LOCUS       V009631                 7680 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009631
VERSION     V009631
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Lamping E, Monk BC, Niimi K, Holmes AR, Tsao S, Tanabe K, Niimi M,
            Uehara Y, Cannon RD.
  TITLE     Characterization of three classes of membrane proteins involved in
            fungal azole resistance by functional hyperexpression in
            Saccharomyces cerevisiae
  JOURNAL   Eukaryotic Cell 6 (7), 1150-1165 (2007)
   PUBMED   17513564
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   Lamping E, Monk BC, Niimi K, Holmes AR, Tsao S, Kaneko A, Niimi M,
            Uehara Y, Cannon RD.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2006) Oral Sciences, Univ. of Otago, 310 Gt. King
            St, Dunedin, Otago 9001, New Zealand
REFERENCE   3  (bases 1 to 7680)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7680)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic
            Cell"; date: "2007"; volume: "6"; issue: "7"; pages: "1150-1165"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-AUG-2006) Oral Sciences, Univ. of Otago, 310 Gt. King St,
            Dunedin, Otago 9001, New Zealand"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        623..641
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      687..1816
                     /label="Saccharomyces cerevisiae PDR5"
                     /note="Saccharomyces cerevisiae PDR5"
                     /regulatory_class="promoter"
     misc_feature    1817..1854
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     CDS             1855..2568
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     terminator      2645..2915
                     /label="PGK1 terminator"
                     /note="transcription terminator for 3-phosphoglycerate
                     kinase gene"
     CDS             3899..4789
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P00498"
     misc_feature    5171..5438
                     /label="Saccharomyces cerevisiae PDR5 gene, 3' end"
                     /note="Saccharomyces cerevisiae PDR5 gene, 3' end"
     promoter        complement(5492..5510)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5531..5547)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5555..5571)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5579..5609)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5624..5645)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5933..6521)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6695..7552)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7553..7657)
                     /label="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccgggcc
      661 ccccctgcag gtcaacggta tcgatggtcc gtcatatacg gttctccgtt gggcgcgccc
      721 acacacatat atataagcct gaatgcagta gacaaatttc agatatcata caaaggaagc
      781 gttctctggg acccctttct atgtttagat ggttcactga acttgttcgt taacgtaaat
      841 atgtcttcct ctttgattcc aacaatgata ttataaggaa attatcgtca caatctaatc
      901 aacaaggaca aagaaaagaa aaagttgaga gagagaacaa aagttcaaat caaagaaaaa
      961 aaagaacatc aaattaccta ttacaataaa caattaagcc atactcgcaa caatttgcca
     1021 taatagaaag cagcacctcg ttggcgcagt cccttacata gtacacaaca tttatcactt
     1081 cacacaatca ggagtggaac tcaatggaaa acaacaccac acgtatgatc ttactaataa
     1141 aagaacatga acgttcctca gcgcgaacgt tcgcattctg cgccttcgag cacaggataa
     1201 gttgcaggaa gccatcacat ctatgcaacg attatcacga cacaaccttg ccgccgagaa
     1261 aacgtccgtg gagaaccatt cggtcgattg cttcccacgg aacgagtgga ctgaaactta
     1321 agactgcccc tctctttccg cggaatcgct catgccgcgg tgccacaaca ttttcagatt
     1381 tactaagact ccggtgagtg tgggctcacc cgcgggtcgt gatcacgatt cagcaccctt
     1441 tggactcgtg attccgtgga aaggtcagat ctgtattcct acttatggta atgtgctaaa
     1501 aaaagagaaa tgtctccgcg gaactcttct acgccgtggt acgatatctg ttgaacgtaa
     1561 tctgagcaat acaaacaagg cctctcctat acatatataa ttgtgatgtg cataacctta
     1621 tggctgttcg cttttattac cataccttag aatgaaatcc aaaagaaaaa agtcacgcaa
     1681 agttgcaaac atataacaac tgtgttagtt atcactcgac tttgttattc taattataaa
     1741 taaattggca actaggaact ttcgaaaaag aaattaaaga cccttttaag ttttcgtatc
     1801 cgctcgttcg aaagacttaa ttaagctttc ccatgtctct actggtgcgg ccgcatgtct
     1861 aaaggtgaag aattattcac tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt
     1921 aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg atgctactta cggtaaattg
     1981 accttaaaat ttatttgtac tactggtaaa ttgccagttc catggccaac cttagtcact
     2041 actttcggtt atggtgttca atgttttgcg agatacccag atcatatgaa acaacatgac
     2101 tttttcaagt ctgccatgcc agaaggttat gttcaagaaa gaactatttt tttcaaagat
     2161 gacggtaact acaagaccag agctgaagtc aagtttgaag gtgatacctt agttaataga
     2221 atcgaattaa aaggtattga ttttaaagaa gatggtaaca ttttaggtca caaattggaa
     2281 tacaactata actctcacaa tgtttacatc atggctgaca aacaaaagaa tggtatcaaa
     2341 gttaacttca aaattagaca caacattgaa gatggttctg ttcaattagc tgaccattat
     2401 caacaaaata ctccaattgg tgatggtcca gtcttgttac cagacaacca ttacttatcc
     2461 actcaatctg ccttatccaa agatccaaac gaaaagagag accacatggt cttgttagaa
     2521 tttgttactg ctgctggtat tacccatggt atggatgaat tgtacaaata aatcggccgc
     2581 ttctttggaa ttattggaag gtaaggaatt gccaggtgtt gctttcttat ccgaaaagaa
     2641 ataaattgaa ttgaattgaa atcgatagat caattttttt cttttctctt tccccatcct
     2701 ttacgctaaa ataatagttt attttatttt ttgaatattt tttatttata tacgtatata
     2761 tagactatta tttatctttt aatgattatt aagattttta ttaaaaaaaa attcgctcct
     2821 cttttaatgc ctttatgcag tttttttttc ccattcgata tttctatgtt cgggttcagc
     2881 gtattttaag tttaataact cgaaaattct gcgttcgttg gatccttcag tgcttacaat
     2941 attgatgatt gggataattg ggagcatcta cctatcggta tcgcaggtac tgctgcctta
     3001 attgttggtg ccttcggtgt tgcattgggt atgtgtcaaa cctattgggt tggtgaaatt
     3061 ggccgcttaa ttggtaaata tggtggtgac attgggttcg agttgggtgc aagttgggcc
     3121 ttcatcatct acaacatctt aagaccttta gaattaaaat acttcggtcg ttaggaagta
     3181 atgattaggg cgatcatttc cccgtgcaca tttcacgtca tgtatcatag atgcaatttc
     3241 atgtaaacta tttagacatc tcatttaata aaaatagcta caaactgtat tttacaatat
     3301 tagtaacata tcaaagataa tctctatggt atatacaaaa cttcaaaaat gccaagacaa
     3361 tttgcacggc tgcgacctaa ttgagacttt gtggcctgca tatcagtctt acaataaaag
     3421 ccaaaaagaa aaaaaaaaag taagaatgca caggtgacta gtggttatgt gactatgtga
     3481 acgtaaagag aacgagatgt aacccggcaa cccggctaga gaggttgttt agcatgcgag
     3541 gtattagact ccttgtcaaa aaattataaa atatttatta gttaatttat tagttgcaaa
     3601 gtcattgata acagcgtgac tcttcccgga aattctttca tttcggcatt tcgaagcgag
     3661 accgcaaaac acgtgtacgc acgtagccaa cgattgaaaa aaaaagcttc caagtgagtc
     3721 acctctaccg ttggaactta taaaatattt ttgcagaata tactcgcctg aaagtccatt
     3781 tatacgcagt gaactgcaag tatttaccgt ctaaactttg gcattttact cctattaacg
     3841 gtttgaatct ttgaaaatag aaaagaaagg ataggtttct aaaaaattca atagaaaaat
     3901 ggatttggtg aaccatctaa ccgatagact actgtttgca atcccaaaga aaggtcgttt
     3961 atattctaaa agtgtttcta ttttgaatgg tgctgatatt acctttcacc gctctcaaag
     4021 attagacatt gcactaagca caagcttacc tgtagcgttg gtctttctgc ccgctgcaga
     4081 tattccaact tttgttggtg aaggtaaatg tgatcttggt ataactggtg ttgaccaagt
     4141 tcgtgaatct aacgtcgacg tagacttagc aatcgatttg caatttggta actgtaaatt
     4201 gcaggtacaa gtccccgtaa atggcgagta taaaaagcca gaacagttaa ttggcaaaac
     4261 cattgttacc agtttcgtga aacttgctga aaaatacttt gccgatttgg aaggtactac
     4321 tgttgaaaaa atgaccacaa ggataaagtt tgtcagtggt tccgtggagg catcatgtgc
     4381 tctgggaatt ggtgatgcta ttgtagatct tgtagagagt ggtgagacaa tgagggcagc
     4441 aggtttagtt gatattgcca ccgtcctaag cacaagtgcc tacctaatag aatcaaagaa
     4501 cccaaagagc gataagagtt tgattgctac tatcaaatca agaattgaag gtgtcatgac
     4561 cgctcaaagg ttcgtttcat gtatttataa cgcacctgaa gacaagctgc ctgaactgtt
     4621 gaaggtgacg cctggccgta gagcaccaac catttccaaa attgacgatg aaggatgggt
     4681 tgctgttagt tccatgattg agagaaaaac gaagggtgtt gttttagatg aattgaaaag
     4741 actcggcgca tctgatatca tggttttcga aatttctaat tgtcgtgtat aagatagaac
     4801 agaaaaaggg aagccaaatc ccgattaatt ttgtattttt tataggcaat aatttgatag
     4861 agttctatct tattctaagt tacccctcat gacttcccaa gtgtgttttg aggttatctc
     4921 tgtgaatatt ttttcttttt ttttgctact tccccaggaa gttaaatctt cgtagcatat
     4981 ttgtcagctt tactaccgtc ttcatgctca tgaaaccttc ttcttcaacc cccttcattt
     5041 ctccaatttt cgaaaactgg agctacccat aaatattcgg agttttgtag gtatttttat
     5101 tttggagaaa acctgtacaa actctcccca gggagcatag attggggatc gtaacgcata
     5161 aagctgtcta gaattcacac caccatccgg tatgacatgt gggcagtaca tggaaccata
     5221 tttacaacta gcaaagactg gttacttaac tgatgaaaat gccactgaca cctgtagttt
     5281 ctgtcaaata tctacaacca atgattactt agctaatgtc aattctttct acagtgagag
     5341 atggagaaat tatggtatct tcatctgtta tattgcattc aattatatcg ctggtgtctt
     5401 tttctactgg ttagcaagag tgcctaaaaa gaacggtagg cgcgccaagt ctagtgcggc
     5461 cggccaagcg gtggacctcg agcttttgtt ccctttagtg agggttaatt tcgagcttgg
     5521 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     5581 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     5641 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     5701 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     5761 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     5821 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     5881 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5941 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6001 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6061 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6121 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6181 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6241 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     6301 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6361 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6421 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     6481 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6541 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     6601 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     6661 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6721 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6781 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6841 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6901 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6961 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     7021 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     7081 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     7141 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     7201 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     7261 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     7321 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     7381 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     7441 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     7501 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     7561 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     7621 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//
