatcatc cgcgaccgag gccagcgcct
     3241 catccagccg ggcaaagaag acgcgcccat aatcgctcgc tttgccctga gcgaggaccc
     3301 gcggcgtatc gtgctcgaat tccatcagtt cgagattgcc cggcacgaga tgaaggttcg
     3361 ggaagttggt cggccggata atgtcccgca gcggacgccg ctgatcgtca tagcgaatgg
     3421 cggcgtagag tgtctcgttt tcgttgacgt cgaactccgg ctgaaagccg tgtatggcag
     3481 aaagagacgc ttgcggatcg agatcgacgg ccaggacgcg gtgaccggtc agggcgagat
     3541 gctgggcaag atgcgcggca ctcgtcgtct tgccgctgcc ccccttgaag ttgacgacag
     3601 caacgacctg cagatgttcg tggccgcggc gatgacgcac ataatgtgtt ccggcgcgcg
     3661 cattatgctc gagaaagcca cgcatctcct ccatctgctc cgccgtatac gatcggcggc
     3721 cagacggcgt aacctggggg agggcaccct tgccctcgag cgaaagattt cgaagatagc
     3781 cgctggtcac gccgagaaac cgcgccactt cagcaagctg aaattcccgc agacccttta
     3841 gcgcgcgcgg aggaaacatt tcgaggcgat gctgcttcag cttgtcggat agttcctgtg
     3901 cctgaccgat gatcagctgg tcgacatcgg aaattgcttt ttctatttgc ggtgccatat
     3961 tcatggaaat ctcgaaaatg cgatttaaaa gctctacgcg ctttgaaacc gcatttagcg
     4021 ccgattctgg aggggtggca aggcaaatag ggttaacaag atcctatcaa aaggttgtcc
     4081 agtctatttc aagcgcttgc gccggccggg cgtgccgctg gcgggtccag caatcgcaca
     4141 ttcacaggcc gaatcggacg agaccggccg ggaacagcag cagtataatc cgaatcgcaa
     4201 tgtcgggcgt gcaaagcgcc ttgctgcaag cccgcagatg caaggcatcg gttcgctggc
     4261 gtggatggag cggggcgcgg cggtaaggtg ccgggtctgg tcgatcagcc gtaacttgcc
     4321 agatcattcg agcctgagtt cacaggggaa ttgccgcctg gccagctact ccagaggcgg
     4381 acgggtgata ttcgctatcg aaggcaaagc cggaccgctt tccgcctgcg ccatcgcttt
     4441 taccaatgcg atgatcgttt cacgaatatc gggatcggca atcttcagaa aggggaagag
     4501 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttaatt tgatggtcga
     4561 caggggctcg gcgcttagag cctctcttag cgaccgctct agaccgcgac cgcgttcgga
     4621 ctcgcgacgg actacgagta gtagaccctc gcccggaagc aagagtttgt agaaacgcaa
     4681 aaaggccatc cgtcaggatg gccttctgct taatttgatg gcgaggcccc ggagcggacc
     4741 ggcgacgacc gggaggccga ggattacggt ctccttagtc cggcttagga ggagacctcc
     4801 gccgcgctcc tcctcgcgga ggagttcggc gcctagacga catcaaataa aacgaaaggc
     4861 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
     4921 taggacaaat cgggcggctt ctcgccgata atcgggctcg gcctcgccct cgcggagctc
     4981 ctcgattaga ccgtcgttta gaagctcgtc gaggtttaaa cttaattaag cggccgcagt
     5041 actcatatgc ccagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5101 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5161 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5221 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5281 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5341 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5401 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5461 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5521 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5581 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5641 ggcggagcct atggaaaaac gccagcaacg cgcgggatcc ttatttgccg actaccttgg
     5701 tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc
     5761 gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg
     5821 ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta
     5881 ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt
     5941 cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag
     6001 gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg
     6061 cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa
     6121 gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg
     6181 gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc
     6241 caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa
     6301 gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca
     6361 ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc
     6421 caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc atatgttttc
     6481 tcctctgcta cgtaacgcgt ctacttgcgt actagttaac tagttcgatg attaattgtc
     6541 aacag
//
