LOCUS       40924_3366        7313 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Yeast two-hybrid vector pAB8, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7313)
  AUTHORS   Guo D, Hazbun TR, Xu XJ, Ng SL, Fields S, Kuo MH.
  TITLE     A tethered catalysis, two-hybrid system to identify protein-protein 
            interactions requiring post-translational modifications
  JOURNAL   Nat. Biotechnol. 22 (7), 888-892 (2004)
  PUBMED    15208639
REFERENCE   2  (bases 1 to 7313)
  AUTHORS   Kuo M-H., Guo D, Ng S-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2004) Biochem. Mol. Biol., Michigan State 
            University, 309 BCH Building, East Lansing, MI 48824, USA
REFERENCE   3  (bases 1 to 7313)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7313)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Biotechnol."; date: "2004"; volume: "22"; issue: "7"; pages: 
            "888-892"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JUN-2004) Biochem. Mol. Biol., Michigan State University, 309 
            BCH Building, East Lansing, MI 48824, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             434..874
                     /codon_start=1
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             974..1063
                     /codon_start=1
                     /label=3xHA
                     /note="three tandem HA epitope tags"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      1502..1689
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    complement(2521..3683)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the
                     autonomously replicating sequence ARS1/ARS416"
     promoter        complement(4197..4298)
                     /label=TRP1 promoter
     promoter        4404..4508
                     /label=AmpR promoter
     CDS             4509..5366
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5540..6128
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtggtacata acgaactaat actgtagccc tagacttgat agccatcatc atatcgaagt
       61 ttcactaccc tttttccatt tgccatctat tgaagtaata ataggcgcat gcaacttctt
      121 ttcttttttt ttcttttctc tctcccccgt tgttgtctca ccatatccgc aatgacaaaa
      181 aaatgatgga agacactaaa ggaaaaaatt aacgacaaag acagcaccaa cagatgtcgt
      241 tgttccagag ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt ccttcattga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gcttgaagca
      421 agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact
      481 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg
      541 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga
      601 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga
      661 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt
      721 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac
      781 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag
      841 tagtaacaaa ggtcaaagac agttgactgt atcgaactat ctattcgatg atgaagatac
      901 cccaccaaac ccaaaaaaag agatcgaatt ccagctgacc accatggcaa ttcccggtgg
      961 cggccgcatc ttttacccat acgatgttcc tgactatgcg ggctatccct atgacgtccc
     1021 ggactatgca ggatcctatc catatgacgt tccagattac gctgctcagt gcggccgctc
     1081 tagctagaac tagtggatcc cccgataccg tcgacctgca gagatctatg aatcgtagat
     1141 actgaaaaac cccgcaagtt cacttcaact gtgcatcgtg caccatctca atttctttca
     1201 tttatacatc gttttgcctt cttttatgta actatactcc tctaagtttc aatcttggcc
     1261 atgtaacctc tgatctatag aattttttaa atgactagaa ttaatgccca tctttttttt
     1321 ggacctaaat tcttcatgaa aatatattac gagggcttat tcagaagctt tggacttctt
     1381 cgccagaggt ttggtcaagt ctccaatcaa ggttgtcggc ttgtctacct tgccagaaat
     1441 ttacgaaaag atggaaaagg gtcaaatcgt tggtagatac gttgttgaca cttctaaata
     1501 agcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt
     1561 atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc
     1621 tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac
     1681 ctctaccggc atgccgagca aatgcctgca aatcgctccc catttcaccc aattgtagat
     1741 atgctaactc cagcaatgag ttgatgaatc tcggtgtgta ttttatgtcc tcagaggaca
     1801 acacctgttg taatcgttct tccacacgga tcctggcgta atagcgaaga ggcccgcacc
     1861 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     1921 ctccttacgc atctgtgcgg tatttcacac cgcatatatc gctgggccat tctcatgaag
     1981 aatatcttga atttattgtc atattactag ttggtgtgga agtccatata tcggtgatca
     2041 atatagtggt tgacatgctg gctagtcaac attgagcctt ttgatcatgc aaatatatta
     2101 cggtatttta caatcaaata tcaaacttaa ctattgactt tataacttat ttaggtggta
     2161 acattcttat aaaaaagaaa aaaattactg caaaacagta ctagctttta acttgtatcc
     2221 taggttatct atgctgtctc accatagaga atattaccta tttcagaatg tatgtccatg
     2281 attcgccggg taaatacata taatacacaa atctggctta ataaagtcta taatatatct
     2341 cataaagaag tgctaaattg gctagtgcta tatattttta agaaaatttc ttttgactaa
     2401 gtccatatcg actttgtaaa agttcacttt agcatacata tattacacga gccagaaatt
     2461 gtaacttttg cctaaaatca caaattgcaa aatttaattg cttgcaaaag gtcacatgct
     2521 tataatcaac ttttttaaaa atttaaaata cttttttatt ttttattttt aaacataaat
     2581 gaaataattt atttattgtt tatgattacc gaaacataaa acctgctcaa gaaaaagaaa
     2641 ctgttttgtc cttggaaaaa aagcactacc taggagcggc caaaatgccg aggctttcat
     2701 agcttaaact ctttacagaa aataggcatt atagatcagt tcgagttttc ttattcttcc
     2761 ttccggtttt atcgtcacag ttttacagta aataagtatc acctcttaga gttcgatgat
     2821 aagctgtcaa acatgagaat taattccaca tgttaaaata gtgaaggagc atgttcggca
     2881 cacagtggac cgaacgtggg gtaagtgcac tagggtccgg ttaaacggat ctcgcattga
     2941 tgaggcaacg ctaattatca acatatagat tgttatctat ctgcatgaac acgaaatctt
     3001 tacttgacga cttgaggctg atggtgttta tgcaaagaaa ccactgtgtt taatatgtgt
     3061 cactgtttga tattactgtc agcgtagaag ataatagtaa aagcggttaa taagtgtatt
     3121 tgagataagt gtgataaagt ttttacagcg aaaagacgat aaatacaaga aaatgattac
     3181 gaggatacgg agagaggtat gtacatgtgt atttatatac taagctgccg gcggttgttt
     3241 gcaagaccga gaaaaggcta gcaagaatcg ggtcattgta gcgtatgcgc ctgtgaacat
     3301 tctcttcaac aagtttgatt ccattgcggt gaaatggtaa aagtcaaccc cctgcgatgt
     3361 atattttcct gtacaatcaa tcaaaaagcc aaatgattta gcattatctt tacatcttgt
     3421 tattttacag attttatgtt tagatctttt atgcttgctt ttcaaaaggc ttgcaggcaa
     3481 gtgcacaaac aatacttaaa taaatactac tcagtaataa cctatttctt agcatttttg
     3541 acgaaatttg ctattttgtt agagtctttt acaccatttg tctccacacc tccgcttaca
     3601 tcaacaccaa taacgccatt taatctaagc gcatcaccaa cattttctgg cgtcagtcca
     3661 ccagctaaca taaaatgtaa gctctcgggg ctctcttgcc ttccaaccca gtcagaaatc
     3721 gagttccaat ccaaaagttc acctgtccca cctgcttctg aatcaaacaa gggaataaac
     3781 gaatgaggtt tctgtgaagc tgcactgagt agtatgttgc agtcttttgg aaatacgagt
     3841 cttttaataa ctggcaaacc gaggaactct tggtattctt gccacgactc atctccatgc
     3901 agttggacga tatcaatgcc gtaatcattg accagagcca aaacatcctc cttaggttga
     3961 ttacgaaaca cgccaaccaa gtatttcgga gtgcctgaac tatttttata tgcttttaca
     4021 agacttgaaa ttttccttgc aataaccggg tcaattgttc tctttctatt gggcacacat
     4081 ataataccca gcaagtcagc atcggaatct agtgcacatt ctgcggcctc tgtgctctgc
     4141 aagccgcaaa ctttcaccaa tggaccagaa ctacctgtga aattaataac agacatactc
     4201 caagctgcct ttgtgtgctt aatcacgtat actcacgtgc tcaatagtca ccaatgccct
     4261 ccctcttggc cctctccttt tcttttttcg accgaattaa ttcttgaaga cgaaagggcc
     4321 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     4381 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4441 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4501 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4561 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4621 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4681 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4741 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4801 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4861 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4921 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4981 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5041 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5101 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5161 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5221 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5281 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5341 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5401 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5461 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5521 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5581 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5641 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     5701 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5761 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5821 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5881 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5941 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6001 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6061 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6121 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6181 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6241 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6301 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     6361 gcaggatccg ggatcgaaga aatgatggta aatgaaatag gaaatcaagg agcatgaagg
     6421 caaaagacaa atataagggt cgaacgaaaa ataaagtgaa aagtgttgat atgatgtatt
     6481 tggctttgcg gcgccgaaaa aacgagttta cgcaattgca caatcatgct gactctgtgg
     6541 cggacccgcg ctcttgccgg cccggcgata acgctgggcg tgaggctgtg cccggcggag
     6601 ttttttgcgc ctgcattttc caaggtttac cctgcgctaa ggggcgagat tggagaagca
     6661 ataagaatgc cggttggggt tgcgatgatg acgaccacga caactggtgt cattatttaa
     6721 gttgccgaaa gaacctgagt gcatttgcaa catgagtata ctagaagaat gagccaagac
     6781 ttgcgagacg cgagtttgcc ggtggtgcga acaatagagc gaccatgacc ttgaaggtga
     6841 gacgcgcata accgctagag tactttgaag aggaaacagc aatagggttg ctaccagtat
     6901 aaatagacag gtacatacaa cactggaaat ggttgtctgt ttgagtacgc tttcaattca
     6961 tttgggtgtg cactttatta tgttacaata tggaagggaa ctttacactt ctcctatgca
     7021 catatattaa ttaaagtcca atgctagtag agaagggggg taacacccct ccgcgctctt
     7081 ttccgatttt tttctaaacc gtggaatatt tcggatatcc ttttgttgtt tccgggtgta
     7141 caatatggac ttcctctttt ctggcaacca aacccataca tcgggattcc tataatacct
     7201 tcgttggtct ccctaacatg taggtggcgg aggggagata tacaatagaa cagataccag
     7261 acaagacata atgggctaaa caagactaca ccaattacac tgcctcattg atg
//
