LOCUS       V009627                 6954 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009627
VERSION     V009627
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Lamping E, Monk BC, Niimi K, Holmes AR, Tsao S, Tanabe K, Niimi M,
            Uehara Y, Cannon RD.
  TITLE     Characterization of three classes of membrane proteins involved in
            fungal azole resistance by functional hyperexpression in
            Saccharomyces cerevisiae
  JOURNAL   Eukaryotic Cell 6 (7), 1150-1165 (2007)
   PUBMED   17513564
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   Lamping E, Monk BC, Niimi K, Holmes AR, Tsao S, Kaneko A, Niimi M,
            Uehara Y, Cannon RD.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2006) Oral Sciences, Univ. of Otago, 310 Gt. King
            St, Dunedin, Otago 9001, New Zealand
REFERENCE   3  (bases 1 to 6954)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6954)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic
            Cell"; date: "2007"; volume: "6"; issue: "7"; pages: "1150-1165"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-AUG-2006) Oral Sciences, Univ. of Otago, 310 Gt. King St,
            Dunedin, Otago 9001, New Zealand"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        623..641
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      687..1816
                     /label="Saccharomyces cerevisiae PDR5"
                     /note="Saccharomyces cerevisiae PDR5"
                     /regulatory_class="promoter"
     misc_feature    1817..1854
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     terminator      1919..2189
                     /label="PGK1 terminator"
                     /note="transcription terminator for 3-phosphoglycerate
                     kinase gene"
     CDS             3173..4063
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P00498"
     misc_feature    4445..4712
                     /label="Saccharomyces cerevisiae PDR5 gene, 3' end"
                     /note="Saccharomyces cerevisiae PDR5 gene, 3' end"
     promoter        complement(4766..4784)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4805..4821)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4829..4845)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4853..4883)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4898..4919)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5207..5795)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5969..6826)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6827..6931)
                     /label="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccgggcc
      661 ccccctgcag gtcaacggta tcgatggtcc gtcatatacg gttctccgtt gggcgcgccc
      721 acacacatat atataagcct gaatgcagta gacaaatttc agatatcata caaaggaagc
      781 gttctctggg acccctttct atgtttagat ggttcactga acttgttcgt taacgtaaat
      841 atgtcttcct ctttgattcc aacaatgata ttataaggaa attatcgtca caatctaatc
      901 aacaaggaca aagaaaagaa aaagttgaga gagagaacaa aagttcaaat caaagaaaaa
      961 aaagaacatc aaattaccta ttacaataaa caattaagcc atactcgcaa caatttgcca
     1021 taatagaaag cagcacctcg ttggcgcagt cccttacata gtacacaaca tttatcactt
     1081 cacacaatca ggagtggaac tcaatggaaa acaacaccac acgtatgatc ttactaataa
     1141 aagaacatga acgttcctca gcgcgaacgt tcgcattctg cgccttcgag cacaggataa
     1201 gttgcaggaa gccatcacat ctatgcaacg attatcacga cacaaccttg ccgccgagaa
     1261 aacgtccgtg gagaaccatt cggtcgattg cttcccacgg aacgagtgga ctgaaactta
     1321 agactgcccc tctctttccg cggaatcgct catgccgcgg tgccacaaca ttttcagatt
     1381 tactaagact ccggtgagtg tgggctcacc cgcgggtcgt gatcacgatt cagcaccctt
     1441 tggactcgtg attccgtgga aaggtcagat ctgtattcct acttatggta atgtgctaaa
     1501 aaaagagaaa tgtctccgcg gaactcttct acgccgtggt acgatatctg ttgaacgtaa
     1561 tctgagcaat acaaacaagg cctctcctat acatatataa ttgtgatgtg cataacctta
     1621 tggctgttcg cttttattac cataccttag aatgaaatcc aaaagaaaaa agtcacgcaa
     1681 agttgcaaac atataacaac tgtgttagtt atcactcgac tttgttattc taattataaa
     1741 taaattggca actaggaact ttcgaaaaag aaattaaaga cccttttaag ttttcgtatc
     1801 cgctcgttcg aaagacttaa ttaagctttc ccatgtctct actggtgcgg ccgcttcttt
     1861 ggaattattg gaaggtaagg aattgccagg tgttgctttc ttatccgaaa agaaataaat
     1921 tgaattgaat tgaaatcgat agatcaattt ttttcttttc tctttcccca tcctttacgc
     1981 taaaataata gtttatttta ttttttgaat attttttatt tatatacgta tatatagact
     2041 attatttatc ttttaatgat tattaagatt tttattaaaa aaaaattcgc tcctctttta
     2101 atgcctttat gcagtttttt tttcccattc gatatttcta tgttcgggtt cagcgtattt
     2161 taagtttaat aactcgaaaa ttctgcgttc gttggatcct tcagtgctta caatattgat
     2221 gattgggata attgggagca tctacctatc ggtatcgcag gtactgctgc cttaattgtt
     2281 ggtgccttcg gtgttgcatt gggtatgtgt caaacctatt gggttggtga aattggccgc
     2341 ttaattggta aatatggtgg tgacattggg ttcgagttgg gtgcaagttg ggccttcatc
     2401 atctacaaca tcttaagacc tttagaatta aaatacttcg gtcgttagga agtaatgatt
     2461 agggcgatca tttccccgtg cacatttcac gtcatgtatc atagatgcaa tttcatgtaa
     2521 actatttaga catctcattt aataaaaata gctacaaact gtattttaca atattagtaa
     2581 catatcaaag ataatctcta tggtatatac aaaacttcaa aaatgccaag acaatttgca
     2641 cggctgcgac ctaattgaga ctttgtggcc tgcatatcag tcttacaata aaagccaaaa
     2701 agaaaaaaaa aaagtaagaa tgcacaggtg actagtggtt atgtgactat gtgaacgtaa
     2761 agagaacgag atgtaacccg gcaacccggc tagagaggtt gtttagcatg cgaggtatta
     2821 gactccttgt caaaaaatta taaaatattt attagttaat ttattagttg caaagtcatt
     2881 gataacagcg tgactcttcc cggaaattct ttcatttcgg catttcgaag cgagaccgca
     2941 aaacacgtgt acgcacgtag ccaacgattg aaaaaaaaag cttccaagtg agtcacctct
     3001 accgttggaa cttataaaat atttttgcag aatatactcg cctgaaagtc catttatacg
     3061 cagtgaactg caagtattta ccgtctaaac tttggcattt tactcctatt aacggtttga
     3121 atctttgaaa atagaaaaga aaggataggt ttctaaaaaa ttcaatagaa aaatggattt
     3181 ggtgaaccat ctaaccgata gactactgtt tgcaatccca aagaaaggtc gtttatattc
     3241 taaaagtgtt tctattttga atggtgctga tattaccttt caccgctctc aaagattaga
     3301 cattgcacta agcacaagct tacctgtagc gttggtcttt ctgcccgctg cagatattcc
     3361 aacttttgtt ggtgaaggta aatgtgatct tggtataact ggtgttgacc aagttcgtga
     3421 atctaacgtc gacgtagact tagcaatcga tttgcaattt ggtaactgta aattgcaggt
     3481 acaagtcccc gtaaatggcg agtataaaaa gccagaacag ttaattggca aaaccattgt
     3541 taccagtttc gtgaaacttg ctgaaaaata ctttgccgat ttggaaggta ctactgttga
     3601 aaaaatgacc acaaggataa agtttgtcag tggttccgtg gaggcatcat gtgctctggg
     3661 aattggtgat gctattgtag atcttgtaga gagtggtgag acaatgaggg cagcaggttt
     3721 agttgatatt gccaccgtcc taagcacaag tgcctaccta atagaatcaa agaacccaaa
     3781 gagcgataag agtttgattg ctactatcaa atcaagaatt gaaggtgtca tgaccgctca
     3841 aaggttcgtt tcatgtattt ataacgcacc tgaagacaag ctgcctgaac tgttgaaggt
     3901 gacgcctggc cgtagagcac caaccatttc caaaattgac gatgaaggat gggttgctgt
     3961 tagttccatg attgagagaa aaacgaaggg tgttgtttta gatgaattga aaagactcgg
     4021 cgcatctgat atcatggttt tcgaaatttc taattgtcgt gtataagata gaacagaaaa
     4081 agggaagcca aatcccgatt aattttgtat tttttatagg caataatttg atagagttct
     4141 atcttattct aagttacccc tcatgacttc ccaagtgtgt tttgaggtta tctctgtgaa
     4201 tattttttct ttttttttgc tacttcccca ggaagttaaa tcttcgtagc atatttgtca
     4261 gctttactac cgtcttcatg ctcatgaaac cttcttcttc aacccccttc atttctccaa
     4321 ttttcgaaaa ctggagctac ccataaatat tcggagtttt gtaggtattt ttattttgga
     4381 gaaaacctgt acaaactctc cccagggagc atagattggg gatcgtaacg cataaagctg
     4441 tctagaattc acaccaccat ccggtatgac atgtgggcag tacatggaac catatttaca
     4501 actagcaaag actggttact taactgatga aaatgccact gacacctgta gtttctgtca
     4561 aatatctaca accaatgatt acttagctaa tgtcaattct ttctacagtg agagatggag
     4621 aaattatggt atcttcatct gttatattgc attcaattat atcgctggtg tctttttcta
     4681 ctggttagca agagtgccta aaaagaacgg taggcgcgcc aagtctagtg cggccggcca
     4741 agcggtggac ctcgagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
     4801 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     4861 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     4921 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4981 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     5041 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5101 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5161 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5221 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5281 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5341 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5401 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5461 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5521 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5581 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5641 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5701 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5761 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5821 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5881 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5941 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6001 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6061 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6121 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6181 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6241 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     6301 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6361 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6421 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6481 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6541 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     6601 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6661 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6721 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6781 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6841 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6901 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac
//
