LOCUS       40924_3501        7181 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pABC5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Doehlemann J, Wagner M.
  TITLE     A family of single copy repABC-type shuttle vectors stably 
            maintained in the alpha-proteobacterium Sinorhizobium meliloti
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Doehlemann J, Wagner M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2016) LOEWE-Zentrum fuer Synthetische 
            Mikrobiologie, Philipps-Universitaet Marburg, Hans-Meerwein-Strasse,
            Marburg, Hesse 35043, Germany
REFERENCE   3  (bases 1 to 7181)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7181)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-OCT-2016) LOEWE-Zentrum fuer Synthetische Mikrobiologie, 
            Philipps-Universitaet Marburg, Hans-Meerwein-Strasse, Marburg, Hesse
            35043, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..4870
                     /label=oriVSm part (oriV_p42f1) derived from pLoriV-42f1
                     /note="oriVSm part (oriV_p42f1) derived from pLoriV-42f1"
     terminator      4926..4953
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      5091..5118
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      5255..5341
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    5425..5454
                     /label=MCS
                     /note="MCS"
     terminator      complement(5486..5513)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      5606..6194
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6287..7075)
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gaaactgtca ctggtccgtt gatacaatcc agtcatcaca gtcggccttt atggaggatg
       61 catggacaaa ccctggaaga tcagccgcgg gccgattgcg gcgaccgagc tcgacgtgga
      121 gaaggccaac gcgatcaaca gcatgttgat ccggccggtc ggcgtgcttc cggccaagcc
      181 cggcgatccc gttcttccct tcgccgtcgg cctcttcaac gagctgcgtc cgctcttgaa
      241 gcccgaggcc ggtgtaacga cgctccggcg cgcgaccgct gcctttgtcc attgccggcg
      301 ctattatttc gccagcgccc agcccgattc catgcgccac aacatcgacg gcgaaccggt
      361 cgaacccttg tcggccgaag accggctggt cgcgcagaag cgcttcctga gcctcaaaca
      421 gagcgccaag gtcgaggcgc ccgagccggc actggttccc acgcccgtgc tcagcaagaa
      481 cgaacagatc cgcgccgctc ttctgcgcgg cagaaagagc accgtcagct aacttcggcg
      541 attggcggat ccttcgcccg caacagcgcc gtcagcatcg ggccgggcgc gaattcgccg
      601 cgttcggcgc gacgcgtgag atccctgaga tagccgccgg cggaggtgat gcaccctccc
      661 ctttgcagga tgcaggccat gacggtggcg gcattttccg gccccatcac cgcacaggct
      721 tcctcgtagg ccggcgggcc gacacgcagc atcgtgcgca cggtgaccgc cgccgtcatg
      781 agatcccgcc agctgccgat cgctccgccc gggccataat cggcgatatc ggggcaagcc
      841 ttcaggaccg tgccgagctg gaaggatttc aatctccctg catccgtccg aacagcccgc
      901 aatccctgcc cgccatcccc tcgcctgcct tgatcatcgt ctttcagccc tgacctcggt
      961 tgatcgtcca gcctcgcctc ctgcgccgtt tccaggcccg gttcagattc agatagggac
     1021 tcgggattcg aactctgtat gtggcgctca aactgggatt cattggtgct cgcattttga
     1081 attttctctc gcgattccaa tgtgttgatg atggcgaggc ggagcatttc cagctccttt
     1141 agaagcggcc gcagattttc gggtgttgcc gccttcggga tgcgttccag cagcgcgtga
     1201 aacagcgaag aaattcgctc ccactcgccg ggaatggctt cctcgacggc ggcggcgatg
     1261 agcttggcga tgtcgcgccg gcagatcgtc agccgctcct tcatcgcccg cagcatctgt
     1321 cggtcagcgg cgagcccggc ggccattgcc tcgatctccg aggcgcgggc cagaagcggg
     1381 gcgagactga agccataggc ctcgtcaatg acgcctgccc tgccgcggcg gacataacgc
     1441 ttgccgttcg ggctgtcctt gcgggagatc aggccggcgt cgacgagcac ggccagatgc
     1501 cggcggagcg tcgcggcggc catgccgcgt gctcgcagcg aaagctgcgc attcgaggga
     1561 aagaccacca gccctttttc gctggagagt tcgtcctcag gatagaagct cagcagcgca
     1621 tcgagcacgg tgagcgcccg gtcggtcacg ccgagccctg cccgcgcctc gcagaccgat
     1681 cggaacaatt tccatttgtt gcgcgtcgtc ccggccgcaa tcgcttcgcc gcgccgctgt
     1741 ttcagcagca gggcaagcga catcggccgc cgcccgaagg gcgtcgtcac agattcactc
     1801 tccatttcct tcacctctat tcaggcaaaa gaaattcggt caccgaaacg atgcgaggag
     1861 gaaaagcgct gcagcgtcat tgccacgtct gaaaagacgc gagggcgctt caggactctt
     1921 gactgggatt cgtgaaaatg cgattctctg tcttgcttaa ggatgtgaga agggcttcca
     1981 cgactcgttc gtttgggggc ctttttcttt tgcggtttca gtctccttgt ttgctctcct
     2041 cggctaggaa ttgctcatag agcgcatcca gcctgccgga gagaaattca ccgaagcggc
     2101 cggcattcct cgccttcatc gagagcgaga acgccttgcc gtcattcttc atttccgcct
     2161 ggacagcctt atcaagcggc tgccatgtca ccttcctcgg ggcgacggtt ttcttgaccg
     2221 gccgcgcgct tttgttcagc gccgcataga gcgcttcgaa acgtttgtcg gaaggtaact
     2281 gatgaaagtt gtcgccttcg aggaccttgg cgacattcgc cgcattggcg gccttgccgg
     2341 cgagcagcga cagttccacc cagcgctcgc gcccgacccc gggtgcggag ccgatcgcct
     2401 ggacgacctc acgcgacagg cgctccatca ccgacatcat cttcgagacc gaagccgcat
     2461 tggcggcgag cgccgtcgag atcacctccc tgtcgtagcc gagctcctcc agccgcttgg
     2521 cgaagagcgc ccgctcgata aaggtgaggt cggcgcgcgc cgaattctcc tgaccctggg
     2581 cgatgacatg ggtccggtcg tcgatcgcct tgacgacggc gcggacattg cggccgagct
     2641 cgcgggcggc gcgcagccgc cgatggccga agaccacctg atagcgtccg tcgatcctgg
     2701 cgtggggacg caccaggatg ggcgtgtcct gcccgcgtgc gcggatcgcc tcgaccagcg
     2761 tgcggaacgc ctcgtcatcc tccgacaaac gatccttgac gaacgaggtt tcaacaagct
     2821 cggggtcgag gtcgaccact gcctcgccct ccagaaattt ttccgcctgc ttggcgagct
     2881 catccagcga gcggacgagc gaccggcctg ccccgcgcat cggatagttc ggcgcgcccg
     2941 cgttttcgtc cggcatctcg gccagccctt ccagcagatt ctttcgtgcc atcgcttcct
     3001 ccgattgcgg tcattccatt gatttgcttc acggaaacgg cgaatttgtc agccgacaaa
     3061 ctcaccgccc ccatgcctgg tgaataaggc cggcgatctc cgcgttgacg gcgcccatgg
     3121 cctccagcgc gcgatcatag gttccccggt tcatcgaggc gcgctcgacc tcgtaaagcg
     3181 tctgcttggt gatcccggca tccgagatcg cggtggattt gaccatctgg ttcttcagca
     3241 tgaattcgtg gaacatcgtc gacatgaagc cgaccatctg cgcctgcggc acgtcggtcg
     3301 gctcatagcg ggtaatgaga tagcggtacc aggagaggtt cacttcggcg ccggcggcac
     3361 ggatcggctt caggatgccg ccgagcatga gcaggaactg ccccatcgac atgacgtcga
     3421 gcatctgcgg atggatggtg atcaaaacgc tggtcgccgc cgtcagcgcc gtcagcgtga
     3481 ggtagccgag ctgcggcggg caatcgatga cgacgacgtc gtagcggtca tcgacctcag
     3541 cgagcgcgcg cgagatacgg gtgaagaagg tcttgccgtc attgctcgat ttgtcggcca
     3601 tggcgagcgg cgtgtcgtat tcatattcct gcaagtcgag attggccggc acgatatcga
     3661 ggccgggaaa attcgtcctg tggatgatct cagagagcgg cttcctcttg ccgtcatagc
     3721 ggatcgcctc atagagcgag gaggcctggt cgaattccgg ctggaagccg tgcagggaag
     3781 agagggacgc ctgcggatcg agatcgatgg caagcacgcg atgaccggtc agcgccagat
     3841 gctgggcgag atgggctgat gtcgtcgtct tgcccgagcc gcccttgaaa ttgacgacgg
     3901 cgatgacctg cagcttctca tggccgcggc gatggggcac atacatgcgg ctctcggagc
     3961 gaccatgcgc atcgagatag cgcctgagct ccagcatctg ctcggcgcta taggagcgcc
     4021 gcccggaagg tgaggtctgc gggcaggggc ctttgccttc gagatgcagt ttcttcagcg
     4081 tgctctgcga cgccccgaca taattggcga cctcggcgag cgagaagctg cgcagcgtct
     4141 tctttgcgtt cggcggaaag cgctgcacgc tcaagagatg cagcttcttc gaaatcacat
     4201 cgccgtgctc gagaatctga tcctcgaaat gcagcggcgt agccttcaag gcgttcgaat
     4261 tagcgttcat cggtagcaat ctcttggata acgattttcg ggatcaaaag cccctataac
     4321 cgttattatc cgcgattctc cgatgcccgc caagaaattt aaggttaacg catattaacc
     4381 ttttcggtgc tcgttcaagc acttggcgtc cttcacatgt gcatggaaga gccgggaact
     4441 tcgggccggg ggcgcggcgg acgcgccccc ggcggcgccc ggccgaaggg gaggggacat
     4501 tcggcgggct taacttagtg gcgcttcgcc tgtcgtgatg cgctgttcgt cctcgccgaa
     4561 caggtgaacg gtgtcatgaa caggggcgat cctcagcacg tcgccgggag cggctctgat
     4621 ccgctcgcgg aagacgcagg tgagcgtctg ggtgcccagc cggacgatga caagggtttc
     4681 cgagccggtc ggctcgacga cgacagtcgt cacctcgatg ccgtcagggt ccattcggat
     4741 atgttcggga cgaacgccgt aagtcacggc gtccctgggg tgacgctcgc tcggcaggag
     4801 caggccgtcc tcggtccgaa agccattttc cgtcatcact aacttctcgt ggaactctat
     4861 cgtaactgaa gtctattgaa ggaacactgt atctcggtca agtgggaaga gtttgtagaa
     4921 acgcaaaaag gccatccgtc aggatggcct tctgcttaat ttgatggtcg acaggggctc
     4981 ggcgcttaga gcctctctta gcgaccgctc tagaccgcga ccgcgttcgg actcgcgacg
     5041 gactacgagt agtagaccct cgcccggaag caagagtttg tagaaacgca aaaaggccat
     5101 ccgtcaggat ggccttctgc ttaatttgat ggcgaggccc cggagcggac cggcgacgac
     5161 cgggaggccg aggattacgg tctccttagt ccggcttagg aggagacctc cgccgcgctc
     5221 ctcctcgcgg aggagttcgg cgcctagacg acatcaaata aaacgaaagg ctcagtcgaa
     5281 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     5341 tcgggcggct tctcgccgat aatcgggctc ggcctcgccc tcgcggagct cctcgattag
     5401 accgtcgttt agaagctcgt cgaggtttaa acttaattaa gcggccgcag tactcatatg
     5461 cccactaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttaat
     5521 ttgatggtta tcagaagagt gtaagcgggt tgactatact actgtttcag actggcgtaa
     5581 tcactcagta gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5641 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5701 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     5761 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5821 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5881 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5941 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt
     6001 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6061 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6121 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6181 ggagcctatg gaaaaacgcc agcaacgcga ctggcaatca ctgagtaaga ctattctgtc
     6241 cagacctttt ctccgacgaa tagagtaaca gaagtcggga tccttatttg ccgactacct
     6301 tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca
     6361 agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact
     6421 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg
     6481 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc
     6541 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt
     6601 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc
     6661 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg
     6721 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc
     6781 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct
     6841 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat
     6901 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat
     6961 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga
     7021 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatatgtt
     7081 ttctcctctg ctacgtaacg cgtctacttg cgtactagtt aactagttcg atgattaatt
     7141 gtcaacagac tgattggtca ggcacaactt tcagataaga c
//
