LOCUS       40924_3511        6743 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pABCa, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Doehlemann J, Wagner M.
  TITLE     A family of single copy repABC-type shuttle vectors stably 
            maintained in the alpha-proteobacterium Sinorhizobium meliloti
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Doehlemann J, Wagner M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2016) LOEWE-Zentrum fuer Synthetische 
            Mikrobiologie, Philipps-Universitaet Marburg, Hans-Meerwein-Strasse,
            Marburg, Hesse 35043, Germany
REFERENCE   3  (bases 1 to 6743)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6743)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-OCT-2016) LOEWE-Zentrum fuer Synthetische Mikrobiologie, 
            Philipps-Universitaet Marburg, Hans-Meerwein-Strasse, Marburg, Hesse
            35043, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..4692
                     /label=oriVSm part (oriV_pMlb)
                     /note="oriVSm part (oriV_pMlb)"
     terminator      4715..4742
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(4880..4907)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      5044..5130
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    5214..5243
                     /label=MCS
                     /note="MCS"
     rep_origin      5320..5865
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(6140..6670)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQARS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 caccgactgc catgtgatct agataatcac ggaccgcggc ggccttgcca tccttcacca
       61 gcgcttcggc agtgcgtccg ccaaatgccg gaagcggctg agcccgatac caggccatcg
      121 cctgttcctt gccgcccgcc caatcgctca cgcggctgat gatctcgagc atttcgcgca
      181 gacgcccctg ggtctttgcc gcagcgctgc gctctgagcg atagagtgtc tcacgcgcaa
      241 gaccggcggt ctgggccaac tgacccttgg acatgccaaa accgtccgcg acctgctcga
      301 tcgcaatctt cccgttccga tccatatagg acaagacgag cggcccattc tgctcggctg
      361 ccttgagtgc atggcccaca gtgttcacca tatcaggagt catgtttctt gtgcaaaaga
      421 tatggcattc ctgaacagct ttcaagcccg ggagcccacc gccggtaggt ggccgcatgc
      481 cgtcacctag agcgccgagc aacctgcccc acgaacctgt acctcaatca tcccgtcatc
      541 ctcgttgccg ggccattggc cttgagcgcg gccatcagca tcggtccgac cgagaactcc
      601 cctgccctcg ccttttccgt cagcacgcgc agatagccac cggccgaatt gatgtgctgt
      661 gcccgttgca gaatacacgc aatcacaatc gcggcgactt cctgacccat cacatggcaa
      721 gcctcctcat aggccgacgg tgaaacccca aggtagccgc gcacctgggc cgcggtgagc
      781 atcaaatcgc gccagctggc gatcccgttg acagcatagc ccgcaaactc ggggcaggcc
      841 ttcagcacca gtccaatcgg atagcttttc aaccgctcgt ccggtgccgg tcttggctca
      901 acggccgccc tgcttttttc gaaagccggt tcaaatacaa aaatggagtc tgtgtttgaa
      961 tcagattgct gccgctcatt ttgggactca ttgccgctag gattcgtgga acttgtgaag
     1021 ctttccagca tcctatccac ttcatcgtgg agcacgacca gctcggcaac gatcccttca
     1081 agctgtgaca gccccgcccg gcgcggaatt gcatccacga tggaccgaaa ccgcctccag
     1141 acgccgcccc agtcgcccgg aaccccttcc tcgatcgccg cctcgaccag cttctgaatg
     1201 tccctgcgat gcagcgtgat gcgctcccgc atcaatttca gcgcctggca ctcggcgcgg
     1261 acctgctcgg cggcgcgctg aaactccgcc gcccgtgcca gcagaggcgc caacgagaac
     1321 ccgaagacct ccatgacagc accaccctgc cccttacgag cgaatctttt gccgttcgga
     1381 ctatcgcggc gaatgatcag gccgcaactc accagctcgg ccaggttcct tctcagcgtc
     1441 gaatcggcca tgccgtgcgc ccgcaaagac agctgtgcgt tcgacgggaa gaccatcaag
     1501 ccgttttcct cgctgatctg gtcgtcagga taaaacgtca acagcccgct cagcaccgcc
     1561 agggcacgat cgccaacgcc gacgatcgac ttgccctcgc atagccaccg gtagacctgc
     1621 catttctcaa cggcggcccc tttcggaatc tgccgcgacg cggcattggc cttcaccaag
     1681 gcaagcgtca tcggccgccg cccaaaaggc gtcgtcgcaa acccactctc catttccctc
     1741 acctttcacg aaggcaaaag aaacctgctc gccgaaatgg cgccacgact cttgacagtg
     1801 attcggggaa gtgcgattct cagcttgcta cagacgagaa gggcttccgc gacggcgacg
     1861 ttcgggggcc tttttctttt gcggtttcag tctcctgttc tcttagacat ttccgattgc
     1921 cggaacgctt cgaaaagctc gtccagccgt tcggtgatga acgcaccgaa cggcgaggct
     1981 tcccttgctt tcagggcaag cgtgaacact tttccggtgt cggtgatctt ggcccgcacc
     2041 gacctgtctt gcggcgccca ggccttttct cgtgtcttcg gcgctgccgg cgaacgtttc
     2101 gccggagcgt tcaggtagtc gaaaagaagc gcaaatcgtg cgtcactgct tggctccgcg
     2161 aaatcagcgc tcttcacata gtcggccgcc ctgcccgcgt tcccaggatg ctccatgagc
     2221 ttggccagtt gccaccacct gtcccgtccg acggccttgg ccgagccaac agccacaatg
     2281 actggctcgg ggatattgcc ggtcacggag cgcatcttcg aaaaggtcgt gtcatcgagc
     2341 gccaaagcgg acttcaccgt gtccgaggtg tggccgcgct tgagaatgcg atcggcaaac
     2401 agggtcctct cgatgaagga cagattggcc ctggcggcat tctcctggcc ctgggcgatg
     2461 acatggtcct cgtcgcccat cttcttgacg acggcgcgga ccggccttcc cagctggcgg
     2521 gcaaccttga cgcggcgatg gccgaatact gtctggaagc gtcctgaaac ctccggatgc
     2581 ggtctgacca gaatcggaga atcttgacct ctctcgcgaa ttgccgacag caactcctga
     2641 tacgcctgat cgtcttcgtc caggcggtcc gagacgaacg aggtgtcaac cagggcagga
     2701 tcgagatcga cgacggaact cttggagagc tcctcgaagg attgcatgat cgagcgggag
     2761 gcaccacgaa cggcataccc cgatacatcc ttgttatcgg ccaccgcagc gacgggtccc
     2821 atcacactgc cgaaaatatc cttacgggcc attcaaccgc ctccgtgagc ggctagcgat
     2881 ctgattccac gagcaaaata gccggttttt actgccgtaa agttcgccgt catgaccgac
     2941 cccacgcacg atggatgagt gtgacgatct cggcgttcac cgcgtcgagc gattccaaag
     3001 cgcggtcata tgtcgagcgg gtgaaggccg atttctccac ctcgtaaagt gtttgcttgg
     3061 tgaggcctgc atcggaaatc gccgtcgact tcaacatcgg gttttccagg atctggccgg
     3121 caagcatgga ctgcatgaag cccaccatct gcgcctgcgg aatatcggtc ggttcgtagc
     3181 gcgtgatcag gtagcgaaac cagtcaagtt cgatcgcggc gccggcggcc ttgatggtct
     3241 tcaatatgcc gccaagcatc agaagaaact ggctcatcga catcaggtcg agcatctgcg
     3301 gatggaccgt aatgagaacc gatgtcgccg ccgacattgc cgtcaaagtc agatagccaa
     3361 gctggggcgg gcaatcgaca acgacgacat cgtaccgatc gtcaacctcc gacaacgctt
     3421 tggccatgcg cgtgaagaac tggcggccgg cactggaggt tttgtcttgc atggcgagcg
     3481 gcgtgtcata ctcgtattcc tgcagctcga gattggcggg aatgatgtcg aggccgggga
     3541 aatttgtcgg tatgatcaca tccgcaatcg atctgcgttc gtcgtcatac ctgatcgctt
     3601 catagagcga gaggttgcga tcgagctcgg gctggaagcc gtgcagagca gacagcgaag
     3661 cttgcggatc aagatcgatc gcaagaaccc gatgaccagt cagcgccagg tgctgggcga
     3721 ggtgggctgc cgtggtcgtc ttgccgctgc cgcctttgaa attgaccacc gcaaccacct
     3781 ggagcttatc gccaagcttg cggtgcggaa cgtacttctt gacttcagtc ctgccatgct
     3841 ggtccaggaa ctgcctgagc tcgagcatct gtgtagccgt gtaggagcga cggccggaag
     3901 gactttggtc aggcaacggc cccttgcctt cgagatggag acgcttcaaa ttgttctgcg
     3961 agaccccgag gtagtgagcg acctccgcca tcgagaatgt gcgtagcgtc ttcgtcgcat
     4021 tgggcggaaa tttctccatc cgaagctggt tcaaccgccg ggaaatctcg gcgccctgtt
     4081 cgaggatttt gtcatcgaac tcgaaacgtg gcagccggat ggagacattc attctcgtcg
     4141 aaacctgaat tggcgctttt tgtaagcaat gcccacaaca agcgccatta agctccgatt
     4201 cggacttacc ggcaagaagt tttgggttaa caaaaggtta acgctggcca gagcgcggcc
     4261 aatcgcactg accgcatcca gaaatttacc gcagtaaaaa aatcgcagaa cgatattgaa
     4321 aacatggatt tgcgcgcacc agacgcgacc attcggcgga gcaaagtttc agatcgtagg
     4381 tacgcggtgg tgttgccaag accgctatgt tgagacgcat gctgacggcc agcatcgcca
     4441 gaccgaggcc aaagactcct gaagacggct cgtcacattt cccggcttca acacatcgtc
     4501 gtgtcaatgc ggatgaaatc tatcggggta ggggccggca atagaccagc ggtaccaacc
     4561 cgtcacgatg cgcaaacagc accgcttgct gacaaagtga aatcgcaagc caatcccgcg
     4621 cggaccgcca gccgggcgac gagggggtgt tccggctgga gattccgcat ggccaaacga
     4681 gtgacgaacg ccgggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     4741 ctgcttaatt tgatggtcga ctcggcggaa agcgagaaga ggttcgcctc ggtctcgccg
     4801 gcggcgaatc cggccgtcgg gccggtcggc cccttccgga agcgaacgcg aaccgtcttc
     4861 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct taatttgatg
     4921 gaactcggag cgtccgcgtc ctcgtaaacg agggcgagcg gccggttaac ggtcgcggag
     4981 tccgaacggt tcttccgagt tcgaatccgc gggtctccgg gagccccgag ataaccggcg
     5041 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     5101 tcggtgaacg ctctcctgag taggacaaat ccgcgatccc gtagccgatc gcgccgaaga
     5161 cctccgcgcc gccggagccc gaactctcgg cgaggaagcc gaccgtcgtc ggggtttaaa
     5221 cttaattaag cggccgcagt actcatatgc ccctacattt gaagagataa attgcactga
     5281 aatctagaaa tattttatct gattaataag atgatcttct tgagatcgtt ttggtctgcg
     5341 cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt cgaaggttct
     5401 ctgagctacc aactctttga accgaggtaa ctggcttgga ggagcgcagt caccaaaact
     5461 tgtcctttca gtttagcctt aaccggcgca tgacttcaag actaactcct ctaaatcaat
     5521 taccagtggc tgctgccagt ggtgcttttg catgtctttc cgggttggac tcaagacgat
     5581 agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct
     5641 tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat
     5701 aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg
     5761 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag
     5821 cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg
     5881 gccctctcac ttccctgtta agtatcttcc tggcatcttc caggaaatct ccgccccgtt
     5941 cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg
     6001 aagcggaata tatcctgtat cacatattct gctgacgcac cggtgcagcc ttttttctcc
     6061 tgccacatga agcacttcac tgacaccctc atcagtgcca acatagtaag ccagtataca
     6121 ctccgctacg ggatccttag gtggcggtac ttgggtcgat atcaaagtgc atcacttctt
     6181 cccgtatgcc caactttgta tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca
     6241 cgtagatcac ataagcacca agcgcgttgg cctcatgctt gaggagattg atgagcgcgg
     6301 tggcaatgcc ttgcctccgg tgctcgccgg agactgcgag atcatagata tagatctcac
     6361 tacgcgcctg ctcaaacttt ggcagaacgt aagccgcgag agcgccaaca accgcttctt
     6421 ggtcgaaggc agcaagcgcg atgaatgtct tactacggag caagttcccg aggtaatcgg
     6481 agtccggctg atgttgggag taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa
     6541 gagcagccct catggatttg acttggtcag ggccgagcct acatgtgcga atgatgccca
     6601 tacttgagcc acctaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc
     6661 tgcgtaacat atgttttctc ctctgctacg taacgcgtct acttgcgtac tagttaacta
     6721 gttcgatgat taattgtcaa cag
//
