LOCUS       V009628                 7641 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009628
VERSION     V009628
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Lamping E, Monk BC, Niimi K, Holmes AR, Tsao S, Tanabe K, Niimi M,
            Uehara Y, Cannon RD.
  TITLE     Characterization of three classes of membrane proteins involved in
            fungal azole resistance by functional hyperexpression in
            Saccharomyces cerevisiae
  JOURNAL   Eukaryotic Cell 6 (7), 1150-1165 (2007)
   PUBMED   17513564
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Lamping E, Monk BC, Niimi K.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2006) Oral Sciences, Univ. of Otago, 310 Gt. King
            St, Dunedin, Otago 9001, New Zealand
REFERENCE   3  (bases 1 to 7641)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7641)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic
            Cell"; date: "2007"; volume: "6"; issue: "7"; pages: "1150-1165"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-AUG-2006) Oral Sciences, Univ. of Otago, 310 Gt. King St,
            Dunedin, Otago 9001, New Zealand"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        623..641
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      687..1816
                     /label="Saccharomyces cerevisiae PDR5"
                     /note="Saccharomyces cerevisiae PDR5"
                     /regulatory_class="promoter"
     misc_feature    1817..1854
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     CDS             1855..2529
                     /label="mRFP1"
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     terminator      2606..2876
                     /label="PGK1 terminator"
                     /note="transcription terminator for 3-phosphoglycerate
                     kinase gene"
     CDS             3860..4750
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P00498"
     misc_feature    5132..5399
                     /label="Saccharomyces cerevisiae PDR5 gene, 3' end"
                     /note="Saccharomyces cerevisiae PDR5 gene, 3' end"
     promoter        complement(5453..5471)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5492..5508)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5516..5532)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5540..5570)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5585..5606)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5894..6482)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6656..7513)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7514..7618)
                     /label="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccgggcc
      661 ccccctgcag gtcaacggta tcgatggtcc gtcatatacg gttctccgtt gggcgcgccc
      721 acacacatat atataagcct gaatgcagta gacaaatttc agatatcata caaaggaagc
      781 gttctctggg acccctttct atgtttagat ggttcactga acttgttcgt taacgtaaat
      841 atgtcttcct ctttgattcc aacaatgata ttataaggaa attatcgtca caatctaatc
      901 aacaaggaca aagaaaagaa aaagttgaga gagagaacaa aagttcaaat caaagaaaaa
      961 aaagaacatc aaattaccta ttacaataaa caattaagcc atactcgcaa caatttgcca
     1021 taatagaaag cagcacctcg ttggcgcagt cccttacata gtacacaaca tttatcactt
     1081 cacacaatca ggagtggaac tcaatggaaa acaacaccac acgtatgatc ttactaataa
     1141 aagaacatga acgttcctca gcgcgaacgt tcgcattctg cgccttcgag cacaggataa
     1201 gttgcaggaa gccatcacat ctatgcaacg attatcacga cacaaccttg ccgccgagaa
     1261 aacgtccgtg gagaaccatt cggtcgattg cttcccacgg aacgagtgga ctgaaactta
     1321 agactgcccc tctctttccg cggaatcgct catgccgcgg tgccacaaca ttttcagatt
     1381 tactaagact ccggtgagtg tgggctcacc cgcgggtcgt gatcacgatt cagcaccctt
     1441 tggactcgtg attccgtgga aaggtcagat ctgtattcct acttatggta atgtgctaaa
     1501 aaaagagaaa tgtctccgcg gaactcttct acgccgtggt acgatatctg ttgaacgtaa
     1561 tctgagcaat acaaacaagg cctctcctat acatatataa ttgtgatgtg cataacctta
     1621 tggctgttcg cttttattac cataccttag aatgaaatcc aaaagaaaaa agtcacgcaa
     1681 agttgcaaac atataacaac tgtgttagtt atcactcgac tttgttattc taattataaa
     1741 taaattggca actaggaact ttcgaaaaag aaattaaaga cccttttaag ttttcgtatc
     1801 cgctcgttcg aaagacttaa ttaagctttc ccatgtctct actggtgcgg ccgcatggcc
     1861 tcctccgagg acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg
     1921 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     1981 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     2041 cctcagttcc agtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     2101 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     2161 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     2221 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     2281 tgggaggcct ccaccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     2341 atgaggctga agctgaagga cggcggccac tacgacgccg aggtcaagac cacctacatg
     2401 gccaagaagc ccgtgcagct gcccggcgcc tacaagaccg acatcaagct ggacatcacc
     2461 tcccacaacg aggactacac catcgtggaa cagtacgagc gcgccgaggg ccgccactcc
     2521 accggcgcct aaatcggccg cttctttgga attattggaa ggtaaggaat tgccaggtgt
     2581 tgctttctta tccgaaaaga aataaattga attgaattga aatcgataga tcaatttttt
     2641 tcttttctct ttccccatcc tttacgctaa aataatagtt tattttattt tttgaatatt
     2701 ttttatttat atacgtatat atagactatt atttatcttt taatgattat taagattttt
     2761 attaaaaaaa aattcgctcc tcttttaatg cctttatgca gttttttttt cccattcgat
     2821 atttctatgt tcgggttcag cgtattttaa gtttaataac tcgaaaattc tgcgttcgtt
     2881 ggatccttca gtgcttacaa tattgatgat tgggataatt gggagcatct acctatcggt
     2941 atcgcaggta ctgctgcctt aattgttggt gccttcggtg ttgcattggg tatgtgtcaa
     3001 acctattggg ttggtgaaat tggccgctta attggtaaat atggtggtga cattgggttc
     3061 gagttgggtg caagttgggc cttcatcatc tacaacatct taagaccttt agaattaaaa
     3121 tacttcggtc gttaggaagt aatgattagg gcgatcattt ccccgtgcac atttcacgtc
     3181 atgtatcata gatgcaattt catgtaaact atttagacat ctcatttaat aaaaatagct
     3241 acaaactgta ttttacaata ttagtaacat atcaaagata atctctatgg tatatacaaa
     3301 acttcaaaaa tgccaagaca atttgcacgg ctgcgaccta attgagactt tgtggcctgc
     3361 atatcagtct tacaataaaa gccaaaaaga aaaaaaaaaa gtaagaatgc acaggtgact
     3421 agtggttatg tgactatgtg aacgtaaaga gaacgagatg taacccggca acccggctag
     3481 agaggttgtt tagcatgcga ggtattagac tccttgtcaa aaaattataa aatatttatt
     3541 agttaattta ttagttgcaa agtcattgat aacagcgtga ctcttcccgg aaattctttc
     3601 atttcggcat ttcgaagcga gaccgcaaaa cacgtgtacg cacgtagcca acgattgaaa
     3661 aaaaaagctt ccaagtgagt cacctctacc gttggaactt ataaaatatt tttgcagaat
     3721 atactcgcct gaaagtccat ttatacgcag tgaactgcaa gtatttaccg tctaaacttt
     3781 ggcattttac tcctattaac ggtttgaatc tttgaaaata gaaaagaaag gataggtttc
     3841 taaaaaattc aatagaaaaa tggatttggt gaaccatcta accgatagac tactgtttgc
     3901 aatcccaaag aaaggtcgtt tatattctaa aagtgtttct attttgaatg gtgctgatat
     3961 tacctttcac cgctctcaaa gattagacat tgcactaagc acaagcttac ctgtagcgtt
     4021 ggtctttctg cccgctgcag atattccaac ttttgttggt gaaggtaaat gtgatcttgg
     4081 tataactggt gttgaccaag ttcgtgaatc taacgtcgac gtagacttag caatcgattt
     4141 gcaatttggt aactgtaaat tgcaggtaca agtccccgta aatggcgagt ataaaaagcc
     4201 agaacagtta attggcaaaa ccattgttac cagtttcgtg aaacttgctg aaaaatactt
     4261 tgccgatttg gaaggtacta ctgttgaaaa aatgaccaca aggataaagt ttgtcagtgg
     4321 ttccgtggag gcatcatgtg ctctgggaat tggtgatgct attgtagatc ttgtagagag
     4381 tggtgagaca atgagggcag caggtttagt tgatattgcc accgtcctaa gcacaagtgc
     4441 ctacctaata gaatcaaaga acccaaagag cgataagagt ttgattgcta ctatcaaatc
     4501 aagaattgaa ggtgtcatga ccgctcaaag gttcgtttca tgtatttata acgcacctga
     4561 agacaagctg cctgaactgt tgaaggtgac gcctggccgt agagcaccaa ccatttccaa
     4621 aattgacgat gaaggatggg ttgctgttag ttccatgatt gagagaaaaa cgaagggtgt
     4681 tgttttagat gaattgaaaa gactcggcgc atctgatatc atggttttcg aaatttctaa
     4741 ttgtcgtgta taagatagaa cagaaaaagg gaagccaaat cccgattaat tttgtatttt
     4801 ttataggcaa taatttgata gagttctatc ttattctaag ttacccctca tgacttccca
     4861 agtgtgtttt gaggttatct ctgtgaatat tttttctttt tttttgctac ttccccagga
     4921 agttaaatct tcgtagcata tttgtcagct ttactaccgt cttcatgctc atgaaacctt
     4981 cttcttcaac ccccttcatt tctccaattt tcgaaaactg gagctaccca taaatattcg
     5041 gagttttgta ggtattttta ttttggagaa aacctgtaca aactctcccc agggagcata
     5101 gattggggat cgtaacgcat aaagctgtct agaattcaca ccaccatccg gtatgacatg
     5161 tgggcagtac atggaaccat atttacaact agcaaagact ggttacttaa ctgatgaaaa
     5221 tgccactgac acctgtagtt tctgtcaaat atctacaacc aatgattact tagctaatgt
     5281 caattctttc tacagtgaga gatggagaaa ttatggtatc ttcatctgtt atattgcatt
     5341 caattatatc gctggtgtct ttttctactg gttagcaaga gtgcctaaaa agaacggtag
     5401 gcgcgccaag tctagtgcgg ccggccaagc ggtggacctc gagcttttgt tccctttagt
     5461 gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5521 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5581 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5641 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5701 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5761 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5821 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5881 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5941 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6001 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6061 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6121 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6181 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6241 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6301 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     6361 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6421 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6481 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6541 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6601 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6661 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6721 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6781 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6841 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     6901 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     6961 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7021 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7081 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7141 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7201 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7261 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7321 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7381 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7441 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7501 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7561 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7621 catttccccg aaaagtgcca c
//
