LOCUS       40924_3446        5818 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pABC3-HisCys, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Lamping E, Monk BC, Niimi K, Holmes AR, Tsao S, Tanabe K, Niimi M, 
            Uehara Y, Cannon RD.
  TITLE     Characterization of three classes of membrane proteins involved in 
            fungal azole resistance by functional hyperexpression in 
            Saccharomyces cerevisiae
  JOURNAL   Eukaryotic Cell 6 (7), 1150-1165 (2007)
  PUBMED    17513564
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Lamping E, Monk BC, Niimi K, Holmes AR, Tsao S, Kaneko A, Niimi M, 
            Uehara Y, Cannon RD.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2006) Oral Sciences, Univ. of Otago, 310 Gt. King 
            St, Dunedin, Otago 9001, New Zealand
REFERENCE   3  (bases 1 to 5818)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5818)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic 
            Cell"; date: "2007"; volume: "6"; issue: "7"; pages: "1150-1165"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-AUG-2006) Oral Sciences, Univ. of Otago, 310 Gt. King St, 
            Dunedin, Otago 9001, New Zealand"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      687..1816
                     /label=Saccharomyces cerevisiae PDR5
                     /note="Saccharomyces cerevisiae PDR5"
                     /regulatory_class="promoter"
     misc_feature    1817..1854
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             1855..1872
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             1873..1890
                     /label=tetracysteine tag
                     /note="tetracysteine peptide that binds biarsenical
                     labeling reagents"
     terminator      1968..2238
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate
                     kinase gene"
     promoter        2274..2485
                     /label=URA3 promoter
     CDS             2486..3286
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     misc_feature    3309..3576
                     /label=Saccharomyces cerevisiae PDR5 gene, 3' end
                     /note="Saccharomyces cerevisiae PDR5 gene, 3' end"
     promoter        complement(3630..3648)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3669..3685)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3693..3709)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3717..3747)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3762..3783)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4071..4659)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4833..5690)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5691..5795)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccgggcc
      661 ccccctgcag gtcaacggta tcgatggtcc gtcatatacg gttctccgtt gggcgcgccc
      721 acacacatat atataagcct gaatgcagta gacaaatttc agatatcata caaaggaagc
      781 gttctctggg acccctttct atgtttagat ggttcactga acttgttcgt taacgtaaat
      841 atgtcttcct ctttgattcc aacaatgata ttataaggaa attatcgtca caatctaatc
      901 aacaaggaca aagaaaagaa aaagttgaga gagagaacaa aagttcaaat caaagaaaaa
      961 aaagaacatc aaattaccta ttacaataaa caattaagcc atactcgcaa caatttgcca
     1021 taatagaaag cagcacctcg ttggcgcagt cccttacata gtacacaaca tttatcactt
     1081 cacacaatca ggagtggaac tcaatggaaa acaacaccac acgtatgatc ttactaataa
     1141 aagaacatga acgttcctca gcgcgaacgt tcgcattctg cgccttcgag cacaggataa
     1201 gttgcaggaa gccatcacat ctatgcaacg attatcacga cacaaccttg ccgccgagaa
     1261 aacgtccgtg gagaaccatt cggtcgattg cttcccacgg aacgagtgga ctgaaactta
     1321 agactgcccc tctctttccg cggaatcgct catgccgcgg tgccacaaca ttttcagatt
     1381 tactaagact ccggtgagtg tgggctcacc cgcgggtcgt gatcacgatt cagcaccctt
     1441 tggactcgtg attccgtgga aaggtcagat ctgtattcct acttatggta atgtgctaaa
     1501 aaaagagaaa tgtctccgcg gaactcttct acgccgtggt acgatatctg ttgaacgtaa
     1561 tctgagcaat acaaacaagg cctctcctat acatatataa ttgtgatgtg cataacctta
     1621 tggctgttcg cttttattac cataccttag aatgaaatcc aaaagaaaaa agtcacgcaa
     1681 agttgcaaac atataacaac tgtgttagtt atcactcgac tttgttattc taattataaa
     1741 taaattggca actaggaact ttcgaaaaag aaattaaaga cccttttaag ttttcgtatc
     1801 cgctcgttcg aaagacttaa ttaagctttc ccatgtctct actggtgcgg ccgccatcat
     1861 caccatcatc attgttgtcc aggttgctgt taaattcggc cgcttctttg gaattattgg
     1921 aaggtaagga attgccaggt gttgctttct tatccgaaaa gaaataaatt gaattgaatt
     1981 gaaatcgata gatcaatttt tttcttttct ctttccccat cctttacgct aaaataatag
     2041 tttattttat tttttgaata ttttttattt atatacgtat atatagacta ttatttatct
     2101 tttaatgatt attaagattt ttattaaaaa aaaattcgct cctcttttaa tgcctttatg
     2161 cagttttttt ttcccattcg atatttctat gttcgggttc agcgtatttt aagtttaata
     2221 actcgaaaat tctgcgttcg ttggatccgg tgattgattg agcaagctag cttttcaatt
     2281 catcattttt tttttattct tttttttgat ttcggtttcc ttgaaatttt tttgattcgg
     2341 taatctccga acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac
     2401 gcatatgtag tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa
     2461 caaaaaccgg aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac
     2521 tcatcctagt cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt
     2581 gtgtgcttca ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg
     2641 tcccaaaatt tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg
     2701 cacagttaag ccgctaaagg cattatccgc caagtacaat tttttactct tcgaagacag
     2761 aaaatttgct gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat
     2821 agcagaatgg gcagacatta cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg
     2881 tttgaagcag gcggcagaag aagtaacaaa ggaacctaga ggccttttga tgttagcaga
     2941 attgtcatgc aagggctccc tatctactgg agaatatact aagggtactg ttgacattgc
     3001 gaagagcgac aaagattttg ttatcggctt tattgctcaa agagacatgg gtggaagaga
     3061 tgaaggttac gattggttga ttatgacacc cggtgtgggt ttagatgaca agggagacgc
     3121 attgggtcaa cagtatagaa ccgtggatga tgtggtctct acaggatctg acattattat
     3181 tgttggaaga ggactatttg caaagggaag ggatgctaag gtagagggtg aacgttacag
     3241 aaaagcaggc tgggaagcat atttgagaag atgcggccag caaaactaaa aaactgtatt
     3301 ataagtaaga attcacacca ccatccggta tgacatgtgg gcagtacatg gaaccatatt
     3361 tacaactagc aaagactggt tacttaactg atgaaaatgc cactgacacc tgtagtttct
     3421 gtcaaatatc tacaaccaat gattacttag ctaatgtcaa ttctttctac agtgagagat
     3481 ggagaaatta tggtatcttc atctgttata ttgcattcaa ttatatcgct ggtgtctttt
     3541 tctactggtt agcaagagtg cctaaaaaga acggtaggcg cgccaagtct agtgcggccg
     3601 gccaagcggt ggacctcgag cttttgttcc ctttagtgag ggttaatttc gagcttggcg
     3661 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     3721 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     3781 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     3841 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     3901 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     3961 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4021 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4081 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4141 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4201 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4261 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4321 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4381 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4441 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4501 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4561 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4621 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4681 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4741 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4801 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4861 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4921 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     4981 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5041 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     5101 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5161 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5221 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     5281 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5341 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5401 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     5461 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5521 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5581 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5641 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5701 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5761 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
