LOCUS       V009630                 6985 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009630
VERSION     V009630
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Lamping E, Monk BC, Niimi K, Holmes AR, Tsao S, Tanabe K, Niimi M,
            Uehara Y, Cannon RD.
  TITLE     Characterization of three classes of membrane proteins involved in
            fungal azole resistance by functional hyperexpression in
            Saccharomyces cerevisiae
  JOURNAL   Eukaryotic Cell 6 (7), 1150-1165 (2007)
   PUBMED   17513564
REFERENCE   2  (bases 1 to 6985)
  AUTHORS   Lamping E, Monk BC, Niimi K, Holmes AR, Tsao S, Kaneko A, Niimi M,
            Uehara Y, Cannon RD.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2006) Oral Sciences, Univ. of Otago, 310 Gt. King
            St, Dunedin, Otago 9001, New Zealand
REFERENCE   3  (bases 1 to 6985)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6985)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic
            Cell"; date: "2007"; volume: "6"; issue: "7"; pages: "1150-1165"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-AUG-2006) Oral Sciences, Univ. of Otago, 310 Gt. King St,
            Dunedin, Otago 9001, New Zealand"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        623..641
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      687..1816
                     /label="Saccharomyces cerevisiae PDR5"
                     /note="Saccharomyces cerevisiae PDR5"
                     /regulatory_class="promoter"
     misc_feature    1817..1854
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     CDS             1855..1872
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      1950..2220
                     /label="PGK1 terminator"
                     /note="transcription terminator for 3-phosphoglycerate
                     kinase gene"
     CDS             3204..4094
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P00498"
     misc_feature    4476..4743
                     /label="Saccharomyces cerevisiae PDR5 gene, 3' end"
                     /note="Saccharomyces cerevisiae PDR5 gene, 3' end"
     promoter        complement(4797..4815)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4836..4852)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4860..4876)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4884..4914)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4929..4950)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5238..5826)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6000..6857)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6858..6962)
                     /label="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccgggcc
      661 ccccctgcag gtcaacggta tcgatggtcc gtcatatacg gttctccgtt gggcgcgccc
      721 acacacatat atataagcct gaatgcagta gacaaatttc agatatcata caaaggaagc
      781 gttctctggg acccctttct atgtttagat ggttcactga acttgttcgt taacgtaaat
      841 atgtcttcct ctttgattcc aacaatgata ttataaggaa attatcgtca caatctaatc
      901 aacaaggaca aagaaaagaa aaagttgaga gagagaacaa aagttcaaat caaagaaaaa
      961 aaagaacatc aaattaccta ttacaataaa caattaagcc atactcgcaa caatttgcca
     1021 taatagaaag cagcacctcg ttggcgcagt cccttacata gtacacaaca tttatcactt
     1081 cacacaatca ggagtggaac tcaatggaaa acaacaccac acgtatgatc ttactaataa
     1141 aagaacatga acgttcctca gcgcgaacgt tcgcattctg cgccttcgag cacaggataa
     1201 gttgcaggaa gccatcacat ctatgcaacg attatcacga cacaaccttg ccgccgagaa
     1261 aacgtccgtg gagaaccatt cggtcgattg cttcccacgg aacgagtgga ctgaaactta
     1321 agactgcccc tctctttccg cggaatcgct catgccgcgg tgccacaaca ttttcagatt
     1381 tactaagact ccggtgagtg tgggctcacc cgcgggtcgt gatcacgatt cagcaccctt
     1441 tggactcgtg attccgtgga aaggtcagat ctgtattcct acttatggta atgtgctaaa
     1501 aaaagagaaa tgtctccgcg gaactcttct acgccgtggt acgatatctg ttgaacgtaa
     1561 tctgagcaat acaaacaagg cctctcctat acatatataa ttgtgatgtg cataacctta
     1621 tggctgttcg cttttattac cataccttag aatgaaatcc aaaagaaaaa agtcacgcaa
     1681 agttgcaaac atataacaac tgtgttagtt atcactcgac tttgttattc taattataaa
     1741 taaattggca actaggaact ttcgaaaaag aaattaaaga cccttttaag ttttcgtatc
     1801 cgctcgttcg aaagacttaa ttaagctttc ccatgtctct actggtgcgg ccgccatcat
     1861 caccatcatc attaaattcg gccgcttctt tggaattatt ggaaggtaag gaattgccag
     1921 gtgttgcttt cttatccgaa aagaaataaa ttgaattgaa ttgaaatcga tagatcaatt
     1981 tttttctttt ctctttcccc atcctttacg ctaaaataat agtttatttt attttttgaa
     2041 tattttttat ttatatacgt atatatagac tattatttat cttttaatga ttattaagat
     2101 ttttattaaa aaaaaattcg ctcctctttt aatgccttta tgcagttttt ttttcccatt
     2161 cgatatttct atgttcgggt tcagcgtatt ttaagtttaa taactcgaaa attctgcgtt
     2221 cgttggatcc ttcagtgctt acaatattga tgattgggat aattgggagc atctacctat
     2281 cggtatcgca ggtactgctg ccttaattgt tggtgccttc ggtgttgcat tgggtatgtg
     2341 tcaaacctat tgggttggtg aaattggccg cttaattggt aaatatggtg gtgacattgg
     2401 gttcgagttg ggtgcaagtt gggccttcat catctacaac atcttaagac ctttagaatt
     2461 aaaatacttc ggtcgttagg aagtaatgat tagggcgatc atttccccgt gcacatttca
     2521 cgtcatgtat catagatgca atttcatgta aactatttag acatctcatt taataaaaat
     2581 agctacaaac tgtattttac aatattagta acatatcaaa gataatctct atggtatata
     2641 caaaacttca aaaatgccaa gacaatttgc acggctgcga cctaattgag actttgtggc
     2701 ctgcatatca gtcttacaat aaaagccaaa aagaaaaaaa aaaagtaaga atgcacaggt
     2761 gactagtggt tatgtgacta tgtgaacgta aagagaacga gatgtaaccc ggcaacccgg
     2821 ctagagaggt tgtttagcat gcgaggtatt agactccttg tcaaaaaatt ataaaatatt
     2881 tattagttaa tttattagtt gcaaagtcat tgataacagc gtgactcttc ccggaaattc
     2941 tttcatttcg gcatttcgaa gcgagaccgc aaaacacgtg tacgcacgta gccaacgatt
     3001 gaaaaaaaaa gcttccaagt gagtcacctc taccgttgga acttataaaa tatttttgca
     3061 gaatatactc gcctgaaagt ccatttatac gcagtgaact gcaagtattt accgtctaaa
     3121 ctttggcatt ttactcctat taacggtttg aatctttgaa aatagaaaag aaaggatagg
     3181 tttctaaaaa attcaataga aaaatggatt tggtgaacca tctaaccgat agactactgt
     3241 ttgcaatccc aaagaaaggt cgtttatatt ctaaaagtgt ttctattttg aatggtgctg
     3301 atattacctt tcaccgctct caaagattag acattgcact aagcacaagc ttacctgtag
     3361 cgttggtctt tctgcccgct gcagatattc caacttttgt tggtgaaggt aaatgtgatc
     3421 ttggtataac tggtgttgac caagttcgtg aatctaacgt cgacgtagac ttagcaatcg
     3481 atttgcaatt tggtaactgt aaattgcagg tacaagtccc cgtaaatggc gagtataaaa
     3541 agccagaaca gttaattggc aaaaccattg ttaccagttt cgtgaaactt gctgaaaaat
     3601 actttgccga tttggaaggt actactgttg aaaaaatgac cacaaggata aagtttgtca
     3661 gtggttccgt ggaggcatca tgtgctctgg gaattggtga tgctattgta gatcttgtag
     3721 agagtggtga gacaatgagg gcagcaggtt tagttgatat tgccaccgtc ctaagcacaa
     3781 gtgcctacct aatagaatca aagaacccaa agagcgataa gagtttgatt gctactatca
     3841 aatcaagaat tgaaggtgtc atgaccgctc aaaggttcgt ttcatgtatt tataacgcac
     3901 ctgaagacaa gctgcctgaa ctgttgaagg tgacgcctgg ccgtagagca ccaaccattt
     3961 ccaaaattga cgatgaagga tgggttgctg ttagttccat gattgagaga aaaacgaagg
     4021 gtgttgtttt agatgaattg aaaagactcg gcgcatctga tatcatggtt ttcgaaattt
     4081 ctaattgtcg tgtataagat agaacagaaa aagggaagcc aaatcccgat taattttgta
     4141 ttttttatag gcaataattt gatagagttc tatcttattc taagttaccc ctcatgactt
     4201 cccaagtgtg ttttgaggtt atctctgtga atattttttc tttttttttg ctacttcccc
     4261 aggaagttaa atcttcgtag catatttgtc agctttacta ccgtcttcat gctcatgaaa
     4321 ccttcttctt caaccccctt catttctcca attttcgaaa actggagcta cccataaata
     4381 ttcggagttt tgtaggtatt tttattttgg agaaaacctg tacaaactct ccccagggag
     4441 catagattgg ggatcgtaac gcataaagct gtctagaatt cacaccacca tccggtatga
     4501 catgtgggca gtacatggaa ccatatttac aactagcaaa gactggttac ttaactgatg
     4561 aaaatgccac tgacacctgt agtttctgtc aaatatctac aaccaatgat tacttagcta
     4621 atgtcaattc tttctacagt gagagatgga gaaattatgg tatcttcatc tgttatattg
     4681 cattcaatta tatcgctggt gtctttttct actggttagc aagagtgcct aaaaagaacg
     4741 gtaggcgcgc caagtctagt gcggccggcc aagcggtgga cctcgagctt ttgttccctt
     4801 tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     4861 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     4921 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     4981 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     5041 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5101 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5161 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5221 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5281 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5341 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5401 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5461 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5521 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5581 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5641 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     5701 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5761 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5821 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5881 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     5941 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     6001 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     6061 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     6121 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     6181 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     6241 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     6301 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     6361 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     6421 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     6481 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6541 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     6601 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     6661 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     6721 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     6781 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     6841 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     6901 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6961 cgcacatttc cccgaaaagt gccac
//
