LOCUS       40924_3361        7406 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAB707, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Murakami T, Burian J, Yanai K, Bibb MJ, Thompson CJ.
  TITLE     A system for the targeted amplification of bacterial gene clusters 
            multiplies antibiotic yield in Streptomyces coelicolor
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 108 (38), 16020-16025 (2011)
  PUBMED    21903924
REFERENCE   2  (bases 1 to 7406)
  AUTHORS   Murakami T, Burian J IV, Yanai K, Bibb MJ, Thompson CJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2011) Microbiology and Immunology, University of 
            British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, 
            Canada
REFERENCE   3  (bases 1 to 7406)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7406)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2011"; volume: "108"; issue: "38"; pages:
            "16020-16025"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-MAY-2011) Microbiology and Immunology, University of British 
            Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(107..127)
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     misc_feature    173..804
                     /label=derived from Streptomyces kanamyceticus
                     /note="derived from Streptomyces kanamyceticus"
     misc_recomb     503..527
                     /label=recombination site RsA
                     /note="recombination site RsA"
     protein_bind    1002..1035
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     oriT            complement(1042..1151)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             1467..2267
                     /codon_start=1
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILHDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     protein_bind    2277..2323
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_recomb     complement(2290..2323)
                     /label=FRT site
                     /note="FRT site"
     misc_feature    2343..4428
                     /label=derived from Streptomyces kanamyceticus
                     /note="derived from Streptomyces kanamyceticus"
     misc_recomb     4039..4066
                     /label=recombination site RsB
                     /note="recombination site RsB"
     promoter        complement(4712..4730)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4845..4949
                     /label=AmpR promoter
     CDS             4950..5807
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5981..6569
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    complement(6954..7088)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ctttttcctt tgtggtgtaa atagcaaagc aagcaagagt tctattacta aacacagcat
       61 gactcaaaaa acttagcaat tctgaaggaa agtccttggg gtcttctacc tttctcttct
      121 tttttggagg agtagaatgt tgagagtcag cagtagcctc atcatcacta gtgacttcaa
      181 cggcgtcgaa cccggtgaac cgcgcagcgc ccttgctgcg gaggagtccg cgggcacgga
      241 agaaggcgcg cttggcctcg cggttggcga gcagggaggc gacgggggca ccggcggctt
      301 cggccttcgc ctcgatggcg gcaatgaggg gcgcggcgtc gaccgcgacg gacttcccgc
      361 atccgtggcc gccggtaagg cggtactcaa cctgcccgtc ggaggggccg atcatggtca
      421 cccatcccat cggcccgctc aacccccgct ctcattcggt cagtttcagt cgtcttggct
      481 gattgtatcg tcacgaagtg acgatacctt ggtcctctca aatcaagatc atgatcaccg
      541 ggggaacctc ggacactcgg accggcttgg tcactcctgc ggatcaggct cctgaagcct
      601 gatgtcctgt tggactgtta cctctgtgcg aggtgcgtgg gctcaggtgc cgtacgtggc
      661 tgaggcggtg tgacgatccg atgtcggtgc ggtacctgcg cggggccggg tcgttggtcg
      721 gcgtatggag cacaccccgt atccggcggc ttcccgccgg ttcgccgagt tgtcgggccc
      781 gtactggatg tcctcggact ccccatccgg ccagttggcc ctggacgagg acggcaagct
      841 ggtcggcgag ggcgacgcgg cggcgcaggc ccaccaggtc ttcgagaacc tgcggcgctg
      901 cctgaccgcg gcgggcgcga cgttcgacga cgtcgtgaag ctgacgtact tcgtgacgga
      961 catggcgcac atgcattcgg ggatccgtcg acctgcagtt cgaagttcct attctctaga
     1021 aagtatagga acttcgaagt tcccgccagc ctcgcagagc aggattcccg ttgagcaccg
     1081 ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac
     1141 ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc
     1201 aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc
     1261 cgaagcaggg ttatgcagcg gaaaatgcag ctcacggtaa ctgatgccgt atttgcagta
     1321 ccagcgtacg gcccacagaa tgatgtcacg ctgaaaatgc cggcctttga atgggttcat
     1381 gtgcagctcc ataagcaaaa ggggatgata agtttatcac caccgactat ttgcaacagt
     1441 gccgttgatc gtgctatgat cgactgatgt catcagcggt ggagtgcaat gtcgtgcaat
     1501 acgaatggcg aaaagccgag ctcatcggtc agcttctcaa ccttggggtt acccccggcg
     1561 gtgtgctgct ggtccacagc tccttccgta gcgtccggcc cctcgaagat gggccacttg
     1621 gactgatcga ggccctgcgt gctgcgctgg gtccgggagg gacgctcgtc atgccctcgt
     1681 ggtcaggtct ggacgacgag ccgttcgatc ctgccacgtc gcccgttaca ccggaccttg
     1741 gagttgtctc tgacacattc tggcgcctgc caaatgtaaa gcgcagcgcc catccatttg
     1801 ccttcgcggc agcggggcca caggcagagc agatcatctc tgatccattg cccctgccac
     1861 ctcactcgcc tgcaagcccg gtcgcccgtg tccatgaact cgatgggcag gtacttctcc
     1921 tcggcgtggg acacgatgcc aacacgacgc tgcatcttgc cgagttgatg gcaaaggttc
     1981 cctatggggt gccgagacac tgcaccattc ttcatgatgg caagttggta cgcgtcgatt
     2041 atctcgagaa tgaccactgc tgtgagcgct ttgccttggc ggacaggtgg ctcaaggaga
     2101 agagccttca gaaggaaggt ccagtcggtc atgcctttgc tcggttgatc cgctcccgcg
     2161 acattgtggc gacagccctg ggtcaactgg gccgagatcc gttgatcttc ctgcatccgc
     2221 cagaggcggg atgcgaagaa tgcgatgccg ctcgccagtc gattggctga gctcataagt
     2281 tcctattccg aagttcctat tctctagaaa gtataggaac ttcgaagcag ctccagccta
     2341 cagcgatgaa gctcgtcgag atcgtctcgt ccgtgctcac cgcgcccgcc gccgtcgccg
     2401 cggtcaccga gctcgcgtac gccctgggca aggagcccgt cgcggtcggc gaccggcccg
     2461 gcttcgtcgc cgacgggctg ctcttcggct acctcaacca ggccgccgcg atgtacgagg
     2521 cgaagtacgc ctcccgcgag gacatcgacg ccgcgatgaa gctgggctgc ggcctgccga
     2581 tgggcccgct cgcgctgctc gacctgatcg gcgtggacac cgcccgcacg gtcctcgacg
     2641 ccatgtacgc cgagtcccac gaccgcctgc acgcccccgc gccggtcctc aagcagctca
     2701 gcgaggcggg cctgaccggg cgcaagtcgg ggcgcggctt ctacacgtac gccgagccgg
     2761 gcagcacgga ggtggtgcgc gacgcgctga ccccgccgga gggcgagcgg agcgtcccga
     2821 gccgtgccgt gcgcgcggtc ggtgtggcgg gctccggcac gatggcgtcc ggcatcgccg
     2881 aggtcttcgc caaggcgggc tacgacgtgg tgctcgccgc acgcagcgag gagaaggcgc
     2941 agctcgccag ggcccgtatc ggcaagtccc tcgcgcgctc ggtggagaag gggcggatca
     3001 ccgccgagtc ggcggcggag accctggagc ggatcacccc ggccggttcg tacgaggcgt
     3061 tcgccgatgt cgatctggcc ctggaggcca tcgcggagga cctggacgtc aagcagcagc
     3121 tgttcgcgac gctcgacaag gtctgcaagc ccggcgcgat cctggcgacc acgacgtcct
     3181 cgctgccggt cgtcgcctgc gcccgcgcca cctcgcgccc gcgtgacgtg atcggcatgc
     3241 acttcttcaa cccggccccg gcgatgaagc tggtcgaggt ggtgcgcacc gtgctgacct
     3301 cggacgaggt gcacgccacc gtccgtgagg tctgcgcggc gatccgcaag cacccggtgg
     3361 actgcggcga ccgcgcgggc ttcatcgtga acgcgctgct cttcccgtac ctgaacaacg
     3421 cgatcaagat ggtccaggag cactacgcca cgctggacga catcgacgcc gcgatgaagc
     3481 tgggcggcgg ctaccccatg gggcccttcg agctcctcga cgtggtcggc ctcgatgtct
     3541 cgctcgccat cgagaaggtc ctgcaccgcg agttccgcga cccgggcctg gccccggcgc
     3601 cgctgctcga acacctggtg gccgcgggct gcctcggccg caagacgggg cgtggcttcc
     3661 gcgaatatgc ccggcgctga gccggccgag gaccgcctgg actccgcgac ggactggggc
     3721 gggctgctcg aaccctcggg cggccctctc ccacacgagc accgtgctgc gcatatgcag
     3781 tacgttcgtg gcatgtccaa ggccgccaag tcctcacgca acgccagccc cgacgctccg
     3841 gagagcgccg cgggcagccg tgccgccgcc caacgcctca agatgcgcag ggagctggct
     3901 gcggcggcca tggagctctt cgcgaccaag gggtacgagg cgacgacggt cgacgagatc
     3961 gccgccgccg cgggcgtcgc ccgccggacc ttcttccggc acttccgctc caaggaagag
     4021 gcgatcttcc ccgaccacga cgacaccctg gtccgcgcgg aggaggtgct gaacgccgca
     4081 ccggcgcacg agcacccgct cgacacggtg tgccgcggca tcaaggaagt catgaagatg
     4141 tacgcggcct cgcccgcggt ctccgtggag cgctaccggc tgacccgtga ggtgcccacg
     4201 ctgcgcgagc gcgagatcgc ctccgtggcc cgctacgaac ggctgttcac gcggtatctg
     4261 ctcggccact tcgatgagca ggcgcaccac gacggcaacg acgacccgct gctcgccgag
     4321 gtcgccgcct ccgcggtggt caccgcccac aaccacgtcc tgcgccgctg gctgcgggcg
     4381 ggcggccagg gcgacctgga ggcccacctg aaccacgcgt tcggcatctt aaggagcagg
     4441 gaggggcccc ctcgacgggc tattaatcac cagtaccttc acgtggtcgt ccgggtgggt
     4501 cagggtctcc atcgccgcgc cgacctcccc gaagcccacc tccgccgtga tcagctccgc
     4561 cgcgtccacc ctgccgtccg cgatccgctc cagcgtgcgg gcgaattcct ccggcgggta
     4621 ctccatgcac atggtcaccg tgatgtcctt gcagatgccg accacggggc ggaagacgtc
     4681 ctccgtcatg caggcgccga tcagcaggat ccctttagtg agggttaatt gcggccgcga
     4741 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     4801 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4861 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4921 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4981 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5041 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5101 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5161 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     5221 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5281 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5341 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5401 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5461 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac
     5521 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5581 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5641 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5701 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5761 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5821 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5881 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5941 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6001 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6061 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6121 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6181 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6241 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6301 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6361 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6421 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6481 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6541 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6601 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6661 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6721 gcagcgagtc agtgagcgag gaagcggaag agcgctgact tccgcgtttc cagactttac
     6781 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag
     6841 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc
     6901 cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tcagatccag
     6961 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     7021 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     7081 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     7141 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctctagt
     7201 caaggcacta tacatcaaat attccttatt aaccccttta caaattaaaa agctaaaggt
     7261 acacaatttt tgagcatagt tattaatagc agacactcta tgcctgtgtg gagtaagaaa
     7321 aaacagtatg ttatgattat aactgttatg cctacttata aaggttacag aatatttttc
     7381 cataattttc ttgtatagca gtgcag
//
