LOCUS       40924_3461        6975 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pABC4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Doehlemann J, Wagner M.
  TITLE     A family of single copy repABC-type shuttle vectors stably 
            maintained in the alpha-proteobacterium Sinorhizobium meliloti
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Doehlemann J, Wagner M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2016) LOEWE-Zentrum fuer Synthetische 
            Mikrobiologie, Philipps-Universitaet Marburg, Hans-Meerwein-Strasse,
            Marburg, Hesse 35043, Germany
REFERENCE   3  (bases 1 to 6975)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6975)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-OCT-2016) LOEWE-Zentrum fuer Synthetische Mikrobiologie, 
            Philipps-Universitaet Marburg, Hans-Meerwein-Strasse, Marburg, Hesse
            35043, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..4664
                     /label=oriVSm part (oriV_p42e) derived from pLoriV-42e
                     /note="oriVSm part (oriV_p42e) derived from pLoriV-42e"
     terminator      4720..4747
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      4885..4912
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      5049..5135
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    5219..5248
                     /label=MCS
                     /note="MCS"
     terminator      complement(5280..5307)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      5400..5988
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6081..6869)
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gaaactgtca ctggtccgtt gatacaatcc agtgagtgag accctcccct tcccctgtgg
       61 tcgccgtcag cgttgccagc cccgaggcgc cgagcgccca ggacggttcg ccggtccaga
      121 tgcgccgacg cgtccgcagc accgaatggg cgatcgtcag ttcatggctg ggagcgcggc
      181 tatccatatg cgcgtcgtgc agaacgagat cctcgacatg aacgagctcg ccggcgaccc
      241 ataacgcacc tgcggcatcg aagaagtggc tgcgctcggc aaatccctca ccaaccgatg
      301 agcgcaacac ccgctcgtcc agccgcgccg ccgcgtcctc cgccgcggca agcaatggaa
      361 gcagggtttg gagcattaca ccgtcgatat cgtagcgcat tgttaagctt tatagtttct
      421 cacaaatgga agcaaagcgg ctgtttcctt ccgccatcta tgacatggtt aaccgtttat
      481 ttcaacaaaa gcgcgggaag gaagagaaga agatggccga ctgccgttct acaatccccc
      541 taacagctgc gactggcgca aagctttcgg gcctttcatc ccgtttttct cgccatcgga
      601 ccattggccc gcatcaacgc catgagcatc ggaccgagcg agaattcacc gcgctctgcc
      661 cgccgcgtca gatcacgcat atagccgccc gccgaattga tatggccgcc gcgttccaga
      721 atgcaggcga tcactgtggc agcattttcc ggtcccatga catcgctggc ctgctgatag
      781 gcgctgggac tgacgcctag ggtggaacgg acgacgacgg cggcggccat caaatcccgc
      841 cagctgccga tcccgccgcc cggcccatag gcaacaatct cggggcaggc ctgcagcacc
      901 atggcaaggg ggaaggattt cggcggctcc cgccacggcc gcggttcttc ctccggcttt
      961 gcgccctgct tcgtttcgaa gcttggttca agttcagatg tgtggtgtgg gtttgaatta
     1021 tgtatgtgcc aaccgctctg gctgtcattg gcgtctgttt tttctgcttt cgtcttcgat
     1081 tccagcgttt tgatgatcag ttcgcggaac gcttcgagat cggtcagcag gctctggatc
     1141 tccgccgtcg atgggcggcg cggcaggctt gtcgagaggc gattgaaatg ctgctgcatc
     1201 tcgatccatt cgccggcaat ttcctcttcc agcgcgatct cgatgagctt ggcgatatca
     1261 cgccggcaaa ggctcagccg ctccctcagg cgcttccatt cgagcttgtc ggcaagcacc
     1321 tgcgccgcct gagcttcgat ctcgccagca cgcacgagga gtggcgccag gctgaagccg
     1381 aaggcttccc caagtccgcc ctcgccgttg cggcgggcaa aacgttttcc attcggactg
     1441 tcccgccgga tgatcaggcc ggcttccacc agcattgcca ggtttcgccg cagcgtggtg
     1501 ccggccatgc catgggcgcg cagagacagc tgctcattcg aaggaaagac gacgaagccg
     1561 ttggcttcgg taatctcgtt cttcggatag aaggtcaaca gcgcgttgag aacggcaagc
     1621 gcgcggtcgg acacgccgat cacgtctttt gcttcgcaga gcgcgcgaaa gatcttccat
     1681 ttgtcgaccg acgcgtccgg atcgatcctg ctggcccttt cctgacttgc cagcatgcca
     1741 agcgtcatcg accgccgccc gaagggcgtc gatacatatt gaggctccat gtccctcacc
     1801 ttttttcaag gcaaaagaaa tctaccaccc aaaaggatgc cgaagatgct tgacactgat
     1861 tcgaggaagt gcgattctca gattgtccag atatgagaaa ggcttccgcg acggcaacgt
     1921 tcggaggctt ttttcttttg cggcctactc tcctgcttgc aacttgccgg cctgataggc
     1981 ttcgtaaagc tcatcgagac ggcgtgaaat gtaagcgccg aaatccggcg catcgccggt
     2041 cttcaacgcg attgtaaagg aattgccggc gctcctgatt cggccggcaa tcttgccgcc
     2101 gctcttcggc ttccagtcat attcggtggg ctccggcttc gcctctttcc tggcgagttc
     2161 ggcgaccaga agttcgaacc ggcggtcact ctcctccgcc ctgaacccat ccgaggcgat
     2221 cagcttcgca agccgcgccg ccgtcgtccc attccagtcc tgagcaagtg ccatccactt
     2281 gcgtcgtccg atacccggtg cggcccccac cgacctgacg atctcggccg gaatggcctt
     2341 ggcgaccgac aacagcttcg acagctccgt cttatcagtg gacagcgcct tcatgatgac
     2401 cggacgctca aagcccttga gttcgagagt gagagcaaag acggcgcgtt cgatataaga
     2461 aagattccgg cgcgccgaat tctcgatgcc ctgcgcgata acgacatcgg tgtcgtcgag
     2521 ctgacggacg atcgcctgca ccttgcgctt gagcagcttg atagcctgta gccggcgatg
     2581 gccgtaggcg atctggtaac gctcaccgtc ttcgggatgg cgccgaacaa ggatcggcac
     2641 ttcctggcca ttgtcggcaa tcgactgcac cagctcatcc tcaagatcga gcggctggtc
     2701 ggcaaggcgg tcgcggacga aggaagaatc gatcagatcg ggatcgagtt cgacgatgcg
     2761 ctcgccggag agcaaagcct cctgcagcgc cctgctctct tcctccatgc ggccgagggt
     2821 cagcgccatt gtccgcaccg gaccggccga tccgcgcgaa gaaggctcct cgtagttggc
     2881 cgcggccaac tcctgcgccg tatcgtcgaa aagacctttc aggcgatcgc gtctgctcat
     2941 gtgcggcccc aggctttgtg aatgccggca agaacctcgc cattggcagc gttgaccgat
     3001 tccagcgccc ggtcatatgt cgagcggcgc acctgccctt tctcgatttc gtaaagcgtc
     3061 tgctttgtca gaccggcatc ggcgattgcc gtcgatttga gaatggttgc cgtcagcaca
     3121 tcgtcgccga acaggctgcg cagcagcgca acgatctgcg actgcggcgc atcgaagggt
     3181 tcgtggcggg tgacgacata tttgatgaag tcgtgctgca ggtcgccgcc ggccctgcga
     3241 acgacagaga gaagatccga ggtcatcagc aggaactgcg acatagaggc gacatcgacc
     3301 atctgcgggt ggatggtgat gagcagcgac gtggcggcgc aaacggcgcc gagagtgagg
     3361 tagccgagct gcggcggaca gtcgatcacc accacatcgt aatcggcctc cacctcggcg
     3421 atagcgacac cgacgcgacg gaaaaacagc tcggccggtt tctggcgatc actgagcgcc
     3481 cgcggcgtct catgctcgaa ttccatcagc tcgagattgc cgggcaccag atcgagtcca
     3541 tcgaaatagg tcttgcgaac gatctccttc agcgacttac gggccgcatc gtagcgaatg
     3601 gcgccataaa gcgtatcgcc gtcggaaaga tcgaattccg gctgaacgcc cagcatggag
     3661 gaaagcgagg cctgcgggtc gagatcaatc gccagcaccc ggtaaccctc cagcgccaga
     3721 tattgcgcca gatagagcgt cgtcgtcgtc ttggccgaac cgcccttgaa attggtgacg
     3781 gcgaccgtct gcagcttttc gcctcgacgt cgccacggga ggtagctcag cgcatccttc
     3841 ggcttgagat tcgccatata atggcgcaat tcattgatct gaccgagcgt ataggagcgt
     3901 cgaccattcg aggaaagttc cggctgcggc cccttgccgt ccagcgaaag ctgacgaaga
     3961 taaccgtcgg aaacgccgat catctgggcg gcttcgccgg aagagaatgt gcgcagcgtc
     4021 ttctgcgaaa gcggcggaaa cagcttgtcg cggagaagct tcagttgccg cgagagctgg
     4081 ttcgcatgcc gctcgatccg cacgtctgtc gtcgatacgc taatgttgtc caaaacaccc
     4141 aatccttttc gatgcgcttt tctgtcaaca tagcgtcaaa aagcgtatcg aaagtgacac
     4201 gattcgggat ttacggcaac aataggggtc aagccgccac ggcgggccga gttggccgcg
     4261 gccaacttcc tgccccgccc ttgcggccag cccggttttt cggcaagacc gcttggaccc
     4321 acggggaaat atccgcttga agcatattaa catcatcgga atcggcacgg gcaatccgga
     4381 gcacgtcact atgcaggcga tcaaagcgat gaacgccgcc gaggtgatct tcctgccgct
     4441 caagggcgcc gacaaggaag agctcgccga gatcaggcgg gagatctgcg agcgctacgt
     4501 cactcggcca gctgtccgaa ccgtggaatt ccaggtgccg caaaggcaga ccgcagacag
     4561 aacctacaca caaagtgtcg atgcctggca cggcgagatc gccgacatct atggagaatt
     4621 gatctcgggc cactaacttc tcgtggaact ctatcgtaac tgaagtctat tgaaggaaca
     4681 ctgtatctcg gtcaagtggg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
     4741 gccttctgct taatttgatg gtcgactcgg cggaaagcga gaagaggttc gcctcggtct
     4801 cgccggcggc gaatccggcc gtcgggccgg tcggcccctt ccggaagcga acgcgaaccg
     4861 tcttcaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttaatt
     4921 tgatggaact cggagcgtcc gcgtcctcgt aaacgagggc gagcggccgg ttaacggtcg
     4981 cggagtccga acggttcttc cgagttcgaa tccgcgggtc tccgggagcc ccgagataac
     5041 cggcgcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     5101 gtttgtcggt gaacgctctc ctgagtagga caaatccgcg atcccgtagc cgatcgcgcc
     5161 gaagacctcc gcgccgccgg agcccgaact ctcggcgagg aagccgaccg tcgtcggggt
     5221 ttaaacttaa ttaagcggcc gcagtactca tatgcccact aagagtttgt agaaacgcaa
     5281 aaaggccatc cgtcaggatg gccttctgct taatttgatg gttatcagaa gagtgtaagc
     5341 gggttgacta tactactgtt tcagactggc gtaatcactc agtagatcaa aggatcttct
     5401 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5461 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5521 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     5581 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5641 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5701 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5761 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     5821 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5881 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5941 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6001 gcgactggca atcactgagt aagactattc tgtccagacc ttttctccga cgaatagagt
     6061 aacagaagtc gggatcctta tttgccgact accttggtga tctcgccttt cacgtagtgg
     6121 acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa
     6181 gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag
     6241 tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac
     6301 aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt
     6361 aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc
     6421 gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga
     6481 tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct
     6541 ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca
     6601 atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa
     6661 aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc
     6721 aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg
     6781 gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc
     6841 gatacttcgg cgatcaccgc ttccctcata tgttttctcc tctgctacgt aacgcgtcta
     6901 cttgcgtact agttaactag ttcgatgatt aattgtcaac agactgattg gtcaggcaca
     6961 actttcagat aagac
//
