LOCUS       40924_3406        6869 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pABC1-sT2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Doehlemann J, Wagner M.
  TITLE     A family of single copy repABC-type shuttle vectors stably 
            maintained in the alpha-proteobacterium Sinorhizobium meliloti
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   Doehlemann J, Wagner M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2016) LOEWE-Zentrum fuer Synthetische 
            Mikrobiologie, Philipps-Universitaet Marburg, Hans-Meerwein-Strasse,
            Marburg, Hesse 35043, Germany
REFERENCE   3  (bases 1 to 6869)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6869)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-OCT-2016) LOEWE-Zentrum fuer Synthetische Mikrobiologie, 
            Philipps-Universitaet Marburg, Hans-Meerwein-Strasse, Marburg, Hesse
            35043, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..4558
                     /label=oriVSm part (oriV_p42b) derived from pLoriV-42b
                     /note="oriVSm part (oriV_p42b) derived from pLoriV-42b"
     terminator      4614..4641
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      4779..4806
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      4943..5029
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    5113..5142
                     /label=MCS
                     /note="MCS"
     terminator      complement(5174..5201)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      5294..5882
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5975..6763)
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gaaactgtca ctggtccgtt gatacaatcc agtcgtcaaa aaggcttttc acgtcaactc
       61 caaccgcacc gtttctccag cgaacgccgg ccgatcgtcg atcaggtcct tccctcgcct
      121 tgccaatcaa aaatcttgta acttcatgat ctcaaaggaa gaatatccta acaagaagtt
      181 atcaccctgt ttatttccgt cacctttcgt taaccataga tcgccacgga cagatgacac
      241 gaaatttttc atccggtctt gcctctgatt ggcgaattgg tgcggacaag cgccatcaac
      301 atcggtccga tggcaaattc gcctctctcg gcgcggcggg tcaggtcacg gaggtaacca
      361 ccggcggaat tgatgtgccc tgccctttca agcacgcacg ctatgacggt agccgcattt
      421 tcaggcccca tgacctcgca ggcctgttca taggccgagg ggctcactcc gagcattgag
      481 cgcacgacga cagccgccgc catcagatcg cgccatgtgc ccaccgagcc ctgcggtcca
      541 taggcggcga tctctgggca ggcctgcagc accaggccca gcgggaagga tttgagcccg
      601 ccgccggcaa ccgaggctga gtttgcagct ggcatgggcc tgcccttccc ctcctgtgtc
      661 gctattctgg ccctcggctc ggcccaacct tccgattggt cgtccaccgt tgcgccctgc
      721 ttcgtttcga agcgtggttc aagttcagta gtgtagtgtg ggtttgaatt ctgtatgtgc
      781 cgctcagaat gttgggcatt gccgctttga tttggagctt taacctgcat ttccaactga
      841 ttggtgattt cttcgcgcaa cagttccagt tcgtcgagtg cggcggcaat cttctcgaag
      901 gatggcgagc gcggcagccg ttcgacgatg cctcgaaaat gcaggtggac cccgctccaa
      961 tcaccagagg cgccctcttc cacggccatc tcgatgagct tggcgatgtc gcgcctgcaa
     1021 agcgtcaggc gctctttgag ccgctgcagc tgcaatcgtt cggctgccat ctcggccgcc
     1081 agccgctcga tctcgtccgc gcgcgcaagc agcggcgcca gcgagaagcc gaatgcctca
     1141 cggatctccc cgccctgctc cttgcgagcg taacgcttgc cgttcgggct gtccttgcgg
     1201 atcagcaatc cggcctcgat cagcgccgcc aggtggcgac ggatcgtctg ctccgccatt
     1261 ccatgcgcgc gcagagagag ctgcgtattc gaaggaaata cgaccaagcc gttctcctcg
     1321 gagagctctc ccttgggata aaagctcagc aaggcgttca ggatggaaag cgcccggtct
     1381 gtaatgccga gcagcggcct ggcctcgcac agggcgcgat agagtttcca tttatcaatc
     1441 gatcggccgg gctcgatttt tcgggcagcg gcctgacttg ccaacatgcc aaacgtcatc
     1501 ggccgccgcc cgaagggcgt cgtcacactt ccgctttcca ttttcttcgc cttcttcaag
     1561 gcaaaagatc acgcctcacc aaattcggtg ccaaagactc ttgactatga ttcgcggaaa
     1621 tgtgattctc tgggtgtcta gatcagagag ggcttccgcg acggcaacgt tcgggggcct
     1681 tttcttttgc ttattgatct ccgttcttcg tcaaatcctg tgtcttctcg aacgcctcgt
     1741 agagacgatc cagattgctg gcgatgtagg cgccaaaggc agatgccttc tccgccttca
     1801 gcgcgatggt gaactgtttt ccatcatcct tgatcttcgc tttgacggct ccgtcccggc
     1861 gctgccaggc gtgcgccacg ggctgaactg cggcggcggg cgacgcctgt tggttcctgc
     1921 tgatgaaggc gacaagcatg tcgaagcggg cgtccggctc ggccgtctcg aactccaccg
     1981 atctggtgaa ctcaatcgcc cgggctgcga tgttttccgt ctcgaatttc gccgaaagat
     2041 catgccagcg atcgcggcca gtcgtcttgg ctacgccgat cgcggccagg acttcggccg
     2101 gaatccgctt cgtgaccgac agcatcttgg acaccgtcgt cttgtctgcg ctgagtgccg
     2161 acatgatcgt ttcccggtcg aaacccaacg tgtcgagctt gtcggcaaac atcgtcctct
     2221 cgatgaagga gagatcggcg cgtgccgaat tctcctggcc ctgcgcaatg acatggtcgc
     2281 ggtcgtcgag tctcttgacc accgcccgga caggtcggcc gagctccttt gccgcccgcg
     2341 cacgacgatg accaaaagcg atctgatatc gcccctccct ctcgggatgc gggcgcacaa
     2401 ggatcggtga atcctggccg cgcaggcgga tcgcctcgac taattcccgg aactgttcgt
     2461 cagtatgcgc cagccggtcg gtcacgaagg aatcttcgat cagcgccggg tcgagatcga
     2521 tcaccgtctc gccgctcgcc agctgctctt cgatcgcctt ggcggcatcg gccttggcgg
     2581 ccaaggcatc gatgctgcgg gtgaccgcac cgagcgcgcc aatcccctta taggtgatat
     2641 gctgcctctc gtcgcgaggg gccggctcat cctcattgtt taccgcagta aacttcttgg
     2701 aatcgtccat caggcccgac agaagatttt tacgcgccat caaccagctc cttgccccgg
     2761 tttaccgcag taaacttccc gctcatttcc ggccccatgc cttatggatg agatcgacga
     2821 tttccgtgtt gactgactcc atcgcctcca tggcgcggtc ataggtcgcg cgggtaaact
     2881 gacttcgctc gacttcgtac agcgtctggt tagtcatcgc cgcatcggag atcgcgacgc
     2941 tgcgcagcat cgggttggtg agcacatggc tcttgaacat cgagcgcatg aaggcgacca
     3001 tctgcgtctg cggcccgtcc tgcggatcgt agcgggtcac gaggtaacgc agccagtcga
     3061 ggttcatatt gccgccggct cccttcagcg tgttcagcac ttcgccaagc atttgcagga
     3121 actggcacat cgacatgaca tcgagcatct gcggatgaac ggtgatcaga accgccgtcg
     3181 caccgcaaat ggcgctcatg gtcaaaaagc caagctgggg agggcaatcg atgatgacga
     3241 cgtcgtaatc atccgcgacc gaggccagcg cctcatccag ccgggcaaag aagacgcgcc
     3301 cataatcgct cgctttgccc tgagcgagga cccgcggcgt atcgtgctcg aattccatca
     3361 gttcgagatt gcccggcacg agatgaaggt tcgggaagtt ggtcggccgg ataatgtccc
     3421 gcagcggacg ccgctgatcg tcatagcgaa tggcggcgta gagtgtctcg ttttcgttga
     3481 cgtcgaactc cggctgaaag ccgtgtatgg cagaaagaga cgcttgcgga tcgagatcga
     3541 cggccaggac gcggtgaccg gtcagggcga gatgctgggc aagatgcgcg gcactcgtcg
     3601 tcttgccgct gccccccttg aagttgacga cagcaacgac ctgcagatgt tcgtggccgc
     3661 ggcgatgacg cacataatgt gttccggcgc gcgcattatg ctcgagaaag ccacgcatct
     3721 cctccatctg ctccgccgta tacgatcggc ggccagacgg cgtaacctgg gggagggcac
     3781 ccttgccctc gagcgaaaga tttcgaagat agccgctggt cacgccgaga aaccgcgcca
     3841 cttcagcaag ctgaaattcc cgcagaccct ttagcgcgcg cggaggaaac atttcgaggc
     3901 gatgctgctt cagcttgtcg gatagttcct gtgcctgacc gatgatcagc tggtcgacat
     3961 cggaaattgc tttttctatt tgcggtgcca tattcatgga aatctcgaaa atgcgattta
     4021 aaagctctac gcgctttgaa accgcattta gcgccgattc tggaggggtg gcaaggcaaa
     4081 tagggttaac aagatcctat caaaaggttg tccagtctat ttcaagcgct tgcgccggcc
     4141 gggcgtgccg ctggcgggtc cagcaatcgc acattcacag gccgaatcgg acgagaccgg
     4201 ccgggaacag cagcagtata atccgaatcg caatgtcggg cgtgcaaagc gccttgctgc
     4261 aagcccgcag atgcaaggca tcggttcgct ggcgtggatg gagcggggcg cggcggtaag
     4321 gtgccgggtc tggtcgatca gccgtaactt gccagatcat tcgagcctga gttcacaggg
     4381 gaattgccgc ctggccagct actccagagg cggacgggtg atattcgcta tcgaaggcaa
     4441 agccggaccg ctttccgcct gcgccatcgc ttttaccaat gcgatgatcg tttcacgaat
     4501 atcgggatcg gcaatcttca gaaagactaa cttctcgtgg aactctatcg taactgaagt
     4561 ctattgaagg aacactgtat ctcggtcaag tgggaagagt ttgtagaaac gcaaaaaggc
     4621 catccgtcag gatggccttc tgcttaattt gatggtcgac aggggctcgg cgcttagagc
     4681 ctctcttagc gaccgctcta gaccgcgacc gcgttcggac tcgcgacgga ctacgagtag
     4741 tagaccctcg cccggaagca agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     4801 ccttctgctt aatttgatgg cgaggccccg gagcggaccg gcgacgaccg ggaggccgag
     4861 gattacggtc tccttagtcc ggcttaggag gagacctccg ccgcgctcct cctcgcggag
     4921 gagttcggcg cctagacgac atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     4981 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc gggcggcttc
     5041 tcgccgataa tcgggctcgg cctcgccctc gcggagctcc tcgattagac cgtcgtttag
     5101 aagctcgtcg aggtttaaac ttaattaagc ggccgcagta ctcatatgcc cactaagagt
     5161 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttaattt gatggttatc
     5221 agaagagtgt aagcgggttg actatactac tgtttcagac tggcgtaatc actcagtaga
     5281 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5341 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5401 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     5461 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5521 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5581 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5641 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     5701 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5761 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5821 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5881 aaaacgccag caacgcgact ggcaatcact gagtaagact attctgtcca gaccttttct
     5941 ccgacgaata gagtaacaga agtcgggatc cttatttgcc gactaccttg gtgatctcgc
     6001 ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt
     6061 cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc
     6121 gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt
     6181 accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg
     6241 agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat
     6301 caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca
     6361 gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat
     6421 tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt
     6481 cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag
     6541 ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg
     6601 tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc
     6661 cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct
     6721 ctgatagttg agtcgatact tcggcgatca ccgcttccct catatgtttt ctcctctgct
     6781 acgtaacgcg tctacttgcg tactagttaa ctagttcgat gattaattgt caacagactg
     6841 attggtcagg cacaactttc agataagac
//
