LOCUS       40924_3436        6495 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pABC3-Gfp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Lamping E, Monk BC, Niimi K, Holmes AR, Tsao S, Tanabe K, Niimi M, 
            Uehara Y, Cannon RD.
  TITLE     Characterization of three classes of membrane proteins involved in 
            fungal azole resistance by functional hyperexpression in 
            Saccharomyces cerevisiae
  JOURNAL   Eukaryotic Cell 6 (7), 1150-1165 (2007)
  PUBMED    17513564
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Lamping E, Monk BC, Niimi K, Holmes AR, Tsao S, Kaneko A, Niimi M, 
            Uehara Y, Cannon RD.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2006) Oral Sciences, Univ. of Otago, 310 Gt. King 
            St, Dunedin, Otago 9001, New Zealand
REFERENCE   3  (bases 1 to 6495)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6495)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic 
            Cell"; date: "2007"; volume: "6"; issue: "7"; pages: "1150-1165"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-AUG-2006) Oral Sciences, Univ. of Otago, 310 Gt. King St, 
            Dunedin, Otago 9001, New Zealand"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      687..1816
                     /label=Saccharomyces cerevisiae PDR5
                     /note="Saccharomyces cerevisiae PDR5"
                     /regulatory_class="promoter"
     misc_feature    1817..1854
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             1855..2568
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     terminator      2645..2915
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate
                     kinase gene"
     promoter        2951..3162
                     /label=URA3 promoter
     CDS             3163..3963
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     misc_feature    3986..4253
                     /label=Saccharomyces cerevisiae PDR5 gene, 3' end
                     /note="Saccharomyces cerevisiae PDR5 gene, 3' end"
     promoter        complement(4307..4325)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4346..4362)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4370..4386)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4394..4424)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4439..4460)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4748..5336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5510..6367)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6368..6472)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccgggcc
      661 ccccctgcag gtcaacggta tcgatggtcc gtcatatacg gttctccgtt gggcgcgccc
      721 acacacatat atataagcct gaatgcagta gacaaatttc agatatcata caaaggaagc
      781 gttctctggg acccctttct atgtttagat ggttcactga acttgttcgt taacgtaaat
      841 atgtcttcct ctttgattcc aacaatgata ttataaggaa attatcgtca caatctaatc
      901 aacaaggaca aagaaaagaa aaagttgaga gagagaacaa aagttcaaat caaagaaaaa
      961 aaagaacatc aaattaccta ttacaataaa caattaagcc atactcgcaa caatttgcca
     1021 taatagaaag cagcacctcg ttggcgcagt cccttacata gtacacaaca tttatcactt
     1081 cacacaatca ggagtggaac tcaatggaaa acaacaccac acgtatgatc ttactaataa
     1141 aagaacatga acgttcctca gcgcgaacgt tcgcattctg cgccttcgag cacaggataa
     1201 gttgcaggaa gccatcacat ctatgcaacg attatcacga cacaaccttg ccgccgagaa
     1261 aacgtccgtg gagaaccatt cggtcgattg cttcccacgg aacgagtgga ctgaaactta
     1321 agactgcccc tctctttccg cggaatcgct catgccgcgg tgccacaaca ttttcagatt
     1381 tactaagact ccggtgagtg tgggctcacc cgcgggtcgt gatcacgatt cagcaccctt
     1441 tggactcgtg attccgtgga aaggtcagat ctgtattcct acttatggta atgtgctaaa
     1501 aaaagagaaa tgtctccgcg gaactcttct acgccgtggt acgatatctg ttgaacgtaa
     1561 tctgagcaat acaaacaagg cctctcctat acatatataa ttgtgatgtg cataacctta
     1621 tggctgttcg cttttattac cataccttag aatgaaatcc aaaagaaaaa agtcacgcaa
     1681 agttgcaaac atataacaac tgtgttagtt atcactcgac tttgttattc taattataaa
     1741 taaattggca actaggaact ttcgaaaaag aaattaaaga cccttttaag ttttcgtatc
     1801 cgctcgttcg aaagacttaa ttaagctttc ccatgtctct actggtgcgg ccgcatgtct
     1861 aaaggtgaag aattattcac tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt
     1921 aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg atgctactta cggtaaattg
     1981 accttaaaat ttatttgtac tactggtaaa ttgccagttc catggccaac cttagtcact
     2041 actttcggtt atggtgttca atgttttgcg agatacccag atcatatgaa acaacatgac
     2101 tttttcaagt ctgccatgcc agaaggttat gttcaagaaa gaactatttt tttcaaagat
     2161 gacggtaact acaagaccag agctgaagtc aagtttgaag gtgatacctt agttaataga
     2221 atcgaattaa aaggtattga ttttaaagaa gatggtaaca ttttaggtca caaattggaa
     2281 tacaactata actctcacaa tgtttacatc atggctgaca aacaaaagaa tggtatcaaa
     2341 gttaacttca aaattagaca caacattgaa gatggttctg ttcaattagc tgaccattat
     2401 caacaaaata ctccaattgg tgatggtcca gtcttgttac cagacaacca ttacttatcc
     2461 actcaatctg ccttatccaa agatccaaac gaaaagagag accacatggt cttgttagaa
     2521 tttgttactg ctgctggtat tacccatggt atggatgaat tgtacaaata aatcggccgc
     2581 ttctttggaa ttattggaag gtaaggaatt gccaggtgtt gctttcttat ccgaaaagaa
     2641 ataaattgaa ttgaattgaa atcgatagat caattttttt cttttctctt tccccatcct
     2701 ttacgctaaa ataatagttt attttatttt ttgaatattt tttatttata tacgtatata
     2761 tagactatta tttatctttt aatgattatt aagattttta ttaaaaaaaa attcgctcct
     2821 cttttaatgc ctttatgcag tttttttttc ccattcgata tttctatgtt cgggttcagc
     2881 gtattttaag tttaataact cgaaaattct gcgttcgttg gatccggtga ttgattgagc
     2941 aagctagctt ttcaattcat catttttttt ttattctttt ttttgatttc ggtttccttg
     3001 aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg agcacagact
     3061 tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt
     3121 aacccaactg cacagaacaa aaaccggaaa cgaagataaa tcatgtcgaa agctacatat
     3181 aaggaacgtg ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac
     3241 gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag
     3301 ttagttgaag cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact
     3361 gatttttcca tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt
     3421 ttactcttcg aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct
     3481 gcgggtgtat acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc
     3541 ccaggtattg ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc
     3601 cttttgatgt tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag
     3661 ggtactgttg acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga
     3721 gacatgggtg gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta
     3781 gatgacaagg gagacgcatt gggtcaacag tatagaaccg tggatgatgt ggtctctaca
     3841 ggatctgaca ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta
     3901 gagggtgaac gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa
     3961 aactaaaaaa ctgtattata agtaagaatt cacaccacca tccggtatga catgtgggca
     4021 gtacatggaa ccatatttac aactagcaaa gactggttac ttaactgatg aaaatgccac
     4081 tgacacctgt agtttctgtc aaatatctac aaccaatgat tacttagcta atgtcaattc
     4141 tttctacagt gagagatgga gaaattatgg tatcttcatc tgttatattg cattcaatta
     4201 tatcgctggt gtctttttct actggttagc aagagtgcct aaaaagaacg gtaggcgcgc
     4261 caagtctagt gcggccggcc aagcggtgga cctcgagctt ttgttccctt tagtgagggt
     4321 taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4381 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4441 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     4501 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     4561 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     4621 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4681 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4741 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4801 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4861 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4921 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4981 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5041 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5101 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5161 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     5221 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5281 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5341 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5401 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     5461 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     5521 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     5581 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     5641 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     5701 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     5761 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     5821 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     5881 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     5941 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6001 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6061 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6121 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6181 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6241 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6301 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6361 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6421 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     6481 cccgaaaagt gccac
//
