LOCUS       40924_3506        7320 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pABC6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Doehlemann J, Wagner M.
  TITLE     A family of single copy repABC-type shuttle vectors stably 
            maintained in the alpha-proteobacterium Sinorhizobium meliloti
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   Doehlemann J, Wagner M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2016) LOEWE-Zentrum fuer Synthetische 
            Mikrobiologie, Philipps-Universitaet Marburg, Hans-Meerwein-Strasse,
            Marburg, Hesse 35043, Germany
REFERENCE   3  (bases 1 to 7320)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7320)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-OCT-2016) LOEWE-Zentrum fuer Synthetische Mikrobiologie, 
            Philipps-Universitaet Marburg, Hans-Meerwein-Strasse, Marburg, Hesse
            35043, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..5009
                     /label=oriVSm part (oriV_p42f2) derived from pLoriV-42f2
                     /note="oriVSm part (oriV_p42f2) derived from pLoriV-42f2"
     terminator      5065..5092
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      5230..5257
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      5394..5480
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    5564..5593
                     /label=MCS
                     /note="MCS"
     terminator      complement(5625..5652)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      5745..6333
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6426..7214)
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gaaactgtca ctggtccgtt gatacaatcc agtgtgatca ttcactcacc tcgtcctcgg
       61 gacccgcgga tcgagatgct gagtagcctg cgtctgcctt tcctggttca cggccggtcg
      121 gagacggagc acgttcacgc ctggcttgat atcgataatg aaaatgcggt gcgcaggtcc
      181 accgaacacc tgctcgatct cgggcaccgc cgcatcgcca tgatcaatgg cctcaggggc
      241 aagacttatt cgatccatcg cgaacagggt tttcggctcg cccatcagga gcgtggactc
      301 atcgccgacc ccaacatgat gatctgcgat aaattctccg acgagagcgg ctttcgccat
      361 gcccgctcct tcctcgagag agcaaatgca ccgaccgcca tcgtcgccgg ttccacgatg
      421 acggcgcttg gggtttaccg cgccgtccgc tcgctcggga tgacggtggg acaggatgtt
      481 tccgtcatcg cccatgacga cgtcttccct tatctgacgg ccgaaaacat ggtgccgtcg
      541 ctctcgacaa cccgatcttc catgcgtgct gcgggcacac gctgcgccga actgacgctg
      601 cagctgatcg ccggacgcgc cgctgaggag gtccatgaat tatggccggt cgagctgatc
      661 ctacgagaaa gcactgcgcc gccgcgctga ctgggcggct cctgtcgccg agacctttcg
      721 tccagccgcc aatcggactg gaagcaggtg agtttaccag atgaggcaac ggcgcaactg
      781 cgctgtagct ggggccttta tccggcatgg cgcttcgccc caccattcgc cctgacgagc
      841 gccatcagca tcgggccaat cgcgaattcg cccctttcgg tcctgcgggt gagatcgcgg
      901 agatagccgc cgggcgagtt gatgtgtcct ccccgttcca agatgcaggc gatcacggtc
      961 gcggcgtttt ccggccccat gccggcgcag gcttcctcat aggccgaggg gctgacgccg
     1021 agcatcgagc gcacgacgac ggcggccgac atcaggtcgc gccaattggc gatcgcgcca
     1081 cccggcccat aatcgagaat ggcggggcag gcctgcagga caaggccgag cgggaaggat
     1141 ttcaggctgt ggccggcgat aggattggcg gcattcggcg ccttgccacc agatccggca
     1201 gaaggcctcg gcaatttcag cctttgcgaa tccgacggct cggccacccc ttcattgttt
     1261 gccgctgcct tttcgccctg cttcgtgtcg aagcttggtt caagttcaat agtggagtcg
     1321 gggtttgaat tctgtttgtg gcgctcaatt tgagattcat tggcgtccaa tttttctctt
     1381 tttatctgtc tttccagcag gttgacggcc tcgtcgcgca gcagccccat ttcttcgagc
     1441 agcgatgcca gttcttcggt gctcgccacc cgcgggatcc gtgcgacaag agcacgaaac
     1501 atcaccgtca tctgctccca gtcaccggga accgcttcgt cgatggccgc cgaaatcagc
     1561 ttggaaatat cgcgccggca gatcgtcagg cgttctctgg tcgcccggag cagttcccgg
     1621 tcggcaactg cctgggctgc cagattttca atctcgaccg cacgggccag aagcggcgcg
     1681 atgctgaagc cgtaagcttc gccgatggcc cctgccctgt cgcggcgggc atagcgtttc
     1741 ccgttcggac tgtccttgcg caagatcagg ccagcctcga ccaggacggc caagtgccgc
     1801 cgcagcgtcg ccggcgtcat gccgcgggcg cgcagcgata gctgtgcatt cgagggaaag
     1861 acgatcaggc ccttctcctc ggaaatttca tcgtcgggat aaaaggtcag cagcgcatca
     1921 agcaccgtca gcgcccgatc ggtcacgccg agcgccggtc gcgcctcgca aattgcccga
     1981 aacagcttcc acttgctgcg catcatcccc ggctcgatcg tcccggcctg ctgctggctt
     2041 gccagcatgc caagcgacat cggccgccgc ccgaagggcg tcgtcacata accactctcc
     2101 attgccttca ccttcgttca ggcaaaagaa aaccatccac caatttggtg cctaagacgc
     2161 ttgactgtga ttcgtggaaa tgcgattctc gatgtgctta agatctgaga agggcttcca
     2221 cgacggcgtc gttgggggcc tttttctttt gcgcgctacg ctcctctgtg cttcttgaat
     2281 tcctggaaca ggccctggag ccgttcctgg acgaatcgat cgaaccccgg cgccgtcttg
     2341 ccgtcgaaga cgaaagtagc gccggctgct gtctttttga tcatcacagg cacgccgttg
     2401 acttcggttt tggccaccgc cggctttggc gcggccgtct tcttcgtcgc cagaaccaat
     2461 gcccggtgga agcgttcgtc gctcgaaatc ctgcgtgcgt cgtccgcgga caactccgcg
     2521 atgatcgtcc ggacgtcggc tttctccagc cgatcgccca gctccagcca gcggcggcgg
     2581 ccgatctcgg gcgcggcacc gatggcgttg atgaggtcga gcggcacctg ccgcatgacg
     2641 atcagcattc tcgacagcgc cgccttgtcg acactgagcg ccgccatgat gacatctcgg
     2701 ccgaagccgc gttcctcgag ccgcgacgcg aagagcgcgc gctcgatata ggagagattg
     2761 gtacgggcgc tgttttcctg tccctggctg acgaccagct ggtcatccgt gagatcgcgc
     2821 acgaccgcac gaaccttgat gccgatgtcc ctgatggccg cgagccgtcg atggccgtag
     2881 gcgacctgat aacgaccctt ggcttcagga tgcggacgca cgaggatcgg aacctgctgc
     2941 ccgtgttcgc ggatctgctc gacgagatcg gccagttcgg cagcgtcgat cgccaggcgg
     3001 tcgctgacga aggaggcgtc gatcaggctt gggtcgagtt cgatgatcat ctggcccgcc
     3061 gcgagctgcc gttccagctc acccgcccgc tccatcttct cggtaatgtt gccgagcgtc
     3121 ttcgtaatgc cgccgaccgg cacgccgaca cgttcctgcg gcacgaaccc ggcgatcggg
     3181 cgattgtcgc ggggggcgat cgcttccacc cttgccgcgc tcggagcgga gatttcgatg
     3241 aggttcttgc gcgccatttc tatctccttc cccaggtgga gcgaatatgc gcttcgatct
     3301 cgccattcac gaggttcagc gactccagcg cccgatcata agttccgcgc gtgaactgcg
     3361 accgctcgac ttcgtagagt gtctgcttgg tgacgccggc atcggaaacg gccgtcgatt
     3421 tcaccatggc attgtggagc atgcgattgc cgaagatcgc ccgcatgaag ccggtcatct
     3481 ggctctgcgg cccgtcattc ggctcgaacc gggtcacgag atagcgcatc cagtcatagc
     3541 tggtgcgccc gccggccctc tcgacgaccg acatcagttc gctcgtcatc gtcaggaact
     3601 gcgacatcga catgacgtcg agcatctgcg ggtgaacggt gatcaacacc gaagtcgccg
     3661 cgcagagcgc cgacatcgtc aggaagccga gctgcggcgg gcagtcgacg acgacgatgt
     3721 cgtagaggct ttcgatatcg gtcagcacct cgccgatacg ggcaaagaac atcgtctccg
     3781 ccgtgcccga tgccatggcc ttcggcgtct cgtgctcgaa ttccatcaat tccagattgc
     3841 cgggaatgag atgcaggttc ggcgtatagg tggcgcgcac gatatcggcg atcgggcgcg
     3901 gatcgtcata gcggattgcg ccgtagatcg tttcgccctc gccgacgtcg aattccggct
     3961 gatggccgaa cagcgcggac aaggaggcct gcgggtcgag gtcgacggca agcacgcggt
     4021 agccccggag cgccatgaac tgcgcaaaat gggccgcggt cgtggtcttg cccgacccac
     4081 ccttgaaatt catcacggag acgatctgga gcttttcgcc cggcctgcgg gccggcacat
     4141 atttcggcgt tccgttccgc tcgtcgagga cgcggcggat ccgatccatg tcggttgcgc
     4201 tatagagccg tcgtccgttt gccagcggat ccggaccatg gccgtcggcc gccacctgcc
     4261 ggagataacc ttcgccgatg ccgatgaagg ccgcggcctc tgccggagaa aatgtcctga
     4321 tcgtcttttg cgatagcgga ggaaagattt tggcctgatg ctgctgaagt tgataggaca
     4381 actccttcgc atccgttgcc agaagcgacg gaaggtgctc ttgatcatct tgaagagcaa
     4441 gactcggctg catgatctct ttcctgcgta actgcgcaat tttgaacgaa agcgcttcag
     4501 ttgcgcagtt actatatgcc gcgattcgtg accgaattac aaatgcttaa ccagaacgcg
     4561 catcagagcc ggcaaaacgg gctccggaaa ccggaggttt gacggattga acggcggtga
     4621 ttcgcaccgc gcaagatgcc gacaggcaag gcctcgattc tcattataca tgtgaaattc
     4681 attgcgattt tgcggcacag cggcacagac aggctcaagg taaccatggc ccgtcatcct
     4741 tcaggtgttg tcgccgttgg tcatcacgac ccgtcaaaac cctttgttcg gttcgagaat
     4801 gcttaggctg cggttcgtac ggcagccgag caatttcacc gcggcgatgc gcgatcgctt
     4861 ccgtccaaat agtgtcagcc actgccgtct ttcggtgaca tgccgatggt catcgcacta
     4921 tgccctatga gcagccgaca agccgcctgc ctgtttctta gtcccgatta gcaaccacta
     4981 acttctcgtg gaactctatc gtaactgaag tctattgaag gaacactgta tctcggtcaa
     5041 gtgggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttaatt
     5101 tgatggtcga caggggctcg gcgcttagag cctctcttag cgaccgctct agaccgcgac
     5161 cgcgttcgga ctcgcgacgg actacgagta gtagaccctc gcccggaagc aagagtttgt
     5221 agaaacgcaa aaaggccatc cgtcaggatg gccttctgct taatttgatg gcgaggcccc
     5281 ggagcggacc ggcgacgacc gggaggccga ggattacggt ctccttagtc cggcttagga
     5341 ggagacctcc gccgcgctcc tcctcgcgga ggagttcggc gcctagacga catcaaataa
     5401 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     5461 ctctcctgag taggacaaat cgggcggctt ctcgccgata atcgggctcg gcctcgccct
     5521 cgcggagctc ctcgattaga ccgtcgttta gaagctcgtc gaggtttaaa cttaattaag
     5581 cggccgcagt actcatatgc ccactaagag tttgtagaaa cgcaaaaagg ccatccgtca
     5641 ggatggcctt ctgcttaatt tgatggttat cagaagagtg taagcgggtt gactatacta
     5701 ctgtttcaga ctggcgtaat cactcagtag atcaaaggat cttcttgaga tccttttttt
     5761 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5821 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5881 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5941 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6001 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6061 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6121 tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6181 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6241 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6301 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcgac tggcaatcac
     6361 tgagtaagac tattctgtcc agaccttttc tccgacgaat agagtaacag aagtcgggat
     6421 ccttatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac
     6481 tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca
     6541 agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc
     6601 ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca
     6661 tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc
     6721 gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc
     6781 aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg
     6841 gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg
     6901 cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca
     6961 gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa
     7021 gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcactg
     7081 tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt
     7141 tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc
     7201 accgcttccc tcatatgttt tctcctctgc tacgtaacgc gtctacttgc gtactagtta
     7261 actagttcga tgattaattg tcaacagact gattggtcag gcacaacttt cagataagac
//
