LOCUS       V009632                 7009 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009632
VERSION     V009632
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7009)
  AUTHORS   Lamping E, Monk BC, Niimi K, Holmes AR, Tsao S, Tanabe K, Niimi M,
            Uehara Y, Cannon RD.
  TITLE     Characterization of three classes of membrane proteins involved in
            fungal azole resistance by functional hyperexpression in
            Saccharomyces cerevisiae
  JOURNAL   Eukaryotic Cell 6 (7), 1150-1165 (2007)
   PUBMED   17513564
REFERENCE   2  (bases 1 to 7009)
  AUTHORS   Lamping E, Monk BC, Niimi K, Holmes AR, Tsao S, Kaneko A, Niimi M,
            Uehara Y, Cannon RD.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2006) Oral Sciences, Univ. of Otago, 310 Gt. King
            St, Dunedin, Otago 9001, New Zealand
REFERENCE   3  (bases 1 to 7009)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7009)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic
            Cell"; date: "2007"; volume: "6"; issue: "7"; pages: "1150-1165"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-AUG-2006) Oral Sciences, Univ. of Otago, 310 Gt. King St,
            Dunedin, Otago 9001, New Zealand"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        623..641
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      687..1816
                     /label="Saccharomyces cerevisiae PDR5"
                     /note="Saccharomyces cerevisiae PDR5"
                     /regulatory_class="promoter"
     misc_feature    1817..1854
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     CDS             1855..1878
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             1879..1896
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      1974..2244
                     /label="PGK1 terminator"
                     /note="transcription terminator for 3-phosphoglycerate
                     kinase gene"
     CDS             3228..4118
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P00498"
     misc_feature    4500..4767
                     /label="Saccharomyces cerevisiae PDR5 gene, 3' end"
                     /note="Saccharomyces cerevisiae PDR5 gene, 3' end"
     promoter        complement(4821..4839)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4860..4876)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4884..4900)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4908..4938)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4953..4974)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5262..5850)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6024..6881)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6882..6986)
                     /label="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccgggcc
      661 ccccctgcag gtcaacggta tcgatggtcc gtcatatacg gttctccgtt gggcgcgccc
      721 acacacatat atataagcct gaatgcagta gacaaatttc agatatcata caaaggaagc
      781 gttctctggg acccctttct atgtttagat ggttcactga acttgttcgt taacgtaaat
      841 atgtcttcct ctttgattcc aacaatgata ttataaggaa attatcgtca caatctaatc
      901 aacaaggaca aagaaaagaa aaagttgaga gagagaacaa aagttcaaat caaagaaaaa
      961 aaagaacatc aaattaccta ttacaataaa caattaagcc atactcgcaa caatttgcca
     1021 taatagaaag cagcacctcg ttggcgcagt cccttacata gtacacaaca tttatcactt
     1081 cacacaatca ggagtggaac tcaatggaaa acaacaccac acgtatgatc ttactaataa
     1141 aagaacatga acgttcctca gcgcgaacgt tcgcattctg cgccttcgag cacaggataa
     1201 gttgcaggaa gccatcacat ctatgcaacg attatcacga cacaaccttg ccgccgagaa
     1261 aacgtccgtg gagaaccatt cggtcgattg cttcccacgg aacgagtgga ctgaaactta
     1321 agactgcccc tctctttccg cggaatcgct catgccgcgg tgccacaaca ttttcagatt
     1381 tactaagact ccggtgagtg tgggctcacc cgcgggtcgt gatcacgatt cagcaccctt
     1441 tggactcgtg attccgtgga aaggtcagat ctgtattcct acttatggta atgtgctaaa
     1501 aaaagagaaa tgtctccgcg gaactcttct acgccgtggt acgatatctg ttgaacgtaa
     1561 tctgagcaat acaaacaagg cctctcctat acatatataa ttgtgatgtg cataacctta
     1621 tggctgttcg cttttattac cataccttag aatgaaatcc aaaagaaaaa agtcacgcaa
     1681 agttgcaaac atataacaac tgtgttagtt atcactcgac tttgttattc taattataaa
     1741 taaattggca actaggaact ttcgaaaaag aaattaaaga cccttttaag ttttcgtatc
     1801 cgctcgttcg aaagacttaa ttaagctttc ccatgtctct actggtgcgg ccgcgattat
     1861 aaagatgacg atgataaaca tcatcaccat catcattaaa ttcggccgct tctttggaat
     1921 tattggaagg taaggaattg ccaggtgttg ctttcttatc cgaaaagaaa taaattgaat
     1981 tgaattgaaa tcgatagatc aatttttttc ttttctcttt ccccatcctt tacgctaaaa
     2041 taatagttta ttttattttt tgaatatttt ttatttatat acgtatatat agactattat
     2101 ttatctttta atgattatta agatttttat taaaaaaaaa ttcgctcctc ttttaatgcc
     2161 tttatgcagt ttttttttcc cattcgatat ttctatgttc gggttcagcg tattttaagt
     2221 ttaataactc gaaaattctg cgttcgttgg atccttcagt gcttacaata ttgatgattg
     2281 ggataattgg gagcatctac ctatcggtat cgcaggtact gctgccttaa ttgttggtgc
     2341 cttcggtgtt gcattgggta tgtgtcaaac ctattgggtt ggtgaaattg gccgcttaat
     2401 tggtaaatat ggtggtgaca ttgggttcga gttgggtgca agttgggcct tcatcatcta
     2461 caacatctta agacctttag aattaaaata cttcggtcgt taggaagtaa tgattagggc
     2521 gatcatttcc ccgtgcacat ttcacgtcat gtatcataga tgcaatttca tgtaaactat
     2581 ttagacatct catttaataa aaatagctac aaactgtatt ttacaatatt agtaacatat
     2641 caaagataat ctctatggta tatacaaaac ttcaaaaatg ccaagacaat ttgcacggct
     2701 gcgacctaat tgagactttg tggcctgcat atcagtctta caataaaagc caaaaagaaa
     2761 aaaaaaaagt aagaatgcac aggtgactag tggttatgtg actatgtgaa cgtaaagaga
     2821 acgagatgta acccggcaac ccggctagag aggttgttta gcatgcgagg tattagactc
     2881 cttgtcaaaa aattataaaa tatttattag ttaatttatt agttgcaaag tcattgataa
     2941 cagcgtgact cttcccggaa attctttcat ttcggcattt cgaagcgaga ccgcaaaaca
     3001 cgtgtacgca cgtagccaac gattgaaaaa aaaagcttcc aagtgagtca cctctaccgt
     3061 tggaacttat aaaatatttt tgcagaatat actcgcctga aagtccattt atacgcagtg
     3121 aactgcaagt atttaccgtc taaactttgg cattttactc ctattaacgg tttgaatctt
     3181 tgaaaataga aaagaaagga taggtttcta aaaaattcaa tagaaaaatg gatttggtga
     3241 accatctaac cgatagacta ctgtttgcaa tcccaaagaa aggtcgttta tattctaaaa
     3301 gtgtttctat tttgaatggt gctgatatta cctttcaccg ctctcaaaga ttagacattg
     3361 cactaagcac aagcttacct gtagcgttgg tctttctgcc cgctgcagat attccaactt
     3421 ttgttggtga aggtaaatgt gatcttggta taactggtgt tgaccaagtt cgtgaatcta
     3481 acgtcgacgt agacttagca atcgatttgc aatttggtaa ctgtaaattg caggtacaag
     3541 tccccgtaaa tggcgagtat aaaaagccag aacagttaat tggcaaaacc attgttacca
     3601 gtttcgtgaa acttgctgaa aaatactttg ccgatttgga aggtactact gttgaaaaaa
     3661 tgaccacaag gataaagttt gtcagtggtt ccgtggaggc atcatgtgct ctgggaattg
     3721 gtgatgctat tgtagatctt gtagagagtg gtgagacaat gagggcagca ggtttagttg
     3781 atattgccac cgtcctaagc acaagtgcct acctaataga atcaaagaac ccaaagagcg
     3841 ataagagttt gattgctact atcaaatcaa gaattgaagg tgtcatgacc gctcaaaggt
     3901 tcgtttcatg tatttataac gcacctgaag acaagctgcc tgaactgttg aaggtgacgc
     3961 ctggccgtag agcaccaacc atttccaaaa ttgacgatga aggatgggtt gctgttagtt
     4021 ccatgattga gagaaaaacg aagggtgttg ttttagatga attgaaaaga ctcggcgcat
     4081 ctgatatcat ggttttcgaa atttctaatt gtcgtgtata agatagaaca gaaaaaggga
     4141 agccaaatcc cgattaattt tgtatttttt ataggcaata atttgataga gttctatctt
     4201 attctaagtt acccctcatg acttcccaag tgtgttttga ggttatctct gtgaatattt
     4261 tttctttttt tttgctactt ccccaggaag ttaaatcttc gtagcatatt tgtcagcttt
     4321 actaccgtct tcatgctcat gaaaccttct tcttcaaccc ccttcatttc tccaattttc
     4381 gaaaactgga gctacccata aatattcgga gttttgtagg tatttttatt ttggagaaaa
     4441 cctgtacaaa ctctccccag ggagcataga ttggggatcg taacgcataa agctgtctag
     4501 aattcacacc accatccggt atgacatgtg ggcagtacat ggaaccatat ttacaactag
     4561 caaagactgg ttacttaact gatgaaaatg ccactgacac ctgtagtttc tgtcaaatat
     4621 ctacaaccaa tgattactta gctaatgtca attctttcta cagtgagaga tggagaaatt
     4681 atggtatctt catctgttat attgcattca attatatcgc tggtgtcttt ttctactggt
     4741 tagcaagagt gcctaaaaag aacggtaggc gcgccaagtc tagtgcggcc ggccaagcgg
     4801 tggacctcga gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg
     4861 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     4921 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     4981 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     5041 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5101 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5161 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5221 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5281 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5341 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5401 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5461 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5521 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5581 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5641 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5701 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5761 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5821 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5881 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     5941 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6001 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6061 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6121 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6181 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6241 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6301 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6361 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6421 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     6481 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6541 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     6601 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     6661 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     6721 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6781 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     6841 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     6901 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     6961 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
