LOCUS       V009629                 7003 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009629
VERSION     V009629
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7003)
  AUTHORS   Lamping E, Monk BC, Niimi K, Holmes AR, Tsao S, Tanabe K, Niimi M,
            Uehara Y, Cannon RD.
  TITLE     Characterization of three classes of membrane proteins involved in
            fungal azole resistance by functional hyperexpression in
            Saccharomyces cerevisiae
  JOURNAL   Eukaryotic Cell 6 (7), 1150-1165 (2007)
   PUBMED   17513564
REFERENCE   2  (bases 1 to 7003)
  AUTHORS   Lamping E, Monk BC, Niimi K, Holmes AR, Tsao S, Kaneko A, Niimi M,
            Uehara Y, Cannon RD.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2006) Oral Sciences, Univ. of Otago, 310 Gt. King
            St, Dunedin, Otago 9001, New Zealand
REFERENCE   3  (bases 1 to 7003)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7003)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic
            Cell"; date: "2007"; volume: "6"; issue: "7"; pages: "1150-1165"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-AUG-2006) Oral Sciences, Univ. of Otago, 310 Gt. King St,
            Dunedin, Otago 9001, New Zealand"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        623..641
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      687..1816
                     /label="Saccharomyces cerevisiae PDR5"
                     /note="Saccharomyces cerevisiae PDR5"
                     /regulatory_class="promoter"
     misc_feature    1817..1854
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     CDS             1855..1872
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             1873..1890
                     /label="tetracysteine tag"
                     /note="tetracysteine peptide that binds biarsenical
                     labeling reagents"
     terminator      1968..2238
                     /label="PGK1 terminator"
                     /note="transcription terminator for 3-phosphoglycerate
                     kinase gene"
     CDS             3222..4112
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P00498"
     misc_feature    4494..4761
                     /label="Saccharomyces cerevisiae PDR5 gene, 3' end"
                     /note="Saccharomyces cerevisiae PDR5 gene, 3' end"
     promoter        complement(4815..4833)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4854..4870)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4878..4894)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4902..4932)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4947..4968)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5256..5844)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6018..6875)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6876..6980)
                     /label="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccgggcc
      661 ccccctgcag gtcaacggta tcgatggtcc gtcatatacg gttctccgtt gggcgcgccc
      721 acacacatat atataagcct gaatgcagta gacaaatttc agatatcata caaaggaagc
      781 gttctctggg acccctttct atgtttagat ggttcactga acttgttcgt taacgtaaat
      841 atgtcttcct ctttgattcc aacaatgata ttataaggaa attatcgtca caatctaatc
      901 aacaaggaca aagaaaagaa aaagttgaga gagagaacaa aagttcaaat caaagaaaaa
      961 aaagaacatc aaattaccta ttacaataaa caattaagcc atactcgcaa caatttgcca
     1021 taatagaaag cagcacctcg ttggcgcagt cccttacata gtacacaaca tttatcactt
     1081 cacacaatca ggagtggaac tcaatggaaa acaacaccac acgtatgatc ttactaataa
     1141 aagaacatga acgttcctca gcgcgaacgt tcgcattctg cgccttcgag cacaggataa
     1201 gttgcaggaa gccatcacat ctatgcaacg attatcacga cacaaccttg ccgccgagaa
     1261 aacgtccgtg gagaaccatt cggtcgattg cttcccacgg aacgagtgga ctgaaactta
     1321 agactgcccc tctctttccg cggaatcgct catgccgcgg tgccacaaca ttttcagatt
     1381 tactaagact ccggtgagtg tgggctcacc cgcgggtcgt gatcacgatt cagcaccctt
     1441 tggactcgtg attccgtgga aaggtcagat ctgtattcct acttatggta atgtgctaaa
     1501 aaaagagaaa tgtctccgcg gaactcttct acgccgtggt acgatatctg ttgaacgtaa
     1561 tctgagcaat acaaacaagg cctctcctat acatatataa ttgtgatgtg cataacctta
     1621 tggctgttcg cttttattac cataccttag aatgaaatcc aaaagaaaaa agtcacgcaa
     1681 agttgcaaac atataacaac tgtgttagtt atcactcgac tttgttattc taattataaa
     1741 taaattggca actaggaact ttcgaaaaag aaattaaaga cccttttaag ttttcgtatc
     1801 cgctcgttcg aaagacttaa ttaagctttc ccatgtctct actggtgcgg ccgccatcat
     1861 caccatcatc attgttgtcc aggttgctgt taaattcggc cgcttctttg gaattattgg
     1921 aaggtaagga attgccaggt gttgctttct tatccgaaaa gaaataaatt gaattgaatt
     1981 gaaatcgata gatcaatttt tttcttttct ctttccccat cctttacgct aaaataatag
     2041 tttattttat tttttgaata ttttttattt atatacgtat atatagacta ttatttatct
     2101 tttaatgatt attaagattt ttattaaaaa aaaattcgct cctcttttaa tgcctttatg
     2161 cagttttttt ttcccattcg atatttctat gttcgggttc agcgtatttt aagtttaata
     2221 actcgaaaat tctgcgttcg ttggatcctt cagtgcttac aatattgatg attgggataa
     2281 ttgggagcat ctacctatcg gtatcgcagg tactgctgcc ttaattgttg gtgccttcgg
     2341 tgttgcattg ggtatgtgtc aaacctattg ggttggtgaa attggccgct taattggtaa
     2401 atatggtggt gacattgggt tcgagttggg tgcaagttgg gccttcatca tctacaacat
     2461 cttaagacct ttagaattaa aatacttcgg tcgttaggaa gtaatgatta gggcgatcat
     2521 ttccccgtgc acatttcacg tcatgtatca tagatgcaat ttcatgtaaa ctatttagac
     2581 atctcattta ataaaaatag ctacaaactg tattttacaa tattagtaac atatcaaaga
     2641 taatctctat ggtatataca aaacttcaaa aatgccaaga caatttgcac ggctgcgacc
     2701 taattgagac tttgtggcct gcatatcagt cttacaataa aagccaaaaa gaaaaaaaaa
     2761 aagtaagaat gcacaggtga ctagtggtta tgtgactatg tgaacgtaaa gagaacgaga
     2821 tgtaacccgg caacccggct agagaggttg tttagcatgc gaggtattag actccttgtc
     2881 aaaaaattat aaaatattta ttagttaatt tattagttgc aaagtcattg ataacagcgt
     2941 gactcttccc ggaaattctt tcatttcggc atttcgaagc gagaccgcaa aacacgtgta
     3001 cgcacgtagc caacgattga aaaaaaaagc ttccaagtga gtcacctcta ccgttggaac
     3061 ttataaaata tttttgcaga atatactcgc ctgaaagtcc atttatacgc agtgaactgc
     3121 aagtatttac cgtctaaact ttggcatttt actcctatta acggtttgaa tctttgaaaa
     3181 tagaaaagaa aggataggtt tctaaaaaat tcaatagaaa aatggatttg gtgaaccatc
     3241 taaccgatag actactgttt gcaatcccaa agaaaggtcg tttatattct aaaagtgttt
     3301 ctattttgaa tggtgctgat attacctttc accgctctca aagattagac attgcactaa
     3361 gcacaagctt acctgtagcg ttggtctttc tgcccgctgc agatattcca acttttgttg
     3421 gtgaaggtaa atgtgatctt ggtataactg gtgttgacca agttcgtgaa tctaacgtcg
     3481 acgtagactt agcaatcgat ttgcaatttg gtaactgtaa attgcaggta caagtccccg
     3541 taaatggcga gtataaaaag ccagaacagt taattggcaa aaccattgtt accagtttcg
     3601 tgaaacttgc tgaaaaatac tttgccgatt tggaaggtac tactgttgaa aaaatgacca
     3661 caaggataaa gtttgtcagt ggttccgtgg aggcatcatg tgctctggga attggtgatg
     3721 ctattgtaga tcttgtagag agtggtgaga caatgagggc agcaggttta gttgatattg
     3781 ccaccgtcct aagcacaagt gcctacctaa tagaatcaaa gaacccaaag agcgataaga
     3841 gtttgattgc tactatcaaa tcaagaattg aaggtgtcat gaccgctcaa aggttcgttt
     3901 catgtattta taacgcacct gaagacaagc tgcctgaact gttgaaggtg acgcctggcc
     3961 gtagagcacc aaccatttcc aaaattgacg atgaaggatg ggttgctgtt agttccatga
     4021 ttgagagaaa aacgaagggt gttgttttag atgaattgaa aagactcggc gcatctgata
     4081 tcatggtttt cgaaatttct aattgtcgtg tataagatag aacagaaaaa gggaagccaa
     4141 atcccgatta attttgtatt ttttataggc aataatttga tagagttcta tcttattcta
     4201 agttacccct catgacttcc caagtgtgtt ttgaggttat ctctgtgaat attttttctt
     4261 tttttttgct acttccccag gaagttaaat cttcgtagca tatttgtcag ctttactacc
     4321 gtcttcatgc tcatgaaacc ttcttcttca acccccttca tttctccaat tttcgaaaac
     4381 tggagctacc cataaatatt cggagttttg taggtatttt tattttggag aaaacctgta
     4441 caaactctcc ccagggagca tagattgggg atcgtaacgc ataaagctgt ctagaattca
     4501 caccaccatc cggtatgaca tgtgggcagt acatggaacc atatttacaa ctagcaaaga
     4561 ctggttactt aactgatgaa aatgccactg acacctgtag tttctgtcaa atatctacaa
     4621 ccaatgatta cttagctaat gtcaattctt tctacagtga gagatggaga aattatggta
     4681 tcttcatctg ttatattgca ttcaattata tcgctggtgt ctttttctac tggttagcaa
     4741 gagtgcctaa aaagaacggt aggcgcgcca agtctagtgc ggccggccaa gcggtggacc
     4801 tcgagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag
     4861 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     4921 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     4981 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     5041 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     5101 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5161 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5221 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5281 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5341 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5401 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5461 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5521 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5581 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5641 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     5701 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5761 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5821 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5881 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5941 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6001 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6061 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6121 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6181 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6241 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6301 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     6361 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     6421 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     6481 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     6541 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     6601 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     6661 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     6721 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     6781 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     6841 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     6901 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     6961 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//
